Abstract:
:Antibody-producing hybridoma cells specifically bind to microgram quantities of antigen molecules adsorbed onto the surface of plastic microtiter plates. The binding of hybridoma cells to nonantigen is optimally below 5%, similar binding of non-antibody-producing cells is 4-7%, compared to the binding of the hybridomas to their antigen. There is a difference in the kinetics of binding hybridomas to antigen compared to nonantigen. The number of bound cells depends on the amount, i.e., the surface density, of the antigen molecules and shows typical saturation effects. Preincubation of hybridomas with excess free antigen and saturation of the antibody binding site on the surface with the hybridoma-produced antibody reduces binding of the hybridoma cells to the antigen. Treatment of cells with trypsin reduces binding to antigen-coated plastic surfaces. Drugs such as sodium azide, cytochalasin B, colchicine, vinpocetine, and vincristine sulfate reduce binding to the antigen. Hybridoma cells adhering to the antigen produce more antibody than nonadhering cells. The results reported in this paper show that antigen molecules adsorbed to include a plastic surface and hybridoma cells interact specifically. This system forms a suitable model to study the interaction of antigen with antigen-specific cells and may be useful as a separation method for specific antibody-producing cells.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Najbauer J,Tigyi GJ,Nemeth Pdoi
10.1002/jnr.490150312subject
Has Abstractpub_date
1986-01-01 00:00:00pages
415-26issue
3eissn
0360-4012issn
1097-4547journal_volume
15pub_type
杂志文章abstract::Sky (also known as "Tyro3" and "Rse") is a member of the Axl/Sky/Mer receptor tyrosine kinase family and has two immunoglobulin-like repeats and two fibronectin type III-like repeats in the extracellular domain. Gas6 is a ligand for all members of the Axl family, each of which (Axl, Sky, and c-Mer) has different affin...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10211
更新日期:2002-04-15 00:00:00
abstract::The large, medium-sized, and small neurons of the dorsal root ganglion (DRG) have different functions in the processing of various senses. Hyperpolarization-activated, cyclic nucleotide-gated channels (HCN) contribute greatly to neuronal excitability. In the present study, which used whole-cell patch clamp techniques ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20109
更新日期:2004-06-01 00:00:00
abstract::The effects of cations on the spontaneous and potassium-stimulated taurine release from mouse cerebral cortex slices were assessed with an emphasis on the as yet unestablished calcium dependence of the stimulated release. Spontaneous and stimulated GABA release was analyzed for comparison. A depolarizing concentration...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490170316
更新日期:1987-01-01 00:00:00
abstract::Metabolite levels in cerebrospinal fluid from patients with Parkinson disease or Huntington chorea were compared with the levels in healthy controls using proton magnetic resonance spectroscopy. No significant differences were found for any metabolite measured in cerebrospinal fluid from patients with Parkinson diseas...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/1097-4547(20000615)60:6<779::AID-JNR10>3.0
更新日期:2000-06-15 00:00:00
abstract::A cholinergically disrupted laboratory animal has been produced by administration of the cholinotoxin ethylcholine aziridinium mustard (AF64A), which produced a dysfunction in the cholinergic forebrain system. After AF64A treatment, a reduction of choline acetyl transferase (ChAT) activity was measured in the hippocam...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19990115)55:2<178::AID-JNR
更新日期:1999-01-15 00:00:00
abstract::Cultured astrocytes from neonatal rat cerebral hemispheres are depolarized by the excitatory neurotransmitter glutamate. In this study we have used selective agonists of different neuronal glutamate receptor subtypes, namely, the N-methyl-D-aspartate (NMDA), kainate, and quisqualate type, to characterize pharmacologic...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490220307
更新日期:1989-03-01 00:00:00
abstract::Gangliosides, sialic acid-containing glycosphingolipids, are ubiquitously expressed in all eukaryotic cells and are localized primarily in the plasma membrane. For a rat pheochromocytoma cell line, PC12, which has been used frequently as a model for investigating events leading to neuronal differentiation, it is gener...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21031
更新日期:2006-11-01 00:00:00
