Abstract：
:Experimental allergic encephalomyelitis (EAE), an animal model for multiple sclerosis (MS), is a demyelinating autoimmune disease of the central nervous system (CNS). The proinflammatory cytokine TNF alpha, as an endogenous mediator of inflammation, plays an important role in the pathogenesis of EAE disease. In this study, we demonstrate the presence of TNF alpha in spinal cord of Lewis rats, during the critical phase of EAE. The expression of TNF alpha is observed mainly in the gray matter of thoracic and lumbar levels of the spinal cord, in the motoneurons and interneurons of the ventral horn. Surprisingly, one month after recovery, we still found an intense TNF alpha-neuronal expression, including in the cervical region, and this positivity lasted up to 40 days after recovery, with, however, a decrease in its intensity. These results suggest that central neurons respond directly to massive infiltration of lymphocytes and macrophages after the breakdown of the blood-brain barrier (BBB), by producing TNF alpha cytokine. In addition, neuronal-TNF alpha detection in the recovery stage of EAE may suggest a role other than its classical action in promoting inflammatory processes.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Villarroya H,Marie Y,Ouallet JC,Le Saux F,Tchélingérian JL,Baumann Ndoi
10.1002/(SICI)1097-4547(19970901)49:5<592::AID-JNRsubject
Has Abstractpub_date
1997-09-01 00:00:00pages
592-9issue
5eissn
0360-4012issn
1097-4547pii
10.1002/(SICI)1097-4547(19970901)49:5<592::AID-JNRjournal_volume
49pub_type
杂志文章abstract：:The cellular localization of calpain is important in understanding the roles that calpain may play in physiological function. We, therefore, examined calpain expression, activity, and immunofluorescent localization in primary cultures of rat oligodendrocytes. The mRNA expression of m-calpain was 64.8% (P = 0.0033) and...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10414
更新日期：2002-11-15 00:00:00
abstract：:Rho-GTPase has been implicated in the apoptosis of many cell types, including neurons, but the mechanism by which it acts is not fully understood. Here, we investigate the roles of Rho and ROCK in apoptosis during transplantation of embryonic stem cell-derived neural precursor cells. We find that dissociation of neura...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21502
更新日期：2008-02-01 00:00:00
abstract：:Primary fibroblasts initiated from skin biopsies of Wistar rats were transfected with a plasmid that encodes the human growth hormone and the neomycin resistance genes. Cell clones selected for G418 resistance and expressing high levels of human growth hormone were propagated in vitro and subsequently transplanted int...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490290304
更新日期：1991-07-01 00:00:00
abstract：:PC12 cells can differentiate into neuron-like cells after treatment with either nerve growth factor (NGF) or transduction with a retrovirus which expresses the K-ras oncogene. The concomitant treatment of NGF plus ras differentiates PC12 cells further than either agent alone with respect to neurite outgrowth, acetylch...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490420306
更新日期：1995-10-15 00:00:00
abstract：:Evidence indicates that the ubiquitin-proteasome system and the endoplasmic retculum (ER) quality-control system work in concert to ensure that proteins are correctly folded in the ER and that misfolded proteins are retrotransported to the cytosol for degradation by proteasomes. Dysfunction of either system results in...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23413
更新日期：2014-10-01 00:00:00
abstract：:Neural precursor cells, including neural stem and progenitor cells, in the subventricular zone (SVZ) are the main targets for cytomegalovirus (CMV) infection in developing brains. The neural precursor cells in the SVZ of the adult brain have been reported to respond by proliferating after infusion with epidermal growt...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21410
更新日期：2007-10-01 00:00:00
abstract：:The role of axons in the expression of the major myelin glycoprotein, P0, has been investigated using neuron/Schwann cell cultures. These cultures were either nonmyelinating or myelinating due to growth in defined medium or in medium containing serum and chick embryo extract, respectively. The neurons and Schwann cell...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490260202
更新日期：1990-06-01 00:00:00
abstract：:A rodent model of Parkinson's disease, the 1-methyl-4-(2'-methylphenyl)-1,2,3,6-tetrahydropyridine treated mouse, was used to determine whether striatal dopamine levels recover following grafting adrenal medulla into the striatum. Four types of grafts were performed: 1) adult mouse adrenal medulla, 2) adult adrenal me...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490250303
