Abstract:
:The demyelinative potential of the cytokines interleukin-1 alpha (IL-1 alpha), interferon-gamma (IFN-gamma), and tumor necrosis factor-alpha (TNF-alpha) has been investigated in myelinating aggregate brain cell cultures. Treatment of myelinated cultures with these cytokines resulted in a reduction in myelin basic protein (MBP) content. This effect was additively increased by anti-myelin/oligodendrocyte glycoprotein (alpha-MOG) in the presence of complement. Qualitative immunocytochemistry demonstrated that peritoneal macrophages, added to the fetal telencephalon cell suspensions at the start of the culture period, successfully integrated into aggregate cultures. Supplementing the macrophage component of the cultures in this fashion resulted in increased accumulation of MBP. The effect of IFN-gamma on MBP content of cultures was not affected by the presence of macrophages in increased numbers.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Loughlin AJ,Honegger P,Woodroofe MN,Comte V,Matthieu JM,Cuzner MLdoi
10.1002/jnr.490370512subject
Has Abstractpub_date
1994-04-01 00:00:00pages
647-53issue
5eissn
0360-4012issn
1097-4547journal_volume
37pub_type
杂志文章abstract::Antibody-producing hybridoma cells specifically bind to microgram quantities of antigen molecules adsorbed onto the surface of plastic microtiter plates. The binding of hybridoma cells to nonantigen is optimally below 5%, similar binding of non-antibody-producing cells is 4-7%, compared to the binding of the hybridoma...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490150312
更新日期:1986-01-01 00:00:00
abstract::Neuronal progenitors in the adult hippocampus continually proliferate and differentiate to the neuronal lineage, and ischemic insult promotes hippocampal neurogenesis. However, newborn neurons show a progressive reduction in numbers during the initial few weeks, therefore, enhanced survival of newborn neurons seems to...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
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更新日期:2006-11-01 00:00:00
abstract::In offspring, an adequate maternal diet is important for neurodevelopment. One mechanism by which maternal diet impacts neurodevelopment is through its dynamic role in the development of the gut microbiota. Communication between the gut, and its associated microbiota, and the brain is facilitated by the vagus nerve, i...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.24605
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abstract::The present study examined trophic activities in normal and injured brain which affect the survival and growth of central neurons in culture. Adult rats received bilateral lesions through the angular bundle, severing projections between the entorhinal cortex and the hippocampus. Ten days later, extracts were prepared ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490180203
更新日期:1987-01-01 00:00:00
abstract::Huntington's disease (HD) is a progressive neurodegenerative disorder caused by an expanded CAG repeat within the huntingtin (HTT) gene. The Q140 and HdhQ150 knock-in HD mouse models were generated such that HdhQ150 mice have an expanded CAG repeat inserted into the mouse Htt gene, whereas in the Q140s, mouse exon 1 H...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.24493
更新日期:2019-12-01 00:00:00
abstract::Heterothermic mammals such as ground squirrels tolerate ischemia and N-methyl-D-aspartate (NMDA) better than homeothermic mammals such as rats both in vivo and in vitro, and this tolerance is enhanced in the hibernating state. However, the cellular mechanisms underlying this tolerance remain unclear. NMDA receptors (N...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20893
更新日期:2006-08-01 00:00:00
abstract::Thymocyte differentiation antigen-1 (Thy-1) is a cell surface glycoprotein found on T cells and neurons and is involved in cell-to-cell interactions. In addition, Thy-1 knockouts (KO) are a potential mouse model of restless legs syndrome (RLS) based on clinical observations and the role of dopamine in the disease. In ...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23046
更新日期:2012-08-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(sici)1097-4547(19990415)56:2<213::aid-jnr
更新日期:1999-04-15 00:00:00
abstract::Cerebrospinal fluid was collected from the lateral ventricles and cisternal space from beagle dogs under light pentobarbital anesthesia. Homovanillic acid (HVA), 5-hydroxyindoleacetic acid (5-HIAA), and 3-methoxy-4-hydroxyphenylglyco-sulfate (MOPEG-SO4) levels in the liquor taken from these two regions were evaluated,...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490010513
更新日期:1975-01-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21070
更新日期:2006-12-01 00:00:00
abstract::The expression of different muscarinic receptor subtypes was analyzed in immature Schwann cells obtained from sciatic nerve of 2-day neonatal rats. By using RT-PCR analysis, we demonstrated the presence of M1, M2, M3, and M4 receptor subtypes in cultured Schwann cells, with M2 displaying the highest expression levels....
