Abstract:
:Neural precursor cells, including neural stem and progenitor cells, in the subventricular zone (SVZ) are the main targets for cytomegalovirus (CMV) infection in developing brains. The neural precursor cells in the SVZ of the adult brain have been reported to respond by proliferating after infusion with epidermal growth factor (EGF). Here we report the susceptibility of the precursor cells in the adult mouse brain to murine CMV (MCMV) infection. Adult mouse brains from 10-, 25-, and 70-week-old (W) mice were infused with either phosphate-buffered saline or EGF into the brain for 3 days, and then intracerebrally infected with MCMV for 5 days. The susceptibility of the adult brains to MCMV was significantly increased by infusion of EGF in terms of viral titers and viral antigen-positive cells. The susceptibility of the young adult brain from 10-week-old mice to MCMV was higher than that of the adult brains from 25-week-old or 70-week-old mice. Both the ependymal and the SVZ cells were susceptible to MCMV infection. The number of virus-infected cells in the SVZ was significantly increased by infusion of EGF, whereas the number of infected ependymal cells was not significantly increased. Among the virus-infected cells in the SVZ, 73% were positive for nestin, 87% were positive for Musashi, 86% were positive for GFAP, and 96% were positive for PCNA. These results indicate that the susceptibility of the adult brain to MCMV is correlated with the proliferative ability of the neural precursor cells in the SVZ of the adult brain.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Han GP,Li L,Kosugi I,Kawasaki H,Tsuchida T,Miura K,Tsutsui Ydoi
10.1002/jnr.21410subject
Has Abstractpub_date
2007-10-01 00:00:00pages
2981-90issue
13eissn
0360-4012issn
1097-4547journal_volume
85pub_type
杂志文章abstract::Cathepsin D-like activity in homogenates of five brain areas of 3-month-old and 24-month-old Fischer 344 rats was measured. With hemoglobin as substrate at pH 3.2, more than 90% of the activity was inhibited by pepstatin. In each area studied, activity was more than twice as high in the old rat brain: 140-160% higher ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490230412
更新日期:1989-08-01 00:00:00
abstract::Few diseases in clinical medicine cause as much diagnostic consternation as central nervous system (CNS) vasculitis because of its varying modes of presentation and frequently overlapping clinical and pathological features. There are no pathognomonic clinical or laboratory findings. The purpose of the present retrospe...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10180
更新日期:2002-03-15 00:00:00
abstract::Both protein kinase C (PKC) and ceramide play a critical role in cell signaling, but the relationship between PKC and ceramide is unclear. Low concentrations of ceramide were observed to transiently stimulate PKC zeta activity in vitro and in vivo, whereas high doses of ceramide lead to inhibition of PKC zeta. Inhibit...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19990201)55:3<293::AID-JNR
更新日期:1999-02-01 00:00:00
abstract::Pigment epithelium-derived factor (PEDF) is a neurotrophic protein synthesized and secreted by retinal pigment epithelial (RPE) cells in early embryogenesis and has been shown to be present in the extracellular matrix between the RPE cells and the neural retina. It induces neuronal differentiation and promotes surviva...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:1999-09-15 00:00:00
abstract::Recent advances in human induced pluripotent stem cells (hiPSCs) offer new possibilities for biomedical research and clinical applications. Differentiated neurons from hiPSCs are expected to be useful for developing novel methods of treatment for various neurological diseases. However, the detailed process of function...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23666
更新日期:2015-12-01 00:00:00
abstract::The neural cell adhesion molecule (NCAM) plays a key role in neural development, regeneration, and synaptic plasticity. The crystal structure of a fragment of NCAM comprising the three N-terminal immunoglobulin (Ig)-like modules indicates that the first and second Ig modules bind to each other, thereby presumably medi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22380
更新日期:2010-08-01 00:00:00
abstract::This study aimed at determining, based on independent predictors of taste intensity and emotional response, whether individual personality traits could affect prediction models of overall liking and preference rank toward basic taste solutions. Sixty-seven participants rated taste intensities (TI) of four basic-taste ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.24321
更新日期:2019-03-01 00:00:00
