Abstract:
:To determine whether one or more opioid receptor types might be preferentially involved in gliogenesis, primary mixed glial cultures derived from mouse cerebra were continuously treated with varying concentrations of opioid agonists selective for mu (mu), i.e., DAGO ([D-Ala2, MePhe4, Gly(ol)5]enkephalin), delta (delta), i.e., DPDPE ([D-PEN2,D-PEN5]enkephalin), or kappa (kappa), i.e., U69,593, opioid receptor types. In addition, a group of cultures was treated with [Met5]-enkephalin, an agonist for delta opioid receptors as well as putative zeta (zeta) opioid receptors. Opioid-dependent changes in growth were assessed by examining alterations in (1) the number of cells in mixed glial cultures at 3, 6, and 8 days in vitro (DIV), (2) [3H]thymidine incorporation by glial fibrillary acidic protein (GFAP) immunoreactive, flat (type 1) astrocytes at 6 DIV, and (3) the area and form factor of GFAP-immunoreactive, flat (type 1) astrocytes. DPDPE at 10(-8) or 10(-10) M, as well as [Met5]-enkephalin at 10(-6), 10(-8), or 10(-10) M, significantly reduced the total number of glial cells in culture; but this effect was not observed with DAGO or U69,593 (both at 10(-6), 10(-8), or 10(-10) M). Equimolar concentrations (i.e., 10(-6) M) of [Met5]enkephalin or U69,593, but not DPDPE or DAGO, suppressed the rate of [3H]thymidine incorporation by GFAP-immunoreactive, flat (type 1) astrocytes. DAGO had no effect on growth, although in previous studies morphine was found to inhibit glial numbers and astrocyte DNA synthesis. [Met5]enkephalin (10(-6) M) was the only agonist to significantly influence astrocyte area. Collectively, these results indicate that delta (and perhaps mu) opioid receptor agonists reduce the total number of cells in mixed glial cultures; while [Met5]enkephalin-responsive (and perhaps kappa-responsive) opioid receptors mediate DNA synthesis in astrocytes. This implies that delta opioid receptors, as well as [Met5]enkephalin-sensitive, non-delta opioid receptors, mediate opioid-dependent regulation of astrocyte and astrocyte progenitor growth. These data support the concept that opioid-dependent changes in central nervous system growth are the result of endogenous opioid peptides acting through multiple opioid receptor types.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Stiene-Martin A,Hauser KFdoi
10.1002/jnr.490290415subject
Has Abstractpub_date
1991-08-01 00:00:00pages
538-48issue
4eissn
0360-4012issn
1097-4547journal_volume
29pub_type
杂志文章abstract::L-deprenyl protects neurons in a number of in vivo and in vitro models and it has been postulated that it ameliorates some neurodegenerative disorders. Superoxide dismutase (SOD) is one of the enzymes responsible for the inactivation of oxygen free radicals, and one of the mechanisms of the effect of L-deprenyl is tho...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19980715)53:2<235::AID-JNR
更新日期:1998-07-15 00:00:00
abstract::There are a considerable number of studies concerning the behavioral effects of physical exercise on the epileptic brain; however, the intracellular signaling mechanisms involved remain unclear. We investigated the effects of aerobic exercise on hippocampal levels of brain-derived neurotrophic factor (BDNF), expressio...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.24196
更新日期:2018-05-01 00:00:00
abstract::Cytoskeletal structures under the cell membrane carry out pivotal roles in the maintenance and remodeling of the cell structures. Reforming of the cytoskeletal networks after partial extraction of membrane components could be a good clue to identify molecular components pertaining the interaction of cytoskeleton with ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20555
更新日期:2005-07-15 00:00:00
abstract::Myelin-associated glycoprotein (MAG) is a potent inhibitor of axonal regeneration and also, depending on the age and type of neuron, can promote axonal growth. In addition, MAG influences stability of both myelin and the axon in the intact, mature nervous system. The identity of the neuron/axonal MAG-binding receptor ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(sici)1097-4547(19990415)56:2<213::aid-jnr
更新日期:1999-04-15 00:00:00
abstract::Although the thalamus is not considered primarily as a limbic structure, abundant evidence indicates the essential role of the thalamus as a modulator of limbic functions indirectly through the amygdala. The amygdala is a central component of the limbic system and serves an essential role in modulating the core proces...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.24235
更新日期:2018-07-01 00:00:00
abstract::Rho-GTPase has been implicated in the apoptosis of many cell types, including neurons, but the mechanism by which it acts is not fully understood. Here, we investigate the roles of Rho and ROCK in apoptosis during transplantation of embryonic stem cell-derived neural precursor cells. We find that dissociation of neura...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21502
