Induction of myelin basic protein-specific experimental autoimmune encephalomyelitis in C57BL/6 mice: mapping of T cell epitopes and T cell receptor V beta gene segment usage.

Abstract:

:Early studies of murine experimental autoimmune encephalomyelitis (EAE) induced with myelin basic protein (MBP) divide various mouse strains into either "susceptible" or "resistant" phenotypes. Resistance is defined as lack of encephalitogenic responses after active immunization or adoptive transfer. It is now becoming clear that this unresponsiveness is not due to the inability of T cells to recognize MBP in the context of major histocompatibility complex (MHC) gene products. Using various manipulations, many laboratories are able to induce severe EAE in these strains. We previously reported that a combination of adoptive transfer and subsequent challenge of the recipients with MBP could overcome the resistance in many mouse strains (Shaw et al.: J Neuroimmunol 39:139-150, 1992). This approach now enables us to identify the encephalitogenic epitope and T cell receptor V beta usage in a prototype strain, C57BL/6 (B6). Pepsin-digested MBP fragments first located a major T cell epitope in a polypeptide containing residues 44-88. Overlapping synthetic peptides narrowed this epitope to p60-80. Truncated peptides from the carboxyl- or amino-terminus further mapped a minimal peptide to p67-76. This encephalitogenic epitope appears to be unique to B6 mice. Independent encephalitogenic T cell clones specific for this epitope were also generated. Of six such clones analyzed, five different TCR V beta's were found. Whether unbiased usage of encephalitogenic TCR V beta gene segments in B6 mice is related to its EAE resistant phenotype is not clear at this point.

journal_name

J Neurosci Res

authors

Shaw MK,Kim C,Hao HW,Chen F,Tse HY

doi

10.1002/(SICI)1097-4547(19960915)45:6<690::AID-JNR

subject

Has Abstract

pub_date

1996-09-15 00:00:00

pages

690-9

issue

6

eissn

0360-4012

issn

1097-4547

pii

10.1002/(SICI)1097-4547(19960915)45:6<690::AID-JNR

journal_volume

45

pub_type

杂志文章
  • Effects of oral tolerance induction by myelin basic protein on Vbeta8+ Lewis rat T cells.

    abstract::Encephalitogenic T cells from Lewis rats use a restricted T cell receptor (TCR) gene combination, Vbeta8.2 and Valpha2. The oral administration of myelin basic protein (MBP) to Lewis rats prior to encephalitogenic challenge results in a marked inhibition of clinical neurologic signs of encephalitis, reduced central ne...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4547(19980101)51:1<67::AID-JNR7

    authors: Goldman-Brezinski S,Brezinski K,Zhang XM,Gienapp I,Cox K,Heber-Katz E,Whitacre C

    更新日期:1998-01-01 00:00:00

  • Hyposmolarity-induced taurine release in cerebellar granule cells is associated with diffusion and not with high-affinity transport.

    abstract::The effects of hyposmotic conditions on taurine uptake and release were studied in mice cultured cerebellar granule cells. The effect of DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulfonate) and of the divalent cations Mg++ and Mn++ on the hyposmolarity-induced changes in these parameters was investigated. Mg++ (20 mM...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490300409

    authors: Schousboe A,Sánchez Olea R,Morán J,Pasantes-Morales H

    更新日期:1991-12-01 00:00:00

  • Divalent cations modulate the activity of metabotropic glutamate receptors.

    abstract::Metabotropic glutamate receptors (mGluRs) and calcium receptors (CaR) are closely related G protein-coupled receptors (GPCRs). The similar structural and functional properties of mGluRs and CaRs include conserved amino acid residues involved in glutamate binding in mGluRs and Ca2+ binding in the CaR. Furthermore, rece...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.10853

    authors: Francesconi A,Duvoisin RM

    更新日期:2004-02-15 00:00:00

  • Downregulation of IL-10 secretion and enhanced antigen-presenting abilities following HTLV-I infection of T cells.

    abstract::Human T-cell lymphotropic virus type I (HTLV-I) may infect up to 10% of peripheral blood T cells in patients with HTLV-I myelopathy. To examine the impact of HTLV-I infection on the abilities of T cells to present and respond to peptide antigen, we HTLV-I infected and subcloned a myelin basic protein peptide 84-102 (M...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4547(19960915)45:6<786::AID-JNR

    authors: Scholz C,Hafler DA,Höllsberg P

    更新日期:1996-09-15 00:00:00

  • Nerve growth factors in chick tissues.

