当前位置： SCI文献检索 > JOURNAL OF NEUROSCIENCE RESEARCH期刊下所有文献 > 4-Aminopyridine causes apoptosis and blocks an outward rectifier K+ channel in malignant astrocytoma cell lines.

4-Aminopyridine causes apoptosis and blocks an outward rectifier K+ channel in malignant astrocytoma cell lines.

Abstract：

:Among the ion channels and pumps activated by growth factor stimulation, K+ channels have been implicated in the growth and proliferation of several cancer cell lines. The role of these channels in central nervous system tumors, however, has not been described. This study used the malignant astrocytoma cell lines U87 and A172. 4-Aminopyridine (4-AP) inhibition of proliferation was dose dependent, and assessment using a TUNEL in situ assay revealed that apoptosis occurred in U87 cells with wild-type p53 but not in A172 cells with mutant p53 (24-hr incubation with mM 4-AP). In patch clamp experiments, we identified two types of K+ currents in both cell lines, a charybdotoxin-sensitive Ca2(+)-activated K+ channel and a 4-AP-sensitive outward rectifier K+ current. The outward rectifier current was blocked by 4-AP in a dose-dependent manner, with half-maximal block occurring at 3.9 mM. The blocking effect of 4 mM 4-AP was noticeable at potentials as low as -65 mV and was statistically significant at -60 mV and above, suggesting that 4-AP-sensitive current is active at physiological potentials. By contrast, charybdotoxin (1 microM) and tetraethylammonium. Cl (2 mM) blocked the Ca2(+)-activated K+ channel in both cell lines but had no appreciable effect on cell growth. Our findings reveal that 4-AP inhibits proliferation and the outward rectifier K+ channel in both U87 and A172 cells. More studies are needed, however, to describe the mechanism by which K+ channels influence proliferation and induce apoptosis.

journal_name

J Neurosci Res

journal_title

Journal of neuroscience research

authors

Chin LS,Park CC,Zitnay KM,Sinha M,DiPatri AJ Jr,Perillán P,Simard JM

subject

Has Abstract

pub_date

1997-04-15 00:00:00

pages

122-7

issue

2

eissn

0360-4012

issn

1097-4547

pii

10.1002/(SICI)1097-4547(19970415)48:2<122::AID-JNR

journal_volume

48

pub_type

杂志文章

相关文献

JOURNAL OF NEUROSCIENCE RESEARCH文献大全
  • CADASIL: extended polymorphisms and mutational analysis of the NOTCH3 gene.

    abstract：:CADASIL is a cerebrovascular disease caused by mutations in the NOTCH3 gene. Most mutations result in a gain or loss of cysteine residue in one of the 34 epidermal growth factor-like repeats in the extracellular domain of the Notch3 protein, thus sparing the number of cysteine residues. To date, more than 130 differen...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.21935

    authors： Ungaro C,Mazzei R,Conforti FL,Sprovieri T,Servillo P,Liguori M,Citrigno L,Gabriele AL,Magariello A,Patitucci A,Muglia M,Quattrone A

    更新日期：2009-04-01 00:00:00

  • Amyloid β accelerates phosphorylation of tau and neurofibrillary tangle formation in an amyloid precursor protein and tau double-transgenic mouse model.

    abstract：:In Alzheimer's disease, Aβ deposits are considered the initial cardinal events that induce tauopathy secondarily. However, the relationship between Aβ amyloidosis and tauopathy has not been determined in detail. We produced double transgenic mice, 2×TgTau(+/-) APP(+/-) , by mating Tg2576 mice that exhibit Aβ amyloidos...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.22516

    authors： Seino Y,Kawarabayashi T,Wakasaya Y,Watanabe M,Takamura A,Yamamoto-Watanabe Y,Kurata T,Abe K,Ikeda M,Westaway D,Murakami T,Hyslop PS,Matsubara E,Shoji M

    更新日期：2010-12-01 00:00:00

  • Stem cell repair of central nervous system injury.

