Abstract：
:Neural stem cells can be propagated in culture as neurospheres, yielding neurons and glial cells upon differentiation. Although the neurosphere model is widely used, the functional properties of the neurosphere-derived neurons have been only partially characterized, and it is unclear whether repeated passaging alters their functional properties. In this study, we analyzed voltage- and transmitter-gated responses in neuron-like cells obtained by differentiating fetal mouse neurospheres at increasing passages in culture. We report that neurons fire overshooting action potentials in response to depolarizing currents up to passage 10 but loose this capability at later passages, as the density of voltage-gated Na(+) and K(+) currents decreases. In contrast, the immunoreactivity for the neuronal marker beta-tubulin remains unaltered up to passage 21, indicating that this marker is not representative of cell function. In almost all neurons, gamma-aminobutyric acid (GABA) evoked bicuculline-sensitive whole-cell currents, resulting from the activation of GABA(A) receptors, which appeared to be excitatory, insofar as the reversal potential of GABA-gated current was about -50 mV. Much smaller currents were elicited by the glutamatergic agonist AMPA, and only occasional responses to glycine were detected. In these functional aspects, neurosphere-derived neurons are similar to immature neurons differentiating in vivo. Therefore, at least for a limited number of passages in vitro, neurospheres provide an adequate model of in vivo neurogenesis.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Pagani F,Lauro C,Fucile S,Catalano M,Limatola C,Eusebi F,Grassi Fdoi
10.1002/jnr.20835keywords:
subject
Has Abstractpub_date
2006-06-01 00:00:00pages
1494-501issue
8eissn
0360-4012issn
1097-4547journal_volume
83pub_type
杂志文章abstract：:The turmeric derivative curcumin protects against cerebral ischemic injury. We previously demonstrated that curcumin activates peroxisome proliferator-activated receptor-γ (PPARγ), a ligand-activated transcription factor involved in both neuroprotective and anti-inflammatory signaling pathways. This study tested wheth...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490400409
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
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journal_title：Journal of neuroscience research
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pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19960915)45:6<786::AID-JNR
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journal_title：Journal of neuroscience research
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更新日期：2003-05-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490210107
更新日期：1988-09-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/1097-4547(20000901)61:5<508::AID-JNR5>3.0.
更新日期：2000-09-01 00:00:00
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journal_title：Journal of neuroscience research
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doi：10.1002/jnr.21279
更新日期：2007-05-15 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：
更新日期：1997-04-15 00:00:00
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journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
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journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
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doi：10.1002/jnr.490390105
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journal_title：Journal of neuroscience research
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更新日期：2010-05-01 00:00:00
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更新日期：2001-01-01 00:00:00
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journal_title：Journal of neuroscience research
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abstract：:Intracranial atherosclerosis (ICAS) is the most common etiology of ischemic stroke with the highest rate of stroke recurrence. Little is currently known of the association of circulating inflammation-regulatory microRNAs (miRNAs) with ICAS. In this review, we briefly discuss that ICAS is characterized as a dynamic and...
journal_title：Journal of neuroscience research
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journal_title：Journal of neuroscience research
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更新日期：2004-12-15 00:00:00
