Attempts to produce astrocyte cultures devoid of oligodendrocyte generating potential by the use of antimitotic treatment reveal the presence of quiescent oligodendrocyte precursors.

Abstract:

:The presence of oligodendrocyte precursor cells which cannot be removed from primary cultures by antibody-dependent techniques complicates the interpretation of transplantation experiments designed to examine the potential of astrocytes to influence remyelination (Blakemore et al.; Glia 13:79-91, 1995). In the present series of experiments we have investigated the use of the antimitotic cytosine arabinoside to eliminate oligodendrocyte precursors from mixed glial cell cultures following immunolytic removal of both oligodendrocytes and oligodendrocyte progenitors using A2B5 and O4 monoclonal antibodies. Our results indicate that not all oligodendrocyte precursors are involved during the subsequent regeneration of oligodendrocytes since a population of precursors survive 3-day and 12-day exposure to cytosine arabinoside. Maintaining immunolysed cultures in serum-free medium containing cytosine arabinoside for 23 days, removed the potential to generate large clones of oligodendrocytes both in vitro and following transplantation. However, a small number of oligodendrocyte precursors survived this treatment and generated single oligodendrocytes in vitro and isolated clusters of oligodendrocyte remyelination following transplantation. Overall, these results indicate that oligodendrocyte precursors have considerable potential to generate oligodendrocytes, but, since they can also survive for long periods in a quiescent state, their complete elimination from immunolysed astrocyte cultures by the use of an antimitotic is unreliable, if not impossible.

journal_name

J Neurosci Res

authors

Crang AJ,Blakemore WF

subject

Has Abstract

pub_date

1997-07-01 00:00:00

pages

64-71

issue

1

eissn

0360-4012

issn

1097-4547

pii

10.1002/(SICI)1097-4547(19970701)49:1<64::AID-JNR7

journal_volume

49

pub_type

杂志文章
  • TorsinA in PC12 cells: localization in the endoplasmic reticulum and response to stress.

    abstract::Most cases of early-onset torsion dystonia are caused by deletion of GAG in the coding region of the DYT1 gene encoding torsinA. This autosomal dominant neurologic disorder is characterized by abnormal movements, believed to originate from neuronal dysfunction in the basal ganglia of the human brain. The torsins (tors...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.10567

    authors: Hewett J,Ziefer P,Bergeron D,Naismith T,Boston H,Slater D,Wilbur J,Schuback D,Kamm C,Smith N,Camp S,Ozelius LJ,Ramesh V,Hanson PI,Breakefield XO

    更新日期:2003-04-15 00:00:00

  • Inflammatory markers in women with postpartum depressive symptoms.

    abstract::Postpartum depression (PPD) is a devastating disorder affecting not only more than 10% of all women giving birth, but also the baby, the family, and the society. Compiling evidence suggests the involvement of the immune system in the pathophysiology of major depression; yet, the immune response in perinatal depression...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.24312

    authors: Bränn E,Fransson E,White RA,Papadopoulos FC,Edvinsson Å,Kamali-Moghaddam M,Cunningham JL,Sundström-Poromaa I,Skalkidou A

    更新日期:2020-07-01 00:00:00

  • GDNF-related factor persephin is widely distributed throughout the nervous system.

    abstract::Persephin (PSP) is the most recently discovered member of the GDNF family of neurotrophic factors. We have used an RT-PCR approach to start addressing the putative functional significance of PSP by determining sites of its synthesis in the neonatal rat brain. Generally, two transcripts were found. Sequence analysis of...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4547(19980815)53:4<494::AID-JNR

    authors: Jaszai J,Farkas L,Galter D,Reuss B,Strelau J,Unsicker K,Krieglstein K

    更新日期:1998-08-15 00:00:00

  • Plasticity of hippocampus following perinatal asphyxia: effects on postnatal apoptosis and neurogenesis.