    abstract：:Neural stem cells (NSCs) have great potential as a therapeutic tool for the repair of a number of CNS disorders. NSCs can either be isolated from embryonic and adult brain tissue or be induced from both mouse and human ES cells. These cells proliferate in vitro through many passages without losing their multipotential...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章,评审

    doi：10.1002/jnr.10240

    authors： Cao Q,Benton RL,Whittemore SR

    更新日期：2002-06-01 00:00:00

  • Effects of alcohol on brain-derived neurotrophic factor mRNA expression in discrete regions of the rat hippocampus and hypothalamus.

    abstract：:Chronic alcohol consumption has adverse effects on the central nervous system, affecting some hippocampal and hypothalamic functions. In this study we tempted to demonstrate that some of these modifications could involve impairment of neurotrophic factors. Three experimental groups of male Sprague Dawley rats were stu...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/1097-4547(20010115)63:2<200::AID-JNR1012>3

    authors： Tapia-Arancibia L,Rage F,Givalois L,Dingeon P,Arancibia S,Beaugé F

    更新日期：2001-01-15 00:00:00

  • Role for myelin-associated glycoprotein as a functional tenascin-R receptor.

    abstract：:The expression of the immunoglobulin superfamily member myelin-associated glycoprotein (MAG) and the extracellular matrix glycoprotein tenascin-R (TN-R) by oligodendrocytes overlaps in time and space. The two molecules can be neurite outgrowth-inhibitory or -promoting depending on the neuronal cell type and the enviro...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/(SICI)1097-4547(19990315)55:6<687::AID-JNR

    authors： Yang H,Xiao ZC,Becker B,Hillenbrand R,Rougon G,Schachner M

    更新日期：1999-03-15 00:00:00

  • Neurogenesis and glial proliferation are stimulated following diffuse traumatic brain injury in adult rats.

    abstract：:Although increased neurogenesis has been described in rodent models of focal traumatic brain injury (TBI), the neurogenic response occurring after diffuse TBI uncomplicated by focal injury has not been examined to date, despite the pervasiveness of this distinct type of brain injury in the TBI patient population. Here...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.22635

    authors： Bye N,Carron S,Han X,Agyapomaa D,Ng SY,Yan E,Rosenfeld JV,Morganti-Kossmann MC

    更新日期：2011-07-01 00:00:00

  • Modulation of pp60c-src tyrosine kinase activity during secretion in stimulated bovine adrenal chromaffin cells.

    abstract：:High levels of the proto-oncogene product, pp60c-src, have been found in developing and adult neural tissues as well as in certain fully mature cells of the hematopoietic lineage, e.g., platelets and myelomonocytes. Adrenal medullary chromaffin cells exhibit characteristics of both types of cells, i.e., they are deriv...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.490240107

    authors： Oddie KM,Litz JS,Balserak JC,Payne DM,Creutz CE,Parsons SJ

    更新日期：1989-09-01 00:00:00

  • Antioxidant compounds and Ca(2+) pathway blockers differentially protect against methylmercury and mercuric chloride neurotoxicity.

    abstract：:The effects of the environmental contaminants methylmercury (MeHg) and inorganic mercury (HgCl(2)) on cell viability, intracellular calcium concentration ([Ca(2+)](i)), and reactive oxygen species (ROS) generation were studied in rat cerebellar granule neuron cultures using fluorescent methods. MeHg exhibited an LC(50...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.1205

    authors： Gassó S,Cristòfol RM,Selema G,Rosa R,Rodríguez-Farré E,Sanfeliu C

    更新日期：2001-10-01 00:00:00

  • Thermal conditions during neonatal anoxia affect the endogenous level of brain-derived neurotrophic factor.

    abstract：:Anoxia during delivery is a complication that can disturb infant brain development leading to various types of neurological disorders. Our studies have shown that increased body temperature of newborn rats of both sexes intensifies the postanoxic oxidative stress and prevents triggering the endogenous adaptive respons...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.24486

    authors： Kletkiewicz H,Maliszewska J,Jaworski K,Jermacz Ł,Smoliński DJ,Rogalska J

    更新日期：2019-10-01 00:00:00

  • Excitatory amino acid neurotoxicity in cultured retinal neurons: involvement of N-methyl-D-aspartate (NMDA) and non-NMDA receptors and effect of ganglioside GM1.

