Abstract:
:F5 was identified originally as an interleukin-2-regulated gene in L2 cells, a murine helper T-lymphocyte clone. In adult mouse, F5 mRNA was expressed at a modest level in lymphoid tissues, at a high level in mature neurons in the nervous system, but not in other tissues. Although the F5 sequence is highly conserved over evolution, the function of the F5 protein is unknown. In the present studies, the putative F5 protein-coding region was translated in vitro using a reticulocyte lysate system and in Escherichia coli, yielding a protein with the predicted molecular weight of 42 kDa. Polyclonal rabbit anti-F5 antibody was generated against a synthetic peptide corresponding to the C-terminus of the F5 protein. This antibody specifically recognized recombinant F5 protein. Western blot studies demonstrated a strongly-reactive 42-kDa band and a faint 39-kDa band in extracts of adult mouse brain regions, the levels of which paralleled F5 mRNA expression. Immunoperoxidase studies of adult mouse brain demonstrated F5 immunoreactivity in neuronal perikarya and dendrites but not axons. Neurons expressing the highest levels of F5 protein corresponded to those with the highest levels of F5 mRNA. Choroid plexus epithelial cells also exhibited strong reactivity localized to their basal aspect. These observations suggest that the F5 protein, expression of which appears to be regulated predominantly at the RNA level, may be involved in the maintenance of the functional or anatomic polarity of neurons and choroid plexus epithelial cells.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Arai M,Cohen JAdoi
10.1002/jnr.490360308subject
Has Abstractpub_date
1993-10-15 00:00:00pages
305-14issue
3eissn
0360-4012issn
1097-4547journal_volume
36pub_type
杂志文章abstract::Molecular cloning has introduced an unexpected, large diversity of neurotransmitter hetero- oligomeric receptors. Extensive research on the molecular structure of the γ-aminobutyric acid receptor (GABAR) has been of great significance for understanding how the nervous system works in both vertebrates and invertebrates...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23695
更新日期:2016-02-01 00:00:00
abstract::We have proposed that lactate is a "volume transmitter" in the brain and underpinned this by showing that the lactate receptor, G-protein-coupled receptor 81 (GPR81, also known as HCA1 or HCAR1), which promotes lipid storage in adipocytes, is also active in the mammalian brain. This includes the cerebral neocortex and...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.23593
更新日期:2015-07-01 00:00:00
abstract::Insulin-like growth factors, IGF-I and IGF-II, are potent regulators of oligodendrocyte development. Most of the IGF present in vivo is bound to members of a family of six high-affinity IGF-binding proteins (IGFBPs), which can either potentiate or inhibit IGF action, depending on other conditions. Additionally, serum ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19971201)50:5<721::AID-JNR
更新日期:1997-12-01 00:00:00
abstract::Antibody-producing hybridoma cells specifically bind to microgram quantities of antigen molecules adsorbed onto the surface of plastic microtiter plates. The binding of hybridoma cells to nonantigen is optimally below 5%, similar binding of non-antibody-producing cells is 4-7%, compared to the binding of the hybridoma...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490150312
更新日期:1986-01-01 00:00:00
abstract::Recent evidence has demonstrated that neural stem cells (NSC) can be expanded from a variety of sources, including embryos, fetuses, and adult bone marrow and brain tissue. We have previously reported the generation of adult rat bone marrow-derived cellular spheres that are morphologically and phenotypically similar t...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21131
更新日期:2007-02-01 00:00:00
abstract::Microarray analyses demonstrated that a variety of genes was affected by treatment of cerebellar granule cell neurons with the neurotrophic factor pigment epithelium-derived factor (PEDF). The genes for neurotrophins, glial cell-derived neurotrophic factor (GDNF), and their receptors were regulated differentially in i...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20196
更新日期:2004-09-01 00:00:00
abstract::Production of hydrogen peroxide as a by-product of the breakdown of catecholamines by the enzyme monoamine oxidase (MAO) has been hypothesized to contribute to the increased proclivity of dopaminergic neurons for oxidative injury. We established clonal dopaminergic PC12 cell lines which have elevated MAO activity leve...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19961215)46:6<666::AID-JNR
更新日期:1996-12-15 00:00:00
abstract::Preterm birth results in significant cognitive and motor disabilities, but recent evidence suggests that there is variable recovery over time. One possibility that may explain this variable recovery entails variable neurogenic responses in the subventricular zone (SVZ) following the period of chronic hypoxia experienc...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21597
更新日期:2008-05-01 00:00:00
abstract::The exposure of SY5Y neuroblastoma cells to high concentrations of glucose, fructose, or galactose is an experimental model commonly used for in vitro evaluation of typical neuronal alterations observed in diabetes mellitus. In the present study, we observed that 2 weeks of exposure to high carbohydrate concentrations...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1999-09-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) glycoprotein gp120 causes neuronal cell death; however, the molecular mechanisms of the neurotoxic effect remain largely unresolved. It has been suggested that gp120 evokes cell death by inducing the release of neurotoxins, including glutamate. The objective of this work...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10826
更新日期:2004-01-01 00:00:00
abstract::Long-duration exposure to weightlessness results in bone demineralization, muscle atrophy, cardiovascular deconditioning, altered sensory-motor control, and central nervous system reorganizations. Exercise countermeasures and body loading methods so far employed have failed to prevent these changes. A human mission to...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/1097-4547(20001015)62:2<169::AID-JNR2>3.0.
