eIF5B gates the transition from translation initiation to elongation.

Abstract:

:Translation initiation determines both the quantity and identity of the protein that is encoded in an mRNA by establishing the reading frame for protein synthesis. In eukaryotic cells, numerous translation initiation factors prepare ribosomes for polypeptide synthesis; however, the underlying dynamics of this process remain unclear1,2. A central question is how eukaryotic ribosomes transition from translation initiation to elongation. Here we use in vitro single-molecule fluorescence microscopy approaches in a purified yeast Saccharomyces cerevisiae translation system to monitor directly, in real time, the pathways of late translation initiation and the transition to elongation. This transition was slower in our eukaryotic system than that reported for Escherichia coli3-5. The slow entry to elongation was defined by a long residence time of eukaryotic initiation factor 5B (eIF5B) on the 80S ribosome after the joining of individual ribosomal subunits-a process that is catalysed by this universally conserved initiation factor. Inhibition of the GTPase activity of eIF5B after the joining of ribosomal subunits prevented the dissociation of eIF5B from the 80S complex, thereby preventing elongation. Our findings illustrate how the dissociation of eIF5B serves as a kinetic checkpoint for the transition from initiation to elongation, and how its release may be governed by a change in the conformation of the ribosome complex that triggers GTP hydrolysis.

journal_name

Nature

journal_title

Nature

authors

Wang J,Johnson AG,Lapointe CP,Choi J,Prabhakar A,Chen DH,Petrov AN,Puglisi JD

doi

10.1038/s41586-019-1561-0

subject

Has Abstract

pub_date

2019-09-01 00:00:00

pages

605-608

issue

7775

eissn

0028-0836

issn

1476-4687

pii

10.1038/s41586-019-1561-0

journal_volume

573

pub_type

杂志文章

相关文献

NATURE文献大全
  • Language-tree divergence times support the Anatolian theory of Indo-European origin.

    abstract::Languages, like genes, provide vital clues about human history. The origin of the Indo-European language family is "the most intensively studied, yet still most recalcitrant, problem of historical linguistics". Numerous genetic studies of Indo-European origins have also produced inconclusive results. Here we analyse l...

    journal_title:Nature

    pub_type: 历史文章,杂志文章

    doi:10.1038/nature02029

    authors: Gray RD,Atkinson QD

    更新日期:2003-11-27 00:00:00

  • Bmi-1 determines the proliferative capacity of normal and leukaemic stem cells.

    abstract::An emerging concept in the field of cancer biology is that a rare population of 'tumour stem cells' exists among the heterogeneous group of cells that constitute a tumour. This concept, best described with human leukaemia, indicates that stem cell function (whether normal or neoplastic) might be defined by a common se...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature01572

    authors: Lessard J,Sauvageau G

    更新日期:2003-05-15 00:00:00

  • Real-life stories of online harassment - and how scientists got through it.

    abstract:: ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/d41586-018-07046-0

    authors: Gewin V

    更新日期:2018-10-01 00:00:00

  • Dicer recognizes the 5' end of RNA for efficient and accurate processing.

    abstract::A hallmark of RNA silencing is a class of approximately 22-nucleotide RNAs that are processed from double-stranded RNA precursors by Dicer. Accurate processing by Dicer is crucial for the functionality of microRNAs (miRNAs). The current model posits that Dicer selects cleavage sites by measuring a set distance from th...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature10198

    authors: Park JE,Heo I,Tian Y,Simanshu DK,Chang H,Jee D,Patel DJ,Kim VN

    更新日期:2011-07-13 00:00:00

  • Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme.

    abstract::One goal of regenerative medicine, to use stem cells to replace cells lost by injury or disease, depends on producing an excess of the relevant cell for study or transplantation. To this end, the stepwise differentiation of stem cells into specialized derivatives has been successful for some cell types, but a major pr...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature11463

    authors: Sneddon JB,Borowiak M,Melton DA

    更新日期:2012-11-29 00:00:00

  • Structure of the human P2Y12 receptor in complex with an antithrombotic drug.

