Abstract:
:Regulation of ion transport by phosphorylation and G proteins occurs in several epithelial and non-epithelial cell plasma membranes1-5. It is not known whether transporters on intracellular membranes are target sites for second messengers. Here we present direct evidence that a chloride conductance in endocytic vesicles from rabbit proximal tubule is activated by phosphorylation through a cyclic AMP-dependent protein kinase. To measure chloride transport, endocytic vesicles were labelled in vivo with a Cl(-)-sensitive fluorescent indicator6-8. It was found that labelled endosomes contained an inward proton pump and a chloride conductance, but no ion-coupled chloride transport, and that the chloride conductance was regulated by protein kinase A. These results, taken together with measurements of chloride effects on ATP-dependent acidification, suggest that endosomal pH can be controlled by phosphorylation of a stilbene-sensitive conductive chloride transporter.
journal_name
Naturejournal_title
Natureauthors
Bae HR,Verkman ASdoi
10.1038/348637a0subject
Has Abstractpub_date
1990-12-13 00:00:00pages
637-9issue
6302eissn
0028-0836issn
1476-4687journal_volume
348pub_type
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