Abstract:
:Myelin gene expression in normal oligodendrocytes (OLG) depends on developmentally regulated protein kinase C (PKC) enzyme activity (Asotra and Macklin: J Neurosci Res 34:571-588, 1993). We studied the developmental expression of the Ca(++)-dependent PKC-alpha, -beta 1, -beta II and -gamma isozymes, and the Ca(++)-independent PKC-delta, -epsilon, -zeta and -eta isozymes in enriched rat brain OLG cultures. In A2B5+ O-2A progenitors, only PKC-delta, PKC-epsilon and PKC-zeta were detected immunocytochemically. In 04+ proligondendrocytes, PKC-beta I, -delta and -zeta were expressed moderately and low levels of PKC-alpha and -epsilon were detected. GD3+ OLG, GC+ OLG and MBP+ OLG showed increased levels of PKC-alpha, -beta I, -delta and -zeta isozymes. PKC-beta II, -gamma and -eta were poorly expressed in OLG. On immunoblots, PKC-alpha was present early and increased continually up to 18 days but PKC-beta I increased until 12 days in cultured OLG. High levels of PKC-delta, PKC-epsilon and PKC-zeta, the most abundant PKC isozymes in OLG, were maintained up to 12 days and were then slightly reduced. Interestingly, relatively high levels of PKC-alpha, PKC-beta I, PKC-beta II, PKC-gamma and PKC-epsilon isozymes were detected in purified myelin membrane although greater levels of PKC-delta were found in OLG than in purified myelin. Thus, most of the PKC isozymes found in cultured OLG were also present in myelin, although at different levels. Treatment with 50 nM 4 beta-phorbol-12,13-dibutyrate (PDB) caused a delayed downregulation of PKC-delta levels after 8 hr without modulating the expression of other PKC isozymes in 1-day OLG; in the 3-day-old and 6-day-old OLG, PDB downmodulated PKC-beta I, -delta and epsilon isozymes with only a minor effect on PKC-alpha and no reduction in PKC-zeta. Induction or downmodulation of individual PKC isozymes by phorbol esters appears to depend on the differentiation state of OLG. These data suggest that PKC-beta I, -delta and -epsilon isozymes have an important function in different cellular events of OLG differentiation. We conclude that the PKC-dependent modulation of myelin gene expression in OLG results predominantly from the Ca(++)-dependent PKC-beta I isozyme activity and the CA(++)-independent PKC-delta and PKC-epsilon activitives in a cell differentiation state-dependent manner.(ABSTRACT TRUNCATED AT 400 WORDS)
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Asotra K,Macklin WBdoi
10.1002/jnr.490390305subject
Has Abstractpub_date
1994-10-15 00:00:00pages
273-89issue
3eissn
0360-4012issn
1097-4547journal_volume
39pub_type
杂志文章abstract::Pinealocytes of various mammalian species contain abundant synaptic-like microvesicles (SLMVs) which are considered the endocrine equivalent of neuronal synaptic vesicles. Although the pinealocytes may thus be a suitable cellular model for experimental in vitro studies of SLMVs, nothing is known about the presence of ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(sici)1097-4547(19970301)47:5<509::aid-jnr
更新日期:1997-03-01 00:00:00
abstract::Glucocorticoid hormones affect gene expression directly at the level of transcription via intracellular receptors that translocate to the nucleus in the presence of steroid. In the brain, two types of high-affinity receptors bind glucocorticoids, the type I, mineralocorticoid receptor and the type II, glucocorticoid r...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490270315
更新日期:1990-11-01 00:00:00
abstract::The growth cone, the motile tip of developing neuronal processes, is considered responsible for the exact guidance of axons and synaptogenesis. High activity of tyrosine kinases in growth cones may contribute to the functions of growth cones. Our previous work revealed that vinculin is one of the endogenous substrates...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490300127
更新日期:1991-09-01 00:00:00
abstract::We have used adult mouse superior cervical ganglia (SCG) to study the role of mitogen activated protein (MAP) kinase activity during axonal outgrowth in vitro. An initial peak in activity within the first hours of culture was followed by a substantially higher activity after 1 to 2 days, a time when axons were activel...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19980515)52:4<453::AID-JNR
更新日期:1998-05-15 00:00:00
abstract::gamma-Aminobutyric acid (GABA) regulates the proliferation and migration of olfactory bulb (OB) interneuron progenitors derived from the subventricular zone (SVZ), but the role of GABA in the differentiation of these progenitors has been largely unexplored. This study examines the role of GABA in the differentiation o...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22055
更新日期:2009-08-01 00:00:00
abstract::The cellular constituents of the blood-brain barrier (BBB) must make finely tuned, regulatory responses to maintain microvascular homeostasis. The mechanisms by which this task is accomplished are largely unknown. However, it is thought they involve a series of cross-talk mechanisms among endothelial cells (EC), peric...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19990301)55:5<578::AID-JNR
