Abstract：
:By using two different approaches, ubiquitin C-terminal hydrolase 1 (UCHL1) was identified as a potential cerebrospinal fluid (CSF) biomarker of neuronal loss in aneurysmal subarachnoid hemorrhage (ASAH) and presumably other CNS damage and disease states. Appropriate antibodies and a sensitive ELISA were generated, and the release of UCHL1 into CSF was compared with that of pNF-H and S100beta in a cohort of 30 ASAH patients. Both UCHL1 and pNF-H showed persistent release into CSF in almost all patients in the second week postaneurysmal rupture (AR), and S100beta levels rapidly declined to baseline levels in 23 of 30 patients. Seven of thirty patients showed persistently elevated S100beta levels over the first 5 days post-AR and also had relatively higher levels of pNF-H and UCHL1 higher compared with the rest. These patients proved to have very poor outcomes, with 6 of 7 expiring. Patients who did reduce S100beta levels tended to have a better outcome if pNF-H and UCHL1 levels were also lower, and elevated UCHL1 levels in the second week post-AR were particularly predictive of poor outcome. Acute coordinated releases of large amounts of UCHL1, pNF-H, and S100beta in 16 of 30 patients were observed, suggesting sudden loss of brain tissues associated with secondary events. We conclude that measurement of the CSF levels of these proteins reveals details of ASAH progression and recovery and predicts patient outcome.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Lewis SB,Wolper R,Chi YY,Miralia L,Wang Y,Yang C,Shaw Gdoi
10.1002/jnr.22323subject
Has Abstractpub_date
2010-05-15 00:00:00pages
1475-84issue
7eissn
0360-4012issn
1097-4547journal_volume
88pub_type
杂志文章abstract：:The neural cell adhesion molecule (NCAM) plays a key role in neural development, regeneration, and synaptic plasticity. The crystal structure of a fragment of NCAM comprising the three N-terminal immunoglobulin (Ig)-like modules indicates that the first and second Ig modules bind to each other, thereby presumably medi...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22380
更新日期：2010-08-01 00:00:00
abstract：:Neuron expression of the cytokine tumor necrosis factor-alpha (TNF), and the regulation of the levels of TNF by alpha(2)-adrenergic receptor activation were investigated. Adult rat hippocampal neurons and phorbol ester (PMA)-differentiated SH-SY5Y cells were examined. Intracellular levels of TNF mRNA accumulation, as ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10101
更新日期：2002-01-15 00:00:00
abstract：:Hippocalcin is a Ca2+-binding protein that is expressed mainly in pyramidal nerve cells of the hippocampus. However, its functions and mechanism in the brain remain unclear. To elucidate the role of hippocalcin, we used a conditionally immortalized hippocampal cell line (H19-7) and showed that bFGF treatment increased...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21602
更新日期：2008-05-15 00:00:00
abstract：:Heme oxygenase-1 (HO1) catalyzes oxidation of the heme molecule in concert with NADPH-cytochrome P450 reductase following the specific cleavage of heme into carbon monoxide, iron, and biliverdin, which is rapidly metabolized to bilirubin. HO1 is a stress-inducible protein that protects cells against oxidative injury, ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10514
更新日期：2003-02-15 00:00:00
abstract：:The mechanisms by which polyglutamine expansion causes common features of neuronal death remain unclear. Here we describe an approach for delivering polyglutamine expansions directly into cultured sympathetic neurons. Glutamine (Q) residues (n = 10, 22, 30) were conjugated with a peptide possessing translocation prope...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20461
更新日期：2005-05-15 00:00:00
abstract：:Our previous results support the idea that CREB (cyclic AMP-response element binding protein) may be a mediator of neuroligand and growth factor signals that, coupled to different signal transduction pathways, play different roles at specific stages of oligodendrocyte development. In the early stages, when cells are i...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.1195
更新日期：2001-10-01 00:00:00
abstract：:Using morphological, immunocytochemical, and functional parameters we have previously shown that highly purified adult rat microglial cells undergo a process of "activation" when cultured in a serum-containing medium in the absence of added proinflammatory substances or other factors (Slepko and Levi: Glia 16:241-246,...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(sici)1097-4547(19970801)49:3<292::aid-jnr
更新日期：1997-08-01 00:00:00
