Abstract:
:The tissue- and cell-specific expression of three neutral glycosphingolipids, gangliotetraosylceramide (GA1), gangliopentaosylceramide (GalNAc-GA1), and the novel 3-O-acetyl-sphingosine-series glycolipid (FMC-5), were examined with monospecific polyclonal antibodies. Immunohistochemical studies of rodent brain cross-sections indicated that both GA1 and FMC-5 antibodies stained myelin. In contrast, GalNAc-GA1 antibody distinctly stained neurons in cerebral cortex, but only partially delineated Purkinje cells and other neurons in cerebellum. Preliminary studies of mixed glial cultures suggested the following: 1) both FMC-5 and GA1 antibodies stained oligodendrocytes and oligo progenitors, and 2) GalNAc-GA1 antibody did not stain any cells in the culture. Because the GalNAc-GA1 was associated with neurons, we examined the immunoreactivity of GalNAc-GA1 antibody in primary neuronal cultures. Further studies using primary cultures of rat brain oligodendrocytes, and dissociated cerebellar neuronal cultures indicated that both GA1 and FMC-5 are specifically expressed by oligodendrocytes, whereas GalNAc-GA1 is primarily localized in interneurons and to some extent in Purkinje neurons.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Dasgupta S,Bhat NR,Spicer SS,Hogan EL,Furuya S,Hirabayashi Ydoi
10.1002/jnr.21419subject
Has Abstractpub_date
2007-10-01 00:00:00pages
2856-62issue
13eissn
0360-4012issn
1097-4547journal_volume
85pub_type
杂志文章abstract::Glial cell-line derived neurotrophic factor (GDNF) is the most potent trophic factor for motoneuron survival and neuromuscular junction formation. GDNF is upregulated in injured or denervated skeletal muscle and returns to normal levels following reinnervation. However, the mechanism by which GDNF is regulated in dene...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23512
更新日期:2015-03-01 00:00:00
abstract::Increased serum level of homocysteine, a sulfur-containing amino acid, is considered a risk factor in vascular disorders and in dementias. The effect of homocysteine and metabolically related compounds on brain production of kynurenic acid (KYNA), an endogenous antagonist of glutamate ionotropic receptors, was studied...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20359
更新日期:2005-02-01 00:00:00
abstract::Neural precursor cells (NPCs) critically regulate brain morphogenesis and recent studies have revealed an unexpectedly high frequency of NPC chromosomal abnormalities and apoptosis in the developing brain. We have shown previously that the apoptotic response of NPCs to genotoxic agents is dependent on p53 and caspase-...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10738
更新日期:2003-11-01 00:00:00
abstract::Neuronal degeneration has been shown to be involved in various neurological disorders. Growth/trophic factors and their receptors are known to be important for the regeneration and survival of neurons. We report here the molecular cloning of a receptor-like protein tyrosine kinase, bsk, (for brain specific kinase). Bs...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490370117
更新日期:1994-01-01 00:00:00
abstract::The mechanisms by which polyglutamine expansion causes common features of neuronal death remain unclear. Here we describe an approach for delivering polyglutamine expansions directly into cultured sympathetic neurons. Glutamine (Q) residues (n = 10, 22, 30) were conjugated with a peptide possessing translocation prope...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20461
更新日期:2005-05-15 00:00:00
abstract::This study examined the growth of basal plate explants from 40-hr chick embryos containing the trigeminal motor nucleus in the presence of target muscle-conditioned medium (MCM) from day-4, day-10, and day-18 embryos. We had previously found that MCM derived from the 4-day target tissue enhanced neurite outgrowth from...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490190109
更新日期:1988-01-01 00:00:00
