Abstract:
:A distinct population of white matter progenitor cells (WMPCs), competent but not committed to generate oligodendrocytes, remains ubiquitous in the adult human subcortical white matter. These cells are present in both sexes and into senescence and may constitute as much as 4% of the cells of adult human capsular white matter. Transduction of adult human white matter dissociates with plasmids bearing early oligodendrocytic promoters driving fluorescent reporters permits the separation of these cells at high yield and purity, as does separation based on their expression of A2B5 immunoreactivity. Isolates of these cells survive xenograft to lysolecithin-demyelinated brain and migrate rapidly to infiltrate these lesions, without extending into normal white matter. Within several weeks, implanted progenitors mature as oligodendrocytes, and develop myelin-associated antigens. Lentiviral tagging with green fluorescent protein confirmed that A2B5-sorted progenitors develop myelin basic protein expression within regions of demyelination and that they fail to migrate when implanted into normal brain. Adult human white matter progenitor cells can thus disperse widely through regions of experimental demyelination and are able to differentiate as myelinating oligodendrocytes. This being the case, they may constitute appropriate vectors for cell-based remyelination strategies.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Windrem MS,Roy NS,Wang J,Nunes M,Benraiss A,Goodman R,McKhann GM 2nd,Goldman SAdoi
10.1002/jnr.10397keywords:
subject
Has Abstractpub_date
2002-09-15 00:00:00pages
966-75issue
6eissn
0360-4012issn
1097-4547journal_volume
69pub_type
杂志文章abstract::Brain metabolism increases during stimulation, but this increase does not affect all energy metabolism equally. Briefly after stimulation, there is a local increase in cerebral blood flow and in glucose uptake, but a smaller increase in oxygen uptake. This indicates that temporarily the rate of glycolysis is faster th...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24374
更新日期:2019-08-01 00:00:00
abstract::The regeneration of transected mouse sciatic nerves using semipermeable acrylic copolymer tubes to enclose both stumps has been qualitatively assessed from 1 to 30 weeks post-operative. Quantitative morphometric analysis of electron micrograph montages of complete transverse sections of the segment regenerated between...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490090309
更新日期:1983-01-01 00:00:00
abstract::Butyrate produced a biphasic modulation of the thyroid hormone receptor in neuroblastoma N2A cells increasing receptor number by 20-35% at concentrations 0.25-0.75 mM and decreasing receptor levels by 30-55% at 2-4 mM. The half-life of the receptor, as assessed by its disappearance after incubation with 18 microM cycl...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490270102
更新日期:1990-09-01 00:00:00
abstract::In offspring, an adequate maternal diet is important for neurodevelopment. One mechanism by which maternal diet impacts neurodevelopment is through its dynamic role in the development of the gut microbiota. Communication between the gut, and its associated microbiota, and the brain is facilitated by the vagus nerve, i...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24605
更新日期:2021-01-01 00:00:00
abstract::A series of major and minor ganglioside species were isolated from a single human adult brain and analyzed for their lipophilic composition. Hematosides, GM3 and GD3, each showing double bands on thin-layer chromatograms, were separated into the upper band and lower band fractions to be analyzed for the heterogeneity ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490120208
更新日期:1984-01-01 00:00:00
abstract::Light microscopic anterograde tracing studies indicate that neurons in the central nucleus of the amygdala (CNA) project to a region of the dorsal pontine tegmentum ventral to the superior cerebellar peduncle which contains noradrenergic dendrites of the nucleus locus coeruleus (LC). However, it has not been establish...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19960801)45:3<289::AID-JNR
更新日期:1996-08-01 00:00:00
abstract::Perinatal hypoxia is a major cause of neurodevelopmental deficits. Neuronal migration patterns are particularly sensitive to perinatal hypoxia/ischemia and are associated with the clinical deficits. The rat model of hypoxia/ischemia at P7 mimics that of perinatal injury in humans. Before assessing the effects of postn...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21607
更新日期:2008-05-15 00:00:00
abstract::Understanding the pathway for amyloid percursor protein (APP) catabolism has become an important line of investigation. APP is a ubiquitous membrane bound protein that is rapidly cleaved at the membrane, yielding a secreted protein identical to protease nexin II and an internalized 11.5 kDa 100 residue C terminal deri...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490330121
更新日期:1992-09-01 00:00:00
