Impact of chemokines on the properties of spinal cord-derived neural progenitor cells in a rat spinal cord lesion model.


:The existence of endogenous neural progenitor cells (NPCs) in the adult spinal cord (sc) provides the potential for tailored repair therapies after spinal cord injury (SCI). This study investigates the impact of inflammatory mediators on properties of NPC cultures derived from adult rats after SCI. The Infinite Horizon impactor was used to apply 200-kdyn thoracic sc lesions in adult rats. Control groups received laminectomies to equivalent sc regions. Thoracic sc segments were taken for neurosphere cell cultures. Cell proliferation was found to be significantly higher in lesion groups. Neurosphere-derived cells differentiated into neurons, oligodendroglia, and astroglia. Lesion cultures exhibited significantly higher amounts of glial fibrillary acidic protein (GFAP) mRNA (P < 0.0005) and β-III-tubulin mRNA (P < 0.05) compared with sham animals. Neurospheres from different treatment groups exhibited the same amounts of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 mRNA. C-C chemokine receptor (CCR) expression on neurospheres was examined by real-time RT-PCR. CCR1 was expressed most consistently in mRNA levels in neurospheres from both treatment groups. After cell differentiation, CCR1 mRNA amounts decreased. CCR1 was detectable by immunohistochemistry in neurospheres and differentiated cells of both groups. Application of CCL3 during differentiation cycles led to significantly higher GFAP mRNA amounts in sham animals compared with CCL3-free cultures; in contrast, CCL3 had no impact on cell differentiation in the lesion group. In conclusion, impact SCI alters differentiation tendencies and proliferation rates of adult-derived sc NPCs. Thereby, CCR1/CCL3 promotes specifically astroglial differentiation of NPCs, which provides a potential target for future neurorestorative approaches.


J Neurosci Res


Knerlich-Lukoschus F,Krossa S,Krause J,Mehdorn HM,Scheidig A,Held-Feindt J




Has Abstract


2015-04-01 00:00:00












  • Functional properties of neurons derived from fetal mouse neurospheres are compatible with those of neuronal precursors in vivo.

    abstract::Neural stem cells can be propagated in culture as neurospheres, yielding neurons and glial cells upon differentiation. Although the neurosphere model is widely used, the functional properties of the neurosphere-derived neurons have been only partially characterized, and it is unclear whether repeated passaging alters ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


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  • Effects of neural stem cells on synaptic proteins and memory in a mouse model of Alzheimer's disease.

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    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Zhang W,Wang GM,Wang PJ,Zhang Q,Sha SH

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  • Uptake, release, and binding of taurine in degenerated rat retinas.

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    journal_title:Journal of neuroscience research

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


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    更新日期:2007-10-01 00:00:00

  • Combination therapy of normobaric oxygen with hypothermia or ethanol modulates pyruvate dehydrogenase complex in thromboembolic cerebral ischemia.

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    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


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    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


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  • Calcitonin gene-related peptide regulation of glial cell-line derived neurotrophic factor in differentiated rat myotubes.

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    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Rosa E,Cha J,Bain JR,Fahnestock M

    更新日期:2015-03-01 00:00:00

  • Time-restricted feeding prevents depressive-like and anxiety-like behaviors in male rats exposed to an experimental model of shift-work.

    abstract::Individuals who regularly shift their sleep timing, like night and/or shift-workers suffer from circadian desynchrony and are at risk of developing cardiometabolic diseases and cancer. Also, shift-work is are suggested to be a risk factor for the development of mood disorders such as the burn out syndrome, anxiety, an...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


    authors: Guerrero-Vargas NN,Zárate-Mozo C,Guzmán-Ruiz MA,Cárdenas-Rivera A,Escobar C

    更新日期:2021-02-01 00:00:00

  • Neuroanatomical distribution and functions of brain-derived neurotrophic factor in zebrafish (Danio rerio) brain.

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    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审


    authors: Anand SK,Mondal AC

    更新日期:2020-05-01 00:00:00

  • Glutamate transport in cultures from developing avian cerebellum: presence of GLT-1 immunoreactivity in Purkinje neurons.

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    journal_title:Journal of neuroscience research

    pub_type: 杂志文章


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    更新日期:1998-12-01 00:00:00