更新日期:2000-10-15 00:00:00
abstract::Extracellular matrix molecules take part in functional isolation and stabilization of neuronal compartments but form a vivid interface between neuronal elements at the same time. Previous studies have shown that the accumulation of extracellular matrix, especially its typical phenotypic form, termed perineuronal nets,...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22493
更新日期:2010-11-15 00:00:00
abstract::Nerve growth factor (NGF) is the best-characterized neurotrophic substance and has recently been shown to influence cholinergic neurons in the basal forebrain. Hippocampus and neocortex, the primary targets for these central neurons, have further been found to contain high levels of NGF. Using enzyme immunoassay and a...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490180404
更新日期:1987-01-01 00:00:00
abstract::Multi-unit activity was recorded from the diencephalon of unanesthetized, immobilized female rats. Less than 50% of the recording sites in the hypothalamus showed changes in the level of multi-unit activity to changes in the level of glucose utilization induced by injection of 2-deoxy-D-glucose (2-DG). It was also not...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490050308
更新日期:1980-01-01 00:00:00
abstract::A high density of opioid receptor-like 1 (ORL1) receptor (also referred to as NOP receptor) is found in limbic areas and in regions containing monoamines, which are implicated in emotional activity and physiopathology of depression and anxiety. We aimed at defining precisely the localization of ORL1 receptors in dorsa...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20571
更新日期:2005-08-15 00:00:00
abstract::Although alterations in adenylate cyclase (AC) activity and somatostatin (SRIF) receptor density have been reported in Alzheimer's disease, the effects of amyloid beta-peptide (Abeta) on these parameters in the hippocampus are unknown. Our aim was to investigate whether the peptide fragment Abeta(25-35) can affect the...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21115
更新日期:2007-02-01 00:00:00
abstract::TCP and its derivative gacyclidine (+/- GK11) are high-affinity non-competitive antagonists of N-methyl-D-aspartate (NMDA) receptors (NMDARs) and as such exhibit significant neuroprotective properties. These compounds also bind with a low affinity to binding sites whose pharmacological profiles are different from that...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10203
更新日期:2002-05-01 00:00:00
abstract::C6 cells were adapted to proliferate in defined culture medium to allow the study of long-term effects of serum-free growth conditions on their phenotypic antigen expression and production of neurite promoting factors (NPFs). Cultures were grown in either Ham's F-12 or supplemented Opti-MEM-I containing 15% heat-inact...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490410310
更新日期:1995-06-15 00:00:00
abstract::Studies of the effects of hypoglycemia on the brain using neurocognitive testing have suggested that mainly complex functions subserved by secondary and tertiary cortex are affected by mild to moderate hypoglycemia and that intensively treated patients with Type I diabetes mellitus (T1DM) may have altered sensitivity ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21146
更新日期:2007-02-15 00:00:00
abstract::Four brain-specific, DNase I hypersensitive sites (HSS) have been mapped to the 5' flanking region of the mouse 68-kDa neurofilament gene. These sites are contained within a 1.7-kb sequence that confers neuronal specificity of expression of this gene in transgenic mice. To identify DNA sequences that might be involved...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490320204
更新日期:1992-06-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19961001)46:1<7::AID-JNR2>
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490420103
更新日期:1995-09-01 00:00:00
abstract::Dihydropteridine reductase was purified approximately 1,700-fold from human outdated blood platelets. Two forms of the enzyme, A and B, were resolved. They have the same Km values for 2-amino-6,7,-dimethyl-4-hydroxydihydropteridine (46 microM vs 49 microM), but the A form has a Km for NADH that is two times higher tha...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490060205
更新日期:1981-01-01 00:00:00
abstract::The hippocampus is an important brain region involved in memory and learning. Brain-derived neurotrophic factor (BDNF), tyrosine kinase receptor B (TrkB), and phospho-p44/p42 mitogen-activated protein kinase (MAPK) are known to contribute to hippocampal memory/learning. The present study aimed to clarify the effects o...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.24216
更新日期:2018-06-01 00:00:00
abstract::To further our understanding of the functions of the major myelin proteins, myelin basic protein (MBP) and proteolipid protein (PLP), and other myelin proteins, such as 2'3'-cyclic nucleotide 3'-phosphodiesterase (CNP) and myelin-associated glycoprotein (MAG), bovine brain myelin was extracted with Triton X-100, and p...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10383
更新日期:2002-10-01 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is a late-onset progressive neurodegenerative disease affecting motor neurons. About 2% of patients with the disease are associated with mutations in the gene encoding Cu/Zn superoxide dismutase (SOD1). The purpose of this study is to assess the effect of rosemary extract and its ma...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22242
更新日期:2010-03-01 00:00:00
abstract::Using a canine model of classical late-infantile neuronal ceroid lipofuscinosis (CLN2 disease), a study was conducted to evaluate the potential pharmacological activity of recombinant human tripeptidyl peptidase-1 (rhTPP1) enzyme replacement therapy administered directly to the cerebrospinal fluid (CSF). CLN2 disease ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23423
更新日期:2014-11-01 00:00:00
abstract::The effects of hyposmotic conditions on taurine uptake and release were studied in mice cultured cerebellar granule cells. The effect of DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulfonate) and of the divalent cations Mg++ and Mn++ on the hyposmolarity-induced changes in these parameters was investigated. Mg++ (20 mM...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490300409
更新日期:1991-12-01 00:00:00
abstract::We generated a new cell line, N38, by conditionally immortalizing mouse oligodendrocytes (OLs) at early stages of maturation. The morphology and marker expression pattern suggest N38 cells are similar to immature OLs. N38 cells were sensitive to changes in serum concentrations, and forcing the cells to differentiate i...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/1097-4547(20001101)62:3<319::AID-JNR1>3.0.
更新日期:2000-11-01 00:00:00
abstract::Apolipoprotein E (ApoE) is the major brain lipoprotein and plays an important role in lipid transport. ApoE-deficient mice whose apoE gene has been knocked out have distinct cognitive and neurochemical deficits, and their recovery from brain injury is impaired. In the present study we examined the possibility that the...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1999-11-15 00:00:00