Abstract：
:This study examined the growth of basal plate explants from 40-hr chick embryos containing the trigeminal motor nucleus in the presence of target muscle-conditioned medium (MCM) from day-4, day-10, and day-18 embryos. We had previously found that MCM derived from the 4-day target tissue enhanced neurite outgrowth from these explants, while target MCM from 10- and 18-day embryos inhibited it. For the present study, target MCM was treated with trypsin to assess the nature of the active fractions, or pre-incubated in polyornithine-coated dishes to determine the possible presence and relative contributions of substrate-binding vs. soluble components. Both trypsinization and pre-incubation abolished the outgrowth-enhancing potential of the 4-day MCM, indicating a protein or polypeptide substrate-binding active fraction, with no appreciable active soluble component. When the 10-day and 18-day target MCM were trypsinized, the inhibitory influence was reversed, and neurite outgrowth was enhanced. Similarly, when the MCM from these embryos was pre-incubated over polyornithine, the inhibitory influence was lost and was replaced by a stimulatory one. These results suggest that the 10- and 18-day MCM contain an active inhibitory fraction that is a protein or polypeptide, and which binds to a positively charged substrate. When this inhibitory fraction is inactivated or depleted, an excitatory soluble fraction is revealed. Such a dual nature in MCM has not been seen previously and may be expressed only when the media are assayed with very early neural tissue.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Heaton MBdoi
10.1002/jnr.490190109subject
Has Abstractpub_date
1988-01-01 00:00:00pages
57-61issue
1eissn
0360-4012issn
1097-4547journal_volume
19pub_type
杂志文章abstract：:Amyotrophic lateral sclerosis (ALS) is a late-onset progressive neurodegenerative disease affecting motor neurons. About 2% of patients with the disease are associated with mutations in the gene encoding Cu/Zn superoxide dismutase (SOD1). The purpose of this study is to assess the effect of rosemary extract and its ma...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22242
更新日期：2010-03-01 00:00:00
abstract：:Six different types of spectral responses were recorded from horizontal cells under mesopic conditions in perfused retina, isolated from the dark-adapted mojarra (Eugerres plumieri). They were tentatively termed photopic Lr-, Lg1-, Lg2-, Lb-, and C-type, and scotopic L-type. The Lr-, Lg-, and Lb-type responses showed ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490200214
更新日期：1988-01-01 00:00:00
abstract：:The concept of the neurovascular unit emphasizes that common signals and substrates underlie the physiology and pathophysiology of neuronal and endothelial compartments in brain. Recent data suggest that activation of the integrin-associated protein CD47 promotes neuronal cell death. Is it possible that CD47 may also ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22076
更新日期：2009-08-15 00:00:00
abstract：:The galactolipids galactocerebroside and sulfatide have been implicated in oligodendrocyte (OL) development and myelin formation. Much of the early evidence for myelin galactolipid function has been derived from antibody and chemical perturbation of OLs in vitro. To determine the role of these lipids in vivo, we previ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22003
更新日期：2009-11-15 00:00:00
abstract：:Cultured rat mesencephalic neurons were used to assess the effects of gamma-aminobutyric acid (GABA) transport blockers on toxicity caused by malonate, a reversible, competitive inhibitor of succinate dehydrogenase. Previous studies utilizing an ex vivo chick retinal preparation have shown that GABA release and cell s...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.1052
更新日期：2001-04-01 00:00:00
abstract：:The incidence of dementia and obstructive sleep apnea (OSA) increases with age. Late-onset Alzheimer's disease (AD) is an irreversible neurodegenerative disease of the elderly characterized by amyloid β (Aβ) plaques and neurofibrillary tangles. The disease involves widespread synaptic loss in the neocortex and the hip...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.23634
更新日期：2015-12-01 00:00:00
abstract：:Peroxides are generated continuously in cells that consume oxygen. Among the different peroxides, hydrogen peroxide is the molecule that is formed in highest quantities. In addition, organic hydroperoxides are synthesized as products of cellular metabolism. Generation and disposal of peroxides is a very important proc...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.20280
更新日期：2005-01-01 00:00:00
abstract：:Among the ion channels and pumps activated by growth factor stimulation, K+ channels have been implicated in the growth and proliferation of several cancer cell lines. The role of these channels in central nervous system tumors, however, has not been described. This study used the malignant astrocytoma cell lines U87 ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：
更新日期：1997-04-15 00:00:00
abstract：:Post-translational modification of neural cell adhesion molecule (NCAM) with alpha2,8-linked polysialic acid, which regulates homophilic adhesion and/or signal transduction events, is crucial to synaptic plasticity in the developing and adult brain. Evidence from in vitro models has implicated polysialylation in the r...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/1097-4547(20000915)61:6<636::AID-JNR7>3.0.
