Abstract:
:The immunohistochemical distribution of microtubule-associated protein 2 (MAP2), being normally restricted to nerve cell bodies and dendrites, became altered in rat dorsal root ganglia and spinal cord neurons in cultures infected with rhesus rotavirus. MAP2 appeared in axons of both sources of neurons as displayed with monoclonal antibodies to MAP2a + b and MAP2a + b + c at 48 hr post-infection (p.i.). Other cytoskeletal elements, i.e., tau, MAP1, MAP5, neurofilament, actin, and tubulin, did not reveal any alterations in the rotavirus-infected neurons. One of the rotavirus cytosolic proteins, the inner capsid protein vp6, was expressed in axons at 48 hr p.i. simultaneously with the appearance of MAP2, while two other viral proteins, vp4 and NS28, remained in the nerve cell bodies. By quantitative enzyme-linked immunosorbent assay (ELISA) a binding of single-shelled rotaviruses, which express vp6 on their surfaces, to purified MAP2 was found. There was no binding of these viral particles to tau or tubulin proteins. This study indicates that a selective interaction between certain viral and neuronal cytoskeletal proteins can occur and that a non-cytolytic viral infection can cause alterations in the polarized sorting of neuronal proteins.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Weclewicz K,Svensson L,Billger M,Holmberg K,Wallin M,Kristensson Kdoi
10.1002/jnr.490360207subject
Has Abstractpub_date
1993-10-01 00:00:00pages
173-82issue
2eissn
0360-4012issn
1097-4547journal_volume
36pub_type
杂志文章abstract::Tumor necrosis factor-alpha (TNF-alpha) has attracted the greatest attention as a major factor in experimental autoimmune encephalomyelitis (EAE) pathogenesis. We compared rats undergoing EAE with manipulated but healthy animals by examining TNF-alpha gene expression in cells recovered from the brain. We used reverse ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19960815)45:4<400::AID-JNR
更新日期:1996-08-15 00:00:00
abstract::Intracranial atherosclerosis (ICAS) is the most common etiology of ischemic stroke with the highest rate of stroke recurrence. Little is currently known of the association of circulating inflammation-regulatory microRNAs (miRNAs) with ICAS. In this review, we briefly discuss that ICAS is characterized as a dynamic and...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24487
更新日期:2019-10-01 00:00:00
abstract::One premise regarding the mechanism of injury-evoked neuroregeneration is that injured cells induce the release of neurotrophic factors to trigger neurogenesis. Extracellular purine nucleotides exert multiple neurotrophic actions in the central nervous system mediated via activation of purinergic receptors. However, w...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21954
更新日期:2009-05-01 00:00:00
abstract::HEPES inhibited the taurine uptake in glial cells. A different kind of inhibition was observed when HEPES was present in the culture medium or in the incubation medium used for the taurine-uptake measurement. As an example of a possible interference of HEPES in pharmacological experiments, we have studied the effect o...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490230111
更新日期:1989-05-01 00:00:00
abstract::Studies have shown that individuals with both a history of traumatic brain injury and inheritance of apolipoprotein E-4 (ApoE4) allele are associated with a poor neurologic outcome and an increased risk for Alzheimer's disease. We assessed the hypothesis that thrombin released during brain injury causes an increase in...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20887
更新日期:2006-08-01 00:00:00
abstract::The neural cell adhesion molecule L1 is a multidomain protein that plays important roles in cell adhesion, migration, and neurite outgrowth. It can interact with itself by a self-binding, i.e., homophilic adhesion mechanism (Kadmon et al.: J Cell Biol 110: 193-208, 1990a). To determine the domains of L1 involved in ho...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490420103
更新日期:1995-09-01 00:00:00
abstract::Two clones encoding human glial fibrillary acidic protein (GFAP) were isolated from a human astrocytoma cDNA library. The clones pHGFAP1 and pHGFAP2 were selected by the combined use of differential colony hybridization and hybridization-selection technique with polyclonal anti GFAP antiserum. The longer one, pHGFAP1,...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490200204
更新日期:1988-01-01 00:00:00
abstract::Male gerbils (Meriones unguiculatus) were bred and reared either grouped under enriched environmental conditions or isolated under impoverished environmental conditions. The objective of the present study was to examine the influence of social environment on structural and functional ontogeny of the medial prefrontal ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19980415)52:2<201::AID-JNR