abstract::Vascular smooth muscle (VSM) cells comprise one of the primary targets of the sympathetic nervous system and have been shown to secrete nerve growth factor (NGF). There is increasing evidence that changes in the levels of NGF in the adult may underlie certain pathological conditions. To investigate the potential role ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract::Two fundamental questions about neuron cell culture were addressed. Can one serum-free medium that was developed for optimum growth of hippocampal neurons support the growth of neurons from other regions of the brain? Is the region specific state of differentiation maintained in culture? To answer these questions, we ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490420510
更新日期:1995-12-01 00:00:00
abstract::Molecular and behavioral timekeeping is regulated by the circadian system which includes the brain's suprachiasmatic nucleus (SCN) that translates environmental light information into neuronal and endocrine signals aligning peripheral tissue rhythms to the time of day. Despite the critical role of circadian rhythms in...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.24606
更新日期:2021-01-01 00:00:00
abstract::We describe an experimental model to produce closed traumatic injuries to the spinal cord of adult rats. This model uses an inflatable balloon that is introduced in the dorsal subdural space and moved to a location rostral to the laminectomy site. The spinal cord trauma can be graded by varying either the duration of ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490320409
更新日期:1992-08-01 00:00:00
abstract::The companion study showed that acoustic overstimulation of adult chinchillas, with a noise level sufficient to damage the cochlea, led to cytological changes and degeneration of synaptic endings in the cochlear nucleus within 1-16 weeks. In the present study, the same stimulus was used to study the long-term effects ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20212
更新日期:2004-09-15 00:00:00
abstract::An immortalized dorsal root ganglion cell line F-11 exhibits many properties of spinal cord neurons and undergoes apoptosis in response to growth factor withdrawal and the exogenous addition of inhibitors of phosphatidylinositol-3-kinase (PI3K). To elucidate the mechanism of apoptosis we generated F-11 clones which ov...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1999-09-15 00:00:00
abstract::Only a few proteins are known to be exclusively expressed in central nervous system (CNS) myelin. A novel surface membrane protein expressed only in CNS myelin and oligodendrocytes of higher vertebrates has been identified by a monoclonal antibody. This CNS myelin/oligodendrocyte-specific protein, MOSP, has a molecula...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490280418
更新日期:1991-04-01 00:00:00
abstract::Mitotic activity persists in various regions of the adult mammal CNS. While evidences of neurogenesis appeared, many studies focused on the features of the adult stem cells from germinative areas such as the subventricular zone of the lateral ventricles, the dentate gyrus of the hippocampus, the cortex, the fourth ven...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.20040
更新日期:2004-04-15 00:00:00
abstract::Transferrin is the second most abundant plasma protein and functions to transport iron. It is an essential constituent in culture media for virtually all cells. In a recent study, we reported that transferrin (Tf) is specifically located in oligodendrocytes in the rat nervous system. This investigation examines immuno...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490170108
更新日期:1987-01-01 00:00:00
abstract::Oligomeric forms of α-synuclein are believed to cause mitochondrial injury, which may contribute to neurotoxicity in Parkinson's disease (PD). Here oligomers of α-synuclein were prepared using the dopamine metabolite, DOPAL (3,4-dihydroxyphenyl-acetaldehyde), in the presence of guanidinium hydrochloride. Electron micr...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.24513
更新日期:2019-12-01 00:00:00
abstract::The expression of different muscarinic receptor subtypes was analyzed in immature Schwann cells obtained from sciatic nerve of 2-day neonatal rats. By using RT-PCR analysis, we demonstrated the presence of M1, M2, M3, and M4 receptor subtypes in cultured Schwann cells, with M2 displaying the highest expression levels....