    abstract：:Cultures of chicken day 8 embryo retinal cells, essentially free of contaminating non-neuronal elements, were used to examine the neurotoxicity of various excitatory amino acid transmitter receptor agonists. At 7 days in vitro, N-methyl-D-aspartate (NMDA), following 24 hr exposure to 0.1-1.0 mM, destroyed 60-70% of th...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.490270210

    authors： Facci L,Leon A,Skaper SD

    更新日期：1990-10-01 00:00:00

  • Region-specific reduction of A beta-degrading endopeptidase, neprilysin, in mouse hippocampus upon aging.

    abstract：:Metabolism of amyloid-beta peptide (A beta) is closely associated with the pathology and etiology of Alzheimer's disease (AD). Neprilysin is the only rate-limiting catabolic peptidase proven by means of reverse genetics to participate in A beta metabolism in vivo. The aim of the present study is to assess whether poss...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.10390

    authors： Iwata N,Takaki Y,Fukami S,Tsubuki S,Saido TC

    更新日期：2002-11-01 00:00:00

  • A serum-free, pyruvate-free medium that supports neonatal neural/glial cultures.

    abstract：:Tissue culture media with serum generally cause excessive astrocyte proliferation in neonatal brain cultures, and often fail to support neonatal neurons. Published serum-free media for brain cultures contain sodium pyruvate, which interferes with lactate dehydrogenase (LDH) assays for cell death. We wanted to use neon...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.490410212

    authors： Martin FC,Wiley CA

    更新日期：1995-06-01 00:00:00

  • Origin of cells in contact with the growth cones of embryonal peripheral nerves and histochemical detection of nonspecific cholinesterase activity in quail-chick and chick-quail chimeras.

    abstract：:The types of cells contacted by growth cones were examined in chick-quail and vice versa chimeras. Simultaneously, the presence of nonspecific cholinesterase (nChE) was monitored in developing peripheral nerves of these embryos. In all the chimeras studied, the growth cones were in contact with both Schwann cell proge...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.490310211

    authors： Haninec P,Dubový P

    更新日期：1992-02-01 00:00:00

  • Synthetic peptide analogs to probe the immunological expression of the rat encephalitogenic neuropeptide.

    abstract：:The rationale for designing and synthesizing 24 different peptide analogs and subpeptides of residues 69-84 of guinea pig myelin basic protein is presented. The encephalitogenic potential of 19 of these peptides in Lewis rats is also given. ...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.490180130

    authors： Hashim GA,Day ED

    更新日期：1987-01-01 00:00:00

  • Apolipoprotein E expression after spinal cord injury in the mouse.

    abstract：:Apolipoprotein E (apo-E), a protein involved in lipid metabolism and cholesterol transport, has been found to be up-regulated in CNS injury and is associated with Alzheimer's disease in humans. In this study, we show that apo-E is also up-regulated after complete spinal cord transection in the C57BL/6 mouse. In the un...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.10482

    authors： Seitz A,Kragol M,Aglow E,Showe L,Heber-Katz E

    更新日期：2003-02-01 00:00:00

  • Alterations in ciliary neurotrophic factor signaling in rapsyn deficient mice.

    abstract：:Rapsyn is a key molecule involved in the formation of postsynaptic specializations at the neuromuscular junction, in its absence there are both pre- and post-synaptic deficits including failure to cluster acetylcholine receptors. Recently we have documented increases in both nerve-muscle branching and numbers of moton...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.1109

    authors： Bartlett SE,Banks GB,Reynolds AJ,Waters MJ,Hendry IA,Noakes PG

    更新日期：2001-06-15 00:00:00

  • Expression of synaptic membrane proteins in gerbil pinealocytes in primary culture.

