Characterization of beta-adrenoceptors in the Syrian hamster Harderian gland: sexual differences and effects of either castration or superior cervical ganglionectomy.

Abstract:

:Scatchard analysis of saturation isotherms of [125I]-iodopindolol was used to characterize beta-adrenoceptor density (Bmax) and affinity constant (Kd) in female and male hamster Harderian glands. Single-point experiments were also completed in intact females, intact males, and castrated or superior cervical ganglionectomized males. Scatchard analysis described a single population of binding sites with a Bmax of 292.2 +/- 45.1 fmol/mg protein (X +/- SEM, n = 6) in females and 18.2 +/- 3.0 fmol/mg protein (n = 6, P less than .001) in males. The affinity also varied significantly (P less than .05) with a Kd of 1.08 +/- 0.18 versus 0.26 + 0.05 nM (n = 6) in the Harderian gland of females and males, respectively. Single-point [125I]-IPIN (400 pM) binding values in females were 67.3 +/- 4.0, in intact males were 12.8 +/- 3.2, and in castrated males were 31.2 +/- 4.2 fmol/mg protein (n = 7-9). Superior cervical ganglionectomy induced no significant changes in receptor binding. The results indicate pronounced sexual differences in the density and affinity of beta-adrenoceptors in the hamster Harderian gland, which may be sex hormone dependent.

journal_name

J Neurosci Res

authors

Pangerl A,Pangerl B,Buzzell GR,Jones DJ,Reiter RJ

doi

10.1002/jnr.490220411

subject

Has Abstract

pub_date

1989-04-01 00:00:00

pages

456-60

issue

4

eissn

0360-4012

issn

1097-4547

journal_volume

22

pub_type

杂志文章
  • 4-Aminopyridine causes apoptosis and blocks an outward rectifier K+ channel in malignant astrocytoma cell lines.

    abstract::Among the ion channels and pumps activated by growth factor stimulation, K+ channels have been implicated in the growth and proliferation of several cancer cell lines. The role of these channels in central nervous system tumors, however, has not been described. This study used the malignant astrocytoma cell lines U87 ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:

    authors: Chin LS,Park CC,Zitnay KM,Sinha M,DiPatri AJ Jr,Perillán P,Simard JM

    更新日期:1997-04-15 00:00:00

  • Somatic cell genetic analysis of the galactocerebrosidase gene: lack of complementation in human Krabbe disease/twitcher mouse cell hybrids.

    abstract::The inherited deficiency of galactosylceramide beta-galactosidase (E.C. 3.2.1.46: galactocerebrosidase) activity results in globoid cell leukodystrophy in humans (Krabbe disease) and in mice (twitcher mutant). To determine whether Krabbe patients' cells complement twitcher cells to produce, in hybrid combination, grea...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490270406

    authors: Skiba MC,Lyerla TA,Konola JT,Raghavan S

    更新日期:1990-12-01 00:00:00

  • A novel biosensor with high signal-to-noise ratio for real-time measurement of dopamine levels in vivo.

    abstract::Fast-scan cyclic voltammetry (FSCV) is an established method for measuring dopamine (DA) levels in the brain in real time. However, it is difficult to discriminate DA from other monoamines such as serotonin (5-hydroxytryptamine, 5-HT) and norepinephrine (NE). We report a novel DA-specific biosensor consisting of a car...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.24193

    authors: Ishida A,Imamura A,Ueda Y,Shimizu T,Marumoto R,Jung CG,Hida H

    更新日期:2018-05-01 00:00:00

  • Coexpression of Brn-3a POU protein with p53 in a population of neuronal progenitor cells is associated with differentiation and protection against apoptosis.

    abstract::The Brn-3a transcription factor is critical for survival and differentiation of sensory neurons derived from neural crest cells (NCC). Interaction of Brn-3a with p53 results in differential effects on target gene expression, which profoundly affects fate of neuronal cells. Here we demonstrate colocalization of p53 in ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.20299

    authors: Hudson CD,Podesta J,Henderson D,Latchman DS,Budhram-Mahadeo V

    更新日期:2004-12-15 00:00:00

  • Characterization of synaptic vesicles and related neuronal features in nerve growth factor and ras oncogene differentiated PC12 cells.

    abstract::PC12 cells can differentiate into neuron-like cells after treatment with either nerve growth factor (NGF) or transduction with a retrovirus which expresses the K-ras oncogene. The concomitant treatment of NGF plus ras differentiates PC12 cells further than either agent alone with respect to neurite outgrowth, acetylch...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490420306

    authors: Tao-Cheng JH,Dosemeci A,Bressler JP,Brightman MW,Simpson DL

    更新日期:1995-10-15 00:00:00

  • Effects of blinding and pinealectomy on regional brain monoamine concentrations.