更新日期：2000-09-15 00:00:00
abstract：:The cholinergic neurons of the basal forebrain (CNBF) are the major source of cholinergic innervation of the cortex and hippocampus. In Alzheimer's disease and aged brain, there are severe losses of cholinergic neurons in the nucleus basalis of Meynert, leading to a reduction of cortical cholinergic activity which cor...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19980501)52:3<322::AID-JNR
更新日期：1998-05-01 00:00:00
abstract：:Mg2+ATPase and (Na+ + K+)ATPase activities were measured in clonal line NN hamster astroblasts and in clonal lines M1 and N1E-115 mouse neuroblastoma cells after the cells had been subjected to the acute and chronic actions of 100 mM ethanol. Exposure of the astroblasts to ethanol for periods as long as 68 days produc...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490020205
更新日期：1976-01-01 00:00:00
abstract：:Cultured Schwann cells treated with heregulin growth factor require costimulation with a cyclic adenosine monophosphate-elevating agent to produce maximal cell proliferation. Gene chip expression analysis was used to identify genes that are induced or repressed when Schwann cells are treated with heregulin and/or fors...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10679
更新日期：2003-08-15 00:00:00
abstract：:The inner ear of humans and experimental animals demonstrate an abundance of glucocorticoid receptors (GR). Glucocorticoids (GC) are widely used to treat different hearing disorders; yet the mechanisms of GC action on the inner ear are unknown. We demonstrate how GR can directly modulate hearing sensitivity in respons...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20795
更新日期：2006-05-01 00:00:00
abstract：:The higher prevalence and risk for Alzheimer's disease in women relative to men has been partially attributed to the precipitous decline in gonadal hormone levels that occurs in women following the menopause. Although considerable attention has been focused on the consequence of estrogen loss, and thus estrogen's neur...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21370
更新日期：2007-08-15 00:00:00
abstract：:Lithium, the most prevalent treatment for manic-depressive illness, might have a neuroprotective effect after brain injury. In culture, lithium can exert neurotoxic effects associated with reduction in polyamine synthesis but neuroprotective effects as cultured neurons mature. Cumulative evidence suggests that lithium...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21152
更新日期：2007-02-15 00:00:00
abstract：:Increase of intracellular [Ca(2+)] evoked by action potentials in a cell can induce long-term synaptic plasticity even without concomitant presynaptic stimulation. We used optical recording of the fluorescence of a Ca(2+)-indicator Oregon Green to investigate whether differences in results obtained with modifications ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20104
更新日期：2004-05-15 00:00:00
abstract：:Identification of behavioral loci through complex trait mapping remains a widely employed approach but suffers from poor gene localization and low replicability. Genome-tagged mice (GTMs) are overlapping sets of congenic strains spanning the whole genome and offer the possibilities of superior mapping power and reprod...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10765
更新日期：2003-11-15 00:00:00
abstract：:The role of the NT-3 has been implicated in the survival of progenitor oligodendrocytes in culture. The object of this study was to investigate the expression of the TrkC receptor and its responsiveness in glial cells. We report the expression of two TrkC receptor isoforms in rat primary oligodendrocyte cultures, a gl...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19960601)44:5<490::AID-JNR
更新日期：1996-06-01 00:00:00
abstract：:Mutations in the beta-amyloid precursor protein (APP) gene cause familial Alzheimer's disease (AD). Although amyloid beta peptide (Abeta) is the principal constituent of senile plaques in AD, other cleavage products of APP are also implicated in playing a role in the pathogenesis of AD. C-terminal fragments of APP (AP...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(20000515)60:4<565::AID-JNR
更新日期：2000-05-15 00:00:00