abstract：:The interaction of bovine white matter proteolipid apoproteins with sodium dodecyl sulfate (SDS) was studied. Equilibrium dialysis binding measurements show that the apoprotein binds approximately 1.5 gm SDS per gram protein at high ionic strength (gamma/2 = 0.17). At low ionic strength (gamma/2 = 0.01) the protein bi...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490060602
更新日期：1981-01-01 00:00:00
abstract：:Studies have shown that individuals with both a history of traumatic brain injury and inheritance of apolipoprotein E-4 (ApoE4) allele are associated with a poor neurologic outcome and an increased risk for Alzheimer's disease. We assessed the hypothesis that thrombin released during brain injury causes an increase in...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20887
更新日期：2006-08-01 00:00:00
abstract：:Neuronal cell differentiation alterations induced by mutant presenilin 2 (PS2) were investigated in transgenic mice expressing wild-type or mutant-type PS2. Progressive increases in differentiation and marker protein expression were found in neuronal cells expressing wild-type PS2, whereas these processes were much pe...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20655
更新日期：2005-11-15 00:00:00
abstract：:The extension of filopodia from growth cones of regenerating neurites from rat superior cervical ganglion neurons in tissue culture was studied. Cultures were grown on a thin layer of fibrous collagen and maintained in a medium containing serum and nerve growth factor. Time-lapse cinematography and computer-assisted m...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490130111
更新日期：1985-01-01 00:00:00
abstract：:Abdominal muscle electromyograms were monitored in response to graded mechanical loads added to either inspiration or expiration in anesthetized cats. The surgical preparation, the apparatus, and the levels of loads applied (resistive, tracheal occlusion, and continuous positive pressure) were matched with those emplo...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490130413
更新日期：1985-01-01 00:00:00
abstract：:Because there is a strong correlation between tuberous sclerosis and autism, we used a tuberous sclerosis model (Eker rat) to test the hypothesis that these animals would have an altered regional cerebral O2 consumption that might be associated with autism. We also examined whether the altered cerebral O2 consumption ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21378
更新日期：2007-08-15 00:00:00
abstract：:A study was conducted on the adsorption of 14C-ATP to a surface film of a hydrophobic protein derived from synaptic membranes isolated from bovine cerebellum. The adsorption of ATP to the protein film followed a rate law based on diffusion and an energy barrier to adsorption, the rate law being generally applicable to...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490020109
更新日期：1976-01-01 00:00:00
abstract：:Germinal matrix (GM) vasculature is selectively vulnerable to hemorrhage in premature infants during the first 48 hr of life. This is attributed to rapid angiogenesis of this brain region, resulting in formation of nascent vessels that show a paucity of pericytes and immaturity of extracellular matrix. Integrins are k...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22301
更新日期：2010-05-01 00:00:00
abstract：:Butyrate produced a biphasic modulation of the thyroid hormone receptor in neuroblastoma N2A cells increasing receptor number by 20-35% at concentrations 0.25-0.75 mM and decreasing receptor levels by 30-55% at 2-4 mM. The half-life of the receptor, as assessed by its disappearance after incubation with 18 microM cycl...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490270102
更新日期：1990-09-01 00:00:00
abstract：:The thrombospondin (TSP) family of extracellular matrix glycoproteins are widely expressed in the developing and adult central nervous system although their function remains poorly defined. We have used cell culture techniques to analyse the expression and function of TSPs in glial cells derived from myelinated region...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19971015)50:2<202::AID-JNR
更新日期：1997-10-15 00:00:00
abstract：:Long-duration exposure to weightlessness results in bone demineralization, muscle atrophy, cardiovascular deconditioning, altered sensory-motor control, and central nervous system reorganizations. Exercise countermeasures and body loading methods so far employed have failed to prevent these changes. A human mission to...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/1097-4547(20001015)62:2<169::AID-JNR2>3.0.