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20874
更新日期:2006-07-01 00:00:00
abstract::New neurons are continuously generated throughout life in the subgranular zone in the dentate gyrus of the mammalian hippocampus and in the subventricular zone of the lateral ventricles. With the aid of new methodologies, significant progress has been made in the characterization of endogenous stem cells (ependymal ce...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.23480
更新日期:2015-03-01 00:00:00
abstract::Perceptually similar stimuli, despite not being consciously distinguishable, may result in distinct cortical brain activations. Hypothesizing that perceptually similar tastes are discriminable by electroencephalography (EEG), we recorded 22 human participants' response to equally intense sweet-tasting stimuli: caloric...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.24281
更新日期:2019-03-01 00:00:00
abstract::Nicotinates, pantothenates, riboflavin, vitamins B6 and B12' free (acetyl) and total (free and bound) choline, biopterin, thiamin, biotin, methylated and nonmethylated folates in frontal, temporal, precentral, postcentral, and occipital cortex, thalamus, cerebellum, pons, basal ganglia, and substantia nigra were estim...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490110409
更新日期:1984-01-01 00:00:00
abstract::Granulocyte colony-stimulating factor (G-CSF) is a hematopoietic cytokine that also possesses neurotrophic and antiapoptotic properties. G-CSF has been reported to decrease amyloid burden significantly, promote hippocampal neurogenesis, and improve spatial learning in a mouse model of Alzheimer's disease. To understan...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23378
更新日期:2014-08-01 00:00:00
abstract::Microarray analyses demonstrated that a variety of genes was affected by treatment of cerebellar granule cell neurons with the neurotrophic factor pigment epithelium-derived factor (PEDF). The genes for neurotrophins, glial cell-derived neurotrophic factor (GDNF), and their receptors were regulated differentially in i...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20196
更新日期:2004-09-01 00:00:00
abstract::Scatchard analysis of saturation isotherms of [125I]-iodopindolol was used to characterize beta-adrenoceptor density (Bmax) and affinity constant (Kd) in female and male hamster Harderian glands. Single-point experiments were also completed in intact females, intact males, and castrated or superior cervical ganglionec...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490220411
更新日期:1989-04-01 00:00:00
abstract::A mouse monoclonal antibody (5B9), directed against a carbohydrate epitope of human epidermal growth factor receptor (EGFR), recognized an 81-kDalton glycoprotein in buffer-soluble and detergent-solubilized rat brain extracts (BE). The glycoprotein was more abundant in extracts prepared from injured brain than in thos...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490400609
更新日期:1995-04-15 00:00:00
abstract::Thymocyte differentiation antigen-1 (Thy-1) is a cell surface glycoprotein found on T cells and neurons and is involved in cell-to-cell interactions. In addition, Thy-1 knockouts (KO) are a potential mouse model of restless legs syndrome (RLS) based on clinical observations and the role of dopamine in the disease. In ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23046
更新日期:2012-08-01 00:00:00
abstract::Brain-derived neurotrophic factor (BDNF) plays an important role in the differentiation, development, and survival of neural stem cells. In this study, we analyzed its effects on the stimulation of human umbilical cord blood-derived mesenchymal stem cells in terms of their potential to differentiate into neuron-like c...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21669
更新日期:2008-08-01 00:00:00
abstract::Brain ageing leads to atrophy and degeneration of the cholinergic nervous system, resulting in profound neurobehavioral and cognitive dysfunction from decreased acetylcholine biosynthesis and reduced secretion of growth and neurotrophic factors. Human adipose tissue-derived mesenchymal stem cells (ADMSCs) were intrave...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23182
更新日期:2013-05-01 00:00:00
abstract::Previously, we reported the presence of multiple neurotransmitters in subclones of SN6, a septal cholinergic hybrid cell line. To obtain information concerning the functionality of these transmitters, we measured transmitter contents, activities of transmitter-producing enzymes, and the effect of serum-free culture me...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490420607
更新日期:1995-12-15 00:00:00
abstract::The relationship between brain myelination and nutritional insufficiency in suckling rats whose dams had a restricted dietary intake was studied. The undernourished pups were characterized by body weight and brain weight that were 40-70% and 82-88% that of controls throughout the suckling period. Myelin concentrations...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490190311
更新日期:1988-03-01 00:00:00
abstract::Time-to-contact (TTC) perception refers to the ability of an observer to estimate the remaining time before an object reaches a point in the environment, and is of crucial importance in daily life. Noninvasive correlational approaches have identified several brain areas sensitive to TTC information. Here we report the...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.24740
更新日期:2021-02-01 00:00:00