Abstract：
:Differentiated human NT2-N neurons were shown to express CCR5 and CXCR4 chemokine receptor mRNA and protein, and to be responsive to the chemokines CCL5 and CXCL12. Using cDNA microarray technology, CCL5 was found to induce a distinct transcriptional program, with reproducible induction of 46 and 9 genes after 2 and 8 hr of treatment, respectively. Conversely, downregulation of 20 and 7 genes was observed after 2 and 8 hr of treatment, respectively. Modulation of a selected panel of CCL5-responsive genes was also confirmed by quantitative RT-PCR and Western blot and compared to gene expression changes induced by CXCL12 treatment. Gene clustering identified distinct functional subsets of CCL5-responsive molecules, and a significant number of expressed sequence tags encoding unknown genes. CCL5-responsive genes comprise a significant number of enzymes, transcription factors, and miscellaneous molecules involved in neuronal survival and differentiation, including neurite outgrowth and synaptogenesis. Our results suggest that CCL5 biological functions might go beyond its recognized chemotactic activity in the central nervous system, in particular with regard to the control of neural plasticity events both during development and in postnatal life.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Valerio A,Ferrario M,Martinez FO,Locati M,Ghisi V,Bresciani LG,Mantovani A,Spano Pdoi
10.1002/jnr.20250keywords:
subject
Has Abstractpub_date
2004-11-01 00:00:00pages
371-82issue
3eissn
0360-4012issn
1097-4547journal_volume
78pub_type
杂志文章abstract：:We have performed proteomic analysis in the cerebrospinal fluid in an animal model of Parkinson's disease induced by axotomy of the medial forebrain bundle. In this model, the degeneration of dopaminergic neurons was completed in 14 days, with a loss of about 50% dopaminergic neurons in the substantia nigra and a loss...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.21452
更新日期：2007-12-01 00:00:00
abstract：:Double immunofluorescence studies using antibodies against NF-L and peripherin revealed three distinct subpopulations of neurons in rat dorsal root ganglia (DRG). In the adult rat, 46% of the DRG neurons were small and peripherin-positive (NF-L-negative), and 48% were large and NF-L-positive (peripherin-negative). Abo...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490300111
更新日期：1991-09-01 00:00:00
abstract：:We have previously shown in rat primary cultured mesencephalic cells that cyanide induces a high level of oxidative stress and necrotic death. To evaluate the mechanism of the cytotoxicity, the effects of cyanide on intracellular glutathione (GSH) pools and inducible nitric oxide synthase (iNOS)-mediated reactive nitr...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20998
更新日期：2006-10-01 00:00:00
abstract：:The brainstem nucleus of the solitary tract (NTS) is the key integrating relay in the central processing of sensory information from the thoracic and from most subdiaphragmatic viscera. Modulation of neuronal excitability and synaptic activity in the NTS by nicotinic agents can have potent effects on vital physiologic...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.23436
更新日期：2014-11-01 00:00:00
abstract：:Tungstate treatment increases the phosphorylation of glycogen synthase kinase-3beta (GSK3beta) at serine 9, which triggers its inactivation both in cultured neural cells and in vivo. GSK3 phosphorylation is dependent on the activation of extracellular signal-regulated kinases 1/2 (ERK1/2) induced by tungstate. As a co...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20726
更新日期：2006-02-01 00:00:00
abstract：:The presence of glycosaminoglycans (GAG) has been histochemically demonstrated in the CNS of various mammalian species. They have been related with some nerve functions as neurotransmitters storage and synaptic transmission. In the present paper, the histochemical properties of nerve cell cytoplasmic GAG were studied ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490110103
更新日期：1984-01-01 00:00:00
abstract：:Fractionation of muscle microsomes rich in sarcoplasmic reticulum (SR) by isopicnic centrifugation yielded three types of membranes. Heavy (HM), intermediate (IM), and light membranes (LM), with isopicnic points of 38, 33, and 25% w/w sucrose, were rich in terminal cisternae/triads, longitudinal SR, and T-tubules, res...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490330409
更新日期：1992-12-01 00:00:00
abstract：:The engrailed gene has been identified in Drosophila as an important developmental gene involved in the control of segmentation. Here we describe the embryonic expression of a chicken gene, ChickEn (Darnell et al.: J Cell Biol 103(5):311a, 1986), which contains homology to the Drosophila engrailed gene. Northern blots...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490210234