journal_title:Journal of neuroscience research
pub_type: 杂志文章
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更新日期:2006-07-01 00:00:00
abstract::Culturing sympathetic ganglion neurons in vitro may modify phenotypic expression of some neurotransmitters. For dorsal root ganglia (DRG), contradictory results have been reported; most studies have used immature material. We have therefore performed a detailed immunocytochemical analysis of the transmitter content of...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490220414
更新日期:1989-04-01 00:00:00
abstract::A series of AMPA [(R,S)-alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid] analogues were evaluated for activity at homomeric, heteromeric, and chimeric rat GluR1(o) and GluR3(o) receptors expressed in Xenopus oocytes, using the two-electrode voltage clamp technique. The formation of heteromeric receptor compl...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/(sici)1097-4547(19970715)49:2<176::aid-jnr
更新日期:1997-07-15 00:00:00
abstract::Asphyxia during delivery produces long-term deficits in brain development, including hippocampus. We investigated hippocampal plasticity after perinatal asphyxia, measuring postnatal apoptosis and neurogenesis. Asphyxia was performed by immersing rat fetuses with uterine horns removed from ready-to-deliver rats into a...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21715
更新日期:2008-09-01 00:00:00
abstract::Neuronal cell differentiation alterations induced by mutant presenilin 2 (PS2) were investigated in transgenic mice expressing wild-type or mutant-type PS2. Progressive increases in differentiation and marker protein expression were found in neuronal cells expressing wild-type PS2, whereas these processes were much pe...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.20655
更新日期:2005-11-15 00:00:00
abstract::The effects of blinding with or without pineal ablation on brain monoamine levels were studied in male rats. Brain dopamine (DA), norepinephrine (NE), epinephrine (E), and serotonin (5-HT) were measured by radioenzymatic assays. Four weeks following pinealectomy, E levels were significantly enhanced in the frontal cor...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490100107
更新日期:1983-01-01 00:00:00
abstract::Neuronal degeneration has been shown to be involved in various neurological disorders. Growth/trophic factors and their receptors are known to be important for the regeneration and survival of neurons. We report here the molecular cloning of a receptor-like protein tyrosine kinase, bsk, (for brain specific kinase). Bs...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490370117
更新日期:1994-01-01 00:00:00
abstract::Nociceptive neuronal activation in subcortical regions has not been well investigated in functional magnetic resonance imaging (fMRI) studies. The present report aimed to use the blood oxygenation level-dependent (BOLD) fMRI technique to map nociceptive responses in both subcortical and cortical regions by employing a...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21638
更新日期:2008-06-01 00:00:00
abstract::Oligodendrocytes have a high rate of synthetic activity and produce vast amounts of myelin. The membrane production requires specific sorting and transport processes and structural support. In culture, oligodendrocytes extend flat membranous sheets containing an extensive cytoskeletal network of microtubules (MTs) and...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/(sici)1097-4547(20000101)59:1<11::aid-jnr2
更新日期:2000-01-01 00:00:00
abstract::We studied the postischemic alterations of second messenger and receptor systems focusing on the strionigral pathway in order to clarify the mechanism of the delayed neuronal changes in remote areas of the rat brain after transient focal ischemia. Chronological changes of [3H]forskolin and [3H]SCH 23390 binding sites ...
journal_title:Journal of neuroscience research
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更新日期:1992-11-01 00:00:00
abstract::The changes in ganglioside composition and metabolism of deafferentiated rat hippocampus were estimated after septal lesion. A significant decrease in total ganglioside concentration was found 7 days after the lesion. The reduced level of total gangliosides persisted at 17 and 25 days. Relative increase in the proport...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490210107
更新日期:1988-09-01 00:00:00
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journal_title:Journal of neuroscience research
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更新日期:2000-05-01 00:00:00
abstract::Despite consistent observations of sex differences in depression and related emotional disorders, we do not yet know how these sex differences modulate the effects of genetic polymorphisms implicated in risk for these disorders. This Mini-Review focuses on genetic polymorphisms of the serotonergic system to illustrate...
journal_title:Journal of neuroscience research
pub_type: 杂志文章,评审
doi:10.1002/jnr.23877
更新日期:2017-01-02 00:00:00
abstract::The transplantation of dopaminergic (DA) progenitors derived from pluripotent stem cells improves the behavior of Parkinson's disease model animals. However, the survival of DA progenitors is low, and the final yield of DA neurons is only approximately 0.3%-2% the number of transplanted cells. Zonisamide (ZNS) increas...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.24668
更新日期:2020-08-01 00:00:00
abstract::Heat shock protein 60 (HSP60) and HSP10 are mitochondrial chaperonin proteins and are responsible for the folding of newly imported proteins and 13 polypeptides encoded by mitochondrial DNA. The expressions of HSP60 and HSP10 are regulated simultaneously because these genes are localized head to head on chromosome, se...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21236
更新日期:2007-05-01 00:00:00
abstract::To examine the role of platelet-derived growth factor (PDGF) in the in vivo regulation of Schwann cell proliferation, steady-state levels of mRNAs encoding PDGF A and B chains, and PDGF alpha and beta receptors were measured in immature and adult rat sciatic nerves and in cultured rat Schwann cells. PDGF B chain and P...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490310206
更新日期:1992-02-01 00:00:00
abstract::The development of cells of the oligodendroglial lineage, from immature progenitor to myelinating cells, occurs largely in a neuronal environment, yet little is known about specific interactions between these 2 cell types. We have tested the effects of medium conditioned by cultures of rat cerebral cortical neurons (C...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490340510
更新日期:1993-04-01 00:00:00
abstract::Abdominal muscle electromyograms were monitored in response to graded mechanical loads added to either inspiration or expiration in anesthetized cats. The surgical preparation, the apparatus, and the levels of loads applied (resistive, tracheal occlusion, and continuous positive pressure) were matched with those emplo...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490130413
更新日期:1985-01-01 00:00:00
abstract::The contribution of glucose carbon to the biosynthesis of cholesterol and phospholipids in distinct brain regions was studied quantitatively in the adult male rat. Rates of flow of glucose carbon into the lipids in vivo were calculated from two measurements: the curve representing the decrease in plasma 14C-glucose wi...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490060505
更新日期:1981-01-01 00:00:00
abstract::The mutant hypogonadal (hpg) mouse lacks a functioning gene for the neurohormone gonadotropin releasing hormone (GnRH). Previous studies from our laboratory had indicated that the initiation and maintenance of reproductive function in these mice could be brought about by the implantation of normal fetal grafts into ad...
journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490310121
更新日期:1992-01-01 00:00:00