更新日期:1999-03-01 00:00:00
abstract::Larval acetylcholinesterase (acetylcholine acetylhydrolase) EC 3.1.3.7 of the trematode Schistosoma mansoni was characterized and purified by affinity chromatography. The enzyme was solubilized from sonicated cercarial tissue and showed a Km value of 1.83 mM and a Vmax value of 102 U/mg protein. It was characterized a...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490150413
更新日期:1986-01-01 00:00:00
abstract::To promote neurite elongation, nerve growth cones must adhere to other surfaces. A complex of integral membrane glycoproteins mediates cell binding to the extracellular glycoproteins fibronectin and laminin (Horwitz et al., J Cell Biol 101:2134-2144, 1985). The receptor complex, named integrin, binds to fibronectin by...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490210221
更新日期:1988-10-01 00:00:00
abstract::Using [3H]-dopamine (DA) as the ligand of choice, we have shown that rat lymphocytes possess DA binding sites. The binding of [3H]-DA was specific, saturable, and of high affinity. The specific binding in membranes obtained from Con A-activated lymphoblasts was greater than the binding in respective unactivated lympho...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490180112
更新日期:1987-01-01 00:00:00
abstract::In this study, we examined the neuroprotective effect of standardized Bacopa monniera extract (BME: BESEB CDRI-08) against the D-galactose (D-gal)-induced brain aging in rats. Experimental groups were subjected to contextual-associative learning task. We found that the administration of BME in the D-gal-treated group ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23080
更新日期:2012-10-01 00:00:00
abstract::In recent years, several novel types of disorders have been characterized, including what have been termed polyalanine diseases, in which patients have expanded triplet repeats in specific genes, resulting in the translation of aberrantly elongated polyalanine stretches. In this study, we showed that yellow fluorescen...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21619
更新日期:2008-05-15 00:00:00
abstract::We have studied the effect of induced glial fibrillary acidic protein (GFAP) on motility, cell morphology, and proliferation of two originally GFAP-negative human glioma cell lines. Glioma cell lines U-1242 MG and U-251 MG sp subclone 3A were transfected with a vector system that allows for an inducible GFAP expressio...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(20000415)60:2<245::AID-JNR
更新日期:2000-04-15 00:00:00
abstract::The combination of nerve growth factor (NGF) and phorbol 12-myristate 13-acetate (PMA) rapidly induced the locomotion of PC12 cells by sequentially stimulating lamellar spreading, ruffling with pinocytosis, and polarization by retraction from the substratum. During migration, cells acquired long processes as a result ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19960515)44:4<308::AID-JNR
更新日期:1996-05-15 00:00:00
abstract::Withdrawal of serum from cell cultures constitutes a useful model for the study of mechanisms involved in the regulation of Akt function in vitro. However, there have been several reports of changes in Akt activity that are not fully explained by the current model of phosphatidylinositol 3'-kinase (PI3K)/Akt signaling...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21516
更新日期:2008-02-15 00:00:00
abstract::Migraine is a common neurological disorder with significantly higher incidence and prevalence in women than men. The presentation of the disease in women is modulated by changes in sex hormones from adolescence to pregnancy and menopause. Yet, the effect of sex influences has often been neglected in both basic and cli...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.23903
更新日期:2017-01-02 00:00:00
abstract::Chronic alcohol consumption has adverse effects on the central nervous system, affecting some hippocampal and hypothalamic functions. In this study we tempted to demonstrate that some of these modifications could involve impairment of neurotrophic factors. Three experimental groups of male Sprague Dawley rats were stu...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/1097-4547(20010115)63:2<200::AID-JNR1012>3
更新日期:2001-01-15 00:00:00
abstract::Brain-derived neurotrophic factor (BDNF) is an extensively studied protein that is evolutionarily conserved and widely distributed in the brain of vertebrates. It acts via its cognate receptors TrkB and p75NTR and plays a central role in the developmental neurogenesis, neuronal survival, proliferation, differentiation...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24536
更新日期:2020-05-01 00:00:00
abstract::Isotonic media containing sodium salts from weak organic acids induce cell swelling in several experimental preparations (Grinstein et al., 1984; Jakubovicz et al., 1987). In vivo perfusion of rat dentate gyrus, using a microdialysis probe, with modified Krebs-Ringer bicarbonate solutions in which 50 mM NaCl was isoto...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490260205