    abstract::The effects of blinding with or without pineal ablation on brain monoamine levels were studied in male rats. Brain dopamine (DA), norepinephrine (NE), epinephrine (E), and serotonin (5-HT) were measured by radioenzymatic assays. Four weeks following pinealectomy, E levels were significantly enhanced in the frontal cor...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490100107

    authors: Niles LP,Brown GM,Mishra RK

    更新日期:1983-01-01 00:00:00

  • Preferential interneuron survival in the transition zone of 3-NP-induced striatal injury in rats.

    abstract::Histology, immunohistochemistry, and Western blotting were used to characterize the changes in morphology, distribution pattern, and marker protein expression of striatal interneurons in the transition zone of striatal injury induced by 3-NP. The 3-NP treatment in rats yielded movement, motor coordination, and cogniti...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.22591

    authors: Mu S,OuYang L,Liu B,Zhu Y,Li K,Zhan M,Liu Z,Jia Y,Lei W,Reiner A

    更新日期:2011-05-01 00:00:00

  • Release of polyunsaturated fatty acids from phospholipids and alteration of brain membrane integrity by oxygen-derived free radicals.

    abstract::We studied the effects of oxygen-derived free radicals on the ultrastructure of brain cortical slices and the release of fatty acids from phospholipids of crude synaptosomes. Xanthine oxidase, hypoxanthine, and ADP-Fe3+, a free-radical-generating system, caused swelling of cellular processes and mitochondria. The oxyg...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490120408

    authors: Chan PH,Fishman RA,Schmidley JW,Chen SF

    更新日期:1984-01-01 00:00:00

  • Hormones and growth factors induce the synthesis of glial fibrillary acidic protein in rat brain astrocytes.

    abstract::Glial fibrillary acidic protein (GFAP) is the major constituent of glial filaments and is restricted within the CNS to astrocytes. As with other classes of intermediate filament proteins, the regulation of GFAP expression is poorly understood. Utilizing highly purified cultures of astrocytes and a chemically defined (...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490140202

    authors: Morrison RS,De Vellis J,Lee YL,Bradshaw RA,Eng LF

    更新日期:1985-01-01 00:00:00

  • Novel antigenic determinant expressed in neurons of the dorsolateral hypothalamus in rat and human.

    abstract::Previous studies have identified a group of cells in the dorsolateral hypothalamus that project to many different areas in the CNS, such as thalamus, diagonal band of Broca, basal ganglia, cerebral cortex, hippocampus, and olfactory bulb. Their role is presently unknown, but the cells have been reported to stain for a...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490310415

    authors: Quinn J,Eckenstein FP,Baughman RW

    更新日期:1992-04-01 00:00:00

  • Detection of increased tissue concentrations of nerve growth factor with an improved extraction procedure.

    abstract::Nerve growth factor (NGF) is a protein essential for the survival and normal function of sympathetic neurons. Two-site immunoassays have been developed over the past decade in several laboratories and used to estimate its endogenous concentrations in a variety of effector tissues. However, levels appear restricted to ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4547(19961201)46:5<581::AID-JNR

    authors: Zettler C,Bridges DC,Zhou XF,Rush RA

    更新日期:1996-12-01 00:00:00

  • Regulation of membrane association and kinase activity of Cdk5-p35 by phosphorylation of p35.

    abstract::Although protein kinase Cdk5-p35 is important in many aspects of the development and function of the central nervous system, relatively little is known about its regulation. In the present study, we examined the relationship between the association of this kinase with membranes and its activity in perinatal and adult ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.21438

    authors: Sato K,Zhu YS,Saito T,Yotsumoto K,Asada A,Hasegawa M,Hisanaga S

    更新日期:2007-11-01 00:00:00

  • Timing the doxycycline yields different patterns of genomic recombination in brain neurons with a new inducible Cre transgene.

    abstract::We have developed a transgenic mouse expressing the Cre recombinase under control of a tetracycline-responsive promoter. Using a CamKIIalpha-driven tTA transgenic strain and a lacZ reporter mouse, we obtained the expected neuronal pattern of recombination in the olfactory lobe, cortex, striatum, hippocampus and Purkin...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.10213

    authors: Lindeberg J,Mattsson R,Ebendal T

    更新日期:2002-04-15 00:00:00

  • Isolation and partial amino acid sequence analysis of nerve growth factor from the guinea pig prostate.