abstract::Isatin (indole-2,3-dione) is an endogenous indole that has a distinct and discontinuous distribution in the brain and in other mammalian tissues and body fluids. Its output is increased under conditions of stress and anxiety. Its biological targets remain poorly characterized, although [(3)H]isatin binding sites have ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22104
更新日期:2009-09-01 00:00:00
abstract::Neuroadaptations in the nucleus accumbens (NAc) are associated with the development of drug addiction. Plasticity in synaptic strength and intrinsic excitability of NAc medium spiny neurons (MSNs) play critical roles in addiction induced by different classes of abused drugs. However, it is unknown whether morphine exp...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23025
更新日期:2012-06-01 00:00:00
abstract::It is suggested that masticatory dysfunction affects the central nervous system; however, the underlying mechanism remains unknown. Brain-derived neurotrophic factor (BDNF) and its receptor, TrkB, are known to play important roles in memory and learning. In this study, we examined the effects of mastication on memory,...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23383
更新日期:2014-08-01 00:00:00
abstract::Neurofibromatosis type 2 is a hereditary cancer syndrome characterized by the development of bilateral vestibular schwannomas. Underlying the disease are inactivating mutations of the NF2 tumor suppressor gene, located on chromosome 22, encoding a 595-amino-acid protein. The NF2 protein, also known as merlin or schwan...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/1097-4547(20001215)62:6<764::AID-JNR2>3.0.
更新日期:2000-12-15 00:00:00
abstract::Although both cytokines and chemokines have been implicated in the pathogenesis of clinical and histological EAE, their interactions in vivo have not yet been clearly established. To address this issue, we evaluated expression of chemokines and receptors in the CNS of wild-type control and cytokine deficient mice at t...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10156
更新日期:2002-03-01 00:00:00
abstract::Macroautophagy is an evolutionally conserved membrane trafficking pathway that delivers intracellular materials to lysosomes for degradation and recycling. Rab7, as a member of the Rab GTPase superfamily, has a unique role in the regulation of macroautophagy, especially in modulating autophagy flux. The functional sta...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24034
更新日期:2017-10-01 00:00:00
abstract::The chemokines, macrophage inflammatory protein-1 (MIP-1) and its subunit MIP-1 beta, induce an intense fever in the rat when they are injected directly into the anterior hypothalamic, pre-optic area (AH/POA), a region containing thermosensitive neurons. The purpose of this study was to compare the central action on b...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490390105
更新日期:1994-09-01 00:00:00
abstract::The limited availability and potential to culture primary human brain cells means that there is still a need for cell lines that reliably model human neurons and glial cells. The human-derived NTera2/D1 (NT2) cell line is a promising tool from which both neuronal (NT2N) and astrocytic (NT2A) cells can be derived in vi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22692
更新日期:2011-10-01 00:00:00
abstract::Structural microtubule-associated proteins (MAPs) interact with microtubules to regulate the various dynamic stages of microtubules. The purpose of this study was to measure the impact of myc-tagged MAP1a fragments on microtubule dynamic phases in vivo. Cells from an epithelial kidney cell line (LLCPK1) that had been ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21920
更新日期:2009-04-01 00:00:00
abstract::Two fundamental questions about neuron cell culture were addressed. Can one serum-free medium that was developed for optimum growth of hippocampal neurons support the growth of neurons from other regions of the brain? Is the region specific state of differentiation maintained in culture? To answer these questions, we ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490420510
更新日期:1995-12-01 00:00:00
abstract::Molecular mechanisms which underlie the heat shock response have commonly been analyzed using tissue culture systems, with less investigation of the intact mammal. In tissue culture, a temperature elevation of 5 degrees C is required to activate mammalian heat shock transcription factor 1 (HSF1) to the DNA-binding for...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19960401)44:1<52::AID-JNR7
更新日期:1996-04-01 00:00:00
abstract::Post-translational modification of neural cell adhesion molecule (NCAM) with alpha2,8-linked polysialic acid, which regulates homophilic adhesion and/or signal transduction events, is crucial to synaptic plasticity in the developing and adult brain. Evidence from in vitro models has implicated polysialylation in the r...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/1097-4547(20000915)61:6<636::AID-JNR7>3.0.