    abstract::Asphyxia during delivery produces long-term deficits in brain development, including hippocampus. We investigated hippocampal plasticity after perinatal asphyxia, measuring postnatal apoptosis and neurogenesis. Asphyxia was performed by immersing rat fetuses with uterine horns removed from ready-to-deliver rats into a...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.21715

    authors: Morales P,Fiedler JL,Andrés S,Berrios C,Huaiquín P,Bustamante D,Cardenas S,Parra E,Herrera-Marschitz M

    更新日期:2008-09-01 00:00:00

  • Diffusible factor(s) from adult rat sciatic nerve increases cell number and neurite outgrowth of cultured embryonic ventral mesencephalic tyrosine hydroxylase-positive neurons.

    abstract::Dissociated embryonic rat ventral mesencephalon containing the developing A8-A10 dopamine (DA) neurons was cultured alone or in the presence of a 10 mm segment of adult rat sciatic nerve that had been explanted and maintained in separate culture for 72 hours prior to introduction to mesencephalic cultures. Nerve segme...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490270318

    authors: Collier TJ,Sladek CD,Gallagher MJ,Gereau RW 4th,Springer JE

    更新日期:1990-11-01 00:00:00

  • Effect of cyclic AMP on the expression of myelin basic protein species and myelin proteolipid protein in committed oligodendrocytes: differential involvement of the transcription factor CREB.

    abstract::Our previous results support the idea that CREB (cyclic AMP-response element binding protein) may be a mediator of neuroligand and growth factor signals that, coupled to different signal transduction pathways, play different roles at specific stages of oligodendrocyte development. In the early stages, when cells are i...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.1195

    authors: Afshari FS,Chu AK,Sato-Bigbee C

    更新日期:2001-10-01 00:00:00

  • Possible role of scavenger receptor SRCL in the clearance of amyloid-beta in Alzheimer's disease.

    abstract::Accumulation of beta-amyloid protein (Abeta) in the brain is a hallmark of Alzheimer's disease (AD), and Abeta-mediated pathogenesis could result from increased production of Abeta or insufficient Abeta clearance by microglia, astrocytes, or the vascular system. Cell-surface receptors, such as scavenger receptors, mig...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.20992

    authors: Nakamura K,Ohya W,Funakoshi H,Sakaguchi G,Kato A,Takeda M,Kudo T,Nakamura T

    更新日期:2006-09-01 00:00:00

  • A difference in the in vivo cerebral production of [1-14C] lactate from D-[3-14C] glucose in chronic mental patients.

    abstract::Data from a study in which 12 chronic mental patients and 12 control subject were given D-[3-14C] glucose intravenously in our arterio-venous technique for determining cerebral metabolism in vivo were reexamined. Previously unpublished whole-blood lactate determinations in these experiments indicated a cerebral produc...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490060209

    authors: Sacks W,Schechter DC,Sacks S

    更新日期:1981-01-01 00:00:00

  • Platelet-derived growth factor and basic fibroblast growth factor regulate cell proliferation and the expression of notch-1 receptor in a new oligodendrocyte cell line.

    abstract::We generated a new cell line, N38, by conditionally immortalizing mouse oligodendrocytes (OLs) at early stages of maturation. The morphology and marker expression pattern suggest N38 cells are similar to immature OLs. N38 cells were sensitive to changes in serum concentrations, and forcing the cells to differentiate i...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/1097-4547(20001101)62:3<319::AID-JNR1>3.0.

    authors: Bongarzone ER,Byravan S,Givogri MI,Schonmann V,Campagnoni AT

    更新日期:2000-11-01 00:00:00

  • Expression of insulin-like growth factor-binding protein messenger RNAs in developing rat oligodendrocytes and astrocytes.

    abstract::Insulin-like growth factors, IGF-I and IGF-II, are potent regulators of oligodendrocyte development. Most of the IGF present in vivo is bound to members of a family of six high-affinity IGF-binding proteins (IGFBPs), which can either potentiate or inhibit IGF action, depending on other conditions. Additionally, serum ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4547(19971201)50:5<721::AID-JNR

    authors: Mewar R,McMorris FA

    更新日期:1997-12-01 00:00:00

  • Nerve growth factor-mediated increases in protein methylation occur predominantly at type I arginine methylation sites and involve protein arginine methyltransferase 1.

