Abstract:
:Cisplatin is a highly effective chemotherapeutic agent but with significant ototoxic side effects. Apoptosis is an important mechanism of cochlear hair cell loss following exposure to an ototoxic level of cisplatin. The present study investigated the effects of the cannabinoid receptor 2 (CB2) ligand JWH-015 on cisplatin-induced apoptosis. CB2 mRNA was constitutively expressed in the auditory cell line HEI-OC1. By using MTT assay, DNA fragmentation, and FACS analysis, we demonstrated that apoptosis induced by cisplatin was inhibited by treatment with JWH-015 in a dose-dependent manner. Activation of caspase-3, caspase-8, and caspase-9 was detected after treatment with cisplatin, and the cleavage of poly-(ADP)-ribose polymerase (PARP) was observed within cisplatin-treated HEI-OC1 cells. JWH-015 inhibited the activation of caspase-3, caspase-8, and caspase-9; cleavage of PARP; and release of cytochrome c. JWH-015 also inhibited the apoptosis through activation of the extracellular signal-regulated kinase pathway. Finally, JWH-015 inhibited cisplatin-induced reactive oxygen species and tumor necrosis factor-alpha production. Collectively, these findings show that blocking a critical step in apoptosis by using JWH-015 may be a useful strategy to prevent harmful side effects of cisplatin ototoxicity in patients having to undergo chemotherapy.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Jeong HJ,Kim SJ,Moon PD,Kim NH,Kim JS,Park RK,Kim MS,Park BR,Jeong S,Um JY,Kim HM,Hong SHdoi
10.1002/jnr.21168subject
Has Abstractpub_date
2007-03-01 00:00:00pages
896-905issue
4eissn
0360-4012issn
1097-4547journal_volume
85pub_type
杂志文章abstract::Multiple sclerosis (MS) is an inflammatory, demyelinating disease of the central nervous system with an autoimmune attack on the components of the myelin sheath and axons. The etiology of the disease remains largely unknown, but it is commonly acknowledged that the development of MS probably results from the interacti...
journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
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journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
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doi:10.1002/jnr.22003
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490250407
更新日期:1990-04-01 00:00:00
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journal_title:Journal of neuroscience research
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doi:10.1002/jnr.490290417
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journal_title:Journal of neuroscience research
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doi:10.1002/jnr.10269
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abstract::Butyrate produced a biphasic modulation of the thyroid hormone receptor in neuroblastoma N2A cells increasing receptor number by 20-35% at concentrations 0.25-0.75 mM and decreasing receptor levels by 30-55% at 2-4 mM. The half-life of the receptor, as assessed by its disappearance after incubation with 18 microM cycl...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490270102
更新日期:1990-09-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23847
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abstract::Amyloid beta-peptide (A beta) likely causes functional alterations in neurons well prior to their death. Nuclear factor-kappaB (NF-kappaB), a transcription factor that is known to play important roles in cell survival and apoptosis, has been shown to be modulated by A beta in neurons and glia, but the mechanism is unk...
journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490400409
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(20000215)59:4<513::AID-JNR
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.24193
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19981215)54:6<766::AID-JNR
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
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journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490320108
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10832
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abstract::Neural stem cells (NSCs) have great potential as a therapeutic tool for the repair of a number of CNS disorders. NSCs can either be isolated from embryonic and adult brain tissue or be induced from both mouse and human ES cells. These cells proliferate in vitro through many passages without losing their multipotential...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
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更新日期:2002-06-01 00:00:00
abstract::Pathogenesis of Parkinson's disease and related catecholaminergic neurological disorders is closely associated with changes in the levels of tyrosine hydroxylase (TH). Therefore, investigation of the regulation of the TH gene system should assist in understanding the pathomechanisms involved in these neurological diso...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23085
更新日期:2012-10-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21893
更新日期:2009-02-15 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490050308
更新日期:1980-01-01 00:00:00
abstract::Neuronal pathology of the brain with Alzheimer's disease (AD) is characterized by numerous depositions of amyloid-beta peptides (Abeta). Abeta binding to the 75-kDa neurotrophin receptor (p75NTR) causes neuronal cell death. Here we report that Abeta causes cell death in neuronal hybrid cells transfected with p75NTR, b...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10703
更新日期:2003-09-01 00:00:00
abstract::Haptoglobin (Hp), an acute-phase response protein, is typically increased in the serum of adults after acute tissue injury. It is an antioxidant and may function as an injury-induced neuroprotective protein. However, the source of increased Hp is not clear. To investigate its source, we compared its time course expres...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23159
更新日期:2013-02-01 00:00:00