Abstract:
:The existence of endogenous neural progenitor cells (NPCs) in the adult spinal cord (sc) provides the potential for tailored repair therapies after spinal cord injury (SCI). This study investigates the impact of inflammatory mediators on properties of NPC cultures derived from adult rats after SCI. The Infinite Horizon impactor was used to apply 200-kdyn thoracic sc lesions in adult rats. Control groups received laminectomies to equivalent sc regions. Thoracic sc segments were taken for neurosphere cell cultures. Cell proliferation was found to be significantly higher in lesion groups. Neurosphere-derived cells differentiated into neurons, oligodendroglia, and astroglia. Lesion cultures exhibited significantly higher amounts of glial fibrillary acidic protein (GFAP) mRNA (P < 0.0005) and β-III-tubulin mRNA (P < 0.05) compared with sham animals. Neurospheres from different treatment groups exhibited the same amounts of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 mRNA. C-C chemokine receptor (CCR) expression on neurospheres was examined by real-time RT-PCR. CCR1 was expressed most consistently in mRNA levels in neurospheres from both treatment groups. After cell differentiation, CCR1 mRNA amounts decreased. CCR1 was detectable by immunohistochemistry in neurospheres and differentiated cells of both groups. Application of CCL3 during differentiation cycles led to significantly higher GFAP mRNA amounts in sham animals compared with CCL3-free cultures; in contrast, CCL3 had no impact on cell differentiation in the lesion group. In conclusion, impact SCI alters differentiation tendencies and proliferation rates of adult-derived sc NPCs. Thereby, CCR1/CCL3 promotes specifically astroglial differentiation of NPCs, which provides a potential target for future neurorestorative approaches.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Knerlich-Lukoschus F,Krossa S,Krause J,Mehdorn HM,Scheidig A,Held-Feindt Jdoi
10.1002/jnr.23527subject
Has Abstractpub_date
2015-04-01 00:00:00pages
562-71issue
4eissn
0360-4012issn
1097-4547journal_volume
93pub_type
杂志文章abstract::Cortical or total brain cultures of microglia are commonly used as a model to study the inflammatory processes in Parkinson's disease. Here we characterize microglia cultures from rat ventral midbrain and evaluate their response to zymosan A. We used specific markers of microglia and evaluated the morphology, the phag...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22219
更新日期:2010-02-15 00:00:00
abstract::Acoustic overstimulation causes apoptotic cell death in the cochlea. This death process is mediated, in part, by the mitochondrial signaling pathway involving Bcl-2 family proteins. Myeloid cell leukemia sequence 1 (Mcl-l) is an antiapoptotic member of the Bcl-2 family. Its involvement in noise-induced hair cell death...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22333
更新日期:2010-06-01 00:00:00
abstract::In this report, we address the molecular mechanisms that regulate axonal growth by focusing on the gene for one of the major axonal cytoskeletal proteins, T alpha1 alpha-tubulin. During the developmental growth of sympathetic neurons, transcription of a beta-galactosidase transgene driven by the T alpha1 promoter (T a...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1997-06-01 00:00:00
abstract::A new subdivision with distinctive morphological and functional attributes has been identified at the caudomedial margin of the neostriatum and surrounding the rostrolateral border of the globus pallidus in the brains of the rat, cat, monkey, and human. The subdivision is termed marginal division (MrD) based on its lo...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1999-10-15 00:00:00
abstract::The effect of two different potassium channel blockers, 4-aminopyridine (4-AP) and quinine, on the alpha 2-adrenoceptor mediated modulation of norepinephrine (NE) release was investigated. Pairs of mouse vasa deferentia were loaded with 3H-norepinephrine (3H-NE), superfused continuously, and stimulated electrically. 4...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490200114
更新日期:1988-05-01 00:00:00
abstract::The role of axons in the expression of the major myelin glycoprotein, P0, has been investigated using neuron/Schwann cell cultures. These cultures were either nonmyelinating or myelinating due to growth in defined medium or in medium containing serum and chick embryo extract, respectively. The neurons and Schwann cell...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490260202
更新日期:1990-06-01 00:00:00
abstract::With the use of an antiserum directed against the human smooth muscle-associated protein 8 (SMAP8) fragment SMAP8(98-138), Western blot and immunohistochemical studies revealed SMAP8 expression in the rat brain. A band with a molecular size of about 45 kDa was detected in tissues from the rat hypothalamus and a weaker...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21315
