Abstract：
:Laminin mediates neural adhesion and process formation. A possible signal transduction pathway for laminin was investigated in both NG108-15 and PC12 neuronal cells using radiolabeling studies as well as various stimulators and inhibitors of phosphatases and kinases. Using [32P]-ortho-phosphate, laminin caused a decrease in the TCA-precipitable counts. Further, laminin stimulated dephosphorylation of laminin binding proteins of 110 kDa, 67 kDa, and 45 kDa and this dephosphorylation was blocked by the phosphatase inhibitor, okadaic acid, and the protein kinase C stimulator, TPA. The phosphatase inhibitors okadaic acid and vanadate, as well as the protein kinase C stimulators, TPA and DAG, blocked laminin-mediated process formation. Inhibitors of kinase activity such as H-7, H-8, and H-9 increased laminin-mediated neural process formation. Since phosphate incorporation into laminin-binding proteins is decreased by laminin and because both phosphatase inhibitors and kinase stimulators inhibit laminin-mediated process formation, we conclude that dephosphorylation events promote the neural cell response to laminin.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Weeks BS,DiSalvo J,Kleinman HKdoi
10.1002/jnr.490270321subject
Has Abstractpub_date
1990-11-01 00:00:00pages
418-26issue
3eissn
0360-4012issn
1097-4547journal_volume
27pub_type
杂志文章abstract：:Tungstate treatment increases the phosphorylation of glycogen synthase kinase-3beta (GSK3beta) at serine 9, which triggers its inactivation both in cultured neural cells and in vivo. GSK3 phosphorylation is dependent on the activation of extracellular signal-regulated kinases 1/2 (ERK1/2) induced by tungstate. As a co...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20726
更新日期：2006-02-01 00:00:00
abstract：:Perceptually similar stimuli, despite not being consciously distinguishable, may result in distinct cortical brain activations. Hypothesizing that perceptually similar tastes are discriminable by electroencephalography (EEG), we recorded 22 human participants' response to equally intense sweet-tasting stimuli: caloric...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.24281
更新日期：2019-03-01 00:00:00
abstract：:Studies using Aplysia californica have demonstrated that transcription after nerve injury occurs during a rapid, transient first phase and a delayed, prolonged second phase. Although the second phase is especially important for regeneration, the mRNAs produced during this phase have not been identified. We characteriz...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20645
更新日期：2005-11-15 00:00:00
abstract：:Four brain-specific, DNase I hypersensitive sites (HSS) have been mapped to the 5' flanking region of the mouse 68-kDa neurofilament gene. These sites are contained within a 1.7-kb sequence that confers neuronal specificity of expression of this gene in transgenic mice. To identify DNA sequences that might be involved...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490320204
更新日期：1992-06-01 00:00:00
abstract：:Nicotinates, pantothenates, riboflavin, vitamins B6 and B12' free (acetyl) and total (free and bound) choline, biopterin, thiamin, biotin, methylated and nonmethylated folates in frontal, temporal, precentral, postcentral, and occipital cortex, thalamus, cerebellum, pons, basal ganglia, and substantia nigra were estim...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490110409
更新日期：1984-01-01 00:00:00
abstract：:Mannitol, which is a cell-impermeable and nontoxic polyalcohol, has been shown to be a useful tool for reversible opening of the blood-brain barrier (BBB). Despite successful clinical trials, the molecular mechanism of the mannitol-induced changes in cerebral endothelial cells (CECs) are poorly understood. For our exp...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20521
更新日期：2005-06-15 00:00:00
abstract：:Photopic L-type responses were recorded from the soma and the axon terminal of horizontal cells of the catfish (Ictalurus punctatus) retina in eye-cup preparations. The responses were produced by a spot of light with 100-micron diameter (intensity, 10 microW/cm2), which was flashed or steadily illuminated and swept al...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490110405
更新日期：1984-01-01 00:00:00
abstract：:Clusterin (apolipoprotein J) is a highly conserved, multifunctional, vertebrate glycoprotein. Several isoforms of clusterin have been described including the predominant secreted isoform (sCLU) and several nuclear isoforms (nCLU) associated with cell death. sCLU has been shown to bind a variety of partly unfolded, str...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20374
更新日期：2005-02-15 00:00:00
