Abstract:
:The effects of glucose and O2 deprivation (OGD) on the survival of cortical and cerebellar neurons were examined to characterize the biochemical mechanisms involved in OGD and OGD followed by reoxygenation. To this aim, neurons were kept for different time periods in a hypoxic chamber with a controlled atmosphere of 95% N(2) and 5% CO2 in a glucose-free medium. After OGD, reoxygenation was achieved by exposing the cells to normal O2 and glucose levels. Neither MTT, an index of mitochondrial oxidative phosphorylation, nor malondialdehyde (MDA) production, a parameter measuring lipid peroxidation, were affected by 1 hr of OGD in cortical neurons. When OGD was followed by 24 hr of reoxygenation, MTT levels were reduced by 40% and MDA was significantly increased, whereas cellular ATP content did not change. Cerebellar granule cells, on the other hand, did not show any reduction of mitochondrial activity after exposure to 1 hr OGD or to 1 hr OGD plus 24 hr of reoxygenation. When OGD was prolonged for 2 hr, a significant reduction of the mitochondrial activity and of cellular ATP content occurred, coupled to a significant MDA increase in cerebellar granule cells, whereas in cortical neurons a reduction of MTT levels after 2 hr OGD was not accompanied by a decrease of cellular ATP content nor by an increase of MDA production. Moreover, 24 hr of reoxygenation further reinforced lipid peroxidation, LDH release, propidium iodide positive neurons and the reduction of ATP content in cerebellar granule cells. The results of the present study collectively show that cortical and cerebellar neurons display different levels of vulnerability to reoxygenation followed by OGD. Furthermore, the impairment of mitochondrial activity and the consequent overproduction of free radicals in neurons were observed for the first time occurring not only during the reoxygenation phase, but already beginning during the OGD phase.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Scorziello A,Pellegrini C,Forte L,Tortiglione A,Gioielli A,Iossa S,Amoroso S,Tufano R,Di Renzo G,Annunziato Ldoi
10.1002/1097-4547(20010101)63:1<20::AID-JNR3>3.0.Ckeywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
20-6issue
1eissn
0360-4012issn
1097-4547pii
10.1002/1097-4547(20010101)63:1<20::AID-JNR3>3.0.Cjournal_volume
63pub_type
杂志文章abstract::Although reactive oxygen species (ROS) have been implicated in ischemic preconditioning (IPC)-induced neuronal protection, several key questions concerning ROS remain to be elucidated. The purpose of this study is to obtain direct evidence for the formation of specific ROS species generated by IPC, and to determine th...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20402
更新日期:2005-03-15 00:00:00
abstract::Previous studies have suggested that sensory mechanisms may be important components of addiction to, and withdrawal from, cigarette smoking. The sensory and respiratory responses to nicotine are mediated, in part, by bronchopulmonary C-fiber afferents. Nicotine has a direct stimulatory effect on pulmonary sensory neur...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21967
更新日期:2009-05-15 00:00:00
abstract::Transient global ischemia causes delayed white matter injury to the brain with oligodendrocyte (OLG) death and myelin breakdown. There is increasing evidence that hypoxia may be involved in several diseases of the white matter, including multiple sclerosis, vascular dementia, and ischemia. Matrix metalloproteinases (M...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22257
更新日期:2010-03-01 00:00:00
abstract::Astrocytes purified from the neonatal rat brain were cultured for up to 3 weeks prior to being treated with agents that can induce reactive changes in astrocytes. These agents included dibutyryl cAMP, interleukin-1 beta, and macrophage conditioned media. After treating astrocytes for 3 days, the agents were removed an...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490380108
更新日期:1994-05-01 00:00:00
abstract::The applications of neural progenitor cells in clinical therapy for neural degeneration, such as Parkinson's disease, Huntington's disease, and cerebral infarction, have long been explored widely. It had been suggested that these cells may block the apoptosis of ischemia-induced neuronal damage and may themselves resi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10476
更新日期:2003-01-15 00:00:00
abstract::The normal innervation of structures in mouse foot pads was investigated with immunohistochemistry and confocal microscopy. Nerves were visualized by incubating Zamboni fixed, thick, frozen sections with antibodies to protein gene product 9.5 (PGP 9.5), vasoactive intestinal peptide, substance P, calcitonin gene-relat...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490410113
更新日期:1995-05-01 00:00:00