abstract：:Thymocyte differentiation antigen-1 (Thy-1) is a cell surface glycoprotein found on T cells and neurons and is involved in cell-to-cell interactions. In addition, Thy-1 knockouts (KO) are a potential mouse model of restless legs syndrome (RLS) based on clinical observations and the role of dopamine in the disease. In ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23046
更新日期：2012-08-01 00:00:00
abstract：:Insulin-like growth factor II (IGF-II) plays significant roles in the growth and development of mammals through the regulation of mitogenesis and cell survival. Previously, IGF-II mRNA transcripts within the CNS were detected in the choroid plexus and leptomeninges (DeChiara et al., 1991). The objective of this study ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19971215)50:6<958::AID-JNR
更新日期：1997-12-15 00:00:00
abstract：:The contribution of glucose carbon to the biosynthesis of cholesterol and phospholipids in distinct brain regions was studied quantitatively in the adult male rat. Rates of flow of glucose carbon into the lipids in vivo were calculated from two measurements: the curve representing the decrease in plasma 14C-glucose wi...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490060505
更新日期：1981-01-01 00:00:00
abstract：:Retinal microglia play an important role as resident immunocompetent and phagocytic cells in the event of injury and disease. Retinal microglia and microglia precursor transplantation show a rescue effect in ischemic retina and retinal degeneration. However, studies of retinal microglia have been hampered by the diffi...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23337
更新日期：2014-04-01 00:00:00
abstract：:The expression of GABAB receptors in cultured mouse cerebellar granule cells was investigated in binding experiments using [3H](S,R)-baclofen as well as in functional assessment of the ability of (R)-baclofen to interact with depolarization (15-40 mM KCl) coupled changes in intracellular Ca2+ homeostasis and neurotran...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490390604
更新日期：1994-12-15 00:00:00
abstract：:Calcium/calmodulin-dependent serine kinase (CASK), a causative gene in X-linked mental retardation, carries out multiple functions in neurons, including vesicle trafficking of ion channels, synapse formation, and gene transcription. From a yeast two-hybrid screen, Krüppel-like zinc finger protein B cell lymphoma/COUP-...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22407
更新日期：2010-08-15 00:00:00
abstract：:Cultures of dissociated hypothalamic cells taken from rat fetuses of 19 days of gestation were studied using time-lapse recording and sequential microphotography from 1 to 5 days in vitro (DIV) and at 7 and 21 DIV. Cultures were seeded with cells taken from fetuses grouped by sex or sexually mixed; experimental cultur...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490330210
更新日期：1992-10-01 00:00:00
abstract：:We studied the effects of electrical stimulation of the inferior alveolar nerve (IAN) on cardiovascular responses in cats. There was statistical correlation between cardiovascular response and prestimulus mean arterial blood pressure (MABP) and heart rate (HR). A trigeminal depressor response (TDR) was induced when th...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20131
更新日期：2004-06-15 00:00:00
abstract：:CD200, a type I transmembrane glycoprotein, can interact with its receptor CD200R, which plays an inhibitory role in the activation of microglia-the resident macrophages of the central nervous system. In this study, the rat C6 glioma cell line (C6-1) that was previously characterized with high in vivo tumorigenicity w...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23922
更新日期：2016-12-01 00:00:00
abstract：:Mitochondria are power organelles generating biochemical energy, ATP, in the cell. Mitochondria play a variety of roles, including integrating extracellular signals and executing critical intracellular events, such as neuronal cell survival and death. Increasing evidence suggests that a cross-talk mechanism between mi...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.21564
更新日期：2008-04-01 00:00:00
abstract：:Intrinsic mechanisms that guide damaged axons to regenerate following spinal cord injury remain poorly understood. Manipulation of posttranslational modifications of key proteins in mature neurons could reinvigorate growth machinery after injury. One such modification is acetylation, a reversible process controlled by...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23573
更新日期：2015-08-01 00:00:00
