Abstract：
:Calcium/calmodulin-dependent serine kinase (CASK), a causative gene in X-linked mental retardation, carries out multiple functions in neurons, including vesicle trafficking of ion channels, synapse formation, and gene transcription. From a yeast two-hybrid screen, Krüppel-like zinc finger protein B cell lymphoma/COUP-TF-interacting protein 1 (Bcl11A/CTIP1) was identified as a CASK binding protein. Through alternative splicing, a single Bcl11A gene encodes two major protein products in neurons, Bcl11A-S and Bcl11A-L. CASK interacted with both Bcl11A-S and Bcl11A-L in transfected COS cells and brain. Immunofluorescence staining further indicated the colocalization of CASK and Bcl11A in the nuclei of neurons. These studies supported an interaction between CASK and Bcl11A in vivo. Bcl11A-L has previously been shown to play a role in gene transcription as well as axon outgrowth and branching. Here, we further show that Bcl11A-L rearranges the distribution of nuclear actin, which may be related to the function of Bcl11A-L in gene expression. More importantly, using cultured hippocampal neurons as a model system, we show that CASK enhances the ability of Bcl11A-L to restrict axon outgrowth and branching. Interruption of the interaction between CASK and Bcl11A increased the outgrowth and branching of axons, suggesting that the interaction between CASK and Bcl11A controls axon arborization. In conclusion, our results suggest that, through the interaction with Bcl11A, CASK plays a role in axonogenesis, which may be related to brain anatomical characteristics in humans.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Kuo TY,Hong CJ,Chien HL,Hsueh YPdoi
10.1002/jnr.22407subject
Has Abstractpub_date
2010-08-15 00:00:00pages
2364-73issue
11eissn
0360-4012issn
1097-4547journal_volume
88pub_type
杂志文章abstract：:Recent data indicate that molecular mimicry may play a role in the pathogenesis of human-T-lymphotropic virus type-1 (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP), an immune-mediated disease of the central nervous system (CNS). Specifically, HAM/TSP patients developed antibodies that cross-reac...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20137
更新日期：2004-07-01 00:00:00
abstract：:Monoclonal antibody J1-31 (MAb J1-31, isotype IgG 2b) was raised against crude homogenate of brain tissue from a multiple sclerosis (MS) patient (autopsy sample; Malhotra et al.: Microbios Letters 26:151-157, 1984). In human brain, MAb J1-31 recognizes an intracellular protein antigen (J1-31 antigen), which bands at a...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490220106
更新日期：1989-01-01 00:00:00
abstract：:Music has a long history of healing or mitigating physical and mental illness in the clinical setting. We aimed to test changes in behavioral cognition and serum proteomics in rats undergoing music intervention (MI). The Morris water maze (MWM) was used to evaluate spatial learning and memory in rats. Serum protein ex...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.24275
更新日期：2018-10-01 00:00:00
abstract：:The effect of two different potassium channel blockers, 4-aminopyridine (4-AP) and quinine, on the alpha 2-adrenoceptor mediated modulation of norepinephrine (NE) release was investigated. Pairs of mouse vasa deferentia were loaded with 3H-norepinephrine (3H-NE), superfused continuously, and stimulated electrically. 4...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490200114
更新日期：1988-05-01 00:00:00
abstract：:We have developed a silicone nerve regeneration chamber that is partitioned into two compartments by a strip of nitrocellulose paper. The modified two-compartment chamber allows the investigation of the effects on rat sciatic nerve regeneration of trophic or growth factors that are initially bound to the nitrocellulos...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490200306
更新日期：1988-07-01 00:00:00
abstract：:In this study we used as glial cell models, early and late passage C-6 glial cells, 2B clone, and advanced passages of glial cells derived from aged mouse cerebral hemispheres (MACH) to examine responsiveness to opioids. We have previously reported that early passage C-6 glial cells, 2B clone, are bipotential and can ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490360509
更新日期：1993-12-01 00:00:00
abstract：:Previously, we reported the presence of multiple neurotransmitters in subclones of SN6, a septal cholinergic hybrid cell line. To obtain information concerning the functionality of these transmitters, we measured transmitter contents, activities of transmitter-producing enzymes, and the effect of serum-free culture me...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490420607
更新日期：1995-12-15 00:00:00