    abstract::Nerve growth factor (NGF) has been purified from the guinea pig prostate using a modification of the Bocchini-Angeletti method for isolating 2.5S NGF from mouse submaxillary glands. As with the mouse preparation, guinea pig prostate NGF appears to migrate as a high molecular weight entity at physiological pH. Followin...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490060403

    authors: Rubin JS,Bradshaw RA

    更新日期:1981-01-01 00:00:00

  • Cortactin colocalizes with filopodial actin and accumulates at IgCAM adhesion sites in Aplysia growth cones.

    abstract::Both IgCAMs and the actin cytoskeleton play critical roles in neuronal growth cone motility and guidance. However, it is unclear how IgCAM receptors transduce signals from the plasma membrane to induce actin remodeling. Previous studies have shown that local clustering and immobilization of apCAM, the Aplysia homolog ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.21937

    authors: Decourt B,Munnamalai V,Lee AC,Sanchez L,Suter DM

    更新日期:2009-04-01 00:00:00

  • Differential expression of cytochrome oxidase (COX) genes in different regions of monkey brain.

    abstract::A frontal pole cDNA library from monkey (Macaca mulatta) brain was screened to identify mRNAs that are expressed more in frontal pole as compared to primary visual cortex. Three cDNA clones, whose greater expression was confirmed by Northern blot analysis, were identified as cytochrome oxidase (COX) subunits I, II, an...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490320313

    authors: Chandrasekaran K,Stoll J,Giordano T,Atack JR,Matocha MF,Brady DR,Rapoport SI

    更新日期:1992-07-01 00:00:00

  • Elevated serotonin is involved in hyperactivity but not in the paradoxical effect of amphetamine in mice neonatally lesioned with 6-hydroxydopamine.

    abstract::The neonatal lesion with 6-hydroxydopamine (6-OHDA) in rodents induces juvenile hyperactivity and paradoxical hypolocomotor response to psychostimulants, in striking contrast to what is observed when similar lesions are carried out in adults. The early disruption of central dopaminergic pathways is followed by increas...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.20245

    authors: Avale ME,Nemirovsky SI,Raisman-Vozari R,Rubinstein M

    更新日期:2004-10-15 00:00:00

  • Septohippocampal adaptive GABAergic responses by AF64A treatment.

    abstract::A cholinergically disrupted laboratory animal has been produced by administration of the cholinotoxin ethylcholine aziridinium mustard (AF64A), which produced a dysfunction in the cholinergic forebrain system. After AF64A treatment, a reduction of choline acetyl transferase (ChAT) activity was measured in the hippocam...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4547(19990115)55:2<178::AID-JNR

    authors: Ayala-Grosso CA,Urbina-Paez R

    更新日期:1999-01-15 00:00:00

  • Noninvasive early detection of brain edema in mice by near-infrared light scattering.

    abstract::Brain edema accounts for significant morbidity and mortality in many neurologic conditions such as head trauma, stroke, meningitis, and brain tumor. The water channel aquaporin-4 (AQP4) has been found to be an important determinant of brain water accumulation and clearance of excess brain water. We report the developm...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.20439

    authors: Thiagarajah JR,Papadopoulos MC,Verkman AS

    更新日期:2005-04-15 00:00:00

  • Hyposmolarity-induced taurine release in cerebellar granule cells is associated with diffusion and not with high-affinity transport.

    abstract::The effects of hyposmotic conditions on taurine uptake and release were studied in mice cultured cerebellar granule cells. The effect of DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulfonate) and of the divalent cations Mg++ and Mn++ on the hyposmolarity-induced changes in these parameters was investigated. Mg++ (20 mM...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490300409

    authors: Schousboe A,Sánchez Olea R,Morán J,Pasantes-Morales H

    更新日期:1991-12-01 00:00:00

  • Pharmacological characterisation of voltage-sensitive calcium channels and neurotransmitter release from mouse cerebellar granule cells in culture.

    abstract::Using subtype-specific Ca-channel blockers, we have characterised the voltage-sensitive Ca2+ currents as well as neurotransmitter release from cultured mouse cerebellar granule cells. The whole cell version of the patch clamp technique was adapted to monitor the isolated Ca-channel currents. The currents were activate...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-4547(19970401)48:1<43::aid-jnr4

    authors: Varming T,Christopherson P,Schousboe A,Drejer J

    更新日期:1997-04-01 00:00:00

  • Suppression of CXCL2 upregulation underlies the therapeutic effect of the retinoid Am80 on intracerebral hemorrhage in mice.

    abstract::We previously demonstrated that a synthetic retinoic acid receptor agonist, Am80, attenuated intracerebral hemorrhage (ICH)-induced neuropathological changes and neurological dysfunction. Because inflammatory events are among the prominent features of ICH pathology that are affected by Am80, this study investigated th...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.23379

    authors: Matsushita H,Hijioka M,Ishibashi H,Anan J,Kurauchi Y,Hisatsune A,Seki T,Shudo K,Katsuki H

