Abstract：
:In this report, we address the molecular mechanisms that regulate axonal growth by focusing on the gene for one of the major axonal cytoskeletal proteins, T alpha1 alpha-tubulin. During the developmental growth of sympathetic neurons, transcription of a beta-galactosidase transgene driven by the T alpha1 promoter (T alpha1:nlacZ) was high until the time of target innervation and neuronal maturation, when it decreased significantly. In mature animals, T alpha1:nlacZ transcription remained relatively low until target contact was experimentally disrupted; when facial motoneurons were axotomized, T alpha1:nlacZ transgene expression increased, was maximal for 1-7 days, and, if neurons regenerated and reinnervated their target musculature, returned to control levels by 49 days. In contrast, if regeneration and reestablishment of target contact were inhibited, transgene expression remained elevated. To determine whether this increased transcription was due to the loss of target contact or to axonal loss, we transected sympathetic neurons that project to the eye either close to or far from their cell bodies. In both cases, when target contact was severed, T alpha1:nlacZ transcription increased. These experiments indicate that transcription of the T alpha1 alpha-tubulin promoter is repressed by target contact in both developing and mature neurons. We suggest that this repression is due to a target-derived "stop-growth" factor that retrogradely signals to regulate transcription of this and other genes that are required for axonal growth.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Wu W,Gloster A,Miller FDsubject
Has Abstractpub_date
1997-06-01 00:00:00pages
477-87issue
5eissn
0360-4012issn
1097-4547pii
10.1002/(SICI)1097-4547(19970601)48:5<477::AID-JNRjournal_volume
48pub_type
杂志文章abstract：:Ex vivo gene therapy is emerging as a promising approach for the treatment of neurodegenerative diseases and central nervous system (CNS) trauma. We have shown previously that human adult astrocytes can be expanded in vitro and can express various therapeutic transgenes (Ridet et al. [1999] Hum. Gene Ther. 10:271-280;...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10617
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abstract：:Transplanting neural stem cells (NSC) to the damaged brain has been regarded as a potential treatment for neurodegenerative diseases such as Alzheimer's disease (AD), a condition characterized by memory loss. We hypothesized that transplantation of NSC into the hippocampal regions of APP + PS1 transgenic (Tg) mice, a ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23299
更新日期：2014-02-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21131
更新日期：2007-02-01 00:00:00
abstract：:An immortalized dorsal root ganglion cell line F-11 exhibits many properties of spinal cord neurons and undergoes apoptosis in response to growth factor withdrawal and the exogenous addition of inhibitors of phosphatidylinositol-3-kinase (PI3K). To elucidate the mechanism of apoptosis we generated F-11 clones which ov...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：
更新日期：1999-09-15 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490010206
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490200411
更新日期：1988-08-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490270210
更新日期：1990-10-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(sici)1097-4547(19970801)49:3<389::aid-jnr
更新日期：1997-08-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20827
更新日期：2006-05-15 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10155
更新日期：2002-03-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490370512
更新日期：1994-04-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21563
更新日期：2008-04-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：
更新日期：1997-04-15 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490260314
更新日期：1990-07-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/1097-4547(20010101)63:1<20::AID-JNR3>3.0.C
更新日期：2001-01-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：
更新日期：1999-11-15 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22582
更新日期：2011-04-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20016
更新日期：2004-04-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490220414
更新日期：1989-04-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(20000501)60:3<284::AID-JNR
更新日期：2000-05-01 00:00:00
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journal_title：Journal of neuroscience research
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doi：10.1002/jnr.21984
更新日期：2009-11-15 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20518
更新日期：2005-08-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.24605
更新日期：2021-01-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19981101)54:3<353::AID-JNR
更新日期：1998-11-01 00:00:00
abstract：:Injury to the central nervous system (CNS) either from trauma or due to demyelinating/degenerating diseases results in a typical response of astrocytes, termed astrogliosis. This reaction is characterized by astrocyte proliferation, extensive hypertrophy of nuclei, cell body, and cytoplasmic processes and an increase ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490380403
更新日期：1994-07-01 00:00:00
abstract：:A frontal pole cDNA library from monkey (Macaca mulatta) brain was screened to identify mRNAs that are expressed more in frontal pole as compared to primary visual cortex. Three cDNA clones, whose greater expression was confirmed by Northern blot analysis, were identified as cytochrome oxidase (COX) subunits I, II, an...
journal_title：Journal of neuroscience research
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更新日期：1992-07-01 00:00:00