更新日期：1988-10-01 00:00:00
abstract：:The twitcher mouse is well known as a naturally occurring authentic mouse model of human globoid cell leukodystrophy (GLD; Krabbe disease) due to genetic deficiency of lysosomal galactosylceramidase. The twitcher mice used most commonly are on the C57BL/6J background. We generated twitcher mice that were on the mixed ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20190
更新日期：2004-08-15 00:00:00
abstract：:To study the sequence of degenerative events possibly associated with cholinergic cell death in Alzheimer's disease, septal cholinergic neurons derived from rat embryonic brains were exposed to chronic excitotoxic stress by glutamate. Counts of choline acetyltransferase (ChAT)-immunopositive neurons and measurement of...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490400608
更新日期：1995-04-15 00:00:00
abstract：:There is supporting evidence of alcohol negative effects on the brain: neuroimaging and psychophysiological studies finding anatomical and functional connectivity (FC) changes associated with the dependence process. Thus, the aim of this work was to evaluate brain FC and network characteristics of alcohol-dependent in...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.24673
更新日期：2020-10-01 00:00:00
abstract：:Cytoskeletal structures under the cell membrane carry out pivotal roles in the maintenance and remodeling of the cell structures. Reforming of the cytoskeletal networks after partial extraction of membrane components could be a good clue to identify molecular components pertaining the interaction of cytoskeleton with ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20555
更新日期：2005-07-15 00:00:00
abstract：:Parkinson's disease (PD) is the most common movement disorder associated with the elderly which, other than symptomatic therapies, has no effective treatment or preventive measures. Sirtuins and their pharmacological activators/inhibitors have been associated with a range of neuroprotective effects, and a large body o...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.23806
更新日期：2017-04-01 00:00:00
abstract：:Cultures of chicken day 8 embryo retinal cells, essentially free of contaminating non-neuronal elements, were used to examine the neurotoxicity of various excitatory amino acid transmitter receptor agonists. At 7 days in vitro, N-methyl-D-aspartate (NMDA), following 24 hr exposure to 0.1-1.0 mM, destroyed 60-70% of th...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490270210
更新日期：1990-10-01 00:00:00
abstract：:A mouse monoclonal antibody (5B9), directed against a carbohydrate epitope of human epidermal growth factor receptor (EGFR), recognized an 81-kDalton glycoprotein in buffer-soluble and detergent-solubilized rat brain extracts (BE). The glycoprotein was more abundant in extracts prepared from injured brain than in thos...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490400609
更新日期：1995-04-15 00:00:00
abstract：:Adrenal chromaffin cell (ACC) transplants, alone or combined with levodopa treatment, were used in attempted therapy for Parkinson's disease (PD). In a previous study, we demonstrated that levodopa caused chromaffin cell death either by necrosis or by apoptosis in cell culture. Here we report the beneficial effect of ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10456
更新日期：2003-01-01 00:00:00
abstract：:The extension of filopodia from growth cones of regenerating neurites from rat superior cervical ganglion neurons in tissue culture was studied. Cultures were grown on a thin layer of fibrous collagen and maintained in a medium containing serum and nerve growth factor. Time-lapse cinematography and computer-assisted m...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490130111
更新日期：1985-01-01 00:00:00
abstract：:Acoustic overstimulation causes apoptotic cell death in the cochlea. This death process is mediated, in part, by the mitochondrial signaling pathway involving Bcl-2 family proteins. Myeloid cell leukemia sequence 1 (Mcl-l) is an antiapoptotic member of the Bcl-2 family. Its involvement in noise-induced hair cell death...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22333
更新日期：2010-06-01 00:00:00
abstract：:An immortalized dorsal root ganglion cell line F-11 exhibits many properties of spinal cord neurons and undergoes apoptosis in response to growth factor withdrawal and the exogenous addition of inhibitors of phosphatidylinositol-3-kinase (PI3K). To elucidate the mechanism of apoptosis we generated F-11 clones which ov...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：