更新日期：1990-03-01 00:00:00
abstract：:We studied the postischemic alterations of second messenger and receptor systems focusing on the strionigral pathway in order to clarify the mechanism of the delayed neuronal changes in remote areas of the rat brain after transient focal ischemia. Chronological changes of [3H]forskolin and [3H]SCH 23390 binding sites ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490330315
更新日期：1992-11-01 00:00:00
abstract：:Using subtype-specific Ca-channel blockers, we have characterised the voltage-sensitive Ca2+ currents as well as neurotransmitter release from cultured mouse cerebellar granule cells. The whole cell version of the patch clamp technique was adapted to monitor the isolated Ca-channel currents. The currents were activate...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(sici)1097-4547(19970401)48:1<43::aid-jnr4
更新日期：1997-04-01 00:00:00
abstract：:To further our understanding of the functions of the major myelin proteins, myelin basic protein (MBP) and proteolipid protein (PLP), and other myelin proteins, such as 2'3'-cyclic nucleotide 3'-phosphodiesterase (CNP) and myelin-associated glycoprotein (MAG), bovine brain myelin was extracted with Triton X-100, and p...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10383
更新日期：2002-10-01 00:00:00
abstract：:After left unilateral cochlear ablation (UCA) in young adult guinea pigs, the appearance of plasticities in auditory pathways suggested altered gene expression and modified phenotypic behaviors of auditory neurons. Because phosphorylated cyclic-AMP response element-binding protein (CREB-P) is a transcription factor th...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20820
更新日期：2006-05-15 00:00:00
abstract：:Production of hydrogen peroxide as a by-product of the breakdown of catecholamines by the enzyme monoamine oxidase (MAO) has been hypothesized to contribute to the increased proclivity of dopaminergic neurons for oxidative injury. We established clonal dopaminergic PC12 cell lines which have elevated MAO activity leve...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19961215)46:6<666::AID-JNR
更新日期：1996-12-15 00:00:00
abstract：:In culture, cerebellar granule neurons die of apoptosis in serum-free media containing a physiologic level of K(+) but survive in a depolarizing concentration of K(+) or when insulin-like growth factor 1 (IGF-1) is added. Both Akt/PKB activation and caspase-3 inhibition were implicated as the underlying neuroprotectiv...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20024
更新日期：2004-03-15 00:00:00
abstract：:The production of collagen-degrading proteases by cultured neonatal rat microglia was examined using an immobilized fibronectin-gelatin matrix coupled to a fluorescent marker and by substrate gel analysis. When microglia were plated onto the surface of the matrix and incubated under resting (nonstimulated) conditions,...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490350309
更新日期：1993-06-15 00:00:00
abstract：:The gene mutated in the mouse open brain (opb) phenotype antagonizes sonic hedgehog-mediated signaling and encodes a small GTPase of the Rab family, Rab23. To date, the brain expression profile and exact mechanism of function of the Rab23 protein has remained unknown. Specific antibodies generated against Rab23 showed...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20788
更新日期：2006-05-01 00:00:00
abstract：:To explore the feasibility of cell type-specific gene expression in oligodendrocytes as a possible therapeutic approach for demyelinating diseases, the cell specificity, tissue specificity, and duration of gene expression were investigated using recombinant adeno-associated viral vectors (rAAV) carrying a green fluore...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19990215)55:4<504::AID-JNR
更新日期：1999-02-15 00:00:00
abstract：:A morphogenic role of neurotransmitters during cellular differentiation in vitro has been demonstrated in recent years. Using in situ hybridization, we confirm the presence of the D1 receptor at E16 and show additionally that the transcript is relatively widespread and present in both proliferative and differentiating...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19960215)43:4<439::AID-JNR
更新日期：1996-02-15 00:00:00
abstract：:The ErbB family, ErbB1 (also known as the epidermal growth factor receptor EGFR), ErbB2, ErbB3, and ErbB4 comprise a group of receptor tyrosine kinases that interact with ligands from the epidermal growth factor (EGF) superfamily, subsequently dimerize, catalytically activate each other by cross-phosphorylation, and t...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20381