abstract::Deletion of the tumor suppressor gene p53 has been shown to improve the outcome in experimental models of focal cerebral ischemia and kainate-induced seizures. To evaluate the potential role of p53 in traumatic brain injury, genetically modified mice lacking a functional p53 gene (p53(-/-), n = 9) and their wild-type ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22491
更新日期:2010-11-15 00:00:00
abstract::We report a study of the characterization of voltage-activated potassium (K+) currents in retinal ON bipolar cells in zebrafish. At single-channels levels, the open probability of the K+ channels increased when the membrane potential was increased. The maximal open proportion was 0.76+/-0.05 under our testing conditio...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20637
更新日期:2005-11-01 00:00:00
abstract::As aging population is increasing, new methodologies to apprehend and enhance the mechanisms related to optimal brain function in advancing age become urgent. This review describes how the combined use of non-invasive brain stimulation (NIBS) with functional magnetic resonance imaging (fMRI) provides novel experimenta...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24514
更新日期:2019-08-16 00:00:00
abstract::Both IgCAMs and the actin cytoskeleton play critical roles in neuronal growth cone motility and guidance. However, it is unclear how IgCAM receptors transduce signals from the plasma membrane to induce actin remodeling. Previous studies have shown that local clustering and immobilization of apCAM, the Aplysia homolog ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21937
更新日期:2009-04-01 00:00:00
abstract::Vascular smooth muscle (VSM) cells comprise one of the primary targets of the sympathetic nervous system and have been shown to secrete nerve growth factor (NGF). There is increasing evidence that changes in the levels of NGF in the adult may underlie certain pathological conditions. To investigate the potential role ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract::With the use of an antiserum directed against the human smooth muscle-associated protein 8 (SMAP8) fragment SMAP8(98-138), Western blot and immunohistochemical studies revealed SMAP8 expression in the rat brain. A band with a molecular size of about 45 kDa was detected in tissues from the rat hypothalamus and a weaker...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21315
更新日期:2007-06-01 00:00:00
abstract::The ligand-activated transcription factor peroxisome proliferator-activated receptor beta (PPARbeta) is present in the brain and is implicated in the regulation of genes with potential roles in neurotoxicity. We sought to examine the role of PPARbeta in neuronal cell death by using the PPARbeta ligand GW0742. Primary ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20153
更新日期:2004-07-15 00:00:00
abstract::The presence of acetylcholinesterase has been detected in the thymus of several species both biochemically and histochemically. In this study we have investigated the molecular forms and the level of this enzyme in separate compartments of the murine thymus and in different thymocyte subpopulations. Similar levels of ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490290210
更新日期:1991-06-01 00:00:00
abstract::Molecular mechanisms which underlie the heat shock response have commonly been analyzed using tissue culture systems, with less investigation of the intact mammal. In tissue culture, a temperature elevation of 5 degrees C is required to activate mammalian heat shock transcription factor 1 (HSF1) to the DNA-binding for...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19960401)44:1<52::AID-JNR7
更新日期:1996-04-01 00:00:00
abstract::In contrast to mammals, teleost fish are able to regrow severed long-range projection axons in the central nervous system (CNS), leading to recovery of function. The optic projection in teleost fish is used to study neuron-intrinsic and environmental molecular factors that determine successful axon regrowth and naviga...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.21121
更新日期:2007-09-01 00:00:00
abstract::Oligodendrocyte (OLG) death plays a major role in white matter dysfunction and demyelination following injury to the CNS. Axonal contact, communication, and neuronal activity appear to promote OLG survival and function in cell culture and during development. The application of electrical stimulation to mixed neural cu...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22717
更新日期:2012-01-01 00:00:00
abstract::Acrylamide (ACR) is an environmental toxicant and prototypic tool for studying mechanisms of peripheral neuropathies. Reductions in fast anterograde axonal transport (faAXT) are thought to be a critical step leading to axonal degeneration. Kinesin and microtubules (MT) were evaluated as molecular sites of action using...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19961001)46:1<7::AID-JNR2>
更新日期:1996-10-01 00:00:00