    abstract::P2Y receptors (P2YRs), a family of purinergic G-protein-coupled receptors (GPCRs), are activated by extracellular nucleotides. There are a total of eight distinct functional P2YRs expressed in human, which are subdivided into P2Y1-like receptors and P2Y12-like receptors. Their ligands are generally charged molecules w...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature13083

    authors: Zhang K,Zhang J,Gao ZG,Zhang D,Zhu L,Han GW,Moss SM,Paoletta S,Kiselev E,Lu W,Fenalti G,Zhang W,Müller CE,Yang H,Jiang H,Cherezov V,Katritch V,Jacobson KA,Stevens RC,Wu B,Zhao Q

    更新日期:2014-05-01 00:00:00

  • Linguistic perception: neural processing of a whistled language.

    abstract::Silbo Gomero is a whistled language that is a rare and endangered surrogate of Spanish, used by shepherds on the island of La Gomera in the Canary Islands for communication over long distances on difficult terrain. Here we show that areas of the brain normally associated with spoken-language function are also activate...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/433031a

    authors: Carreiras M,Lopez J,Rivero F,Corina D

    更新日期:2005-01-06 00:00:00

  • Ancestral echinoderms from the Chengjiang deposits of China.

    abstract::Deuterostomes are a remarkably diverse super-phylum, including not only the chordates (to which we belong) but groups as disparate as the echinoderms and the hemichordates. The phylogeny of deuterostomes is now achieving some degree of stability, especially on account of new molecular data, but this leaves as conjectu...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature02648

    authors: Shu DG,Morris SC,Han J,Zhang ZF,Liu JN

    更新日期:2004-07-22 00:00:00

  • Transposon tagging of a male sterility gene in Arabidopsis.

    abstract::Transformation of the well-studied maize transposable elements into other plant species should enable transposon tagging methodology to be used for the isolation of interesting genes in the heterologous host. Here we describe the isolation of a transposon-tagged male sterile mutant in Arabidopsis thaliana using the ma...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/363715a0

    authors: Aarts MG,Dirkse WG,Stiekema WJ,Pereira A

    更新日期:1993-06-24 00:00:00

  • Observation of nuclear fusion driven by a pyroelectric crystal.

    abstract::While progress in fusion research continues with magnetic and inertial confinement, alternative approaches--such as Coulomb explosions of deuterium clusters and ultrafast laser-plasma interactions--also provide insight into basic processes and technological applications. However, attempts to produce fusion in a room t...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature03575

    authors: Naranjo B,Gimzewski JK,Putterman S

    更新日期:2005-04-28 00:00:00

  • Biodiversity loss and its impact on humanity.

    abstract::The most unique feature of Earth is the existence of life, and the most extraordinary feature of life is its diversity. Approximately 9 million types of plants, animals, protists and fungi inhabit the Earth. So, too, do 7 billion people. Two decades ago, at the first Earth Summit, the vast majority of the world's nati...

    journal_title:Nature

    pub_type: 杂志文章,评审

    doi:10.1038/nature11148

    authors: Cardinale BJ,Duffy JE,Gonzalez A,Hooper DU,Perrings C,Venail P,Narwani A,Mace GM,Tilman D,Wardle DA,Kinzig AP,Daily GC,Loreau M,Grace JB,Larigauderie A,Srivastava DS,Naeem S

    更新日期:2012-06-06 00:00:00

  • Allograft theory: transmission of devil facial-tumour disease.

    abstract::The Tasmanian devil, a large carnivorous Australian marsupial, is under threat from a widespread fatal disease in which a malignant oral-facial tumour obstructs the animal's ability to feed. Here we show that the chromosomes in these tumours have undergone a complex rearrangement that is identical for every animal stu...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/439549a

    authors: Pearse AM,Swift K

    更新日期:2006-02-02 00:00:00

  • Associative, bidirectional modifications at the hippocampal mossy fibre-CA3 synapse.

    abstract::Long-term potentiation (LTP) and long-term depression (LTD) are activity-dependent changes in synaptic strength that may serve as the cellular mechanisms of information storage in the vertebrate brain. The mossy fibre-CA3 synapse displays NMDA (M-methyl-D-aspartate) receptor-independent forms of LTP and LTD that were ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/381429a0

    authors: Derrick BE,Martinez JL Jr

    更新日期:1996-05-30 00:00:00

  • Nucleation of microtubule assembly by a gamma-tubulin-containing ring complex.

