当前位置： SCI文献检索 > GUT期刊下所有文献 > Intraluminal acid induces oesophageal shortening via capsaicin-sensitive neurokinin neurons.

Intraluminal acid induces oesophageal shortening via capsaicin-sensitive neurokinin neurons.

Abstract：

OBJECTIVE:Intraluminal acid evokes reflex contraction of oesophageal longitudinal smooth muscle (LSM) and consequent oesophageal shortening. This reflex may play a role in the pathophysiology of oesophageal pain syndromes and hiatus hernia formation. The aim of the current study was to elucidate further the mechanisms of acid-induced oesophageal shortening. DESIGN:Intraluminal acid perfusion of the intact opossum smooth muscle oesophagus was performed in vitro in the presence and absence of neural blockade and pharmacological antagonism of the neurokinin 2 receptor, while continuously recording changes in oesophageal axial length. In addition, the effect of these antagonists on the contractile response of LSM strips to the mast cell degranulating agent 48/80 was determined. Finally, immunohistochemistry was performed to look for evidence of LSM innervation by substance P/calcitonin gene-related peptide (CGRP)-containing axons. RESULTS:Intraluminal acid perfusion induced longitudinal axis shortening that was completely abolished by capsaicin desensitization, substance P desensitization, or the application of the neurokinin 2 receptor antagonist MEN10376. Compound 48/80 induced sustained contraction of LSM strips in a concentration-dependent fashion and this was associated with evidence of mast cell degranulation. The 48/80-induced LSM contraction was antagonized by capsaicin desensitization, substance P desensitization and MEN10376, but not tetrodotoxin. Immunohistochemistry revealed numerous substance P/CGRP-containing neurons innervating the LSM and within the mucosa. CONCLUSIONS:This study suggests that luminal acid activates a reflex pathway involving mast cell degranulation, activation of capsaicin-sensitive afferent neurons and the release of substance P or a related neurokinin, which evokes sustained contraction of the oesophageal LSM. This pathway may be a target for treatment of oesophageal pain syndromes.

journal_name

Gut

journal_title

Gut

authors

Paterson WG,Miller DV,Dilworth N,Assini JB,Lourenssen S,Blennerhassett MG

doi

10.1136/gut.2006.115881

subject

Has Abstract

pub_date

2007-10-01 00:00:00

pages

1347-52

issue

eissn

0017-5749

issn

1468-3288

pii

gut.2006.115881

journal_volume

pub_type

杂志文章

在线工具

Intraluminal acid induces oesophageal shortening via capsaicin-sensitive neurokinin neurons.