更新日期：2005-03-01 00:00:00
abstract：:Cultured Schwann cells treated with heregulin growth factor require costimulation with a cyclic adenosine monophosphate-elevating agent to produce maximal cell proliferation. Gene chip expression analysis was used to identify genes that are induced or repressed when Schwann cells are treated with heregulin and/or fors...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10679
更新日期：2003-08-15 00:00:00
abstract：:Fourteen-day gestation fetal cerebral cortex homografts were transplanted into the thoracic (T6) spinal cord between the left dorsal column and dorsal horn of adult host rats. The transplants were soaked in 2.0 micrograms/ml of the lectin Phaseolus vulgaris leucoagglutinin (PHAL) prior to implantation. Transplanted ho...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490170410
更新日期：1987-01-01 00:00:00
abstract：:Adhesion responses triggered by integrin-class matrix receptors have been implicated in the synaptic reorganization events necessary for certain types of neuronal plasticity. Hippocampal slice cultures were used to test whether the related structural transformations elicited by NMDA receptor stimulation are regulated ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(20000315)59:6<827::AID-JNR
更新日期：2000-03-15 00:00:00
abstract：:Human P0 is the main myelin glycoprotein of the peripheral nervous system. It can bind six different glycans, all linked to Asn(93) , the unique glycosylation site. Other myelin glycoproteins, also with a single glycosylation site (PMP22 at Asn(36) , MOG at Asn(31) ), bind only one glycan. The MAG has 10 glycosylation...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.23462
更新日期：2015-01-01 00:00:00
abstract：:To promote neurite elongation, nerve growth cones must adhere to other surfaces. A complex of integral membrane glycoproteins mediates cell binding to the extracellular glycoproteins fibronectin and laminin (Horwitz et al., J Cell Biol 101:2134-2144, 1985). The receptor complex, named integrin, binds to fibronectin by...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490210221
更新日期：1988-10-01 00:00:00
abstract：:A sensitive and convenient method of endopeptidase assay using as substrate globin modified with pyridoxal-5-phosphate was used for determination of acid proteinases in bovine hypothalamus separated by isoelectric focusing. The soluble protein fraction of hypothalamus upon elution from Sephadex gave five peaks of prot...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490040505
更新日期：1979-01-01 00:00:00
abstract：:Nerve growth factor (NGF) is a homodimer that binds to two distinct receptor types, TrkA and p75, to support survival and differentiation of neurons. The high-affinity binding on the cell surface is believed to involve a heteroreceptor complex, but its exact nature is unclear. We developed a heterodimer (heteromutein)...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23116
更新日期：2012-12-01 00:00:00
abstract：:Previous animal studies have linked white matter damage to certain schizophrenia-like behaviors in cuprizone (CPZ)-exposed mouse. Mitochondrial dysfunction, oxidative stress, neuroinflammation, and oligodendrocyte loss coexist in the brain of such mice. The aim of this study was to examine effects of the antioxidant N...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.24189
更新日期：2018-05-01 00:00:00
abstract：:Calpain, an intracellular cysteine protease, has been widely reported to be involved in neuronal cell death. The purpose of this study is to investigate the role of calpain activation in axonal damage-induced retinal ganglion cell (RGC) death. Twelve-week-old male calpstatin (an endogenous calpain inhibitor) knockout ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22800
更新日期：2012-04-01 00:00:00
abstract：:FE65 is a key "adapter" protein that links a multiprotein complex to an intracellular domain of beta-amyloid precursor protein (betaPP). Its overexpression modulates the trafficking of betaPP and facilitates the generation of beta-amyloid (Abeta). FE65 is predominantly expressed in brain tissues. An exon 9-inclusive i...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(20000401)60:1<73::AID-JNR8
更新日期：2000-04-01 00:00:00
abstract：:The brain acid-soluble protein BASP1 (CAP-23, NAP-22) belongs to the family of growth-associated proteins, which also includes GAP-43, a protein recently shown to regulate neural cell adhesion molecule (NCAM)-mediated neurite outgrowth. Here, the effects of BASP1 overexpression were investigated in PC12E2 cells and pr...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21678
更新日期：2008-08-01 00:00:00