更新日期:2000-09-15 00:00:00
abstract::The olfactory ensheathing cell (OEC) is a unique glial cell able to support neurite outgrowth in the CNS throughout life. The OEC has been described as having both Schwann cell-like and astrocyte-like characteristics. The purpose of this study was to compare gap junctional communication and connexin (Cx) expression in...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.1182
更新日期:2001-09-15 00:00:00
abstract::Miniature endplate potentials (MEPPs) with slow rising phase can be attributed either to burst of transmitter releases or to distortion of conduction from remote releasing sites. The spontaneous activity of neuromuscular junctions recorded extracellularly at mouse diaphragms using sharp electrodes was analyzed to test...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490310313
更新日期:1992-03-01 00:00:00
abstract::Oxidative stress, neuroinflammation, and excitotoxicity are frequently considered distinct but common hallmarks of several neurological disorders, including Parkinson's disease, amyotrophic lateral sclerosis, multiple sclerosis, and Alzheimer's disease. Although neuron degeneration and death are the ultimate consequen...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.21325
更新日期:2007-08-01 00:00:00
abstract::Myelin gene expression in normal oligodendrocytes (OLG) depends on developmentally regulated protein kinase C (PKC) enzyme activity (Asotra and Macklin: J Neurosci Res 34:571-588, 1993). We studied the developmental expression of the Ca(++)-dependent PKC-alpha, -beta 1, -beta II and -gamma isozymes, and the Ca(++)-ind...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490390305
更新日期:1994-10-15 00:00:00
abstract::Evidence indicates that the ubiquitin-proteasome system and the endoplasmic retculum (ER) quality-control system work in concert to ensure that proteins are correctly folded in the ER and that misfolded proteins are retrotransported to the cytosol for degradation by proteasomes. Dysfunction of either system results in...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23413
更新日期:2014-10-01 00:00:00
abstract::The neuroblastoma, N1E-115, was grown for 9 days after subcultivation. The development of acetylcholinestrase (AChE), choline acetyltransferase (CAT), QNB-binding, choline uptake, and acetylcholine release was measured on days 1, 3, 6, and 9. In parallel experiments the irreversible AChE inhibitor soman was added to n...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490270115
更新日期:1990-09-01 00:00:00
abstract::The expression of the immunoglobulin superfamily member myelin-associated glycoprotein (MAG) and the extracellular matrix glycoprotein tenascin-R (TN-R) by oligodendrocytes overlaps in time and space. The two molecules can be neurite outgrowth-inhibitory or -promoting depending on the neuronal cell type and the enviro...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19990315)55:6<687::AID-JNR
更新日期:1999-03-15 00:00:00
abstract::The male preponderance in autism prevalence has brought together the disparate topics of sex/gender and autism research. Two directions of neuroimaging studies on the relationships between sex/gender and autism may inform male-specific risk mechanisms and female-specific protective mechanisms of autism. First, we revi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.23948
更新日期:2017-01-02 00:00:00
abstract::The Oct-2 transcription factor has a predominantly inhibitory effect on gene expression in neuronal cell lines. This factor and its corresponding mRNA have previously been shown to be elevated in adult rat dorsal root ganglion (DRG) neurons chronically exposed to nerve growth factor (NGF). Here we show that the Oct-2 ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490400205
更新日期:1995-02-01 00:00:00
abstract::In its severe form, X-linked adrenoleukodystrophy (ALD) is a lethal neurologic disease of children, characterized by progressive cerebral demyelination and adrenal insufficiency. Associated with a biochemical defect of peroxisomal beta-oxidation, very long-chain fatty acids (VLCFA) build up in tissues that have a high...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19971201)50:5<829::AID-JNR
更新日期:1997-12-01 00:00:00