更新日期:2008-02-01 00:00:00
abstract::Apolipoprotein E (apo-E), a protein involved in lipid metabolism and cholesterol transport, has been found to be up-regulated in CNS injury and is associated with Alzheimer's disease in humans. In this study, we show that apo-E is also up-regulated after complete spinal cord transection in the C57BL/6 mouse. In the un...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10482
更新日期:2003-02-01 00:00:00
abstract::The concentration of myelin basic protein (MBP) mRNA in primary cultures of cells dissociated from embryonic mouse cerebra and grown in the presence of varying amounts of thyroid hormone was measured using a 32P-labeled cDNA probe and a dot-blot procedure. The cDNA probe contained 1.85 kilobases of the gene for MBP. T...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490170304
更新日期:1987-01-01 00:00:00
abstract::Apoptosis and neuronal atrophy are commonly seen in patients infected with the human immunodeficiency virus type 1 (HIV-1) in the late phase of infection. The HIV-1 envelope glycoprotein gp120 has been suggested to be a causal agent of neuronal loss. Therefore, blocking gp120 neurotoxicity may be an effective way to r...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20780
更新日期:2006-04-01 00:00:00
abstract::Astrocytes are glial cells that are distributed throughout the central nervous system in an arrangement optimal for chemical and physical interaction with neuronal synapses and brain blood supply vessels. Neurotransmission modulates astrocytic excitability by activating an array of cell surface receptors and transport...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24075
更新日期:2017-12-01 00:00:00
abstract::Neuropathic pain is a debilitating chronic pain and represents a major clinical challenge. The molecular and cellular mechanisms underlying its development and maintenance are not fully understood but involve abnormal excitability in the dorsal horn of the spinal cord. A growing body of evidence has shown that this ab...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.23816
更新日期:2017-06-01 00:00:00
abstract::Neurotrophins may have a neuroprotective role and are probably involved in the control of axonal sprouting and synaptic plasticity. An antibody raised against a pro-sequence of nerve growth factor (NGF) was tested. In control undisturbed rats, a strong immunoreactivity was detected in scattered cells in and around the...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490350409
更新日期:1993-07-01 00:00:00
abstract::Thymocyte differentiation antigen-1 (Thy-1) is a cell surface glycoprotein found on T cells and neurons and is involved in cell-to-cell interactions. In addition, Thy-1 knockouts (KO) are a potential mouse model of restless legs syndrome (RLS) based on clinical observations and the role of dopamine in the disease. In ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23046
更新日期:2012-08-01 00:00:00
abstract::Culturing sympathetic ganglion neurons in vitro may modify phenotypic expression of some neurotransmitters. For dorsal root ganglia (DRG), contradictory results have been reported; most studies have used immature material. We have therefore performed a detailed immunocytochemical analysis of the transmitter content of...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490220414
更新日期:1989-04-01 00:00:00
abstract::Neuronal progenitors in the adult hippocampus continually proliferate and differentiate to the neuronal lineage, and ischemic insult promotes hippocampal neurogenesis. However, newborn neurons show a progressive reduction in numbers during the initial few weeks, therefore, enhanced survival of newborn neurons seems to...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21036
更新日期:2006-11-01 00:00:00
abstract::Cisplatin is a highly effective chemotherapeutic agent but with significant ototoxic side effects. Apoptosis is an important mechanism of cochlear hair cell loss following exposure to an ototoxic level of cisplatin. The present study investigated the effects of the cannabinoid receptor 2 (CB2) ligand JWH-015 on cispla...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21168
更新日期:2007-03-01 00:00:00
abstract::In cultured Schwann cells, single-channel and whole-cell K+ currents can be activated by depolarizing the membrane to values more negative than -50 mV. In elevated extracellular K+ concentration ([K+]o), however, single-channel activity and whole-cell currents could be recorded at more negative potentials. Thus, the t...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490280208
更新日期:1991-02-01 00:00:00
abstract::We have previously shown in rat primary cultured mesencephalic cells that cyanide induces a high level of oxidative stress and necrotic death. To evaluate the mechanism of the cytotoxicity, the effects of cyanide on intracellular glutathione (GSH) pools and inducible nitric oxide synthase (iNOS)-mediated reactive nitr...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20998
