Abstract:
:Immunocytochemical studies indicated that Purkinje cells cultured from chick embryonic cerebellum (embryonic day 8) strongly express a glutamate transporter EAAT2 cloned from human brain (GLT-1 in rat brain). At both 7 days and 14 days in culture, Purkinje neurons accumulated 1 microM [3H]L-glutamate via a potent "high-affinity" transport system that could be inhibited by D- and L-threo-3-hydroxyaspartate (D- and L-t-3OHA) and by L-trans-pyrrolidine-2,4-dicarboxylate (L-t-PDC). The order of potency of the three inhibitors was L-t-PDC approximately L-t-3OHA > D-t-30HA. Only the value of IC50 (concentration causing 50% inhibition) for D-t-3OHA significantly changed between 7 days (116 microM) and 14 days in culture (40 microM). All nH approximately 1, except in the case of the inhibition by D-t-3OHA at 14 days in culture (nH = 0.57), indicating the possible appearance of heterogeneity of the transport sites at later stages of culturing. Chronic inhibition of L-glutamate transport by L-t-PDC resulted in major changes in the morphology of Purkinje cells; particularly, the neurites almost completely regressed.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Meaney JA,Balcar VJ,Rothstein JD,Jeffrey PLdoi
10.1002/(SICI)1097-4547(19981201)54:5<595::AID-JNRsubject
Has Abstractpub_date
1998-12-01 00:00:00pages
595-603issue
5eissn
0360-4012issn
1097-4547pii
10.1002/(SICI)1097-4547(19981201)54:5<595::AID-JNRjournal_volume
54pub_type
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