Abstract：
:We have developed a silicone nerve regeneration chamber that is partitioned into two compartments by a strip of nitrocellulose paper. The modified two-compartment chamber allows the investigation of the effects on rat sciatic nerve regeneration of trophic or growth factors that are initially bound to the nitrocellulose partition. In this study we compared the effects of untreated nitrocellulose, a siliconized nitrocellulose strip, and a strip that had been soaked in a basic fibroblast growth factor (FGF) solution. FGF is a known angiogenic factor and a mitogen for endothelial cells, fibroblasts, and Schwann cells. All of these cell types are present in the peripheral nerve. In vitro analyses, using 3T3 cells as test cells, showed that some of the bound FGF remained active on the nitrocellulose paper for at least 8-10 days. In vivo experiments, examined at 16 days post-implantation, revealed that spatial migration of all cellular elements (perineurial-like cells, vasculature, and Schwann cells) across the chamber gap was slower with untreated nitrocellulose strips than with siliconized strips but was most advanced with FGF-treated ones. Most striking was the well-developed vascular arborization of the regenerate within the FGF chambers. Histologic sections from the proximal one-half of the chamber revealed that the regenerate in untreated strip chambers consisted of fibrin matrix and erythrocytes, whereas a well-developed structure with all the cellular elements of a regenerating nerve was seen in several of the FGF strip chambers. We conclude that FGF stimulates peripheral nerve regeneration in this model.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Danielsen N,Pettmann B,Vahlsing HL,Manthorpe M,Varon Sdoi
10.1002/jnr.490200306subject
Has Abstractpub_date
1988-07-01 00:00:00pages
320-30issue
3eissn
0360-4012issn
1097-4547journal_volume
20pub_type
杂志文章abstract：:Preterm birth and associated brain injury are the primary cause of cerebral palsy and developmental disabilities and are among the most serious global health issues that modern society faces. Current therapy for infants suffering from premature brain injury is still mainly supportive, and there are no effective treatm...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.24548
更新日期：2020-05-01 00:00:00
abstract：:Brain zinc homeostasis is strictly controlled under healthy conditions, indicating the importance of zinc for physiological function in the brain. A part of zinc in the brain exists in the synaptic vesicles, is released from a subclass of glutamatergic neurons (i.e., zincergic neurons), and serves as a signal factor (...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.23385
更新日期：2014-07-01 00:00:00
abstract：:Embryonic central nervous system neuroepithelial cells are a transient population of cells that give rise to neuronal and glial progenitors. In the E12-E16 embryonic rat spinal neural tube we have identified neuroepithelial cells as radially oriented cells expressing the GD3 ganglioside as recognized by the monoclonal...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19960901)45:5<549::AID-JNR
更新日期：1996-09-01 00:00:00
abstract：:Growth hormone (GH) appears to play a major role in a reciprocal axis that has been postulated between the thymus and pituitary glands. Our previous studies showed that thymic structure, as well as T-cell proliferation and IL-2 synthesis, could be restored in aged female Wistar-Furth rats by the implantation of GH3 pi...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490180118
更新日期：1987-01-01 00:00:00
abstract：:Disturbances in neuronal calcium homeostasis have been implicated in a variety of neuropathological conditions, including cerebral ischemia, hypoglycemia, and epilepsy, and possibly constitute part of the cell death process associated with chronic neurodegenerative disorders. We investigated if endoplasmic reticulum (...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20631
更新日期：2005-10-15 00:00:00
abstract：:The mammalian genome contains both constitutively expressed and heat-shock-inducible members of the hsp70 gene family. Riboprobes derived from members of these two classes of heat shock genes were utilized in Northern blot studies to analyze brain mRNA isolated from control rabbits and rats and from animals subjected ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490250103
更新日期：1990-01-01 00:00:00
abstract：:Several neuronal and non-neuronal cell lines express a Ca(2+)-dependent mechanism of transmitter release that can be demonstrated after loading the cells with acetylcholine during culture. In contrast, a particular cell line, the neuroblastoma N18TG-2, was found to be deficient for release. We transfected N18TG-2 cell...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19960801)45:3<195::AID-JNR
更新日期：1996-08-01 00:00:00
abstract：:The presence of acetylcholinesterase has been detected in the thymus of several species both biochemically and histochemically. In this study we have investigated the molecular forms and the level of this enzyme in separate compartments of the murine thymus and in different thymocyte subpopulations. Similar levels of ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490290210