    abstract::Bioassays of factors promoting nerve growth in embryonic ganglia are described. Chick embryo extract evokes fiber outgrowth in both sympathetic and ciliary ganglia explanted into a collagen gel. The response is not suppressed by antibodies directed against mouse nerve growth factor (NGF). Chick embryo extract also sup...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490080206

    authors: Ebendal T,Hedlund KO,Norrgren G

    更新日期:1982-01-01 00:00:00

  • Overexpression of Akt (protein kinase B) confers protection against apoptosis and prevents formation of ceramide in response to pro-apoptotic stimuli.

    abstract::An immortalized dorsal root ganglion cell line F-11 exhibits many properties of spinal cord neurons and undergoes apoptosis in response to growth factor withdrawal and the exogenous addition of inhibitors of phosphatidylinositol-3-kinase (PI3K). To elucidate the mechanism of apoptosis we generated F-11 clones which ov...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:

    authors: Goswami R,Kilkus J,Dawson SA,Dawson G

    更新日期:1999-09-15 00:00:00

  • Cobalt-dependent potentiation of net inward current density in Helix aspersa neurons.

    abstract::A low concentration of transition metal ions Co2+ and Ni2+ increases the inward current density in neurons from the land snail Helix aspersa. The currents were measured using a single electrode voltage-clamp/internal perfusion method under conditions in which the external Na+ was replaced by Tris+, the predominant ext...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490280411

    authors: Kim YK,Woodruff ML

    更新日期:1991-04-01 00:00:00

  • Role of N-methyl-D-aspartate receptors in gastric mucosal blood flow induced by histamine.

    abstract::Ionotropic N-methyl-D-aspartate (NMDA) receptor agonists, L-aspartic acid (L-Asp) and NMDA, have been shown to inhibit histamine-stimulated acid secretion, but their effect on gastric mucosal blood flow (GMBF) is largely unknown. The aim of this study was to investigate whether L-Asp and NMDA inhibit histamine-stimula...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.20202

    authors: Tsai LH,Lee YJ,Wu JY

    更新日期:2004-09-01 00:00:00

  • Genetic elevation of monoamine oxidase levels in dopaminergic PC12 cells results in increased free radical damage and sensitivity to MPTP.

    abstract::Production of hydrogen peroxide as a by-product of the breakdown of catecholamines by the enzyme monoamine oxidase (MAO) has been hypothesized to contribute to the increased proclivity of dopaminergic neurons for oxidative injury. We established clonal dopaminergic PC12 cell lines which have elevated MAO activity leve...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4547(19961215)46:6<666::AID-JNR

    authors: Wei Q,Yeung M,Jurma OP,Andersen JK

    更新日期:1996-12-15 00:00:00

  • Calcium-activated neutral protease in the peripheral nerve, which requires microM order Ca2+, and its effect on the neurofilament triplet.

    abstract::There are two types of calcium-activated neutral protease (CANP), m-CANP and mu-CANP, following the nomenclature of Suzuki et al to show that each requires mM and microM Ca2+, respectively, for its activation. We found mu-CANP activity in a crude CANP fraction extracted from the peripheral nerve, which degraded the ne...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490130306

    authors: Kamakura K,Ishiura S,Suzuki K,Sugita H,Toyokura Y

    更新日期:1985-01-01 00:00:00

  • Effects of alcohol on brain-derived neurotrophic factor mRNA expression in discrete regions of the rat hippocampus and hypothalamus.

    abstract::Chronic alcohol consumption has adverse effects on the central nervous system, affecting some hippocampal and hypothalamic functions. In this study we tempted to demonstrate that some of these modifications could involve impairment of neurotrophic factors. Three experimental groups of male Sprague Dawley rats were stu...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/1097-4547(20010115)63:2<200::AID-JNR1012>3

    authors: Tapia-Arancibia L,Rage F,Givalois L,Dingeon P,Arancibia S,Beaugé F

    更新日期:2001-01-15 00:00:00

  • Selective changes in the permeability of the blood-brain barrier after pharmacological or surgical deafferentation.

    abstract::Unilateral eye removal results in an increased delivery of blood-borne glucose, norepinephrine, choline, and tyrosine, but not of proline, to the denervated optic lobes. Administration of reserpine to chicks increases penetrance of norepinephrine into the brain but not of tyrosine, choline, or proline. Selective alter...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490010308

    authors: Walker JB,Walker JP,Bondy SC,Purdy J

    更新日期:1975-01-01 00:00:00

  • Lipid raft-targeted Akt promotes axonal branching and growth cone expansion via mTOR and Rac1, respectively.

    abstract::The molecular mechanisms by which extracellular guidance cues regulate axonal morphology are not fully understood. Recent findings suggest that increased activity of the protein kinase Akt promotes dendritic branching and elongation in hippocampal neurons. We tested whether expression of constitutively active Akt (CA-...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.22140

    authors: Grider MH,Park D,Spencer DM,Shine HD

    更新日期:2009-11-01 00:00:00

  • Protective effect of L-trans-pyrrolidine-2,4-dicarboxilic acid preload against cell death induced by oxygen/glucose deprivation in differentiated PC12 cells.