更新日期:1990-06-01 00:00:00
abstract::In this study, the responses of nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) and neuronal nitric oxide synthase (nNOS) activities were quantitatively analyzed at different times in both ipsilateral and contralateral sides of trigeminal nuclei, after unilateral trigeminal muscle nerve transection, i...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.1235
更新日期:2001-11-01 00:00:00
abstract::Living organotypic cocultures of rat thalamic and cortical explants were used to examine the effects of blocking action potential activity on the morphological development of axons in the mammalian neocortex. Studies in vivo have suggested that blocking sodium channel-dependent activity influences the growth character...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19960315)43:6<707::AID-JNR
更新日期:1996-03-15 00:00:00
abstract::Immature and mature rat astroglia in culture were assayed for glutamine synthetase (GS) activity after a single exposure to the epileptogen FeCl2. Cells were cultured with both standard and elevated extracellular potassium or glutamate (Glu) concentrations. FeCl2 reduced GS activity below control levels, whereas high ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490240408
更新日期:1989-12-01 00:00:00
abstract::Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that form a subfamily within the steroid hormone receptor group. Recent work has shown that one member of this group, PPARgamma, plays a central role in adipocyte differentiation. As oligodendrocytes are major lipid-producin...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19980301)51:5<563::AID-JNR
更新日期:1998-03-01 00:00:00
abstract::Apoptosis and neuronal atrophy are commonly seen in patients infected with the human immunodeficiency virus type 1 (HIV-1) in the late phase of infection. The HIV-1 envelope glycoprotein gp120 has been suggested to be a causal agent of neuronal loss. Therefore, blocking gp120 neurotoxicity may be an effective way to r...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20780
更新日期:2006-04-01 00:00:00
abstract::The Brn-3a transcription factor is critical for survival and differentiation of sensory neurons derived from neural crest cells (NCC). Interaction of Brn-3a with p53 results in differential effects on target gene expression, which profoundly affects fate of neuronal cells. Here we demonstrate colocalization of p53 in ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20299
更新日期:2004-12-15 00:00:00
abstract::Contacts between horizontal and bipolar cells are described in the retina of the teleost Eugerres plumieri. A single, long expansion observed in the external cone horizontal cells makes contact by means of a terminal button with the cell body of a bipolar. It represents the only contact between this class of horizonta...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490060111
更新日期:1981-01-01 00:00:00
abstract::Neuronal degeneration has been shown to be involved in various neurological disorders. Growth/trophic factors and their receptors are known to be important for the regeneration and survival of neurons. We report here the molecular cloning of a receptor-like protein tyrosine kinase, bsk, (for brain specific kinase). Bs...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490370117
更新日期:1994-01-01 00:00:00
abstract::Two fundamental questions about neuron cell culture were addressed. Can one serum-free medium that was developed for optimum growth of hippocampal neurons support the growth of neurons from other regions of the brain? Is the region specific state of differentiation maintained in culture? To answer these questions, we ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490420510
更新日期:1995-12-01 00:00:00
abstract::Phencyclidine (PCP) is a noncompetitive N-methyl-D-aspartate (NMDA) receptor antagonist. Several studies have demonstrated that chronic NMDA receptor antagonist treatment in humans and animals can cause long-term behavioral changes that are reminiscent of negative and cognitive schizophrenia-like symptoms. The muscari...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21247
更新日期:2007-05-01 00:00:00
abstract::The use of trimethadione (TMO) as a protector in hyperbaric oxygen toxicity in the conscious rat has been examined in detail. The oxidation-reduction state of pyridine nucleotides was measured simultaneously with the EEG activity from the surface of the brain cortex. From the data obtained, a few parameters were calcu...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490010205
更新日期:1975-01-01 00:00:00
abstract::The male preponderance in autism prevalence has brought together the disparate topics of sex/gender and autism research. Two directions of neuroimaging studies on the relationships between sex/gender and autism may inform male-specific risk mechanisms and female-specific protective mechanisms of autism. First, we revi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.23948
更新日期:2017-01-02 00:00:00