更新日期：2000-10-15 00:00:00
abstract：:The amygdala contributes to the generation and propagation of epileptiform activity in temporal lobe epilepsy (TLE). Ictal symptoms such as fear, dreamy states (déjà vu, memory flashbacks, experiential hallucinations), epigastric auras, or sympathetic outflow with cardiovascular changes are often linked to a seizure f...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.23689
更新日期：2016-06-01 00:00:00
abstract：:Respiration was measured polarographically in astrocytes from dissociated neonatal rat cerebra grown in primary culture. Cells grown in a modified Eagle's minimal essential medium containing 20% fetal calf serum for 14 to 21 days respired at a rate of 34.23 n at. equiv 0 mg protein-1 min-1. Cells which were grown in t...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490050605
更新日期：1980-01-01 00:00:00
abstract：:Several recently discovered lines of evidence support the involvement of myelin basic protein (BP)-specific T cells in multiple sclerosis (MS). To identify potentially relevant immunodominant T cell epitopes, human BP (Hu-BP)-reactive T cell lines were selected from MS and normal donors and tested for reactivity to cl...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490280215
更新日期：1991-02-01 00:00:00
abstract：:Topoisomerase IIβ (top IIβ) is a nuclear enzyme with an essential role in neural development. The regulation of top IIβ gene expression during neural differentiation is poorly understood. Functional analysis of top IIβ gene structure displayed a GC box sequence in its transcription promoter, which binds the nuclear tr...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23403
更新日期：2014-10-01 00:00:00
abstract：:The limited availability and potential to culture primary human brain cells means that there is still a need for cell lines that reliably model human neurons and glial cells. The human-derived NTera2/D1 (NT2) cell line is a promising tool from which both neuronal (NT2N) and astrocytic (NT2A) cells can be derived in vi...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22692
更新日期：2011-10-01 00:00:00
abstract：:Fibroblast growth factor 2 (FGF2) exhibits pleiotropic functions during embryogenesis. In neural development, both pro- and antineurogenic activities of FGF2 have been described in the differentiation of neuronal progenitors into postmitotic neurons. We used cultured neural stem cells (NSCs) derived from rat embryonic...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20627
更新日期：2005-10-15 00:00:00
abstract：:We describe here the presence of ubiquitin and its conjugation system in the rat retina. Retinal homogenates and supernatants conjugate [125I]human ubiquitin with either endogenous or exogenous proteins. The conjugating activity is relatively stable over time, requires ATP, and has a pH optimum of approximately 8. The...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490300220
更新日期：1991-10-01 00:00:00
abstract：:In chronic demyelinating lesions of the central nervous system, insufficient generation of oligodendrocytes (OLs) is not due to a lack of oligodendrocyte precursor cells (OPCs), because the accumulation of OPCs and premyelinating OLs can be observed within these lesions. Here we sought to identify the basis for the fa...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23153
更新日期：2013-02-01 00:00:00
abstract：:Calpain, an intracellular cysteine protease, has been widely reported to be involved in neuronal cell death. The purpose of this study is to investigate the role of calpain activation in axonal damage-induced retinal ganglion cell (RGC) death. Twelve-week-old male calpstatin (an endogenous calpain inhibitor) knockout ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22800
更新日期：2012-04-01 00:00:00
abstract：:The development of cells of the oligodendroglial lineage, from immature progenitor to myelinating cells, occurs largely in a neuronal environment, yet little is known about specific interactions between these 2 cell types. We have tested the effects of medium conditioned by cultures of rat cerebral cortical neurons (C...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490340510
更新日期：1993-04-01 00:00:00
abstract：:gamma-Aminobutyric acid (GABA) regulates the proliferation and migration of olfactory bulb (OB) interneuron progenitors derived from the subventricular zone (SVZ), but the role of GABA in the differentiation of these progenitors has been largely unexplored. This study examines the role of GABA in the differentiation o...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22055
更新日期：2009-08-01 00:00:00
abstract：:Soluble amyloid beta protein (Abeta) oligomers have been considered recently to be responsible for the cognitive dysfunction that sets in prior to senile plaque formation in the Alzheimer's disease (AD) brain. By using the newly prepared antibody against oligomer Abeta, rather than fibrillar or monomer Abeta, we obser...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20952
更新日期：2006-08-15 00:00:00