abstract::Noradrenalin (NA) regulates the expression of arginine-vasopressin (AVP) and oxytocin (OT) by magnocellular neurons in the supraoptic nucleus (SON) of the hypothamalus. Nitric oxide (NO) may be one of the factors involved in the NA signaling pathway regulating AVP and OT expression. To test this possibility, we used a...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21394
更新日期:2007-10-01 00:00:00
abstract::Increasing evidence has shown that β-amyloid (Aβ) induces hyperphosphorylation of tau and contributes to Aβ toxicity. Recently, tau hyperphosphorylation by glycogen synthase kinase-3β (GSK-3β) activation has been emphasized as one of the pathogenic mechanisms of Alzheimer's disease (AD). The phosphoinositide 3 kinase ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22563
更新日期:2011-03-01 00:00:00
abstract::Unilateral eye removal results in an increased delivery of blood-borne glucose, norepinephrine, choline, and tyrosine, but not of proline, to the denervated optic lobes. Administration of reserpine to chicks increases penetrance of norepinephrine into the brain but not of tyrosine, choline, or proline. Selective alter...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490010308
更新日期:1975-01-01 00:00:00
abstract::Anorexia nervosa (AN) is a severe and complex psychiatric disorder characterized by intense fear about weight gain and finalized to food-related control behaviors. Growing interest has been demonstrated about neurobiological processes subtend to AN physiopathology. The present review aimed to collect neurostructural a...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24674
更新日期:2020-11-01 00:00:00
abstract::The rationale for designing and synthesizing 24 different peptide analogs and subpeptides of residues 69-84 of guinea pig myelin basic protein is presented. The encephalitogenic potential of 19 of these peptides in Lewis rats is also given. ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490180130
更新日期:1987-01-01 00:00:00
abstract::Individuals who regularly shift their sleep timing, like night and/or shift-workers suffer from circadian desynchrony and are at risk of developing cardiometabolic diseases and cancer. Also, shift-work is are suggested to be a risk factor for the development of mood disorders such as the burn out syndrome, anxiety, an...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.24741
更新日期:2021-02-01 00:00:00
abstract::Comparisons were made of whole cell voltage clamp recordings from cultures of normal Schwann cells (SC) from three human subjects and from three neurofibrosarcoma cell lines. The whole cell K+ (K) currents of normal and tumor cells could be divided into three types based on voltage activation range, pharmacology, and ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19981115)54:4<495::AID-JNR
更新日期:1998-11-15 00:00:00
abstract::Neural precursor cells, including neural stem and progenitor cells, in the subventricular zone (SVZ) are the main targets for cytomegalovirus (CMV) infection in developing brains. The neural precursor cells in the SVZ of the adult brain have been reported to respond by proliferating after infusion with epidermal growt...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21410
更新日期:2007-10-01 00:00:00
abstract::Antiglycolipid IgM antibodies are known to induce formation of "wide spaced" or "expanded" myelin, a distinctive form of dysmyelination characterized by a repeat period approximately two or three times normal, which is seen also in diseases, including multiple sclerosis. To determine whether an antibody directed again...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21914
更新日期:2009-03-01 00:00:00
abstract::Physiological responses to acute stress proceed with the activation of the hypothalamus-pituitary-adrenal gland (HPA) system. Many brain regions are known to modulate the HPA axis activation in stress responses, but the detailed neural circuits and signaling system in the upstream of the HPA axis have to be explored f...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22119
更新日期:2009-10-01 00:00:00
abstract::We have recently demonstrated that brain plastic events significantly modify synaptic protein synthesis measured by the incorporation of [(35)S]methionine in brain synaptosomal proteins. Notably, in rats learning a two-way active avoidance task, the local synthesis of two synaptic proteins was selectively enhanced. Be...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22037
更新日期:2009-07-01 00:00:00
abstract::Acute intoxication with large ammonia doses leads to activation of NMDA receptors in the brain, resulting in oxidative stress and disturbance of mitochondrial function. Altered mitochondrial function is a crucial step in some mechanisms of cellular apoptosis. This study assesses whether ammonia intoxication in vivo le...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21385