更新日期:1998-04-15 00:00:00
abstract::Early studies of murine experimental autoimmune encephalomyelitis (EAE) induced with myelin basic protein (MBP) divide various mouse strains into either "susceptible" or "resistant" phenotypes. Resistance is defined as lack of encephalitogenic responses after active immunization or adoptive transfer. It is now becomin...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19960915)45:6<690::AID-JNR
更新日期:1996-09-15 00:00:00
abstract::Oligodendrocytes (OLs) and the myelin produced by them are enriched in two glycosphingolipids, galactosylceramide (GalC) and its sulfated form, cerebroside sulfate (CBS). We showed earlier that these two glycolipids in opposed liposomal membranes or in methanol solution can adhere to each other. Here we have examined ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.1217
更新日期:2001-10-15 00:00:00
abstract::In offspring, an adequate maternal diet is important for neurodevelopment. One mechanism by which maternal diet impacts neurodevelopment is through its dynamic role in the development of the gut microbiota. Communication between the gut, and its associated microbiota, and the brain is facilitated by the vagus nerve, i...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24605
更新日期:2021-01-01 00:00:00
abstract::Astrocytes are the most abundant glial cell in the central nervous system. They modulate synaptic function through a variety of mechanisms, and yet remain relatively understudied with respect to overall neuronal circuit function. Exploiting the tractability of the mouse olfactory system, we manipulated astrocyte activ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.24634
更新日期:2020-08-01 00:00:00
abstract::In the present study, following previous experience with electrolytic lesion of the rat brain, and subsequent reduction of reactive gliosis with 7 beta-hydroxycholesterol derivatives (Bochelen et al.: Neuroscience 51:827-834, 1992), we have performed a hemisection of the spinal cord in adult rats and investigated the ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490410110
更新日期:1995-05-01 00:00:00
abstract::Previous studies from our laboratory suggest that Alzheimer's disease sera contain a repertoire of antibodies to the heavy neurofilament subunit (NF-H) and that a subpopulation of these antibodies bind specifically to epitopes highly enriched in NF-H isolated from the purely cholinergic electromotor neurons of Torpedo...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490290409
更新日期:1991-08-01 00:00:00
abstract::Primary brain tumors, particularly glioblastomas (GB), remain a challenge for oncology. An element of the malignant brain tumors' aggressive behavior is the fact that GB are among the most densely vascularized tumors. To determine some of the molecular regulations occuring at the brain tumor endothelium level during t...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10865
更新日期:2004-01-15 00:00:00
abstract::Pigment epithelium-derived factor (PEDF) is a neurotrophic protein synthesized and secreted by retinal pigment epithelial (RPE) cells in early embryogenesis and has been shown to be present in the extracellular matrix between the RPE cells and the neural retina. It induces neuronal differentiation and promotes surviva...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1999-09-15 00:00:00
abstract::The neural cell adhesion molecule (NCAM) plays a key role in neural development, regeneration, and synaptic plasticity. The crystal structure of a fragment of NCAM comprising the three N-terminal immunoglobulin (Ig)-like modules indicates that the first and second Ig modules bind to each other, thereby presumably medi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22380
更新日期:2010-08-01 00:00:00
abstract::We have previously shown in rat primary cultured mesencephalic cells that cyanide induces a high level of oxidative stress and necrotic death. To evaluate the mechanism of the cytotoxicity, the effects of cyanide on intracellular glutathione (GSH) pools and inducible nitric oxide synthase (iNOS)-mediated reactive nitr...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20998
更新日期:2006-10-01 00:00:00
abstract::Previous studies on the NG2 chondroitin sulfate proteoglycan have shown that NG2 is expressed on A2B5-positive O2A progenitor cells, which are known to respond to platelet-derived growth factor (PDGF). In the accompanying paper (Nishiyama et al.; J Neurosci Res 43:299-314, 1996) we show that on O2A progenitors in the ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19960201)43:3<315::AID-JNR