abstract：:The occurrence of type-1 plasminogen activator inhibitor (PAI-1) in human cerebrospinal fluid (CSF) has not previously been reported. As a member of the serpin superfamily of serine protease inhibitors and an acute phase response component, PAI-1 has powerful potential roles in nervous system homeostasis. We have dete...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490340311
更新日期：1993-02-15 00:00:00
abstract：:CD200, a type I transmembrane glycoprotein, can interact with its receptor CD200R, which plays an inhibitory role in the activation of microglia-the resident macrophages of the central nervous system. In this study, the rat C6 glioma cell line (C6-1) that was previously characterized with high in vivo tumorigenicity w...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23922
更新日期：2016-12-01 00:00:00
abstract：:Two clones encoding human glial fibrillary acidic protein (GFAP) were isolated from a human astrocytoma cDNA library. The clones pHGFAP1 and pHGFAP2 were selected by the combined use of differential colony hybridization and hybridization-selection technique with polyclonal anti GFAP antiserum. The longer one, pHGFAP1,...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490200204
更新日期：1988-01-01 00:00:00
abstract：:A new subdivision with distinctive morphological and functional attributes has been identified at the caudomedial margin of the neostriatum and surrounding the rostrolateral border of the globus pallidus in the brains of the rat, cat, monkey, and human. The subdivision is termed marginal division (MrD) based on its lo...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：
更新日期：1999-10-15 00:00:00
abstract：:The cellular localization of calpain is important in understanding the roles that calpain may play in physiological function. We, therefore, examined calpain expression, activity, and immunofluorescent localization in primary cultures of rat oligodendrocytes. The mRNA expression of m-calpain was 64.8% (P = 0.0033) and...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10414
更新日期：2002-11-15 00:00:00
abstract：:Acrylamide (ACR) is an environmental toxicant and prototypic tool for studying mechanisms of peripheral neuropathies. Reductions in fast anterograde axonal transport (faAXT) are thought to be a critical step leading to axonal degeneration. Kinesin and microtubules (MT) were evaluated as molecular sites of action using...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19961001)46:1<7::AID-JNR2>
更新日期：1996-10-01 00:00:00
abstract：:There are a considerable number of studies concerning the behavioral effects of physical exercise on the epileptic brain; however, the intracellular signaling mechanisms involved remain unclear. We investigated the effects of aerobic exercise on hippocampal levels of brain-derived neurotrophic factor (BDNF), expressio...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.24196
更新日期：2018-05-01 00:00:00
abstract：:The relationship between brain myelination and nutritional insufficiency in suckling rats whose dams had a restricted dietary intake was studied. The undernourished pups were characterized by body weight and brain weight that were 40-70% and 82-88% that of controls throughout the suckling period. Myelin concentrations...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490190311
更新日期：1988-03-01 00:00:00
abstract：:The influence of a concentrated extract of soluble substances from the sciatic nerve upon the acetylcholine receptor (AChR) content in the soleus muscle of adult rats was examined by in vivo infusions. Internal and membrane-inserted AChR were quantitated by the specific binding of 125I-alpha-bungarotoxin (a-BuTX). Int...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490160307
更新日期：1986-01-01 00:00:00
abstract：:Understanding the pathway for amyloid percursor protein (APP) catabolism has become an important line of investigation. APP is a ubiquitous membrane bound protein that is rapidly cleaved at the membrane, yielding a secreted protein identical to protease nexin II and an internalized 11.5 kDa 100 residue C terminal deri...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490330121
更新日期：1992-09-01 00:00:00
abstract：:Recent advances in human induced pluripotent stem cells (hiPSCs) offer new possibilities for biomedical research and clinical applications. Differentiated neurons from hiPSCs are expected to be useful for developing novel methods of treatment for various neurological diseases. However, the detailed process of function...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23666
更新日期：2015-12-01 00:00:00
abstract：:Post-translational modification of neural cell adhesion molecule (NCAM) with alpha2,8-linked polysialic acid, which regulates homophilic adhesion and/or signal transduction events, is crucial to synaptic plasticity in the developing and adult brain. Evidence from in vitro models has implicated polysialylation in the r...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/1097-4547(20000915)61:6<636::AID-JNR7>3.0.
