Abstract:
:Cortical or total brain cultures of microglia are commonly used as a model to study the inflammatory processes in Parkinson's disease. Here we characterize microglia cultures from rat ventral midbrain and evaluate their response to zymosan A. We used specific markers of microglia and evaluated the morphology, the phagocytic activity and reactive oxygen species (ROS) levels of the cells. During the first 10 days in vitro (DIV), cultures presented predominantly cells with a round morphology, expressing CD68 and with high phagocytic activity and ROS production. After 13 DIV, this tendency was reversed, with cultures showing higher number of ramified cells and fewer CD68(+) cells along with lower phagocytic and ROS production capability, suggesting that microglia must be kept in vitro for at least 13 days to recover its resting state. The exposure of cultures with less than 10 DIV to zymosan A significantly decreased cell viability. Exposure of cultures with 13 DIV to zymosan A (0.05, 0.5, or 5 microg/ml) increased the total cell number, the percentage of CD68(+) cells, and the phagocytic activity. Concentrations of zymosan A higher than 5 microg/ml were also effective in activating microglia but significantly decreased the number of viable cells. In summary, microglial cells remain in the activated state for several days after the isolation process and, thus, stimulation of microglia recently isolated can compromise interpretation of the results. However, upon 13 DIV, cells achieve properties of nonactivated microglia and present a characteristic response to a proinflammatory agent.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Cristóvão AC,Saavedra A,Fonseca CP,Campos F,Duarte EP,Baltazar Gdoi
10.1002/jnr.22219subject
Has Abstractpub_date
2010-02-15 00:00:00pages
552-62issue
3eissn
0360-4012issn
1097-4547journal_volume
88pub_type
杂志文章abstract::The twitcher mouse is well known as a naturally occurring authentic mouse model of human globoid cell leukodystrophy (GLD; Krabbe disease) due to genetic deficiency of lysosomal galactosylceramidase. The twitcher mice used most commonly are on the C57BL/6J background. We generated twitcher mice that were on the mixed ...
journal_title:Journal of neuroscience research
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doi:10.1002/jnr.20190
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journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
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doi:10.1002/jnr.10061
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21272
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10116
更新日期:2002-02-01 00:00:00
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journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
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更新日期:2005-04-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
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更新日期:2012-02-01 00:00:00
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journal_title:Journal of neuroscience research
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doi:10.1002/jnr.20374
更新日期:2005-02-15 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23556
更新日期:2015-06-01 00:00:00
abstract::Lipoic acid plays a crucial role as antioxidant and metabolic component of enzymes involved in glucose metabolism of different cell types. Choline alphoscerate (α-glyceryl-phosphoryl-choline [αGPC]) is a semisynthetic derivative of phosphatidylcholines representing, among acetilcholine precursors, a cholinergic drug. ...
journal_title:Journal of neuroscience research
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doi:10.1002/jnr.23289
更新日期:2014-01-01 00:00:00
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journal_title:Journal of neuroscience research
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journal_title:Journal of neuroscience research
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pub_type: 杂志文章
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19961115)46:4<502::AID-JNR
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10457
更新日期:2003-01-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490130309
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abstract::The ED1 monoclonal antibody recognizes an antigen in lysosomal membranes of phagocytes. The expression of this antigen in cells increases during phagocytic activity. Here we describe the expression of ED1-immunoreactivity during the various stages of both acute (monophasic) and chronic relapsing experimental autoimmun...
journal_title:Journal of neuroscience research
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doi:10.1002/jnr.490380402
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21419
更新日期:2007-10-01 00:00:00
abstract::Larval acetylcholinesterase (acetylcholine acetylhydrolase) EC 3.1.3.7 of the trematode Schistosoma mansoni was characterized and purified by affinity chromatography. The enzyme was solubilized from sonicated cercarial tissue and showed a Km value of 1.83 mM and a Vmax value of 102 U/mg protein. It was characterized a...
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