    abstract::Nerve growth factor (NGF)-specific signal transduction leads to changes in protein methylation during neuronal differentiation of PC12 cells (Cimato et al. [1997] J. Cell Biol. 138:1089-1103). In the present work, we demonstrate that, among NGF-regulated proteins, arginine methylation is more prevalent than carboxylme...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.10123

    authors: Cimato TR,Tang J,Xu Y,Guarnaccia C,Herschman HR,Pongor S,Aletta JM

    更新日期:2002-02-15 00:00:00

  • Expression of myelin protein gene transcripts by Schwann cells of regenerating nerve.

    abstract::The expression of many myelin-specific molecules in Schwann cells is profoundly decreased following denervation. This study examines the early reexpression of myelin protein genes associated with reinnervation. Following sciatic nerve crush, the distal, regenerated nerve was divided into appropriate (2.5 or 5 mm) cons...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490270202

    authors: Mitchell LS,Griffiths IR,Morrison S,Barrie JA,Kirkham D,McPhilemy K

    更新日期:1990-10-01 00:00:00

  • Overexpression of Akt (protein kinase B) confers protection against apoptosis and prevents formation of ceramide in response to pro-apoptotic stimuli.

    abstract::An immortalized dorsal root ganglion cell line F-11 exhibits many properties of spinal cord neurons and undergoes apoptosis in response to growth factor withdrawal and the exogenous addition of inhibitors of phosphatidylinositol-3-kinase (PI3K). To elucidate the mechanism of apoptosis we generated F-11 clones which ov...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:

    authors: Goswami R,Kilkus J,Dawson SA,Dawson G

    更新日期:1999-09-15 00:00:00

  • Contribution of amygdala pathology to comorbid emotional disturbances in temporal lobe epilepsy.

    abstract::The amygdala contributes to the generation and propagation of epileptiform activity in temporal lobe epilepsy (TLE). Ictal symptoms such as fear, dreamy states (déjà vu, memory flashbacks, experiential hallucinations), epigastric auras, or sympathetic outflow with cardiovascular changes are often linked to a seizure f...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审

    doi:10.1002/jnr.23689

    authors: Yilmazer-Hanke D,O'Loughlin E,McDermott K

    更新日期:2016-06-01 00:00:00

  • Plasma melatonin, pinealocyte morphology, and surface receptors/antigen expression on macrophages/microglia in the pineal gland following a high-altitude exposure.

    abstract::The present study examined the effects of high-altitude exposure on the pineal gland, the main source of production of melatonin. It was surmised that hypoxia experienced at high altitude, caused by decreased oxygen tension in the ambient air, might lead to some structural alterations in the pineal gland and, hence, a...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.10136

    authors: Kaur C,Srinivasan KN,Singh J,Peng CM,Ling EA

    更新日期:2002-02-15 00:00:00

  • Cyclin-dependent kinase 5 (cdk5) activation requires interaction with three domains of p35.

    abstract::Cyclin-dependent kinase 5 (cdk5), in contrast to other members of the cyclin-dependent kinase family, is not activated by cyclins but instead is activated by complexing with neuron-specific activator molecules (p35, p39, and p67). The most effective activator of cdk5 both in vitro and in vivo is p35. We have taken a k...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.10116

    authors: Amin ND,Albers W,Pant HC

    更新日期:2002-02-01 00:00:00

  • Sex differences modulating serotonergic polymorphisms implicated in the mechanistic pathways of risk for depression and related disorders.

    abstract::Despite consistent observations of sex differences in depression and related emotional disorders, we do not yet know how these sex differences modulate the effects of genetic polymorphisms implicated in risk for these disorders. This Mini-Review focuses on genetic polymorphisms of the serotonergic system to illustrate...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审

    doi:10.1002/jnr.23877

    authors: Perry LM,Goldstein-Piekarski AN,Williams LM

    更新日期:2017-01-02 00:00:00

  • CADASIL: extended polymorphisms and mutational analysis of the NOTCH3 gene.