abstract::A study was conducted on the adsorption of 14C-ATP to a surface film of a hydrophobic protein derived from synaptic membranes isolated from bovine cerebellum. The adsorption of ATP to the protein film followed a rate law based on diffusion and an energy barrier to adsorption, the rate law being generally applicable to...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490020109
更新日期:1976-01-01 00:00:00
abstract::Muscarinic cholinergic receptors of the M5 subtype are expressed by dopamine-containing neurons of the ventral tegmentum. These M5 receptors modulate the activity of midbrain dopaminergic neurons, which play an important role in mediating reinforcing properties of abused psychostimulants like cocaine. The potential ro...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10728
更新日期:2003-10-01 00:00:00
abstract::N-cadherin and beta-catenin are involved in cell adhesion and cell cycle in tumor cells and neural crest. Both are expressed at key stages of Schwann cell (SC) development, but little is known about their function in the SC lineage. We studied the role of these molecules in adult rat derived SC-embryonic dorsal root g...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21528
更新日期:2008-03-01 00:00:00
abstract::Numerical synaptic density and synaptic vesicle density in rat frontal cortex were examined by electron microscopy as a function of age. The density of axospinous synapses, a major population of synapses, was found to peak at age 1 month, and to gradually decrease with aging. The synaptic vesicle density in axospinous...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19990501)56:3<307::AID-JNR
更新日期:1999-05-01 00:00:00
abstract::The inner ear of humans and experimental animals demonstrate an abundance of glucocorticoid receptors (GR). Glucocorticoids (GC) are widely used to treat different hearing disorders; yet the mechanisms of GC action on the inner ear are unknown. We demonstrate how GR can directly modulate hearing sensitivity in respons...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20795
更新日期:2006-05-01 00:00:00
abstract::Neurogenesis of heterotopic gray matter in the brain of the microcephalic mouse prenatally exposed to X-rays at embryonic day 13 (E13) was studied immunohistochemically. Bromodeoxyuridine (BrdU) as a marker to label the migrating position of neuroblasts generated at various embryonic stages showed that no "inside-out"...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10018
更新日期:2001-12-15 00:00:00
abstract::CD9 is a tetra-membrane-spanning glycoprotein involved in cell adhesion, migration, and proliferation in both the immune and the immature nervous system. In this study, CD9 expression was detected in myelin of mouse brain, starting at postnatal day 16. The amount of CD9 protein continuously increased with age and pers...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19971015)50:2<312::AID-JNR
更新日期:1997-10-15 00:00:00
abstract::Damage to the lateral nucleus of the septum in the rat resulted in atypical learning to a previously meaningless stimulus. Nonreinforced preexposure to a stimulus normally makes more difficult subsequent use of that stimulus for learning. The effect of this preexposure on learning is termed latent inhibition and was d...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490040309
更新日期:1979-01-01 00:00:00
abstract::Hepatocyte growth factor (HGF) is a potent mitogen for mature hepatocytes, and it has multi-functional effects in a variety of cells in various organs. HGF stimulates DNA synthesis and promotes cell migration and morphogenesis in several cell types including the olfactory system. To characterize the potential mitogeni...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10009
更新日期:2001-11-15 00:00:00
abstract::Tumor necrosis factor-alpha (TNF-alpha), a proinflammatory cytokine, has been implicated in the pathogenesis of several disorders and injuries in the central nervous system (CNS). Unlike IGF-I, which promotes CNS growth, TNF-alpha causes brain growth retardation and neural damage. Recently TNF-alpha has been shown to ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10512
更新日期:2003-03-01 00:00:00
abstract::A novel approach was developed to raise a panel of monoclonal antibodies (mAb) against brain antigens using Xenopus oocytes as immunological vectors. Xenopus oocytes were injected to express proteins encoded by brain-derived mRNA extracted from rat cerebral cortex. A crude membrane preparation from mRNA-injected oocyt...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490290109
更新日期:1991-05-01 00:00:00
abstract::The phosphorylative neuromodulation of the regulatory subunit of protein kinase type II (R-II) in cytosolic fractions from denervated and sham-operated, contralateral soleus muscles of the rat was evaluated. The denervation-induced increase in the 32P-phosphorylation of R-II is not related to an increased dephosphoryl...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490140208
更新日期:1985-01-01 00:00:00