    abstract::The highly conserved protein gamma-tubulin is required for microtubule nucleation in vivo. When viewed in the electron microscope, a highly purified gamma-tubulin complex from Xenopus consisting of at least seven different proteins is seen to have an open ring structure. This complex acts as an active microtubule-nucl...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/378578a0

    authors: Zheng Y,Wong ML,Alberts B,Mitchison T

    更新日期:1995-12-07 00:00:00

  • The observable signature of late heating of the Universe during cosmic reionization.

    abstract::Models and simulations of the epoch of reionization predict that spectra of the 21-centimetre transition of atomic hydrogen will show a clear fluctuation peak, at a redshift and scale, respectively, that mark the central stage of reionization and the characteristic size of ionized bubbles. This is based on the assumpt...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature12999

    authors: Fialkov A,Barkana R,Visbal E

    更新日期:2014-02-13 00:00:00

  • Controlling anisotropic nanoparticle growth through plasmon excitation.

    abstract::Inorganic nanoparticles exhibit size-dependent properties that are of interest for applications ranging from biosensing and catalysis to optics and data storage. They are readily available in a wide variety of discrete compositions and sizes. Shape-selective synthesis strategies now also yield shapes other than nanosp...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature02020

    authors: Jin R,Cao YC,Hao E,Métraux GS,Schatz GC,Mirkin CA

    更新日期:2003-10-02 00:00:00

  • Thalamic control of sensory selection in divided attention.

    abstract::How the brain selects appropriate sensory inputs and suppresses distractors is unknown. Given the well-established role of the prefrontal cortex (PFC) in executive function, its interactions with sensory cortical areas during attention have been hypothesized to control sensory selection. To test this idea and, more ge...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature15398

    authors: Wimmer RD,Schmitt LI,Davidson TJ,Nakajima M,Deisseroth K,Halassa MM

    更新日期:2015-10-29 00:00:00

  • Multiple modes of engrailed regulation in the progression towards cell fate determination.

    abstract::The engrailed gene product of Drosophila specifies the fate of a subset of cells in each segment. Our studies of engrailed regulation suggest that fate determination is an elaborate, multistep process. At the time in embryogenesis when the engrailed-dependent cell fate is probably determined, four modes of control act...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/352404a0

    authors: Heemskerk J,DiNardo S,Kostriken R,O'Farrell PH

    更新日期:1991-08-01 00:00:00

  • RSPO2 inhibition of RNF43 and ZNRF3 governs limb development independently of LGR4/5/6.

    abstract::The four R-spondin secreted ligands (RSPO1-RSPO4) act via their cognate LGR4, LGR5 and LGR6 receptors to amplify WNT signalling1-3. Here we report an allelic series of recessive RSPO2 mutations in humans that cause tetra-amelia syndrome, which is characterized by lung aplasia and a total absence of the four limbs. Fun...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/s41586-018-0118-y

    authors: Szenker-Ravi E,Altunoglu U,Leushacke M,Bosso-Lefèvre C,Khatoo M,Thi Tran H,Naert T,Noelanders R,Hajamohideen A,Beneteau C,de Sousa SB,Karaman B,Latypova X,Başaran S,Yücel EB,Tan TT,Vlaminck L,Nayak SS,Shukla A,Giris

    更新日期:2018-05-01 00:00:00

  • Single neurons in prefrontal cortex encode abstract rules.

    abstract::The ability to abstract principles or rules from direct experience allows behaviour to extend beyond specific circumstances to general situations. For example, we learn the 'rules' for restaurant dining from specific experiences and can then apply them in new restaurants. The use of such rules is thought to depend on ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/35082081

    authors: Wallis JD,Anderson KC,Miller EK

    更新日期:2001-06-21 00:00:00

  • Embryonic and extraembryonic stem cell lines derived from single mouse blastomeres.

    abstract::The most basic objection to human embryonic stem (ES) cell research is rooted in the fact that ES cell derivation deprives embryos of any further potential to develop into a complete human being. ES cell lines are conventionally isolated from the inner cell mass of blastocysts and, in a few instances, from cleavage st...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature04277

    authors: Chung Y,Klimanskaya I,Becker S,Marh J,Lu SJ,Johnson J,Meisner L,Lanza R

    更新日期:2006-01-12 00:00:00

  • Assembly of the inner rod determines needle length in the type III secretion injectisome.