    abstract::CADASIL is a cerebrovascular disease caused by mutations in the NOTCH3 gene. Most mutations result in a gain or loss of cysteine residue in one of the 34 epidermal growth factor-like repeats in the extracellular domain of the Notch3 protein, thus sparing the number of cysteine residues. To date, more than 130 differen...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.21935

    authors: Ungaro C,Mazzei R,Conforti FL,Sprovieri T,Servillo P,Liguori M,Citrigno L,Gabriele AL,Magariello A,Patitucci A,Muglia M,Quattrone A

    更新日期:2009-04-01 00:00:00

  • Corelease of two functionally opposite neurotransmitters by retinal amacrine cells: experimental evidence and functional significance.

    abstract::The Dale's law postulates that a neuron releases the same neurotransmitter from all its branches. In the case of multiple neurotransmitters it would require all transmitters to be released from all branches. The retinal cholinergic amacrine cells contain and release gamma-aminobutyric (GABA) and, therefore, if GABA an...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审

    doi:10.1002/(sici)1097-4547(19991115)58:4<475::aid-jnr

    authors: Duarte CB,Santos PF,Carvalho AP

    更新日期:1999-11-15 00:00:00

  • Roles of metabotropic glutamate receptors in glial function and glial-neuronal communication.

    abstract::The amino acid glutamate plays a key role in brain function. One of the major roles of glutamate is to mediate fast excitatory neurotransmission via activation of ionotropic glutamate receptors (iGluRs). More recently, however, it has become clear that glutamate also serves a regulatory function through activation of ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审

    doi:10.1002/(SICI)1097-4547(19961015)46:2<131::AID-JNR

    authors: Winder DG,Conn PJ

    更新日期:1996-10-15 00:00:00

  • Mitogen activated protein (MAP) kinase activity is increased in adult mouse superior cervical ganglia during culturing.

    abstract::We have used adult mouse superior cervical ganglia (SCG) to study the role of mitogen activated protein (MAP) kinase activity during axonal outgrowth in vitro. An initial peak in activity within the first hours of culture was followed by a substantially higher activity after 1 to 2 days, a time when axons were activel...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/(SICI)1097-4547(19980515)52:4<453::AID-JNR

    authors: Svensson B,Ekström PA

    更新日期:1998-05-15 00:00:00

  • Granulocyte colony-stimulating factor improves neuron survival in experimental spinal cord injury by regulating nucleophosmin-1 expression.

    abstract::Granulocyte colony-stimulating factor (G-CSF) and its related mechanisms were investigated to assess the potential for this factor to exert neuroprotective effects against spinal cord injury in mice. Recombinant human granulocyte colony-stimulating factor (rhG-CSF) was injected into mice spinal cord hemisection models...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.23362

    authors: Guo Y,Liu S,Wang P,Zhang H,Wang F,Bing L,Gao J,Yang J,Hao A

    更新日期:2014-06-01 00:00:00

  • Cholesterol reutilization during myelination of regenerating PNS axons is impaired in Niemann-Pick disease type C mice.

    abstract::Niemann-Pick C (NPC) disease is an autosomal recessive lipidosis characterized by lysosomal accumulations of unesterified cholesterol. Cultured NPC cells exhibit a defect in the intracellular trafficking of LDL-derived cholesterol that leads to lysosomal accumulations of unesterified cholesterol. We found in a prelimi...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/(sici)1097-4547(19970801)49:3<389::aid-jnr

    authors: Goodrum JF,Pentchev PG

    更新日期:1997-08-01 00:00:00

  • Synergistic neuroprotective effects of combined treatment with olmesartan plus azelnidipine in stroke-prone spontaneously hypertensive rats.

