Investigations on myelinogenesis in vitro: developmental expression of myelin basic protein mRNA and its regulation by thyroid hormone in primary cerebral cell cultures from embryonic mice.

Abstract:

:The concentration of myelin basic protein (MBP) mRNA in primary cultures of cells dissociated from embryonic mouse cerebra and grown in the presence of varying amounts of thyroid hormone was measured using a 32P-labeled cDNA probe and a dot-blot procedure. The cDNA probe contained 1.85 kilobases of the gene for MBP. The concentration of mRNA specific for MBP in control cells grown on a medium containing normal (euthyroid) calf serum increased with increasing age of culture. The greatest increase occurred between 15 and 35 days in culture (5.25-fold increase); whereas between 35 and 50 days in culture, the rate of accumulation slowed to yield a net increase of MBP mRNA of only 10%. The quantity of MBP mRNA was drastically diminished at all ages studied when the cells were grown from the sixth day onward on a medium containing hypothyroid calf serum. Although the amount of MBP mRNA in hypothyroid-treated cells did increase, the change in concentration was less (3.43-fold), and it peaked earlier (at 30 days). Unlike the euthyroid cells, after 30 days the MBP mRNA actually fell in the hypothyroid-treated cells. If hypothyroid media were supplemented with triiodothyronine (T3) on the eighth day in culture, the quantity of MBP mRNA in the cells was restored almost completely to the levels found in the control euthyroid cells at all ages. Therefore, the regulation of the synthesis of MBP by thyroid hormone is at least in part a pretranslational event; that is, thyroid hormone adjusts the concentration of the mRNA specific for MBP.

journal_name

J Neurosci Res

authors

Shanker G,Campagnoni AT,Pieringer RA

doi

10.1002/jnr.490170304

subject

Has Abstract

pub_date

1987-01-01 00:00:00

pages

220-4

issue

3

eissn

0360-4012

issn

1097-4547

journal_volume

17

pub_type

杂志文章
  • Tumor necrosis factor-alpha regulation of insulin-like growth factor-I, type 1 IGF receptor, and IGF binding protein expression in cerebellum of transgenic mice.

    abstract::Tumor necrosis factor-alpha (TNF-alpha), a proinflammatory cytokine, has been implicated in the pathogenesis of several disorders and injuries in the central nervous system (CNS). Unlike IGF-I, which promotes CNS growth, TNF-alpha causes brain growth retardation and neural damage. Recently TNF-alpha has been shown to ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.10512

    authors: Ye P,Price W,Kassiotis G,Kollias G,D'Ercole AJ

    更新日期:2003-03-01 00:00:00

  • Combining non-invasive brain stimulation with functional magnetic resonance imaging to investigate the neural substrates of cognitive aging.

    abstract::As aging population is increasing, new methodologies to apprehend and enhance the mechanisms related to optimal brain function in advancing age become urgent. This review describes how the combined use of non-invasive brain stimulation (NIBS) with functional magnetic resonance imaging (fMRI) provides novel experimenta...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审

    doi:10.1002/jnr.24514

    authors: Abellaneda-Pérez K,Vaqué-Alcázar L,Solé-Padullés C,Bartrés-Faz D

    更新日期:2019-08-16 00:00:00

  • Altered expression of CHL1 by glial cells in response to optic nerve injury and intravitreal application of fibroblast growth factor-2.

    abstract::The close homologue of L1 (CHL1) is a member of the L1 family of cell recognition molecules. The protein is expressed by a variety of nerve cell types and subpopulations of glial cells in vivo and promotes elongation of neurites and survival of nerve cells in vitro. Here we demonstrate that glial cells up-regulate exp...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.10533

    authors: Rolf B,Lang D,Hillenbrand R,Richter M,Schachner M,Bartsch U

    更新日期:2003-03-15 00:00:00

  • Sensory neurite outgrowth on white matter astrocytes is influenced by intracellular and extracellular S100A4 protein.

    abstract::The central nervous system (CNS) is considered a nonpermissive environment for axonal regeneration because of the presence of myelin and associated repulsive molecules. However, neural cells transplanted to the CNS preferably migrate and extend their fibers in white matter areas. We previously showed that white matter...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.20743

    authors: Fang Z,Forslund N,Takenaga K,Lukanidin E,Kozlova EN

    更新日期:2006-03-01 00:00:00

  • Comparative study of cell culture and purification methods to obtain highly enriched cultures of proliferating adult rat Schwann cells.

    abstract::We present here a fast protocol that could be used to obtain highly purified cultures of maximal proliferating adult rat Schwann cells. These adult rat Schwann cells can be transfected in a nonbiological way using the physical transfection method of electroporation. Schwann cells are decisive in recovery of peripheral...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.20166

    authors: Mauritz C,Grothe C,Haastert K

    更新日期:2004-08-01 00:00:00

  • TGF-β1 enhances the activity of acid-sensing ion channel in rat primary sensory neurons.