更新日期:2006-10-01 00:00:00
abstract::Although reactive oxygen species (ROS) have been implicated in ischemic preconditioning (IPC)-induced neuronal protection, several key questions concerning ROS remain to be elucidated. The purpose of this study is to obtain direct evidence for the formation of specific ROS species generated by IPC, and to determine th...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20402
更新日期:2005-03-15 00:00:00
abstract::Heat shock proteins (HSPs) are stress-responsive proteins that serve as important molecules contributing to cellular "protein triage." We and others have reported an increase of selected HSPs in multiple sclerosis (MS) lesions. However, the exact expression pattern of HSP family genes in MS is not known. The aim of ou...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23079
更新日期:2012-10-01 00:00:00
abstract::The medial prefrontal cortex (mPFC) is critical for selecting and shaping complex behavioral responses. In rodent models of neuropathic pain there is evidence for both structural and functional changes in the mPFC. Brain derived neurotrophic factor (BDNF) plays a critical role in the normal functioning of the mPFC. It...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.24095
更新日期:2017-12-01 00:00:00
abstract::The cholinergic system takes part in the immune-neuroendocrine integration. Lymphocytes have been found to express muscarinic and nicotinic cholinergic receptors, as well as acetylcholine-esterase on their surface, and cholinergic agents modulate immune functions in vitro and in vivo. In the present study we provide e...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490350209
更新日期:1993-06-01 00:00:00
abstract::Pigment epithelium-derived factor (PEDF) is a neurotrophic protein synthesized and secreted by retinal pigment epithelial (RPE) cells in early embryogenesis and has been shown to be present in the extracellular matrix between the RPE cells and the neural retina. It induces neuronal differentiation and promotes surviva...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1999-09-15 00:00:00
abstract::The location of glyoxylate-complex molecules has been investigated in several areas of the rat cerebral cortex using the immunohistochemical peroxidase-antiperoxidase (PAP) method. Antibodies against glyoxylate-complex molecules have been developed in the rabbit after immunization with a glyoxylate-bovine serum albumi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19980115)51:2<268::AID-JNR
更新日期:1998-01-15 00:00:00
abstract::The cellular localization of calpain is important in understanding the roles that calpain may play in physiological function. We, therefore, examined calpain expression, activity, and immunofluorescent localization in primary cultures of rat oligodendrocytes. The mRNA expression of m-calpain was 64.8% (P = 0.0033) and...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10414
更新日期:2002-11-15 00:00:00
abstract::Previous histochemical studies of iron in the brain demonstrated iron enrichment in oligodendrocytes, but the iron-enriched oligodendrocytes had a spatially restricted distribution that excluded major white matter tracts. In this study we used techniques designed to permeabilize tissue, combined with iron histochemist...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490260415
更新日期:1990-08-01 00:00:00
abstract::The effects of glutamate on intracellular free Ca2+, [Ca2+]i, and neurotoxicity were compared in cerebellar granule neurons in vitro. [Ca2+]i was measured with fura-2 and digital fluorescence imaging microscopy; neurotoxicity was monitored using a vital dye and colorimetric analysis. Glutamate produced dose-dependent ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490280317
更新日期:1991-03-01 00:00:00
abstract::We used intracellular recording to investigate the functional interaction between protein kinase C (PKC) and protein kinase A (PKA) signal transduction cascades in the control of transmitter release in the neuromuscular synapses from adult rats. Our results indicate that: 1) PKA and PKC are independently involved in a...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21885
更新日期:2009-02-15 00:00:00
abstract::Growing evidence suggests that decreased brain-derived neurotrophic factor (BDNF) levels are associated with Alzheimer's disease (AD) pathogenesis. Therefore, BDNF gene therapy is considered to be a promising therapeutic strategy for treating AD. Sendai virus (SeV) is a type I parainfluenza virus that does not interac...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22830
更新日期:2012-05-01 00:00:00
abstract::We studied the postischemic alterations of second messenger and receptor systems focusing on the strionigral pathway in order to clarify the mechanism of the delayed neuronal changes in remote areas of the rat brain after transient focal ischemia. Chronological changes of [3H]forskolin and [3H]SCH 23390 binding sites ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490330315
更新日期:1992-11-01 00:00:00