abstract：:The two inbred strains of mice C57BL/6 (alcohol-preferring) and DBA/2 (alcohol-avoiding) mice have been shown to differ significantly in their preference for alcohol (EtOH). We have previously demonstrated the differences in the density and the affinity of cannabinoid (CB1) receptors in the brains of the two inbred C5...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.1094
更新日期：2001-05-15 00:00:00
abstract：:The role of insulin-like growth factor-1 (IGF-1) in amyotrophic lateral sclerosis (ALS) and its mechanism of action are important from both pathogenic and therapeutic points of view. The present study investigated the changes of IGF-1Rbeta and the key intracellular downstream protein insulin receptor substrate-1 (IRS-...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20668
更新日期：2005-11-15 00:00:00
abstract：:Our aim has been to investigate the ability of the rat brain to retain its level of neurotransmitter release over life. We have investigated the neurotransmitter release from the rat brain synaptosomes prelabeled with 3H-DA, 3H-NA, or 3H-5HT, and perfused with Krebs-Ringer medium alone (basal release) or containing a ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490340314
更新日期：1993-02-15 00:00:00
abstract：:Sphingolipidoses are a class of inherited diseases that result from the toxic accumulation of undigested sphingolipids in lysosomes and other cellular membranes. Sphingolipids are particularly enriched in cells of the nervous system, and their excessive accumulation during disease has a significant impact on the nervo...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.23763
更新日期：2016-11-01 00:00:00
abstract：:The distribution of both tyrosine hydroxylase (TH) antigen and messenger RNA was investigated in the ventral mesencephalon from human brain using a 35S-labeled human TH cDNA probe and a polyclonal TH antibody. A hybridization signal was detected not only in the perikaryon but also in the neurites, most probably dendri...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490250415
更新日期：1990-04-01 00:00:00
abstract：:Myelin gene expression in normal oligodendrocytes (OLG) depends on developmentally regulated protein kinase C (PKC) enzyme activity (Asotra and Macklin: J Neurosci Res 34:571-588, 1993). We studied the developmental expression of the Ca(++)-dependent PKC-alpha, -beta 1, -beta II and -gamma isozymes, and the Ca(++)-ind...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490390305
更新日期：1994-10-15 00:00:00
abstract：:This essay discusses the directional movements of metazoan tissue cells generally, with special emphasis on neurons, in an attempt to show that the directional movements of all share fundamental similarities. ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.490130102
更新日期：1985-01-01 00:00:00
abstract：:The periglomerular cells (PG) of the olfactory bulb (OB) are involved in the primary processing and the refinement of sensory information from the olfactory epithelium. The neurochemical composition of these neurons has been studied in depth in many species, and over the last decades such studies have focused mainly o...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22521
更新日期：2011-01-01 00:00:00
abstract：:Cognitive decline in Parkinson's disease (PD) is a common sequela of the disorder that has a large impact on patient well-being. Its physiological etiology, however, remains elusive. Our study used graph theory analysis to investigate the large-scale topological patterns of the extrastriatal dopamine D2 receptor netwo...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.24760
更新日期：2020-12-03 00:00:00
abstract：:The growth and differentiation of neural precursor cells in the central nervous system (CNS) are regulated by their response to polypeptide growth factors which interact with specific transmembrane receptor tyrosine kinases (RTKs). We demonstrate that rat oligodendrocyte-type 2 astrocyte (O-2A) glial progenitor cells,...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490360512
更新日期：1993-12-01 00:00:00
abstract：:Acetylcholinesterase (AChE) molecular forms were studied in normal (NM) and in dystrophic (DM) 129B6F1/J mouse muscle. Successive extractions of the tissue with saline and saline-Triton X-100 buffers yielded two soluble fractions, S1 and S2. Forty percent of the AChE in NM was measured in S1 and 60% in S2, and 65% and...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490380504
更新日期：1994-08-01 00:00:00
abstract：:Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that form a subfamily within the steroid hormone receptor group. Recent work has shown that one member of this group, PPARgamma, plays a central role in adipocyte differentiation. As oligodendrocytes are major lipid-producin...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19980301)51:5<563::AID-JNR