    abstract::Transforming growth factor-β1 (TGF-β1) is an important member of multifunctional growth factor superfamily. It has been implicated in pain signaling, but little is known about the underlying mechanisms. Herein, we report that TGF-β1 can exert a sustained enhancing effect on the functional activity of acid-sensing ion ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.24481

    authors: Qiu CY,Liu TT,Wei S,Zhou YM,Wu L,Jin Y,Hu WP

    更新日期:2019-10-01 00:00:00

  • NMDA receptor-mediated cGMP synthesis in primary cultures of mouse cerebellar granule cells appears to involve neuron-astrocyte communication with NO operating as the intercellular messenger.

    abstract::The possibility that neuron-astrocyte communication may be responsible for glutamate (Glu)-stimulated cGMP formation even in relatively homogeneous primary cultures of mouse cerebellar granule cells (7 days in vitro) was investigated. Pharmacological analysis using selective excitatory amino acid (EAA) receptor antago...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4547(19960715)45:2<129::AID-JNR

    authors: Malcolm C,Grieve A,Ritchie L,Schousboe A,Griffiths R

    更新日期:1996-07-15 00:00:00

  • Synaptosomal plasma membrane transport of excitatory sulphur amino acid transmitter candidates: kinetic characterisation and analysis of carrier specificity.

    abstract::The transport kinetics of the excitatory sulphur-containing amino acid (SAA) transmitter candidates, L-cysteine sulphinate (L-CSA), L-cysteate (L-CA), L-homocysteine sulphinate (L-HCSA), and L-homocysteate (L-HCA), together with their plasma membrane carrier specificity, was studied in cerebrocortical synaptosome frac...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490320108

    authors: Grieve A,Butcher SP,Griffiths R

    更新日期:1992-05-01 00:00:00

  • Membrane protein and glycoprotein composition of beef brain synaptic vesicles.

    abstract::Synaptic vesicles were isolated from adult bovine cortical gray matter by differential centrifugation and membrane filtration of a hypoosmotically lysed crude mitochondrial fraction. Vesicle preparations were analyzed for purity by electron microscopy and enzyme assays. Polyacrylamide gel electrophoresis of SDS-solubi...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490030204

    authors: Richter-Landsberg C,Neuhoff V,Waehneldt TV

    更新日期:1977-01-01 00:00:00

  • ADAMs family members as amyloid precursor protein alpha-secretases.

    abstract::In the non-amyloidogenic pathway, the Alzheimer's amyloid precursor protein (APP) is cleaved within the amyloid-beta domain by alpha-secretase precluding deposition of intact amyloid-beta peptide. The large ectodomain released from the cell surface by the action of alpha-secretase has several neuroprotective propertie...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审

    doi:10.1002/jnr.10737

    authors: Allinson TM,Parkin ET,Turner AJ,Hooper NM

    更新日期:2003-11-01 00:00:00

  • Increase in cathepsin D activity in rat brain in aging.

    abstract::Cathepsin D-like activity in homogenates of five brain areas of 3-month-old and 24-month-old Fischer 344 rats was measured. With hemoglobin as substrate at pH 3.2, more than 90% of the activity was inhibited by pepstatin. In each area studied, activity was more than twice as high in the old rat brain: 140-160% higher ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490230412

    authors: Kenessey A,Banay-Schwartz M,DeGuzman T,Lajtha A

    更新日期:1989-08-01 00:00:00

  • Inflammatory markers in women with postpartum depressive symptoms.

    abstract::Postpartum depression (PPD) is a devastating disorder affecting not only more than 10% of all women giving birth, but also the baby, the family, and the society. Compiling evidence suggests the involvement of the immune system in the pathophysiology of major depression; yet, the immune response in perinatal depression...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.24312

    authors: Bränn E,Fransson E,White RA,Papadopoulos FC,Edvinsson Å,Kamali-Moghaddam M,Cunningham JL,Sundström-Poromaa I,Skalkidou A

    更新日期:2020-07-01 00:00:00

  • Projection target-specific action of nicotine in the caudal nucleus of the solitary tract.

    abstract::The brainstem nucleus of the solitary tract (NTS) is the key integrating relay in the central processing of sensory information from the thoracic and from most subdiaphragmatic viscera. Modulation of neuronal excitability and synaptic activity in the NTS by nicotinic agents can have potent effects on vital physiologic...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.23436

    authors: Feng L,Uteshev VV

    更新日期:2014-11-01 00:00:00

  • Time-restricted feeding prevents depressive-like and anxiety-like behaviors in male rats exposed to an experimental model of shift-work.