更新日期:1996-02-01 00:00:00
abstract::Corticosteroids, released from the adrenal gland in response to stress, bind to receptors that act as transcription factors to alter gene expression and, subsequently, protein synthesis. Using [(35)S]-methionine-cysteine incorporation to measure protein synthesis in hippocampal slices incubated under ischemic conditio...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.1159
更新日期:2001-08-15 00:00:00
abstract::An angiotensin 2 type 1 receptor blocker, olmesartan, and a calcium channel blocker, azelnidipine, possess not only an antihypertensive effect but also an antioxidative effect and other beneficial effects. In the present study, we examined the efficacy of olmesartan and azelnidipine monotherapy (2 mg/kg or 10 mg/kg ea...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23406
更新日期:2014-10-01 00:00:00
abstract::Four major discoveries on the function of the pedunculopontine nucleus (PPN) have significantly advanced our understanding of the role of arousal in neurodegenerative disorders. The first was the finding that stimulation of the PPN-induced controlled locomotion on a treadmill in decerebrate animals, the second was the...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24417
更新日期:2019-12-01 00:00:00
abstract::Laminin mediates neural adhesion and process formation. A possible signal transduction pathway for laminin was investigated in both NG108-15 and PC12 neuronal cells using radiolabeling studies as well as various stimulators and inhibitors of phosphatases and kinases. Using [32P]-ortho-phosphate, laminin caused a decre...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490270321
更新日期:1990-11-01 00:00:00
abstract::Seventeen white patients from the Stanford Sleep Disorders Clinic complaining of excessive daytime somnolence (EDS) were selected for restriction-fragment-length polymorphism (RFLP) studies. Fourteen of the patients with clinically diagnosed narcolepsy were seropositive for DR2. RFLP analysis of these patients compare...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490180134
更新日期:1987-01-01 00:00:00
abstract::Calcium-activated neutral proteinase (CANP) activity was determined in cytosolic and membranous subcellular fractions of transformed Schwann cells (tSc). The muM and mM Ca(2+)-sensitive (mu- and mCANP) forms of CANP were separated by DEAE and phenyl Sepharose column chromatography, the latter step enabling removal of ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490290310
更新日期:1991-07-01 00:00:00
abstract::By using two different approaches, ubiquitin C-terminal hydrolase 1 (UCHL1) was identified as a potential cerebrospinal fluid (CSF) biomarker of neuronal loss in aneurysmal subarachnoid hemorrhage (ASAH) and presumably other CNS damage and disease states. Appropriate antibodies and a sensitive ELISA were generated, an...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22323
更新日期:2010-05-15 00:00:00
abstract::Glial cell line-derived neurotrophic factor (GDNF) acts as a potent survival factor for many neuronal populations, including spinal motoneurons, indicating the therapeutic promise of GDNF for neurological disorders. Injury to spinal cord (SCI) triggers processes destructive to ascending sensory and descending motor co...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10303
更新日期:2002-08-01 00:00:00
abstract::Physiological responses to acute stress proceed with the activation of the hypothalamus-pituitary-adrenal gland (HPA) system. Many brain regions are known to modulate the HPA axis activation in stress responses, but the detailed neural circuits and signaling system in the upstream of the HPA axis have to be explored f...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22119
更新日期:2009-10-01 00:00:00
abstract::The periglomerular cells (PG) of the olfactory bulb (OB) are involved in the primary processing and the refinement of sensory information from the olfactory epithelium. The neurochemical composition of these neurons has been studied in depth in many species, and over the last decades such studies have focused mainly o...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22521
更新日期:2011-01-01 00:00:00
abstract::A link between aluminum (Al) exposure and age-related neurological disorders has long been proposed. Although the exact mechanism by which the metal may influence disease processes is unknown, there is evidence that exposure to Al causes an increase in both oxidative stress and inflammatory events. These processes hav...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10877
更新日期:2004-02-15 00:00:00