更新日期:2007-08-15 00:00:00
abstract::Previous studies from our laboratory suggest that Alzheimer's disease sera contain a repertoire of antibodies to the heavy neurofilament subunit (NF-H) and that a subpopulation of these antibodies bind specifically to epitopes highly enriched in NF-H isolated from the purely cholinergic electromotor neurons of Torpedo...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490290409
更新日期:1991-08-01 00:00:00
abstract::The blood-brain barrier (BBB) refers to the network of microvessels that selectively restricts the passage of substances between the circulation and the central nervous system (CNS). This microvascular network is comprised of arterioles, capillaries and venules, yet the respective contribution of each of these to the ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22316
更新日期:2010-05-15 00:00:00
abstract::Ischemia elicits a variety of stress responses in neuronal cells, which result in cell death. wld(S) Mice bear a mutation that significantly delays Wallerian degeneration. This mutation also protects all neuronal cells against other types of stresses resulting in cell death, including ischemia. To clarify the types of...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22792
更新日期:2012-03-01 00:00:00
abstract::Evidence indicates that the ubiquitin-proteasome system and the endoplasmic retculum (ER) quality-control system work in concert to ensure that proteins are correctly folded in the ER and that misfolded proteins are retrotransported to the cytosol for degradation by proteasomes. Dysfunction of either system results in...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23413
更新日期:2014-10-01 00:00:00
abstract::The cutaneous electroreceptor "tuberous organs" of the lateral line system of Eigenmannia virescens were studied at light and electron microscopic levels with immunohistochemical and autoradiographic techniques after sectioning of the posterior branch of the lateral line nerve. After deafferentation total degeneration...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490390506
更新日期:1994-12-01 00:00:00
abstract::Long-duration exposure to weightlessness results in bone demineralization, muscle atrophy, cardiovascular deconditioning, altered sensory-motor control, and central nervous system reorganizations. Exercise countermeasures and body loading methods so far employed have failed to prevent these changes. A human mission to...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/1097-4547(20001015)62:2<169::AID-JNR2>3.0.
更新日期:2000-10-15 00:00:00
abstract::Structure and dimension of the dendritic arbor are important determinants of information processing by the nerve cell, but mechanisms and molecules involved in dendritic growth are essentially unknown. We investigated early mechanisms of dendritic growth using mouse fetal hippocampal neurons in primary culture, which ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19990601)56:5<539::AID-JNR
更新日期:1999-06-01 00:00:00
abstract::Granulocyte colony-stimulating factor (G-CSF) is a hematopoietic cytokine that also possesses neurotrophic and antiapoptotic properties. G-CSF has been reported to decrease amyloid burden significantly, promote hippocampal neurogenesis, and improve spatial learning in a mouse model of Alzheimer's disease. To understan...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23378
更新日期:2014-08-01 00:00:00
abstract::We reported previously that stem cell factor (SCF) is produced mainly by neurons and that its receptor (c-kitR), encoded by the protooncogene c-kit, is expressed in microglia, suggesting that SCF/c-kitR signaling may be involved in neuron-microglia interactions. We now report that SCF supports microglial survival in c...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19980701)53:1<29::AID-JNR4
更新日期:1998-07-01 00:00:00
abstract::Plexiform layer synaptic and photoreceptor cell components were investigated in retinas of Royal College of Surgeons (RCS) dystrophic rats transplanted with normal retinal pigment epithelial (RPE) cells by immunocytochemistry using previously characterized monoclonal antibodies. In retinas of normal adult rats and RPE...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490360408
更新日期:1993-11-01 00:00:00
abstract::A link between aluminum (Al) exposure and age-related neurological disorders has long been proposed. Although the exact mechanism by which the metal may influence disease processes is unknown, there is evidence that exposure to Al causes an increase in both oxidative stress and inflammatory events. These processes hav...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10877
更新日期:2004-02-15 00:00:00