Abstract:
:With the use of an antiserum directed against the human smooth muscle-associated protein 8 (SMAP8) fragment SMAP8(98-138), Western blot and immunohistochemical studies revealed SMAP8 expression in the rat brain. A band with a molecular size of about 45 kDa was detected in tissues from the rat hypothalamus and a weaker band from the cortex. SMAP8 immunoreactivity (irSMAP8) was detected in neurons of the hypothalamic paraventricular, supraoptic, and supraoptic retrochiasmatic nuclei; a few irSMAP8 cells were scattered in the zona incerta as well as the cerebral cortex. Immunoreactive cell processes were detected mostly in the internal layer of the median eminence. Double labeling the hypothalamic sections with SMAP8 and vasopressin (VP) or oxytocin (OT) antiserum revealed that a population of VP- and OT-immunoreactive neurons expressed irSMAP8. The biological activity of SMAP8 in rat central neurons was assessed by the calcium microfluorimetric Fura-2 method. SMAP8 (100 nM) elevated cytosolic calcium concentrations [Ca2+]i in a population of dissociated and cultured rat hypothalamic neurons; the response was eliminated in Ca2+-free saline. This is the first evidence of irSMAP8 in a population of VP/OT-containing hypothalamic neurons in the rat, and the peptide is biologically active in hypothalamic neurons, as evidenced by mobilization of extracellular Ca2+.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Brailoiu GC,Dun SL,Mizuo K,Brailoiu E,Yang J,Chang JK,Dun NJdoi
10.1002/jnr.21315subject
Has Abstractpub_date
2007-06-01 00:00:00pages
1789-96issue
8eissn
0360-4012issn
1097-4547journal_volume
85pub_type
杂志文章abstract::The use of trimethadione (TMO) as a protector in hyperbaric oxygen toxicity in the conscious rat has been examined in detail. The oxidation-reduction state of pyridine nucleotides was measured simultaneously with the EEG activity from the surface of the brain cortex. From the data obtained, a few parameters were calcu...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490010205
更新日期:1975-01-01 00:00:00
abstract::It is generally recognized that lipid peroxides play an important role in the pathogenesis of several diseases and that sulfhydryl groups are critically involved in cellular defense against endogenous or exogenous oxidants. Recent evidence indicates that lipid peroxides directly participate in induction of cytoprotect...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10177
更新日期:2002-04-01 00:00:00
abstract::We have developed a silicone nerve regeneration chamber that is partitioned into two compartments by a strip of nitrocellulose paper. The modified two-compartment chamber allows the investigation of the effects on rat sciatic nerve regeneration of trophic or growth factors that are initially bound to the nitrocellulos...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490200306
更新日期:1988-07-01 00:00:00
abstract::GM1, GD1a, and GT1b inhibit both PDGF-stimulated and serum-stimulated DNA synthesis in Swiss 3T3 cells and the human glioma cell line U-1242 MG in a dose-dependent manner. The ganglioside inhibitory effect is counteracted in a dose-responsive fashion by serum such that ganglioside-induced inhibition is essentially abo...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490410408
更新日期:1995-07-01 00:00:00
abstract::After left unilateral cochlear ablation (UCA) in young adult guinea pigs, the appearance of plasticities in auditory pathways suggested altered gene expression and modified phenotypic behaviors of auditory neurons. Because phosphorylated cyclic-AMP response element-binding protein (CREB-P) is a transcription factor th...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20820
更新日期:2006-05-15 00:00:00
abstract::Brain zinc homeostasis is strictly controlled under healthy conditions, indicating the importance of zinc for physiological function in the brain. A part of zinc in the brain exists in the synaptic vesicles, is released from a subclass of glutamatergic neurons (i.e., zincergic neurons), and serves as a signal factor (...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.23385
更新日期:2014-07-01 00:00:00
abstract::Photopic L-type responses were recorded from the soma and the axon terminal of horizontal cells of the catfish (Ictalurus punctatus) retina in eye-cup preparations. The responses were produced by a spot of light with 100-micron diameter (intensity, 10 microW/cm2), which was flashed or steadily illuminated and swept al...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490110405
更新日期:1984-01-01 00:00:00