更新日期：1998-03-01 00:00:00
abstract：:The balance of bone morphogenic protein (BMP), transforming growth factor-β (TGFβ)/activin/nodal, and Wnt signals regulates the early lineage segregation of human embryonic stem cells (ESCs). Here we demonstrate that a combination of small-molecule inhibitors of BMP (Dorsomorphin) and TGFβ/activin/nodal (SB431542) sig...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22547
更新日期：2011-02-01 00:00:00
abstract：:Barbiturates are regularly used as an anesthetic for animal experimentation and clinical procedures and are frequently provided with solubilizing compounds, such as ethanol and propylene glycol, which have been reported to affect brain function and, in the case of (1)H NMR experiments, originate undesired resonances i...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22212
更新日期：2010-02-01 00:00:00
abstract：:Pelizaeus-Merzbacher disease (PMD) is an X-linked disorder of the central nervous system (CNS) caused by a wide variety of mutations affecting proteolipid protein 1 (PLP1). We assessed the effects of PLP1 mutations on water diffusion in CNS white matter by using diffusion tensor imaging. Twelve patients with different...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23458
更新日期：2014-12-01 00:00:00
abstract：:Epidural hematoma (EDH) is a type of life-threatening traumatic brain injury. Little is known about the extent to which EDH may cause neural damage and regenerative response in the cerebral cortex. Here we attempted to explore these issues by using guinea pigs as an experimental model. Unilateral EDH was induced by in...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23148
更新日期：2013-02-01 00:00:00
abstract：:Vascular smooth muscle (VSM) cells comprise one of the primary targets of the sympathetic nervous system and have been shown to secrete nerve growth factor (NGF). There is increasing evidence that changes in the levels of NGF in the adult may underlie certain pathological conditions. To investigate the potential role ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：
更新日期：1997-02-01 00:00:00
abstract：:Antibody-producing hybridoma cells specifically bind to microgram quantities of antigen molecules adsorbed onto the surface of plastic microtiter plates. The binding of hybridoma cells to nonantigen is optimally below 5%, similar binding of non-antibody-producing cells is 4-7%, compared to the binding of the hybridoma...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490150312
更新日期：1986-01-01 00:00:00
abstract：:The role of intracellular proteases (e.g., calpains and caspases) in the pathophysiology of neuronal cell death has been extensively investigated. More recently, accumulating data have suggested that extracellular proteolysis also plays a critical role. The two major systems that modify the extracellular matrix in bra...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.10270
更新日期：2002-07-01 00:00:00
abstract：:Cranial idiopathic acute peripheral facial nerve paralysis (Bell palsy) has been used as a model disease in the present study mainly to investigate trophic capacity of serum components. The ability to induce sprouting from chick embryo dorsal root ganglia was used as the bioassay for trophic activity. Sera from normal...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490090411
更新日期：1983-01-01 00:00:00
abstract：:Two fundamental questions about neuron cell culture were addressed. Can one serum-free medium that was developed for optimum growth of hippocampal neurons support the growth of neurons from other regions of the brain? Is the region specific state of differentiation maintained in culture? To answer these questions, we ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490420510
更新日期：1995-12-01 00:00:00
abstract：:Four brain-specific, DNase I hypersensitive sites (HSS) have been mapped to the 5' flanking region of the mouse 68-kDa neurofilament gene. These sites are contained within a 1.7-kb sequence that confers neuronal specificity of expression of this gene in transgenic mice. To identify DNA sequences that might be involved...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490320204
更新日期：1992-06-01 00:00:00
abstract：:Our previous work indicated that in E14 embryonic rat spinal cord cultures ciliary neuronotrophic factor (CNTF) exerted (1) a survival-promoting effect on motor neurons and on a large population of unidentified neurons, and (2) a regulatory role on the expression of ChAT and low affinity NGF receptor (LNGFR) in a popu...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490300314
更新日期：1991-11-01 00:00:00
