Abstract:
:Mannitol, which is a cell-impermeable and nontoxic polyalcohol, has been shown to be a useful tool for reversible opening of the blood-brain barrier (BBB). Despite successful clinical trials, the molecular mechanism of the mannitol-induced changes in cerebral endothelial cells (CECs) are poorly understood. For our experiments, we used CECs in culture, which were treated with different, clinically relevant concentrations of mannitol. We found that mannitol induced a rapid, concentration-dependent, and reversible tyrosine phosphorylation of a broad range of proteins between 50 and 190 kDa. One of the targets of tyrosine phosphorylation turned out to be the adherens junction protein beta-catenin. Phosphorylation of beta-catenin on tyrosine residues caused its subcellular redistribution and its dissociation from cadherin and alpha-catenin as shown by coimmunoprecipitation studies. All these effects could be inhibited by the Src kinase inhibitor PP-1 but not by the Erk inhibitor U0126, the Rho kinase inhibitor Y27632, or the calcium channel blocker verapamil. Because beta-catenin is a key component of the junctional complex, its Src-mediated phpsphorylation may play an important role in the mannitol induced reversible opening of the BBB.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Farkas A,Szatmári E,Orbók A,Wilhelm I,Wejksza K,Nagyoszi P,Hutamekalin P,Bauer H,Bauer HC,Traweger A,Krizbai IAdoi
10.1002/jnr.20521keywords:
subject
Has Abstractpub_date
2005-06-15 00:00:00pages
855-61issue
6eissn
0360-4012issn
1097-4547journal_volume
80pub_type
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