更新日期:2007-06-01 00:00:00
abstract::Niemann-Pick C (NPC) disease is an autosomal recessive lipidosis characterized by lysosomal accumulations of unesterified cholesterol. Cultured NPC cells exhibit a defect in the intracellular trafficking of LDL-derived cholesterol that leads to lysosomal accumulations of unesterified cholesterol. We found in a prelimi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(sici)1097-4547(19970801)49:3<389::aid-jnr
更新日期:1997-08-01 00:00:00
abstract::Despite the large number of growth factors shown to affect dopaminergic cell survival and differentiation in vitro, presently little is known about the role these growth factors play during normal in vivo development of dopaminergic neurons. To address this issue, glia and neurons of both the mesencephalic home region...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19980815)53:4<405::AID-JNR
更新日期:1998-08-15 00:00:00
abstract::Pigment epithelium-derived factor (PEDF) is a neurotrophic protein synthesized and secreted by retinal pigment epithelial (RPE) cells in early embryogenesis and has been shown to be present in the extracellular matrix between the RPE cells and the neural retina. It induces neuronal differentiation and promotes surviva...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1999-09-15 00:00:00
abstract::The Rho GTPases are important regulators of neurite outgrowth and pathfinding. We have recently reported that a Rho-family guanine nucleotide exchange factor, GEFT, modulates dendrite spine morphology and basal neurite outgrowth in primary hippocampal neurons and Neuro2A cells, respectively. Here we demonstrate that G...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20814
更新日期:2006-05-15 00:00:00
abstract::In many regions of the rat central nervous system, oligodendrocytes develop from migratory A2B5+ precursor cells. In the rat spinal cord, during early embryonic development the capacity for oligodendrogenesis appears to be restricted to ventral regions of the spinal cord, while cultures of postnatal rat spinal cord co...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490370208
更新日期:1994-02-01 00:00:00
abstract::Synaptic vesicles were isolated from adult bovine cortical gray matter by differential centrifugation and membrane filtration of a hypoosmotically lysed crude mitochondrial fraction. Vesicle preparations were analyzed for purity by electron microscopy and enzyme assays. Polyacrylamide gel electrophoresis of SDS-solubi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490030204
更新日期:1977-01-01 00:00:00
abstract::Exogenously added gangliosides are known to promote neurite outgrowth in a variety of cell types, including some neuroblastoma cell lines. To study neuritogenesis in SH-SY5Y human neuroblastoma we serum starved the cells for 24 hr and exposed them to gangliosides (GM1, GM3, or GT1b), platelet-derived growth factor (PD...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(sici)1097-4547(19970315)47:6<617::aid-jnr
更新日期:1997-03-15 00:00:00
abstract::We describe here the presence of ubiquitin and its conjugation system in the rat retina. Retinal homogenates and supernatants conjugate [125I]human ubiquitin with either endogenous or exogenous proteins. The conjugating activity is relatively stable over time, requires ATP, and has a pH optimum of approximately 8. The...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490300220
更新日期:1991-10-01 00:00:00
abstract::Transection of the hindlimb dorsal column fibers leads to a partial deafferentation of the neurons of the nucleus gracilis, the first relay of the ascending dorsal column-medial lemniscal (DC-ML) neural network. In response to the deafferentation, a synaptic renewal cycle is initiated and the neurons of the nucleus gr...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490400409
更新日期:1995-03-01 00:00:00
abstract::Glial and glia-derived cells express a variety of receptors for neurotransmitters and hormones, the majority of which evoke both Ca(2+) release from intracellular stores and Ca(2+) entry across the plasma membrane. We investigated the links between histamine H(1) receptor activation, Ca(2+) release from intracellular ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21784
更新日期:2008-11-15 00:00:00
abstract::Laminin mediates neural adhesion and process formation. A possible signal transduction pathway for laminin was investigated in both NG108-15 and PC12 neuronal cells using radiolabeling studies as well as various stimulators and inhibitors of phosphatases and kinases. Using [32P]-ortho-phosphate, laminin caused a decre...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490270321
更新日期:1990-11-01 00:00:00