    abstract::It has been postulated that cellular glutamate is released into the extracellular fluid when the energy supply of the brain is compromised (i.e., anoxia or oxygen/glucose deprivation), and there the amino acid triggers the so-called excitotoxic cascade, causing neuronal death. Several mechanisms for this release have ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.20613

    authors: Martín ME,Muñoz FM,Dickinson DA,Forman HJ,Martín del Río R,Salinas M,Fando JL

    更新日期:2005-10-01 00:00:00

  • Comparative study of cell culture and purification methods to obtain highly enriched cultures of proliferating adult rat Schwann cells.

    abstract::We present here a fast protocol that could be used to obtain highly purified cultures of maximal proliferating adult rat Schwann cells. These adult rat Schwann cells can be transfected in a nonbiological way using the physical transfection method of electroporation. Schwann cells are decisive in recovery of peripheral...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.20166

    authors: Mauritz C,Grothe C,Haastert K

    更新日期:2004-08-01 00:00:00

  • Regulation of A1 adenosine receptor functioning induced by P2Y1 purinergic receptor activation in human astroglial cells.

    abstract::In the rat brain, a heteromeric association between adenosine A(1) and purinergic P2Y(1) receptors has been demonstrated. It is suggested that this association plays an important role in the control of purine-mediated responses during pathophysiological conditions. Recently, we have demonstrated that these receptors c...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.21727

    authors: Tonazzini I,Trincavelli ML,Montali M,Martini C

    更新日期:2008-10-01 00:00:00

  • Aberrant features of in vivo striatal dynamics in Parkinson's disease.

    abstract::The striatum plays an important role in learning, selecting, and executing actions. As a major input hub of the basal ganglia, it receives and processes a diverse array of signals related to sensory, motor, and cognitive information. Aberrant neural activity in this area is implicated in a wide variety of neurological...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审

    doi:10.1002/jnr.24519

    authors: Lee K,Masmanidis SC

    更新日期:2019-12-01 00:00:00

  • The application of in vitro-derived human neurons in neurodegenerative disease modeling.

    abstract::The development of safe and effective treatments for age-associated neurodegenerative disorders is an on-going challenge faced by the scientific field. Key to the development of such therapies is the appropriate selection of modeling systems in which to investigate disease mechanisms and to test candidate intervention...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审

    doi:10.1002/jnr.24615

    authors: D'Souza GX,Rose SE,Knupp A,Nicholson DA,Keene CD,Young JE

    更新日期:2021-01-01 00:00:00

  • 1-[2-(4-Benzyloxyphenoxy)Ethyl]Imidazole inhibits monoamine oxidase B and protects against neuronal loss and behavioral impairment in rodent models of Parkinson's disease.

    abstract::Monoamine oxidase B (MAO-B) is well known as a therapeutic target for Parkinson's disease (PD). MAO-B inhibitors retain antiparkinsonism abilities to improve motor function and prevent neuronal loss by decreasing dopamine metabolism and oxidative stress in the brain. From the study to find novel antiparkinsonism drugs...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.23577

    authors: Chung JY,Lee JW,Ryu CH,Min HK,Yoon YJ,Lim MJ,Park CH

    更新日期:2015-08-01 00:00:00

  • Maintenance of glial plasticity with aging in C-6 glial cells and normal astrocytes in culture: responsiveness to opioid peptides.

    abstract::In this study we used as glial cell models, early and late passage C-6 glial cells, 2B clone, and advanced passages of glial cells derived from aged mouse cerebral hemispheres (MACH) to examine responsiveness to opioids. We have previously reported that early passage C-6 glial cells, 2B clone, are bipotential and can ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490360509

    authors: Kozlova M,Kentroti S,Vernadakis A

    更新日期:1993-12-01 00:00:00

  • Noninvasive early detection of brain edema in mice by near-infrared light scattering.

    abstract::Brain edema accounts for significant morbidity and mortality in many neurologic conditions such as head trauma, stroke, meningitis, and brain tumor. The water channel aquaporin-4 (AQP4) has been found to be an important determinant of brain water accumulation and clearance of excess brain water. We report the developm...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.20439

    authors: Thiagarajah JR,Papadopoulos MC,Verkman AS

    更新日期:2005-04-15 00:00:00

  • Glial growth is regulated by agonists selective for multiple opioid receptor types in vitro.