abstract::The chemokines, macrophage inflammatory protein-1 (MIP-1) and its subunit MIP-1 beta, induce an intense fever in the rat when they are injected directly into the anterior hypothalamic, pre-optic area (AH/POA), a region containing thermosensitive neurons. The purpose of this study was to compare the central action on b...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490390105
更新日期:1994-09-01 00:00:00
abstract::The discharge behavior of neurons depends on a variable expression and sorting pattern of voltage-dependent potassium (Kv) channels that changes during development. The rodent retina represents a neuronal network whose main functions develop after birth. To obtain information about neuronal maturation we analyzed the ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21105
更新日期:2007-01-01 00:00:00
abstract::High levels of the proto-oncogene product, pp60c-src, have been found in developing and adult neural tissues as well as in certain fully mature cells of the hematopoietic lineage, e.g., platelets and myelomonocytes. Adrenal medullary chromaffin cells exhibit characteristics of both types of cells, i.e., they are deriv...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490240107
更新日期:1989-09-01 00:00:00
abstract::The extension of filopodia from growth cones of regenerating neurites from rat superior cervical ganglion neurons in tissue culture was studied. Cultures were grown on a thin layer of fibrous collagen and maintained in a medium containing serum and nerve growth factor. Time-lapse cinematography and computer-assisted m...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490130111
更新日期:1985-01-01 00:00:00
abstract::Alterations in the 5-HT2A receptor gene expression in the prefrontal cortex have been suggested to play a role in the pathophysiology and pharmacotherapy of schizophrenia. This study measured mRNA encoding 5-HT2A receptor in the left superior frontal gyrus from chronic elderly schizophrenics (n = 21) with varying neur...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:
更新日期:2000-01-15 00:00:00
abstract::A series of AMPA [(R,S)-alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid] analogues were evaluated for activity at homomeric, heteromeric, and chimeric rat GluR1(o) and GluR3(o) receptors expressed in Xenopus oocytes, using the two-electrode voltage clamp technique. The formation of heteromeric receptor compl...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(sici)1097-4547(19970715)49:2<176::aid-jnr
更新日期:1997-07-15 00:00:00
abstract::Cerebrospinal fluid was collected from the lateral ventricles and cisternal space from beagle dogs under light pentobarbital anesthesia. Homovanillic acid (HVA), 5-hydroxyindoleacetic acid (5-HIAA), and 3-methoxy-4-hydroxyphenylglyco-sulfate (MOPEG-SO4) levels in the liquor taken from these two regions were evaluated,...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490010513
更新日期:1975-01-01 00:00:00
abstract::Corticosterone (CORT), one of the glucocorticoids, causes neuronal damage in the hippocampus, but the mechanism(s) of action underlying its effects remains unknown. Brain-derived neurotrophic factor (BDNF) is a neurotrophic factor that belongs to the neurotrophin family, affects the survival and/or differentiation of ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19990715)57:2<227::AID-JNR
更新日期:1999-07-15 00:00:00
abstract::Glial cell-line derived neurotrophic factor (GDNF) is the most potent trophic factor for motoneuron survival and neuromuscular junction formation. GDNF is upregulated in injured or denervated skeletal muscle and returns to normal levels following reinnervation. However, the mechanism by which GDNF is regulated in dene...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23512
更新日期:2015-03-01 00:00:00
abstract::Adenosine signalling has an important role in cochlear protection from oxidative stress. In most tissues, intracellular adenosine kinase (ADK) is the primary route of adenosine metabolism and the key regulator of intracellular and extracellular adenosine levels. The present study provides the first evidence for ADK di...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22421
更新日期:2010-09-01 00:00:00
abstract::Factor Xa (FXa) plays a critical role in the coagulation cascade by generation of thrombin. During focal ischemia thrombin levels increase in the brain tissue and cause neural damage. This study examined the hypothesis that administration of the FXa inhibitor, apixaban, following focal ischemic stroke may have therape...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.24253
更新日期:2018-08-01 00:00:00
abstract::Several neuronal and non-neuronal cell lines express a Ca(2+)-dependent mechanism of transmitter release that can be demonstrated after loading the cells with acetylcholine during culture. In contrast, a particular cell line, the neuroblastoma N18TG-2, was found to be deficient for release. We transfected N18TG-2 cell...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(SICI)1097-4547(19960801)45:3<195::AID-JNR
