Abstract:
:During platelet activation there is a major reorganization in the platelet cytoskeleton that accompanies a rapid change in platelet shape. Many of the events associated with activation are attributed to a rise in calcium concentration within the platelet cytoplasm. One direct consequence of the elevated calcium is the activation of a calcium-dependent protease that cleaves a major platelet protein of relative molecular mass (Mr) approximately 235,000 (235K) to 200K. This protein, P235, has been purified and reported to interact with actin, but the significance of the proteolytic cleavage is unknown. Talin, a cytoskeletal protein in smooth muscle and fibroblasts, binds vinculin and, together with vinculin, is localized in fibroblasts at sites of actin-membrane attachment. Talin and P235 have similar purification procedures, sedimentation coefficients and Stokes' radii (ref. 6 and Molony et al., unpublished observations). Of particular significance, talin is readily cleaved by proteases from approximately 215K to a fragment of approximately 190K. Given these similarities we have investigated the possible relationship between these proteins. Here we demonstrate that platelet P235 is recognized by anti-talin antibody and that it binds vinculin. Both proteins are cleaved in vitro by the calcium-activated protease to yield similar fragments. We conclude that P235 corresponds to the platelet form of talin.
journal_name
Naturejournal_title
Natureauthors
O'Halloran T,Beckerle MC,Burridge Kdoi
10.1038/317449a0subject
Has Abstractpub_date
1985-10-03 00:00:00pages
449-51issue
6036eissn
0028-0836issn
1476-4687journal_volume
317pub_type
杂志文章相关文献
NATURE文献大全abstract::The mouse genetic revolution has shown repeatedly that most organs have more functions than expected. This has led to the realization that, in addition to a molecular and cellular approach, there is a need for a whole-organism study of physiology. The skeleton is an example of how a whole-organism approach to physiolo...
journal_title:Nature
pub_type: 杂志文章,评审
doi:10.1038/nature10763
更新日期:2012-01-18 00:00:00
abstract::Understanding the behaviour of the dielectric constant in ferroelectric thin films remains a challenging problem. These ferroelectric materials have high static dielectric constants, and so are important for their applications in high-storage-density capacitor structures such as dynamic random access memory (DRAM). Bu...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35006023
更新日期:2000-03-23 00:00:00
abstract::The discovery of the peptide hormone ghrelin, an endogenous ligand for the growth hormone secretagogue (GHS) receptor, yielded the surprising result that the principal site of ghrelin synthesis is the stomach and not the hypothalamus. Although ghrelin is likely to regulate pituitary growth hormone (GH) secretion along...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35038090
更新日期:2000-10-19 00:00:00
abstract::The four-chambered mammalian heart develops from two fields of cardiac progenitor cells distinguished by their spatiotemporal patterns of differentiation and contributions to the definitive heart. The first heart field differentiates earlier in lateral plate mesoderm, generates the linear heart tube and ultimately giv...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature10094
更新日期:2011-05-29 00:00:00
abstract::The haemagglutinin (HA) glycoproteins of influenza virus membranes are responsible for binding viruses to cells by interacting with membrane receptor molecules which contain sialic acid (for review see ref. 1). This interaction is known to vary in detailed specificity for different influenza viruses (see, for example,...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/304076a0
更新日期:1983-07-07 00:00:00
abstract::Ground- and aircraft-based measurements show that the seasonal amplitude of Northern Hemisphere atmospheric carbon dioxide (CO2) concentrations has increased by as much as 50 per cent over the past 50 years. This increase has been linked to changes in temperate, boreal and arctic ecosystem properties and processes suc...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature13957
更新日期:2014-11-20 00:00:00
abstract::The modern giant sperm whale Physeter macrocephalus, one of the largest known predators, preys upon cephalopods at great depths. Lacking a functional upper dentition, it relies on suction for catching its prey; in contrast, several smaller Miocene sperm whales (Physeteroidea) have been interpreted as raptorial (versus...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature09067
更新日期:2010-07-01 00:00:00