更新日期：2000-09-15 00:00:00
abstract：:The present study examined the effects of high-altitude exposure on the pineal gland, the main source of production of melatonin. It was surmised that hypoxia experienced at high altitude, caused by decreased oxygen tension in the ambient air, might lead to some structural alterations in the pineal gland and, hence, a...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10136
更新日期：2002-02-15 00:00:00
abstract：:The BM89 antigen, first identified in porcine brain by means of a monoclonal antibody, is a neuron-specific molecule widely distributed in the mammalian central and peripheral nervous system (Merkouri and Matsas: Neuroscience 50:53-68, 1992). Here we describe the purification of BM89 antigen from porcine and mouse bra...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：
更新日期：1997-06-15 00:00:00
abstract：:The changes in ganglioside composition and metabolism of deafferentiated rat hippocampus were estimated after septal lesion. A significant decrease in total ganglioside concentration was found 7 days after the lesion. The reduced level of total gangliosides persisted at 17 and 25 days. Relative increase in the proport...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490210107
更新日期：1988-09-01 00:00:00
abstract：:Axotomy of peripheral nerves in neonatal rats induces motoneuron death that can be delayed but not arrested by the application of several neurotrophic factors (NFs) or adenoviral vectors carrying genes for NFs. We tested whether an adenoviral vector carrying the gene for glial cell-line-derived neurotrophic factor (Ad...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19981215)54:6<766::AID-JNR
更新日期：1998-12-15 00:00:00
abstract：:The location of glyoxylate-complex molecules has been investigated in several areas of the rat cerebral cortex using the immunohistochemical peroxidase-antiperoxidase (PAP) method. Antibodies against glyoxylate-complex molecules have been developed in the rabbit after immunization with a glyoxylate-bovine serum albumi...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19980115)51:2<268::AID-JNR
更新日期：1998-01-15 00:00:00
abstract：:Utilizing a specific polyclonal antibody against a peptide unique for brain-derived neurotrophic factor (BDNF), we investigated the regional and temporal profiles of immunoreactivity of the BDNF protein in the rat hippocampus after transient forebrain ischemia. The pattern of immunoreactivity for the BDNF receptor (Tr...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19980801)53:3<318::AID-JNR
更新日期：1998-08-01 00:00:00
abstract：:Gap junctions (GJs) are widely distributed in brains across the animal kingdom. To visualize the GJ- coupled networks of two major mechanosensory neurons in the ganglia of medicinal leeches, we injected these cells with the GJ-permeable tracer Neurobiotin. When diffusion time was limited to only 30 min, tracer couplin...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.24599
更新日期：2020-06-01 00:00:00
abstract：:We investigated whether a learning impairment after a controlled cortical impact (CCI) injury was associated with alterations in molecules involved in synaptic plasticity and learning and memory. Adult male rats with moderate CCI to the left parietal cortex, tested in a Morris water maze (MWM) beginning at postinjury ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21893
更新日期：2009-02-15 00:00:00
abstract：:Ionotropic N-methyl-D-aspartate (NMDA) receptor agonists, L-aspartic acid (L-Asp) and NMDA, have been shown to inhibit histamine-stimulated acid secretion, but their effect on gastric mucosal blood flow (GMBF) is largely unknown. The aim of this study was to investigate whether L-Asp and NMDA inhibit histamine-stimula...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20202
更新日期：2004-09-01 00:00:00
abstract：:Previous studies have shown that PC12 cells overexpressing beta/A4 amyloid peptide display altered morphology characterized by pronounced membrane ruffling and extensive intercellular appositions. Having observed other cell types in which these features accompany increased connexin43 (Cx43) production and gap junction...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19960415)44:2<124::AID-JNR
更新日期：1996-04-15 00:00:00