更新日期：1991-06-01 00:00:00
abstract：:The presence of oligodendrocyte precursor cells which cannot be removed from primary cultures by antibody-dependent techniques complicates the interpretation of transplantation experiments designed to examine the potential of astrocytes to influence remyelination (Blakemore et al.; Glia 13:79-91, 1995). In the present...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
abstract：:Glycolysis was blocked in the hippocampus of the otherwise unaffected rabbit by perfusion of a previously implanted thin dialysis tube with a medium containing 0.1 mM iodoacetate. The perfusate from the dialysis tube was analyzed for free amino acids with liquid chromatographic separation of ortophthaldialdehyde deriv...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490130403
更新日期：1985-01-01 00:00:00
abstract：:Synaptic circuitry in the upper dorsal horn, disorganized due to transganglionic degenerative atrophy (evoked by blockade of retrograde axoplasmic transport in the related peripheral nerve), begins to be reestablished by a process of regenerative synaptoneogenesis as soon as retrograde transport is resumed. Central ax...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490220110
更新日期：1989-01-01 00:00:00
abstract：:The cytokines ciliary neurotrophic factor (CNTF) and leukemia inhibitory factor (LIF) signal through a receptor complex formed between two transmembrane proteins, gp130 and LIFRbeta. In addition, CNTF also uses a ligand-binding component which is anchored to the cell membrane. In the case of cardiotrophin-1 (CT-1), LI...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19990101)55:1<119::AID-JNR
更新日期：1999-01-01 00:00:00
abstract：:Neural stem cells can be propagated in culture as neurospheres, yielding neurons and glial cells upon differentiation. Although the neurosphere model is widely used, the functional properties of the neurosphere-derived neurons have been only partially characterized, and it is unclear whether repeated passaging alters ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20835
更新日期：2006-06-01 00:00:00
abstract：:The response of cells localized in the brain subventricular zone (SVZ) to growth factor stimulation has been largely described for development and adult life, whereas no information on their behavior during aging is available. To address the question of whether the cells in the SVZ of old mice respond to the intracere...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10614
更新日期：2003-06-01 00:00:00
abstract：:Direct brain injections of the N-methyl-D-aspartate receptor agonist quinolinic acid (QA) trigger an excitotoxic cascade characterized by rapid neuronal death and glial/immune cell activation. The present study compared the timing of immediate early gene (IEG; c-fos, c-jun, jun-B, and zif/268) induction with the respo...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490360511
更新日期：1993-12-01 00:00:00
abstract：:Previous studies have shown that PC12 cells overexpressing beta/A4 amyloid peptide display altered morphology characterized by pronounced membrane ruffling and extensive intercellular appositions. Having observed other cell types in which these features accompany increased connexin43 (Cx43) production and gap junction...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19960415)44:2<124::AID-JNR
更新日期：1996-04-15 00:00:00
abstract：:Calcium/calmodulin-dependent serine kinase (CASK), a causative gene in X-linked mental retardation, carries out multiple functions in neurons, including vesicle trafficking of ion channels, synapse formation, and gene transcription. From a yeast two-hybrid screen, Krüppel-like zinc finger protein B cell lymphoma/COUP-...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22407
更新日期：2010-08-15 00:00:00
abstract：:Cell transplantation offers a potential new treatment for stroke. Animal studies using models that produce ischemic damage in both the striatum and the frontal cortex have shown beneficial effects when hNT cells (postmitotic immature neurons) were transplanted into the ischemic striatum. In this study, we investigated...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20800
更新日期：2006-05-01 00:00:00
abstract：:Data from a study in which 12 chronic mental patients and 12 control subject were given D-[3-14C] glucose intravenously in our arterio-venous technique for determining cerebral metabolism in vivo were reexamined. Previously unpublished whole-blood lactate determinations in these experiments indicated a cerebral produc...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490060209
更新日期：1981-01-01 00:00:00
abstract：:Previously, we reported that chelation of intracellular zinc with N, N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine (TPEN)-induced macromolecule synthesis-dependent apoptosis of cultured cortical neurons. According to the current theory of apoptosis, release of mitochondrial cytochrome C into the cytosol is required...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/1097-4547(20000901)61:5<508::AID-JNR5>3.0.