abstract::Immature and mature rat astroglia in culture were assayed for glutamine synthetase (GS) activity after a single exposure to the epileptogen FeCl2. Cells were cultured with both standard and elevated extracellular potassium or glutamate (Glu) concentrations. FeCl2 reduced GS activity below control levels, whereas high ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490240408
更新日期:1989-12-01 00:00:00
abstract::We suggest that a dysregulation of energy metabolism in the brain of genetic absence epilepsy rats from Strasbourg (GAERS) could create a specific cerebral environment that would favor the expression of spike-and-wave discharges (SWD) in the thalamocortical loop, largely dependent on glutamatergic and gamma-aminobutyr...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10086
更新日期:2001-12-01 00:00:00
abstract::Damage to the lateral nucleus of the septum in the rat resulted in atypical learning to a previously meaningless stimulus. Nonreinforced preexposure to a stimulus normally makes more difficult subsequent use of that stimulus for learning. The effect of this preexposure on learning is termed latent inhibition and was d...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490040309
更新日期:1979-01-01 00:00:00
abstract::Human fetal brain is a potential source of neural stem cells (NSCs) for cell replacement therapy in neurodegenerative diseases. We explored whether NSCs isolated from cortex and striatum of human fetuses, aged 6-9 weeks post-conception, maintain their regional identity and differentiate into specific neuron types in c...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21066
更新日期:2006-12-01 00:00:00
abstract::The recent recognition that the p75 neurotrophin receptor, p75((NTR)), can induce apoptotic signals has contributed to the perception that it acts primarily as a death receptor. Although the molecular mechanisms of p75(NTR) signaling remain to be fully characterized, many of the currently identified pathways activated...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/1097-4547(20000801)61:3<237::AID-JNR1>3.0.
更新日期:2000-08-01 00:00:00
abstract::The structures associated with learning and memory have been widely studied for over 100 years. The idea of the famous neuropsychologist K.S. Lashley, that learning and memory are stored diffusely in the brain, dominated neuroscience in the early half of Twentieth Century. Since Scoville reported in 1957 a persistent ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.10545
更新日期:2003-03-01 00:00:00
abstract::The existence of endogenous neural progenitor cells (NPCs) in the adult spinal cord (sc) provides the potential for tailored repair therapies after spinal cord injury (SCI). This study investigates the impact of inflammatory mediators on properties of NPC cultures derived from adult rats after SCI. The Infinite Horizo...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23527
更新日期:2015-04-01 00:00:00
abstract::A link between aluminum (Al) exposure and age-related neurological disorders has long been proposed. Although the exact mechanism by which the metal may influence disease processes is unknown, there is evidence that exposure to Al causes an increase in both oxidative stress and inflammatory events. These processes hav...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10877
更新日期:2004-02-15 00:00:00
abstract::Corticosterone (CORT), one of the glucocorticoids, causes neuronal damage in the hippocampus, but the mechanism(s) of action underlying its effects remains unknown. Brain-derived neurotrophic factor (BDNF) is a neurotrophic factor that belongs to the neurotrophin family, affects the survival and/or differentiation of ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19990715)57:2<227::AID-JNR
更新日期:1999-07-15 00:00:00
abstract::An experimental model based on kainic acid (KA) injections replicates many phenomenological features of human temporal lobe epilepsy, the most common type of epilepsy in adults. Taurine, 2-aminoethanesulfonic acid, present in high concentrations in many invertebrate and vertebrate systems, is believed to serve several...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21950
更新日期:2009-05-01 00:00:00
abstract::The minimal requirements for the regeneration of optic nerve fibers in vitro were established in a serum-free retinal explant preparation. This serum-free preparation was developed as a prerequisite for testing the growth-promoting activity of tissue extracts prepared from the primary target of regenerating fibers. Ex...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490080221
更新日期:1982-01-01 00:00:00
abstract::We have performed proteomic analysis in the cerebrospinal fluid in an animal model of Parkinson's disease induced by axotomy of the medial forebrain bundle. In this model, the degeneration of dopaminergic neurons was completed in 14 days, with a loss of about 50% dopaminergic neurons in the substantia nigra and a loss...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21452