更新日期：1999-09-15 00:00:00
abstract：:Raf kinases are downstream effectors of Ras and upstream activators of the MEK-ERK cascade. Ras and MEK-ERK signaling play roles in learning and memory (L&M) and neural plasticity, but the roles of Raf kinases in L&M and plasticity are unclear. Among Raf isoforms, B-raf is preferentially expressed in the brain. To det...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20703
更新日期：2006-01-01 00:00:00
abstract：:The response of cells localized in the brain subventricular zone (SVZ) to growth factor stimulation has been largely described for development and adult life, whereas no information on their behavior during aging is available. To address the question of whether the cells in the SVZ of old mice respond to the intracere...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10614
更新日期：2003-06-01 00:00:00
abstract：:The galactolipids galactocerebroside and sulfatide have been implicated in oligodendrocyte (OL) development and myelin formation. Much of the early evidence for myelin galactolipid function has been derived from antibody and chemical perturbation of OLs in vitro. To determine the role of these lipids in vivo, we previ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22003
更新日期：2009-11-15 00:00:00
abstract：:The p53 tumor suppressor gene, which is considered the guardian of the genome, encodes a phosphoprotein, which is a sequence-specific transcriptional activator or repressor of target genes. The role of p53 in developmental processes has not been studied extensively, although its expression appears to undergo temporal ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19990515)56:4<427::AID-JNR
更新日期：1999-05-15 00:00:00
abstract：:The midbrain central gray (periaqueductal gray; PAG) mediates defensive behaviors and is implicated in the rewarding effects of opiate drugs. Projections from the PAG to the ventral tegmental area (VTA) suggest that this region might also regulate behaviors involving motivation and cognition. However, studies have not...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22265
更新日期：2010-04-01 00:00:00
abstract：:In the present study we report self-assembly of individual neurofilament (NF) triplet proteins (70K, 150K, and 200K daltons) isolated by anion exchange chromatography from bovine spinal cord. Formation of smooth 10-nm filaments by both NF 150K and NF 70K is shown. Optimal conditions for NK 150K filament formation were...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490110204
更新日期：1984-01-01 00:00:00
abstract：:The blood-brain barrier (BBB) refers to the network of microvessels that selectively restricts the passage of substances between the circulation and the central nervous system (CNS). This microvascular network is comprised of arterioles, capillaries and venules, yet the respective contribution of each of these to the ...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22316
更新日期：2010-05-15 00:00:00
abstract：:Soluble amyloid beta protein (Abeta) oligomers have been considered recently to be responsible for the cognitive dysfunction that sets in prior to senile plaque formation in the Alzheimer's disease (AD) brain. By using the newly prepared antibody against oligomer Abeta, rather than fibrillar or monomer Abeta, we obser...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20952
更新日期：2006-08-15 00:00:00
abstract：:The amygdala contributes to the generation and propagation of epileptiform activity in temporal lobe epilepsy (TLE). Ictal symptoms such as fear, dreamy states (déjà vu, memory flashbacks, experiential hallucinations), epigastric auras, or sympathetic outflow with cardiovascular changes are often linked to a seizure f...
journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：10.1002/jnr.23689
更新日期：2016-06-01 00:00:00
abstract：:Here we show that rabbit Müller cell differentiation from radial glial progenitor cells is accompanied by a decreasing capability to respond to specific stimuli (depolarization and extracellular adenosine 5'-triphosphate [ATP]) with an elevation of intracellular calcium. Intracellular free calcium was recorded in reti...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.10406
更新日期：2002-10-15 00:00:00
abstract：:Toll-like receptors (TLRs) play an essential role in initiating intracellular type I interferon (IFN)-mediated innate immunity against viral infections. We examined whether human neuronal cells (primary human neurons, NT2-N and CHP-212 cells) express TLRs and mount type I IFN-mediated innate immunity against herpes si...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22110
更新日期：2009-10-01 00:00:00