abstract::Apoptosis is a highly regulated process that is crucial for normal development and homeostasis of multicellular organisms. The p35 protein from baculoviruses effectively prevents apoptosis by its broad-spectrum caspase inhibition. Here we report the crystal structure of p35 in complex with human caspase-8 at 3.0 A res...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35068604
更新日期:2001-03-22 00:00:00
abstract::Fullerenes are graphitic cage structures incorporating exactly twelve pentagons. The smallest possible fullerene is thus C20, which consists solely of pentagons. But the extreme curvature and reactivity of this structure have led to doubts about its existence and stability. Although theoretical calculations have ident...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/35024037
更新日期:2000-09-07 00:00:00
abstract::Scientific theories of how subduction and plate tectonics began on Earth--and what the tectonic structure of Earth was before this--remain enigmatic and contentious. Understanding viable scenarios for the onset of subduction and plate tectonics is hampered by the fact that subduction initiation processes must have bee...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature15752
更新日期:2015-11-12 00:00:00
abstract::The distribution, accumulation and circulation of oxygen and hydrogen in Earth's interior dictate the geochemical evolution of the hydrosphere, atmosphere and biosphere. The oxygen-rich atmosphere and iron-rich core represent two end-members of the oxygen-iron (O-Fe) system, overlapping with the entire pressure-temper...
journal_title:Nature
pub_type: 历史文章,杂志文章
doi:10.1038/nature18018
更新日期:2016-06-09 00:00:00
abstract::The unusual helium-rich (type Ib) supernova SN 2005E is distinguished from all supernovae hitherto observed by its faint and rapidly fading light curve, prominent calcium lines in late-phase spectra and lack of any mark of recent star formation near the supernova location. These properties are claimed to be explained ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature09055
更新日期:2010-05-20 00:00:00
abstract::The accumulation of somatic DNA damage has been implicated as a cause of ageing in metazoa. One possible mechanism by which increased DNA damage could lead to cellular degeneration and death is by stochastic deregulation of gene expression. Here we directly test for increased transcriptional noise in aged tissue by di...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature04844
更新日期:2006-06-22 00:00:00
abstract::In general, heterozygous animal cells express both alleles at a particular locus. The only exceptions are cells of XX genotype after inactivation of one X chromosome, and immunoglobulin-producing cells; in each case only one of the two alleles is expressed in differentiated cells and their progeny. This phenomenon, te...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/284179a0
更新日期:1980-03-13 00:00:00
abstract::The extinction rate of populations is predicted to rise under increasing rates of environmental change. If a population experiencing increasingly stressful conditions lacks appropriate phenotypic plasticity or access to more suitable habitats, then genetic change may be the only way to avoid extinction. Evolutionary r...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature11879
更新日期:2013-02-28 00:00:00
abstract::Recent studies have shown that developing nerve fibres grow directly to their targets and are guided by specific cues, but the nature of these cues and the mechanism of guidance remain unknown. The growth of sympathetic axons towards an artificial source of nerve growth factor (NGF) in vivo and of sensory neurites up ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/306786a0
更新日期:1983-12-22 00:00:00
abstract::Mechanotransduction has an important role in physiology. Biological processes including sensing touch and sound waves require as-yet-unidentified cation channels that detect pressure. Mouse Piezo1 (MmPiezo1) and MmPiezo2 (also called Fam38a and Fam38b, respectively) induce mechanically activated cationic currents in c...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature10812
更新日期:2012-02-19 00:00:00
abstract::Leukaemia inhibitory factor (LIF) has a variety of effects on different cell types in vitro, inhibiting the differentiation of embryonic stem cells and promoting the survival and/or proliferation of primitive haematopoietic precursors and primordial germ cells. Here we show that LIF-deficient mice derived by gene targ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/363361a0
更新日期:1993-05-27 00:00:00
abstract::An amino acid sequence analysis of the N-terminal immunoglobulin heavy and light chain variable regions (VH and VL) from 16 hybridoma proteins which bind phosphorylcholine as well as the complete sequence analysis of 9 of these VH regions is presented. There seem to be more VH regions participating in the phosphorylch...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/291029a0