    更新日期:2014-08-01 00:00:00

  • Structure and function of long-lived olfactory organotypic cultures from postnatal mice.

    abstract::The first synapse in the olfactory pathway mediates a significant transfer of information given the restricted association of specific olfactory receptor neurons with specific glomeruli in the olfactory bulb. To understand better how this connection is made and what the functional capacities of the participating cells...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.20007

    authors: Josephson EM,Yilma S,Vodyanoy V,Morrison EE

    更新日期:2004-03-01 00:00:00

  • Phosphofructokinase in the rat nervous system: regional differences in activity and characteristics of axonal transport.

    abstract::The distribution of phosphofructokinase (PFK) in gray and white matter regions of the rat nervous system was evaluated. Determinations of PFK activity revealed that cell body enriched regions (sensorimotor cortex) had a significantly higher level of activity than axonal regions (sciatic nerve, dorsal roots, and optic ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490210105

    authors: Oblinger MM,Foe LG,Kwiatkowska D,Kemp RG

    更新日期:1988-09-01 00:00:00

  • Regulation of thyroid hormone receptor and c-erbA mRNA levels by butyrate in neuroblastoma (N2A) and glioma (C6) cells.

    abstract::Butyrate produced a biphasic modulation of the thyroid hormone receptor in neuroblastoma N2A cells increasing receptor number by 20-35% at concentrations 0.25-0.75 mM and decreasing receptor levels by 30-55% at 2-4 mM. The half-life of the receptor, as assessed by its disappearance after incubation with 18 microM cycl...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490270102

    authors: Yusta B,Ortiz-Caro J,Bedo G,Pascual A,Aranda A

    更新日期:1990-09-01 00:00:00

  • Endogenous erythropoietin from astrocyte protects the oligodendrocyte precursor cell against hypoxic and reoxygenation injury.

    abstract::The hypoxia-responsive cytokine erythropoietin (EPO) provides neuroprotective effects in the damaged brain during ischemic events and neurodegenerative diseases. The purpose of the present study is to evaluate the EPO/EPO receptor (EPOR) endogenous system between astrocyte and oligodendrocyte precursor cell (OPC) unde...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.22702

    authors: Kato S,Aoyama M,Kakita H,Hida H,Kato I,Ito T,Goto T,Hussein MH,Sawamoto K,Togari H,Asai K

    更新日期:2011-10-01 00:00:00

  • Effect of rosmarinic acid in motor dysfunction and life span in a mouse model of familial amyotrophic lateral sclerosis.

    abstract::Amyotrophic lateral sclerosis (ALS) is a late-onset progressive neurodegenerative disease affecting motor neurons. About 2% of patients with the disease are associated with mutations in the gene encoding Cu/Zn superoxide dismutase (SOD1). The purpose of this study is to assess the effect of rosemary extract and its ma...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.22242

    authors: Shimojo Y,Kosaka K,Noda Y,Shimizu T,Shirasawa T

    更新日期:2010-03-01 00:00:00

  • Controlled contusion injury alters molecular systems associated with cognitive performance.

    abstract::We investigated whether a learning impairment after a controlled cortical impact (CCI) injury was associated with alterations in molecules involved in synaptic plasticity and learning and memory. Adult male rats with moderate CCI to the left parietal cortex, tested in a Morris water maze (MWM) beginning at postinjury ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.21893

    authors: Griesbach GS,Sutton RL,Hovda DA,Ying Z,Gomez-Pinilla F

    更新日期:2009-02-15 00:00:00

  • Neuroinflammation and regulation of glial glutamate uptake in neurological disorders.

    abstract::Oxidative stress, neuroinflammation, and excitotoxicity are frequently considered distinct but common hallmarks of several neurological disorders, including Parkinson's disease, amyotrophic lateral sclerosis, multiple sclerosis, and Alzheimer's disease. Although neuron degeneration and death are the ultimate consequen...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审

    doi:10.1002/jnr.21325

    authors: Tilleux S,Hermans E

    更新日期:2007-08-01 00:00:00

  • Experimental acute traumatic injury of the adult rat spinal cord by a subdural inflatable balloon: methodology, behavioral analysis, and histopathology.

    abstract::We describe an experimental model to produce closed traumatic injuries to the spinal cord of adult rats. This model uses an inflatable balloon that is introduced in the dorsal subdural space and moved to a location rostral to the laminectomy site. The spinal cord trauma can be graded by varying either the duration of ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490320409

    authors: Martin D,Schoenen J,Delrée P,Gilson V,Rogister B,Leprince P,Stevenaert A,Moonen G

    更新日期:1992-08-01 00:00:00