    abstract::Individuals who regularly shift their sleep timing, like night and/or shift-workers suffer from circadian desynchrony and are at risk of developing cardiometabolic diseases and cancer. Also, shift-work is are suggested to be a risk factor for the development of mood disorders such as the burn out syndrome, anxiety, an...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.24741

    authors: Guerrero-Vargas NN,Zárate-Mozo C,Guzmán-Ruiz MA,Cárdenas-Rivera A,Escobar C

    更新日期:2021-02-01 00:00:00

  • Lovastatin treatment decreases mononuclear cell infiltration into the CNS of Lewis rats with experimental allergic encephalomyelitis.

    abstract::Mononuclear cell infiltration into the CNS and induction of inflammatory cytokines and iNOS in diseases like multiple sclerosis (MS) and experimental allergic encephalomyelitis (EAE) have been implicated in subsequent disease pathogenesis and progression. We report that Lovastatin treatment blocks the clinical disease...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.1207

    authors: Stanislaus R,Singh AK,Singh I

    更新日期:2001-10-15 00:00:00

  • Differentiation of PC12 cells with K-ras: comparison with nerve growth factor.

    abstract::The cell line PC12, derived from a rat pheochromocytoma, has served as a model for studies on the mechanism of action of nerve growth factor, as well as for the exploration of neuronal differentiation in general. When treated with nanomolar concentrations of nerve growth factor, these neoplastic chromaffin-like cells ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490280405

    authors: Simpson DL,Dickens G,Doll S,Koizumi S,Tocco M,Okuda O,Oshima M,Rudkin BB,Brightman M,Guroff G

    更新日期:1991-04-01 00:00:00

  • Quercetin improves the activity of the ubiquitin-proteasomal system in 150Q mutated huntingtin-expressing cells but exerts detrimental effects on neuronal survivability.

    abstract::Quercetin, a strong free radical scavenger, is investigated for neuroprotective effects in a Neuro 2a cell line conditionally transfected with 16Q huntingtin (Htt) and 150Q Htt, which express the protein upon stimulation. Cells were protected from death by a 20-µM dose of quercetin on the second day of Htt induction, ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.23618

    authors: Chakraborty J,Rajamma U,Jana N,Mohanakumar KP

    更新日期:2015-10-01 00:00:00

  • Inhibition of N-cadherin and beta-catenin function reduces axon-induced Schwann cell proliferation.

    abstract::N-cadherin and beta-catenin are involved in cell adhesion and cell cycle in tumor cells and neural crest. Both are expressed at key stages of Schwann cell (SC) development, but little is known about their function in the SC lineage. We studied the role of these molecules in adult rat derived SC-embryonic dorsal root g...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.21528

    authors: Gess B,Halfter H,Kleffner I,Monje P,Athauda G,Wood PM,Young P,Wanner IB

    更新日期:2008-03-01 00:00:00

  • Pituitary adenylate cyclase-activating polypeptide counteracts the impaired adult neural stem cell viability induced by palmitate.

    abstract::Diabetes and obesity are characterized by hyperlipidemia and represent risk factors for premature neurological disorders. Diabetic/obese animals have impaired adult neurogenesis. We hypothesize that lipotoxicity leading to neurogenesis impairment plays a role in the development of neurological complications. If so, no...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.22803

    authors: Mansouri S,Ortsäter H,Pintor Gallego O,Darsalia V,Sjöholm A,Patrone C

    更新日期:2012-04-01 00:00:00

  • Effect of rosmarinic acid in motor dysfunction and life span in a mouse model of familial amyotrophic lateral sclerosis.

    abstract::Amyotrophic lateral sclerosis (ALS) is a late-onset progressive neurodegenerative disease affecting motor neurons. About 2% of patients with the disease are associated with mutations in the gene encoding Cu/Zn superoxide dismutase (SOD1). The purpose of this study is to assess the effect of rosemary extract and its ma...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.22242

    authors: Shimojo Y,Kosaka K,Noda Y,Shimizu T,Shirasawa T

    更新日期:2010-03-01 00:00:00

  • Establishment of graded spinal cord injury model in a nonhuman primate: the common marmoset.

    abstract::Most previous studies on spinal cord injury (SCI) have used rodent models. Direct extrapolation of the results obtained in rodents to clinical cases is difficult, however, because of neurofunctional and anatomic differences between rodents and primates. In the present study, the development of histopathologic changes ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.20435

    authors: Iwanami A,Yamane J,Katoh H,Nakamura M,Momoshima S,Ishii H,Tanioka Y,Tamaoki N,Nomura T,Toyama Y,Okano H

    更新日期:2005-04-15 00:00:00

  • P2 receptors, microglial cytokines and chemokines, and neuropathic pain.