    abstract：:Pinealocytes of various mammalian species contain abundant synaptic-like microvesicles (SLMVs) which are considered the endocrine equivalent of neuronal synaptic vesicles. Although the pinealocytes may thus be a suitable cellular model for experimental in vitro studies of SLMVs, nothing is known about the presence of ...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/(sici)1097-4547(19970301)47:5<509::aid-jnr

    authors： Redecker P,Pabst H,Gebert A,Steinlechner S

    更新日期：1997-03-01 00:00:00

  • Ginkgolide B revamps neuroprotective role of apurinic/apyrimidinic endonuclease 1 and mitochondrial oxidative phosphorylation against Aβ25-35 -induced neurotoxicity in human neuroblastoma cells.

    abstract：:Accumulating evidence points to roles for oxidative stress, amyloid beta (Aβ), and mitochondrial dysfunction in the pathogenesis of Alzheimer's disease (AD). In neurons, the base excision repair pathway is the predominant DNA repair (BER) pathway for repairing oxidized base lesions. Apurinic/apyrimidinic endonuclease ...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.23565

    authors： Kaur N,Dhiman M,Perez-Polo JR,Mantha AK

    更新日期：2015-06-01 00:00:00

  • Chronic phencyclidine induces behavioral sensitization and apoptotic cell death in the olfactory and piriform cortex.

    abstract：:In this study, we tested the hypothesis that chronic administration of phencyclidine (PCP), an N-methyl-D-aspartate (NMDA) receptor antagonist, would cause a long-lasting behavioral sensitization associated with neuronal toxicity. Female Sprague-Dawley rats were administered PCP (20 mg/kg, i.p.) once a day for 5 days,...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/(SICI)1097-4547(19980615)52:6<709::AID-JNR

    authors： Johnson KM,Phillips M,Wang C,Kevetter GA

    更新日期：1998-06-15 00:00:00

  • Purification of the 33,000-dalton ligand binding-protein constituent of the lymphoblast substance P receptor.

    abstract：:Substance P (SP) acts as an immunoregulator by binding to specific functional cell surface receptors on a subpopulation of human T-helper lymphocytes. Receptors with similar properties have also been characterized on the human IM-9 B-lymphoblast cell line. Four distinct proteins of molecular weight (MW) 33,000, 58,000...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.490180127

    authors： McGillis JP,Organist ML,Scriven KH,Payan DG

    更新日期：1987-01-01 00:00:00

  • Mechanisms of cell death in primary cortical neurons and PC12 cells.

    abstract：:Increasing evidence suggests that the regulation of neuronal cell death is complex. In this study we compared the neurotoxic effects of tumor necrosis factor-alpha (TNFalpha), nitric oxide, and thrombin on primary rat cortical cell cultures and the neuronal PC12 cell line. Release of lactate dehydrogenase (LDH) and th...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.1119

    authors： Reimann-Philipp U,Ovase R,Weigel PH,Grammas P

    更新日期：2001-06-15 00:00:00

  • Neurovascular and neuronal protection by E64d after focal cerebral ischemia in rats.

    abstract：:Calpains and cathepsins are two families of proteases that play an important role in ischemic cell death. In this study, we investigated the effect of E64d, a mu-calpain and cathepsin B inhibitor, in the prevention of neuronal and endothelial apoptotic cell death after focal cerebral ischemia in rats. Rats underwent 2...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.20977

    authors： Tsubokawa T,Yamaguchi-Okada M,Calvert JW,Solaroglu I,Shimamura N,Yata K,Zhang JH

    更新日期：2006-09-01 00:00:00

  • Adaptation of microplate-based respirometry for hippocampal slices and analysis of respiratory capacity.

    abstract：:Multiple neurodegenerative disorders are associated with altered mitochondrial bioenergetics. Although mitochondrial O(2) consumption is frequently measured in isolated mitochondria, isolated synaptic nerve terminals (synaptosomes), or cultured cells, the absence of mature brain circuitry is a remaining limitation. He...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.22650

    authors： Schuh RA,Clerc P,Hwang H,Mehrabian Z,Bittman K,Chen H,Polster BM

    更新日期：2011-12-01 00:00:00

  • Plasmalemmal repair of severed neurites of PC12 cells requires Ca(2+) and synaptotagmin.

