Abstract:
:A rapid and sensitive assay for pyridinium oximes in plasma and tissue was developed. The method was suitable for the analysis of mono- and di-pyridinium oximes and utilizes ultrafiltration followed by cation-exchange high-performance liquid chromatography with UV detection. The assay was originally developed for the measurement of the oxime MMB-4 in plasma for which the lower limit of detection was 0.0005 pg and the limit of quantitation was 0.001 to 2.5 microg. The assay required as little as 50 microL of whole blood or 30 pL of tissue homogenate, and it was used for a pharmacokinetic study from a single intramuscular injection of MMB-4 (dichloride or dimethylsulfonate salt) in the guinea pig. Both salts were found to have similar pharmacokinetic properties in the plasma with a T1/2 of about 34 to 42 min and the area-under-the-curve values increased dose dependently. MMB-4 tissue concentrations were much lower than the plasma. The tissue levels peaked at 5-20 min depending on the tissue. A rank of concentration was diaphragm > heart > thigh muscle.
journal_name
J Anal Toxicoljournal_title
Journal of analytical toxicologyauthors
Singh H,Moorad-Doctor D,Ratcliffe RH,Wachtel K,Castillo A,Garcia GEdoi
10.1093/jat/31.2.69subject
Has Abstractpub_date
2007-03-01 00:00:00pages
69-74issue
2eissn
0146-4760issn
1945-2403journal_volume
31pub_type
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