    abstract::To determine whether one or more opioid receptor types might be preferentially involved in gliogenesis, primary mixed glial cultures derived from mouse cerebra were continuously treated with varying concentrations of opioid agonists selective for mu (mu), i.e., DAGO ([D-Ala2, MePhe4, Gly(ol)5]enkephalin), delta (delta...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490290415

    authors: Stiene-Martin A,Hauser KF

    更新日期:1991-08-01 00:00:00

  • Growth and pathfinding of regenerating axons in the optic projection of adult fish.

    abstract::In contrast to mammals, teleost fish are able to regrow severed long-range projection axons in the central nervous system (CNS), leading to recovery of function. The optic projection in teleost fish is used to study neuron-intrinsic and environmental molecular factors that determine successful axon regrowth and naviga...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审

    doi:10.1002/jnr.21121

    authors: Becker CG,Becker T

    更新日期:2007-09-01 00:00:00

  • Intensity of chronic cerebral hypoperfusion determines white/gray matter injury and cognitive/motor dysfunction in mice.

    abstract::We sought to establish a mouse model of subcortical ischemic vascular dementia (SIVD) that develops predominant white matter (WM) injury and cognitive dysfunction induced by chronic cerebral hypoperfusion. Adult C57Bl/6 male (n = 48) mice were subjected to bilateral common carotid artery stenosis with external microco...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.21925

    authors: Miki K,Ishibashi S,Sun L,Xu H,Ohashi W,Kuroiwa T,Mizusawa H

    更新日期:2009-04-01 00:00:00

  • Localization of proopiomelanocortin mRNA in functional subsets of neurons defined by their axonal projections.

    abstract::In situ cDNA:mRNA hybridization is a technique that has been developed for the visualization of cDNA:mRNA hybrids in individual cells. To use this technique to answer questions of regulation in heterogeneous populations of cells in the brain, it must be combined with other procedures allowing for the identification of...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490160109

    authors: Wilcox JN,Roberts JL,Chronwall BM,Bishop JF,O'Donohue T

    更新日期:1986-01-01 00:00:00

  • Liquid diet induces memory impairment accompanied by a decreased number of hippocampal neurons in mice.

    abstract::It is suggested that masticatory dysfunction affects the central nervous system; however, the underlying mechanism remains unknown. Brain-derived neurotrophic factor (BDNF) and its receptor, TrkB, are known to play important roles in memory and learning. In this study, we examined the effects of mastication on memory,...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.23383

    authors: Okihara H,Ito J,Kokai S,Ishida T,Hiranuma M,Kato C,Yabushita T,Ishida K,Ono T,Michikawa M

    更新日期:2014-08-01 00:00:00

  • Alterations in ciliary neurotrophic factor signaling in rapsyn deficient mice.

    abstract::Rapsyn is a key molecule involved in the formation of postsynaptic specializations at the neuromuscular junction, in its absence there are both pre- and post-synaptic deficits including failure to cluster acetylcholine receptors. Recently we have documented increases in both nerve-muscle branching and numbers of moton...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.1109

    authors: Bartlett SE,Banks GB,Reynolds AJ,Waters MJ,Hendry IA,Noakes PG

    更新日期:2001-06-15 00:00:00

  • Apolipoprotein E expression after spinal cord injury in the mouse.

    abstract::Apolipoprotein E (apo-E), a protein involved in lipid metabolism and cholesterol transport, has been found to be up-regulated in CNS injury and is associated with Alzheimer's disease in humans. In this study, we show that apo-E is also up-regulated after complete spinal cord transection in the C57BL/6 mouse. In the un...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.10482

    authors: Seitz A,Kragol M,Aglow E,Showe L,Heber-Katz E

    更新日期:2003-02-01 00:00:00

  • Pathogenesis of cerebral vasospasm following aneurysmal subarachnoid hemorrhage: putative mechanisms and novel approaches.

    abstract::Cerebral vasospasm is a potentially incapacitating or lethal complication in patients with aneurysmal subarachnoid hemorrhage (SAH). The development of effective preventative and therapeutic interventions has been largely hindered by the fact that the underlying pathogenic mechanisms of cerebral vasospasm remain poorl...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审

    doi:10.1002/jnr.21823

    authors: Kolias AG,Sen J,Belli A

    更新日期:2009-01-01 00:00:00

  • Projection target-specific action of nicotine in the caudal nucleus of the solitary tract.

    abstract::The brainstem nucleus of the solitary tract (NTS) is the key integrating relay in the central processing of sensory information from the thoracic and from most subdiaphragmatic viscera. Modulation of neuronal excitability and synaptic activity in the NTS by nicotinic agents can have potent effects on vital physiologic...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.23436

    authors: Feng L,Uteshev VV

    更新日期:2014-11-01 00:00:00