Abstract：
:Recent studies have demonstrated that neural stem cell (NSC) culture at physiologically normoxic conditions (2-5% O2) is advantageous in terms of neuronal differentiation and survival. Neuronal differentiation is accompanied by a remarkable shift to mitochondrial oxidative metabolism compared with preferentially glycolytic metabolism of proliferating cells. However, metabolic changes induced by growth in a normoxic (5%) O2 culture environment in NSCs have been minimally explored. This study demonstrates that culturing under 5% O2 conditions results in higher levels of mitochondrial oxidative metabolism, decreased glycolysis, and reduced levels of reactive oxygen species in NSC cultures. Inflammation is one of the major environmental factors limiting postinjury NSC neuronal differentiation and survival. Our results show that NSCs differentiated under 5% O2 conditions possess better resistance to in vitro inflammatory injury compared with those exposed to 20% O2. The present work demonstrates that lower, more physiologically normal O2 levels support metabolic changes induced during NSC neuronal differentiation and provide increased resistance to inflammatory injury, thus highlighting O2 tension as an important determinant of cell fate and survival in various stem cell therapies.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Sun X,Voloboueva LA,Stary CM,Giffard RGdoi
10.1002/jnr.23615subject
Has Abstractpub_date
2015-11-01 00:00:00pages
1703-12issue
11eissn
0360-4012issn
1097-4547journal_volume
93pub_type
杂志文章abstract：:Human T-cell lymphotropic virus type I (HTLV-I) may infect up to 10% of peripheral blood T cells in patients with HTLV-I myelopathy. To examine the impact of HTLV-I infection on the abilities of T cells to present and respond to peptide antigen, we HTLV-I infected and subcloned a myelin basic protein peptide 84-102 (M...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19960915)45:6<786::AID-JNR
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abstract：:Cultures of chicken day 8 embryo retinal cells, essentially free of contaminating non-neuronal elements, were used to examine the neurotoxicity of various excitatory amino acid transmitter receptor agonists. At 7 days in vitro, N-methyl-D-aspartate (NMDA), following 24 hr exposure to 0.1-1.0 mM, destroyed 60-70% of th...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
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abstract：:Brain-derived neurotrophic factor (BDNF) plays an important role in the differentiation, development, and survival of neural stem cells. In this study, we analyzed its effects on the stimulation of human umbilical cord blood-derived mesenchymal stem cells in terms of their potential to differentiate into neuron-like c...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21669
更新日期：2008-08-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10667
更新日期：2003-08-15 00:00:00
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journal_title：Journal of neuroscience research
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doi：10.1002/jnr.20666
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journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19981115)54:4<495::AID-JNR
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abstract：:The normal innervation of structures in mouse foot pads was investigated with immunohistochemistry and confocal microscopy. Nerves were visualized by incubating Zamboni fixed, thick, frozen sections with antibodies to protein gene product 9.5 (PGP 9.5), vasoactive intestinal peptide, substance P, calcitonin gene-relat...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490410113
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22119
更新日期：2009-10-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490350209
更新日期：1993-06-01 00:00:00
abstract：:The distribution of both tyrosine hydroxylase (TH) antigen and messenger RNA was investigated in the ventral mesencephalon from human brain using a 35S-labeled human TH cDNA probe and a polyclonal TH antibody. A hybridization signal was detected not only in the perikaryon but also in the neurites, most probably dendri...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490250415
更新日期：1990-04-01 00:00:00
abstract：:The galactolipids galactocerebroside and sulfatide have been implicated in oligodendrocyte (OL) development and myelin formation. Much of the early evidence for myelin galactolipid function has been derived from antibody and chemical perturbation of OLs in vitro. To determine the role of these lipids in vivo, we previ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22003
更新日期：2009-11-15 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20440
更新日期：2005-04-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23503
更新日期：2015-02-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490240308
更新日期：1989-11-01 00:00:00
abstract：:Heat shock proteins (HSPs) are stress-responsive proteins that serve as important molecules contributing to cellular "protein triage." We and others have reported an increase of selected HSPs in multiple sclerosis (MS) lesions. However, the exact expression pattern of HSP family genes in MS is not known. The aim of ou...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23079
更新日期：2012-10-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19980415)52:2<201::AID-JNR
更新日期：1998-04-15 00:00:00
abstract：:Transforming growth factor-β1 (TGF-β1) is an important member of multifunctional growth factor superfamily. It has been implicated in pain signaling, but little is known about the underlying mechanisms. Herein, we report that TGF-β1 can exert a sustained enhancing effect on the functional activity of acid-sensing ion ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.24481
更新日期：2019-10-01 00:00:00
abstract：:The effects of glutamate on intracellular free Ca2+, [Ca2+]i, and neurotoxicity were compared in cerebellar granule neurons in vitro. [Ca2+]i was measured with fura-2 and digital fluorescence imaging microscopy; neurotoxicity was monitored using a vital dye and colorimetric analysis. Glutamate produced dose-dependent ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490280317
更新日期：1991-03-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
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更新日期：2012-01-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
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journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
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更新日期：2020-07-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.1182
更新日期：2001-09-15 00:00:00
abstract：:The Shaw subfamily of potassium channel genes, including Kv3.1, are highly expressed within the auditory nuclei of the brainstem, where they have been implicated in the characteristic response properties of particular types of neurons. Potassium currents carried by Kv3.1 are voltage-dependent, have a high activation t...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(sici)1097-4547(19991215)58:6<791::aid-jnr
更新日期：1999-12-15 00:00:00
abstract：:Microarray analyses demonstrated that a variety of genes was affected by treatment of cerebellar granule cell neurons with the neurotrophic factor pigment epithelium-derived factor (PEDF). The genes for neurotrophins, glial cell-derived neurotrophic factor (GDNF), and their receptors were regulated differentially in i...
journal_title：Journal of neuroscience research
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doi：10.1002/jnr.20196
更新日期：2004-09-01 00:00:00
abstract：:Using a canine model of classical late-infantile neuronal ceroid lipofuscinosis (CLN2 disease), a study was conducted to evaluate the potential pharmacological activity of recombinant human tripeptidyl peptidase-1 (rhTPP1) enzyme replacement therapy administered directly to the cerebrospinal fluid (CSF). CLN2 disease ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23423
更新日期：2014-11-01 00:00:00
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journal_title：Journal of neuroscience research
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更新日期：2017-10-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
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更新日期：1999-11-15 00:00:00
abstract：:Iron deficiency and chronic mild carbon monoxide (CO) exposure are nutritional and environmental problems that can be experienced simultaneously. We examined the effects of chronic mild CO exposure and iron availability on auditory development in the rat. We propose that chronic mild CO exposure creates an oxidative s...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20495
更新日期：2005-06-01 00:00:00
abstract：:The activity levels of pyruvate dehydrogenase, enzymes of the citric acid cycle, aspartate and alanine aminotransferases, and NADP+-isocitrate dehydrogenase were determined in the cerebral cortex, cerebellum, brain stem, corpus striatum, hippocampus, and midbrain regions of normal rats and rats injected with acute and...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490140407
更新日期：1985-01-01 00:00:00