abstract::To examine the role of platelet-derived growth factor (PDGF) in the in vivo regulation of Schwann cell proliferation, steady-state levels of mRNAs encoding PDGF A and B chains, and PDGF alpha and beta receptors were measured in immature and adult rat sciatic nerves and in cultured rat Schwann cells. PDGF B chain and P...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490310206
更新日期:1992-02-01 00:00:00
abstract::Previous studies have demonstrated variability in the phenotype of rat C6 glioma cells. In the present study, we compared morphology, growth rate, and beta-adrenergic regulation of gene expression in early (P39-47) and late (P55-90) passage C6 cells. Morphological changes were observed in five independently derived, l...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490330213
更新日期:1992-10-01 00:00:00
abstract::Living organotypic cocultures of rat thalamic and cortical explants were used to examine the effects of blocking action potential activity on the morphological development of axons in the mammalian neocortex. Studies in vivo have suggested that blocking sodium channel-dependent activity influences the growth character...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19960315)43:6<707::AID-JNR
更新日期:1996-03-15 00:00:00
abstract::Traumatic brain injury (TBI) is the major cause of physical disability and emotional vulnerability. Treatment of TBI is lacking due to its multimechanistic etiology, including derailed mitochondrial and cellular energy metabolism. Previous studies from our laboratory show that an endogenous nitric oxide (NO) metabolit...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.24279
更新日期:2018-12-01 00:00:00
abstract::Recent experiments have shown that Arg, Lys, and Leu can be incorporated posttranslationally into proteins of regenerating sciatic nerves of rats. The present experiments investigate a mixture of 15 radioactive amino acids to determine if additional amino acids can be conjugated posttranslationally to proteins of rege...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490250407
更新日期:1990-04-01 00:00:00
abstract::After hemisection of the spinal cord in rats, alterations in amino acid incorporation into protein have been observed in brain and spinal cord. Proteins mediating these changes have been studied in the present experiment. Male, Long-Evans hooded rats were given either a laminectomy and dura incision (sham) at spinal s...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490050108
更新日期:1980-01-01 00:00:00
abstract::The number of older people who are suffering from memory impairment is increasing among populations throughout the world. Alzheimer's disease (AD) affects about 5% of people over 65 years old. The hippocampus, a brain area critical for learning and memory, is especially vulnerable to damage in the early stages of AD. ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23319
更新日期:2014-04-01 00:00:00
abstract::Adhesion responses triggered by integrin-class matrix receptors have been implicated in the synaptic reorganization events necessary for certain types of neuronal plasticity. Hippocampal slice cultures were used to test whether the related structural transformations elicited by NMDA receptor stimulation are regulated ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(20000315)59:6<827::AID-JNR
更新日期:2000-03-15 00:00:00
abstract::Autonomic neuropathy in several neurodegenerative disorders results from disturbance in physiological functions of different cell types in the central and peripheral nervous systems. For a clearer understanding of the etiology and pathogenesis of the autonomic disorders it is necessary to create animal models in which...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19980115)51:2<162::AID-JNR
更新日期:1998-01-15 00:00:00
abstract::A distinct population of white matter progenitor cells (WMPCs), competent but not committed to generate oligodendrocytes, remains ubiquitous in the adult human subcortical white matter. These cells are present in both sexes and into senescence and may constitute as much as 4% of the cells of adult human capsular white...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10397
更新日期:2002-09-15 00:00:00
abstract::Brain cathepsin D, purified by affinity chromatography on Sepharose pepstatin columns, was incubated with synthetic peptides corresponding to the susceptible regions of the myelin basic protein encompassing the two Phe-Phe bonds. One peptide, Leu-Gly-Arg-Phe-Phe-Gly-Gly, was cleaved by cathepsin D at the Phe-Phe bond ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490050306
更新日期:1980-01-01 00:00:00
abstract::Noradrenalin (NA) regulates the expression of arginine-vasopressin (AVP) and oxytocin (OT) by magnocellular neurons in the supraoptic nucleus (SON) of the hypothamalus. Nitric oxide (NO) may be one of the factors involved in the NA signaling pathway regulating AVP and OT expression. To test this possibility, we used a...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21394
更新日期:2007-10-01 00:00:00