更新日期：2000-09-01 00:00:00
abstract：:In searching for possible differences in the composition of acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) forms in dystrophic brain, the distribution of various enzyme molecules in normal (NB) and dystrophic (DB) 129B6F1/J mouse brain has been investigated. The tissue was sequentially extracted with sa...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19960115)43:2<224::AID-JNR
更新日期：1996-01-15 00:00:00
abstract：:Individuals who regularly shift their sleep timing, like night and/or shift-workers suffer from circadian desynchrony and are at risk of developing cardiometabolic diseases and cancer. Also, shift-work is are suggested to be a risk factor for the development of mood disorders such as the burn out syndrome, anxiety, an...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.24741
更新日期：2021-02-01 00:00:00
abstract：:Sky (also known as "Tyro3" and "Rse") is a member of the Axl/Sky/Mer receptor tyrosine kinase family and has two immunoglobulin-like repeats and two fibronectin type III-like repeats in the extracellular domain. Gas6 is a ligand for all members of the Axl family, each of which (Axl, Sky, and c-Mer) has different affin...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10211
更新日期：2002-04-15 00:00:00
abstract：:Accumulating evidence indicates an immunosuppressive role for CD4(+)CD25(+) regulatory T cells (Tregs) in autoimmune diseases. Although an impaired Treg function in patients with relapsing-remitting multiple sclerosis (RR-MS) has been reported recently, no information is available so far about Treg function in the pro...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20852
更新日期：2006-06-01 00:00:00
abstract：:Although both cytokines and chemokines have been implicated in the pathogenesis of clinical and histological EAE, their interactions in vivo have not yet been clearly established. To address this issue, we evaluated expression of chemokines and receptors in the CNS of wild-type control and cytokine deficient mice at t...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10156
更新日期：2002-03-01 00:00:00
abstract：:Only a few proteins are known to be exclusively expressed in central nervous system (CNS) myelin. A novel surface membrane protein expressed only in CNS myelin and oligodendrocytes of higher vertebrates has been identified by a monoclonal antibody. This CNS myelin/oligodendrocyte-specific protein, MOSP, has a molecula...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490280418
更新日期：1991-04-01 00:00:00
abstract：:Insulin-like growth factor II (IGF-II) plays significant roles in the growth and development of mammals through the regulation of mitogenesis and cell survival. Previously, IGF-II mRNA transcripts within the CNS were detected in the choroid plexus and leptomeninges (DeChiara et al., 1991). The objective of this study ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19971215)50:6<958::AID-JNR
更新日期：1997-12-15 00:00:00
abstract：:Activation of proteolytic enzymes, including the caspase family of proteinases, is a feature characteristic of the apoptotic program. In the present study, we examined a potential role of intracellular proteinases in the death of neuronal PC12 and primary cultured rat microglial cells induced by 6-hydroxydopamine (6-O...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19981015)54:2<214::AID-JNR
更新日期：1998-10-15 00:00:00
abstract：:Glial fibrillary acidic protein (GFAP) is the major constituent of glial filaments and is restricted within the CNS to astrocytes. As with other classes of intermediate filament proteins, the regulation of GFAP expression is poorly understood. Utilizing highly purified cultures of astrocytes and a chemically defined (...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490140202
更新日期：1985-01-01 00:00:00
abstract：:Cultured rat mesencephalic neurons were used to assess the effects of gamma-aminobutyric acid (GABA) transport blockers on toxicity caused by malonate, a reversible, competitive inhibitor of succinate dehydrogenase. Previous studies utilizing an ex vivo chick retinal preparation have shown that GABA release and cell s...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.1052
更新日期：2001-04-01 00:00:00