    abstract::Neuropathic pain is a debilitating chronic pain and represents a major clinical challenge. The molecular and cellular mechanisms underlying its development and maintenance are not fully understood but involve abnormal excitability in the dorsal horn of the spinal cord. A growing body of evidence has shown that this ab...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审

    doi:10.1002/jnr.23816

    authors: Tsuda M

    更新日期:2017-06-01 00:00:00

  • Differential lineage restriction of rat retinal progenitor cells in vitro and in vivo.

    abstract::To identify and characterize the lineage potential of rat neural retina progenitor cells (NRPCs) in vitro and engrafted into rats with retinal degeneration, NRPCs were isolated from neural retinas of embryonic day 17 Long Evans rats and cultured in serum-free or serum-containing media with fibroblast growth factor 2 a...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.10320

    authors: Yang P,Seiler MJ,Aramant RB,Whittemore SR

    更新日期:2002-08-15 00:00:00

  • Transgenic mice expressing yellow fluorescent protein under control of the human tyrosine hydroxylase promoter.

    abstract::Pathogenesis of Parkinson's disease and related catecholaminergic neurological disorders is closely associated with changes in the levels of tyrosine hydroxylase (TH). Therefore, investigation of the regulation of the TH gene system should assist in understanding the pathomechanisms involved in these neurological diso...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.23085

    authors: Choi EY,Yang JW,Park MS,Sun W,Kim H,Kim SU,Lee MA

    更新日期:2012-10-01 00:00:00

  • Age-related changes in rat brain monoamines release: peculiarity of dopamine release.

    abstract::Our aim has been to investigate the ability of the rat brain to retain its level of neurotransmitter release over life. We have investigated the neurotransmitter release from the rat brain synaptosomes prelabeled with 3H-DA, 3H-NA, or 3H-5HT, and perfused with Krebs-Ringer medium alone (basal release) or containing a ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490340314

    authors: Cerrito F,Aloisi G,Arminio P

    更新日期:1993-02-15 00:00:00

  • Myelin-associated glycoprotein and its axonal receptors.

    abstract::Myelin-associated glycoprotein (MAG) is expressed on the innermost myelin membrane wrap, directly apposed to the axon surface. Although it is not required for myelination, MAG enhances long-term axon-myelin stability, helps to structure nodes of Ranvier, and regulates the axon cytoskeleton. In addition to its role in ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审

    doi:10.1002/jnr.21992

    authors: Schnaar RL,Lopez PH

    更新日期:2009-11-15 00:00:00

  • Regulation of pp60(c-src) synthesis in rat hippocampal slices by in vitro ischemia and glucocorticoid administration.

    abstract::Corticosteroids, released from the adrenal gland in response to stress, bind to receptors that act as transcription factors to alter gene expression and, subsequently, protein synthesis. Using [(35)S]-methionine-cysteine incorporation to measure protein synthesis in hippocampal slices incubated under ischemic conditio...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.1159

    authors: Barr CS,Dokas LA

    更新日期:2001-08-15 00:00:00

  • Expression of an engrailed-like gene during development of the early embryonic chick nervous system.

    abstract::The engrailed gene has been identified in Drosophila as an important developmental gene involved in the control of segmentation. Here we describe the embryonic expression of a chicken gene, ChickEn (Darnell et al.: J Cell Biol 103(5):311a, 1986), which contains homology to the Drosophila engrailed gene. Northern blots...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490210234

    authors: Gardner CA,Darnell DK,Poole SJ,Ordahl CP,Barald KF

    更新日期:1988-10-01 00:00:00

  • Protective effect of L-trans-pyrrolidine-2,4-dicarboxilic acid preload against cell death induced by oxygen/glucose deprivation in differentiated PC12 cells.

    abstract::It has been postulated that cellular glutamate is released into the extracellular fluid when the energy supply of the brain is compromised (i.e., anoxia or oxygen/glucose deprivation), and there the amino acid triggers the so-called excitotoxic cascade, causing neuronal death. Several mechanisms for this release have ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.20613

    authors: Martín ME,Muñoz FM,Dickinson DA,Forman HJ,Martín del Río R,Salinas M,Fando JL

    更新日期:2005-10-01 00:00:00

  • Mouse sciatic nerve regeneration through semipermeable tubes: a quantitative model.

    abstract::The regeneration of transected mouse sciatic nerves using semipermeable acrylic copolymer tubes to enclose both stumps has been qualitatively assessed from 1 to 30 weeks post-operative. Quantitative morphometric analysis of electron micrograph montages of complete transverse sections of the segment regenerated between...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490090309

    authors: Uzman BG,Villegas GM

    更新日期:1983-01-01 00:00:00