    abstract：:Ca(2+) and synaptotagmin (a Ca(2+)-binding protein that regulates axolemmal fusion of synaptic vesicles) play essential roles in the repair of axolemmal damage in invertebrate giant axons. We now report that neurites of a rat pheochromocytoma (PC12) cell line transected and maintained in a serum medium form a dye barr...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/1097-4547(20001115)62:4<566::AID-JNR11>3.0

    authors： Detrait ER,Yoo S,Eddleman CS,Fukuda M,Bittner GD,Fishman HM

    更新日期：2000-11-15 00:00:00

  • 1-[2-(4-Benzyloxyphenoxy)Ethyl]Imidazole inhibits monoamine oxidase B and protects against neuronal loss and behavioral impairment in rodent models of Parkinson's disease.

    abstract：:Monoamine oxidase B (MAO-B) is well known as a therapeutic target for Parkinson's disease (PD). MAO-B inhibitors retain antiparkinsonism abilities to improve motor function and prevent neuronal loss by decreasing dopamine metabolism and oxidative stress in the brain. From the study to find novel antiparkinsonism drugs...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.23577

    authors： Chung JY,Lee JW,Ryu CH,Min HK,Yoon YJ,Lim MJ,Park CH

    更新日期：2015-08-01 00:00:00

  • Forebrain-specific knockout of B-raf kinase leads to deficits in hippocampal long-term potentiation, learning, and memory.

    abstract：:Raf kinases are downstream effectors of Ras and upstream activators of the MEK-ERK cascade. Ras and MEK-ERK signaling play roles in learning and memory (L&M) and neural plasticity, but the roles of Raf kinases in L&M and plasticity are unclear. Among Raf isoforms, B-raf is preferentially expressed in the brain. To det...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.20703

    authors： Chen AP,Ohno M,Giese KP,Kühn R,Chen RL,Silva AJ

    更新日期：2006-01-01 00:00:00

  • Neuregulin-1 induces acetylcholine receptor transcription in the absence of GABPalpha phosphorylation.

    abstract：:Localization of acetylcholine receptors (AChRs) to the postsynaptic region of muscle is mediated in part by transcriptional mechanisms, because the genes encoding AChR subunits are transcribed selectively in synaptic myofiber nuclei. Neuregulin-1 (NRG-1) is a synaptic signal and induces transcription of AChRs in muscl...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.21563

    authors： Herndon CA,Fromm L

    更新日期：2008-04-01 00:00:00

  • 5-hydroxyoxindole, an indole metabolite, is present at high concentrations in brain.

    abstract：:5-Hydroxyoxindole has been identified as a urinary metabolite of indole, which is produced from tryptophane via the tryptophanase activity of gut bacteria. We have demonstrated recently that 5-hydroxyoxindole is an endogenous compound in blood and tissues of mammals, including humans. To date, 5-hydroxyoxindole's role...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.21475

    authors： Crumeyrolle-Arias M,Tournaire MC,Rabot S,Malpaux B,Thiéry JC

    更新日期：2008-01-01 00:00:00

  • Axonal dependency of the postnatal upregulation in neurofilament expression.

    abstract：:A coordinated up-regulation in the expression of all three neurofilament (NF) proteins occurs during postnatal development in the rat (Schlaepfer and Bruce, J Neurosci Res [in press], 1990a). In the present study, sciatic nerves were transected in neonatal rats in order to determine the effects of axotomy on the postn...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.490270209

    authors： Schwartz ML,Shneidman PS,Bruce J,Schlaepfer WW

    更新日期：1990-10-01 00:00:00

  • Purinergic modulation of neuronal gap junction circuits in the CNS of the leech.

    abstract：:Gap junctions (GJs) are widely distributed in brains across the animal kingdom. To visualize the GJ- coupled networks of two major mechanosensory neurons in the ganglia of medicinal leeches, we injected these cells with the GJ-permeable tracer Neurobiotin. When diffusion time was limited to only 30 min, tracer couplin...

    journal_title：Journal of neuroscience research

    pub_type： 杂志文章

    doi：10.1002/jnr.24599

    authors： Segura OM,Abdulnoor L,Hua VV,Solano MJ,Macagno ER,Baker MW

    更新日期：2020-06-01 00:00:00