更新日期:1996-08-01 00:00:00
abstract::The vesicular acetylcholine transporter (VAChT) is a transmembrane protein required, in cholinergic neurons, for selective storage of acetylcholine into synaptic vesicles. Although dorsal root ganglion (DRG) neurons utilize neuropeptides and amino acids for neurotransmission, we have previously demonstrated the presen...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10855
更新日期:2004-01-15 00:00:00
abstract::Synaptic vesicles were isolated from adult bovine cortical gray matter by differential centrifugation and membrane filtration of a hypoosmotically lysed crude mitochondrial fraction. Vesicle preparations were analyzed for purity by electron microscopy and enzyme assays. Polyacrylamide gel electrophoresis of SDS-solubi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490030204
更新日期:1977-01-01 00:00:00
abstract::Soluble amyloid beta protein (Abeta) oligomers have been considered recently to be responsible for the cognitive dysfunction that sets in prior to senile plaque formation in the Alzheimer's disease (AD) brain. By using the newly prepared antibody against oligomer Abeta, rather than fibrillar or monomer Abeta, we obser...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20952
更新日期:2006-08-15 00:00:00
abstract::Human immunodeficiency virus type 1 (HIV-1) infection in its human host often results in progressive dementia and encephalopathy in adults and children, respectively. The mechanisms underlying virus-induced neurocognitive dysfunction are not fully understood. However, several studies strongly suggest that secretory vi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20745
更新日期:2006-02-15 00:00:00
abstract::The brainstem nucleus of the solitary tract (NTS) is the key integrating relay in the central processing of sensory information from the thoracic and from most subdiaphragmatic viscera. Modulation of neuronal excitability and synaptic activity in the NTS by nicotinic agents can have potent effects on vital physiologic...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23436
更新日期:2014-11-01 00:00:00
abstract::The hypoxia-responsive cytokine erythropoietin (EPO) provides neuroprotective effects in the damaged brain during ischemic events and neurodegenerative diseases. The purpose of the present study is to evaluate the EPO/EPO receptor (EPOR) endogenous system between astrocyte and oligodendrocyte precursor cell (OPC) unde...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22702
更新日期:2011-10-01 00:00:00
abstract::Previously, we have demonstrated a novel interaction between Galpha(o) protein and Purkinje cell protein-2 (Pcp2, also known as L7) in vitro and in transfected cells (Luo and Denker [1999] J. Biol. Chem. 274:10685-10688). Pcp2 is uniquely expressed in cerebellar Purkinje cells and in retinal bipolar neurons, and it ma...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10460
更新日期:2002-12-01 00:00:00
abstract::This Review describes some in vitro approaches used to investigate the mechanisms involved in Krabbe's disease, with particular regard to the cellular systems employed to study processes of inflammation, apoptosis, and angiogenesis. The aim was to update the knowledge on the results obtained from in vitro models of th...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.23846
更新日期:2016-11-01 00:00:00
abstract::By using two different approaches, ubiquitin C-terminal hydrolase 1 (UCHL1) was identified as a potential cerebrospinal fluid (CSF) biomarker of neuronal loss in aneurysmal subarachnoid hemorrhage (ASAH) and presumably other CNS damage and disease states. Appropriate antibodies and a sensitive ELISA were generated, an...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.22323
更新日期:2010-05-15 00:00:00
abstract::To elucidate the biological differences in neural phenotype between malignant rhabdoid tumor (MRT) and neuroblastoma cell lines, we examined the expression of solube N-ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) complex proteins in MRT cell lines under differentiation induction with 12-...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10330
更新日期:2002-09-01 00:00:00
abstract::The transforming growth factors beta (TGF-beta), a family of regulatory polypeptides, are involved in numerous vital processes including inflammation and wound healing. Since repair of a peripheral nerve lesion includes a series of well-defined steps of cellular actions possibly controlled by TGF-beta s, and since TGF...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490390408
更新日期:1994-11-01 00:00:00