Abstract：
:The nonstructural protein 3 (NS3) of members of the family Flaviviridae possesses multiple enzyme activities that are likely to be essential for viral replication. Here, we cloned and expressed full-length CSFV NS3 protein (NS3FL) and its N-terminal truncated version (ntNS3) in E. coli. NTPase activities of the purified NS3FL and ntNS3 proteins and their reaction conditions were investigated. The results showed that CSFV NS3FL and ntNS3 proteins contained a specific polynucleotide-stimulated NTPase acitivity. Characterization of ntNS3 NTPase activity showed that optimal reaction conditions with respect to pH, MgCl2 and monovalent cations were similar to those of bovine viral diarrhea virus (BVDV) and hepatitis C virus (HCV). Site-directed mutagenesis analysis demonstrated that the GxGK(232)T to GxGAT mutation in the conserved motif I abolished the NTPase activity of ntNS3, whereas substitution of TATPA(354) for TATPV in the motif III had no effect on the enzyme activity. Moreover, the kinetic properties (K(m) and k(cat)) of CSFV NS3 were more similar to those of BVDV. Our results provide insight into the structure-function relationship of CSFV NS3 and facilitate our understanding of its role in the replication cycle of CSFV.
journal_name
Arch Viroljournal_title
Archives of virologyauthors
Wen G,Chen C,Luo X,Wang Y,Zhang C,Pan Zdoi
10.1007/s00705-007-0969-2subject
Has Abstractpub_date
2007-01-01 00:00:00pages
1565-73issue
8eissn
0304-8608issn
1432-8798journal_volume
152pub_type
杂志文章abstract：:Peritoneal exudate cells (PEC) of DBA/2 mice, after 7 days of in vitro preculture and consisting of virtually 100 per cent macrophages, were able to support the replication of Herpes Simplex Virus type 1 strain WAL (HSV). Using a standard medium based on Dulbecco's Modified Eagle Medium (D-MEM), no virus replication w...
journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309370
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journal_title：Archives of virology
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doi：10.1007/s00705-006-0851-7
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journal_title：Archives of virology
pub_type： 杂志文章
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journal_title：Archives of virology
pub_type： 杂志文章
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doi：10.1007/s00705-015-2583-z
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pub_type： 杂志文章
doi：10.1007/BF01309297
更新日期：1984-01-01 00:00:00
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doi：10.1007/s00705-012-1562-x
更新日期：2013-04-01 00:00:00
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更新日期：2008-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/BF01309785
更新日期：1993-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/BF01309695
更新日期：1992-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s007050050332
更新日期：1998-01-01 00:00:00
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journal_title：Archives of virology
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更新日期：2014-10-01 00:00:00
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pub_type： 杂志文章
doi：10.1007/s007050050630
更新日期：1999-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
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更新日期：2002-01-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-017-3634-4
更新日期：2018-03-01 00:00:00
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journal_title：Archives of virology
pub_type： 杂志文章
doi：10.1007/s00705-015-2562-4
更新日期：2015-11-01 00:00:00