更新日期:2007-12-01 00:00:00
abstract::Despite the accumulating data on the molecular and cell biological characteristics of neural stem/progenitor cells, their electrophysiological properties are not well understood. In the present work, changes in the membrane properties and current profiles were investigated in the course of in vitro-induced neuron form...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21282
更新日期:2007-06-01 00:00:00
abstract::Spinal cord injury (SCI) triggers chronic intraspinal inflammation consisting of activated resident and infiltrating immune cells (especially microglia/macrophages). The environmental factors contributing to this protracted inflammation are not well understood; however, myelin lipid debris is a hallmark of SCI. Myelin...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24114
更新日期:2018-06-01 00:00:00
abstract::The distribution of neuronal intermediate filament proteins in the developing mouse olfactory bulb and olfactory epithelium was characterized by immunocytochemical approach. Antibodies against alpha-internexin, neurofilament triplet proteins (NFTPs; NF-L, NF-M, and NF-H) and peripherin were used to determine their exp...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19981101)54:3<353::AID-JNR
更新日期:1998-11-01 00:00:00
abstract::The GTP binding proteins Rhes and AGS1/Dexras1 define a subfamily of the Ras superfamily and have been shown to affect signaling by G-protein-coupled receptors. We tested the effects of both proteins at an early stage of signaling by dopamine receptors, activation of adenylyl cyclase. Rhes decreased dopamine D1 recept...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22604
更新日期:2011-06-01 00:00:00
abstract::Forty-seven rats were cannulated permanently in either the globus pallidus or neostriatum and subsequently injected with carbachol, scopolamine, or carbachol plus scopolamine. The injection of carbachol (1 mug) into the globus pallidus produced transient hyperkinetic phasic dyskinesias (84%) followed in some cases by ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490010206
更新日期:1975-01-01 00:00:00
abstract::Transforming growth factor-β1 (TGF-β1) is an important member of multifunctional growth factor superfamily. It has been implicated in pain signaling, but little is known about the underlying mechanisms. Herein, we report that TGF-β1 can exert a sustained enhancing effect on the functional activity of acid-sensing ion ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.24481
更新日期:2019-10-01 00:00:00
abstract::An aminopeptidase purified to homogeneity from cytosol of rat brain cleaved dynorphins having 5-17 residues and selected proenkephalins at the Tyr-Gly bond only to release Tyr and the desTyr fragments. The enzyme protein consisted of a single polypeptide chain of Mr 103,000 and was inhibited by puromycin, bestatin, an...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490110311
更新日期:1984-01-01 00:00:00
abstract::Sphingolipidoses are a class of inherited diseases that result from the toxic accumulation of undigested sphingolipids in lysosomes and other cellular membranes. Sphingolipids are particularly enriched in cells of the nervous system, and their excessive accumulation during disease has a significant impact on the nervo...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.23763
更新日期:2016-11-01 00:00:00
abstract::Ex vivo gene therapy is emerging as a promising approach for the treatment of neurodegenerative diseases and central nervous system (CNS) trauma. We have shown previously that human adult astrocytes can be expanded in vitro and can express various therapeutic transgenes (Ridet et al. [1999] Hum. Gene Ther. 10:271-280;...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10617
更新日期:2003-06-15 00:00:00
abstract::Neuronal progenitors in the adult hippocampus continually proliferate and differentiate to the neuronal lineage, and ischemic insult promotes hippocampal neurogenesis. However, newborn neurons show a progressive reduction in numbers during the initial few weeks, therefore, enhanced survival of newborn neurons seems to...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21036
更新日期:2006-11-01 00:00:00
abstract::Neuroepithelial b2T cells were derived from the hindbrain and the spinal cord of mouse transgenic embryos, which expressed SV40 T antigen under the control of a Hoxb2 enhancer. Strikingly, b2T cell lines of either origin exhibit a very similar gene expression pattern, including markers of the hindbrain and the spinal ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10716
更新日期:2003-09-15 00:00:00