更新日期:1981-05-07 00:00:00
abstract::Interfaces in heterostructures have been a key point of interest in condensed-matter physics for decades owing to a plethora of distinctive phenomena-such as rectification1, the photovoltaic effect2, the quantum Hall effect3 and high-temperature superconductivity4-and their critical roles in present-day technical devi...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/s41586-020-2602-4
更新日期:2020-08-01 00:00:00
abstract::Tectal polarity for retinal connections remains reversible long after the anatomical pattern of neural structures has been determined. Cells in the diencephalon seem to control this polarity. Following certain embryonic operations, the diencephalon developed behind the tectum. In such cases, the polarity of the retino...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/258126a0
更新日期:1975-11-13 00:00:00
abstract::Substrates of the proteasome are recognized and unfolded by the regulatory particle, and then translocated into the core particle (CP) to be degraded. A hetero-hexameric ATPase ring, containing subunits Rpt1-6, is situated within the base subassembly of the regulatory particle. The ATPase ring sits atop the CP, with t...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature08065
更新日期:2009-06-11 00:00:00
abstract::Insulin resistance occurs in a variety of conditions, including diabetes, obesity and essential hypertension, but its underlying molecular mechanisms are unclear. In type 2 (non-insulin-dependent) diabetes mellitus, it is insulin-resistance in skeletal muscle, the chief site of insulin-mediated glucose disposal in hum...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/335632a0
更新日期:1988-10-13 00:00:00
abstract::Acquired uniparental disomy (aUPD) is a common feature of cancer genomes, leading to loss of heterozygosity. aUPD is associated not only with loss-of-function mutations of tumour suppressor genes, but also with gain-of-function mutations of proto-oncogenes. Here we show unique gain-of-function mutations of the C-CBL (...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature08240
更新日期:2009-08-13 00:00:00
abstract::Long-term potentiation (LTP) and long-term depression (LTD) are activity-dependent changes in synaptic strength that may serve as the cellular mechanisms of information storage in the vertebrate brain. The mossy fibre-CA3 synapse displays NMDA (M-methyl-D-aspartate) receptor-independent forms of LTP and LTD that were ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/381429a0
更新日期:1996-05-30 00:00:00
abstract::In response to adenosine 5'-diphosphate, the P2Y1 receptor (P2Y1R) facilitates platelet aggregation, and thus serves as an important antithrombotic drug target. Here we report the crystal structures of the human P2Y1R in complex with a nucleotide antagonist MRS2500 at 2.7 Å resolution, and with a non-nucleotide antago...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature14287
更新日期:2015-04-16 00:00:00
abstract::The inner ear contains sensory epithelia that detect head movements, gravity and sound. It is unclear how to develop these sensory epithelia from pluripotent stem cells, a process that will be critical for modelling inner ear disorders or developing cell-based therapies for profound hearing loss and balance disorders....
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature12298
更新日期:2013-08-08 00:00:00
abstract::Fluids and melts liberated from subducting oceanic crust recycle lithophile elements back into the mantle wedge, facilitate melting and ultimately lead to prolific subduction-zone arc volcanism. The nature and composition of the mobile phases generated in the subducting slab at high pressures have, however, remained l...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/nature03971
更新日期:2005-09-29 00:00:00
abstract::Plasmodium falciparum infected erythrocytes containing mature trophozoites and schizonts sequester along venular endothelium and are not in the peripheral circulation of patients with malaria. Knobs appear on infected erythrocytes and are the points of attachment to endothelium. Sequestration may protect the parasite ...
journal_title:Nature
pub_type: 杂志文章
doi:10.1038/318064a0
更新日期:1985-11-07 00:00:00
abstract::The recent decades-long decline in East African rainfall suggests that multidecadal variability is an important component of the climate of this vulnerable region. Prior work based on analysing the instrumental record implicates both Indian and Pacific ocean sea surface temperatures (SSTs) as possible drivers of East ...
journal_title:Nature
pub_type: 历史文章,杂志文章
doi:10.1038/nature11785
更新日期:2013-01-17 00:00:00