Abstract:
:The role of the transcription factor AP-1 in regulating D2 receptor transcriptional activity was investigated in D2 receptor expressing neuroblastoma cells, NB41A3, and in non-D2 receptor expressing CHO cells. Deletion of a region containing the putative AP-1 binding site resulted in a significant reduction in the activity in CHO cells; while the activity in NB41A3 cells was increased suggesting that the AP-1 site may differentially regulate D2 gene expression in these distinct cell types. However, both cell lines were found to express significant and similar levels of the transcription factors AP-1. Analysis of phosphorylated proteins in each of the cell lines provided evidence that AP-1 is phosphorylated in NB41A3 cells, but not in CHO cells. This result suggests that differential regulation of D2 gene expression may be related to AP-1 phosphorylation.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Wang J,Miller JC,Friedhoff AJdoi
10.1002/(SICI)1097-4547(19971001)50:1<23::AID-JNR3subject
Has Abstractpub_date
1997-10-01 00:00:00pages
23-31issue
1eissn
0360-4012issn
1097-4547pii
10.1002/(SICI)1097-4547(19971001)50:1<23::AID-JNR3journal_volume
50pub_type
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