    abstract::An angiotensin 2 type 1 receptor blocker, olmesartan, and a calcium channel blocker, azelnidipine, possess not only an antihypertensive effect but also an antioxidative effect and other beneficial effects. In the present study, we examined the efficacy of olmesartan and azelnidipine monotherapy (2 mg/kg or 10 mg/kg ea...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.23406

    authors: Omote Y,Deguchi K,Kono S,Liu W,Kurata T,Hishikawa N,Yamashita T,Ikeda Y,Abe K

    更新日期:2014-10-01 00:00:00

  • Altered genetic response to beta-adrenergic receptor activation in late passage C6 glioma cells.

    abstract::Previous studies have demonstrated variability in the phenotype of rat C6 glioma cells. In the present study, we compared morphology, growth rate, and beta-adrenergic regulation of gene expression in early (P39-47) and late (P55-90) passage C6 cells. Morphological changes were observed in five independently derived, l...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.490330213

    authors: Gubits RM,Yu H,Casey G,Munell F,Vitek MP

    更新日期:1992-10-01 00:00:00

  • Long-term effects of JL 13, a potential atypical antipsychotic, on rat dopamine and serotonin receptor subtypes.

    abstract::Changes in dopamine (DA) D(1), D(2), D(3), and D(4) receptors and serotonin 5-HT(1A) and 5-HT(2A) receptors in rat forebrain regions were autoradiographically quantified after continuous infusion of JL 13 [(5-(4-methylpiperazin-1-yl)-8-chloro-pyrido[2,3-b][1,5]benzoxazepine fumarate] for 28 days with osmotic minipumps...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.20972

    authors: Moran-Gates T,Massari C,Graulich A,Liégeois JF,Tarazi FI

    更新日期:2006-08-15 00:00:00

  • GAD67 and GAD65 mRNA and protein expression in cerebrocortical regions of elderly patients with schizophrenia.

    abstract::Gamma-Aminobutyric acid (GABA), the principal inhibitory neurotransmitter of CNS, has been consistently implicated in the pathophysiology of schizophrenia. GABA is synthesized from glutamate by the enzyme glutamic acid decarboxylase (GAD). Two isoforms of GAD have been identified and have been named GAD65 and GAD67 ba...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.20122

    authors: Dracheva S,Elhakem SL,McGurk SR,Davis KL,Haroutunian V

    更新日期:2004-05-15 00:00:00

  • mRNAs encoding the Aplysia homologues of fasciclin-I and beta-thymosin are expressed only in the second phase of nerve injury and are differentially segregated in axons regenerating in vitro and in vivo.

    abstract::Studies using Aplysia californica have demonstrated that transcription after nerve injury occurs during a rapid, transient first phase and a delayed, prolonged second phase. Although the second phase is especially important for regeneration, the mRNAs produced during this phase have not been identified. We characteriz...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.20645

    authors: Colby GP,Sung YJ,Ambron RT

    更新日期:2005-11-15 00:00:00

  • mRNA expression of ephrins and Eph receptor tyrosine kinases in the neonatal and adult mouse central nervous system.

    abstract::Ephrins and Eph receptors are a family of molecules that have been implicated in axonal pathfinding. A unique feature of B-class ephrins and Eph receptors is their ability to transmit bidirectional signals in both ephrin- and Eph receptor-expressing cells upon cell-cell contact. These signals can lead to cytoskeletal ...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章

    doi:10.1002/jnr.10457

    authors: Liebl DJ,Morris CJ,Henkemeyer M,Parada LF

    更新日期:2003-01-01 00:00:00

  • Neurons rely on glucose rather than astrocytic lactate during stimulation.

    abstract::Brain metabolism increases during stimulation, but this increase does not affect all energy metabolism equally. Briefly after stimulation, there is a local increase in cerebral blood flow and in glucose uptake, but a smaller increase in oxygen uptake. This indicates that temporarily the rate of glycolysis is faster th...

    journal_title:Journal of neuroscience research

    pub_type: 杂志文章,评审

    doi:10.1002/jnr.24374

    authors: Díaz-García CM,Yellen G

    更新日期:2019-08-01 00:00:00