    abstract::Assembly of multi-component supramolecular machines is fundamental to biology, yet in most cases, assembly pathways and their control are poorly understood. An example is the type III secretion machine, which mediates the transfer of bacterial virulence proteins into host cells. A central component of this nanomachine...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature04822

    authors: Marlovits TC,Kubori T,Lara-Tejero M,Thomas D,Unger VM,Galán JE

    更新日期:2006-06-01 00:00:00

  • Genome-wide analysis identifies NR4A1 as a key mediator of T cell dysfunction.

    abstract::T cells become dysfunctional when they encounter self antigens or are exposed to chronic infection or to the tumour microenvironment1. The function of T cells is tightly regulated by a combinational co-stimulatory signal, and dominance of negative co-stimulation results in T cell dysfunction2. However, the molecular m...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/s41586-019-0979-8

    authors: Liu X,Wang Y,Lu H,Li J,Yan X,Xiao M,Hao J,Alekseev A,Khong H,Chen T,Huang R,Wu J,Zhao Q,Wu Q,Xu S,Wang X,Jin W,Yu S,Wang Y,Wei L,Wang A,Zhong B,Ni L,Liu X,Nurieva R,Ye L,Tian Q,Bian XW,Dong C

    更新日期:2019-03-01 00:00:00

  • Visualization of ordered genomic RNA and localization of transcriptional complexes in rotavirus.

    abstract::In double-stranded-RNA (dsRNA) viruses found in animals, bacteria and yeast, the genome is transcribed within the structurally intact core of the virion with extraordinary efficiency. The structural organization of the genome and the enzymes involved in the transcription inside any of these viruses, critical for under...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/382471a0

    authors: Prasad BV,Rothnagel R,Zeng CQ,Jakana J,Lawton JA,Chiu W,Estes MK

    更新日期:1996-08-01 00:00:00

  • Structure of guanine-nucleotide-exchange factor human Mss4 and identification of its Rab-interacting surface.

    abstract::Guanine-nucleotide-exchange factors (GEFs) promote the exchange of GDP for GTP in Ras GTPases, and thereby positively regulate their functions. Members of the Sec4/Ypt1/Rab branch of the Ras superfamily are essential for vesicular transport. A GEF for a subset of Rab proteins, termed mammalian suppressor of Sec4 (Mss4...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/376788a0

    authors: Yu H,Schreiber SL

    更新日期:1995-08-31 00:00:00

  • Activation and disruption of a neural mechanism for novel choice in monkeys.

    abstract::Neural mechanisms that mediate the ability to make value-guided decisions have received substantial attention in humans and animals1-6. Experiments in animals typically involve long training periods. By contrast, choices in the real world often need to be made between new options spontaneously. It is therefore possibl...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/s41586-020-03115-5

    authors: Bongioanni A,Folloni D,Verhagen L,Sallet J,Klein-Flügge MC,Rushworth MFS

    更新日期:2021-01-06 00:00:00

  • Competition for antigen presentation in living cells involves exchange of peptides bound by class II MHC molecules.

    abstract::T cells recognize foreign proteins as peptides bound to self molecules encoded by the major histocompatibility complex (MHC). The kinetics of interaction between purified class II MHC molecules and peptides is unusual, in that the rate of association is very slow, but once formed, the complexes are extremely stable. T...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/342800a0

    authors: Adorini L,Appella E,Doria G,Cardinaux F,Nagy ZA

    更新日期:1989-12-14 00:00:00

  • Ocular dominance shift in kitten visual cortex caused by imbalance in retinal electrical activity.

    abstract::Monocular lid suture during the sensitive period early in the life of a kitten disrupts normal development of inputs from the two eyes to the visual cortex, causing a decrease in the fraction of cortical cells responding to the deprived eye. Such an ocular dominance shift has been assumed to depend on patterned visual...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/324154a0

    authors: Chapman B,Jacobson MD,Reiter HO,Stryker MP

    更新日期:1986-11-13 00:00:00

  • The SMAD2/3 interactome reveals that TGFβ controls m6A mRNA methylation in pluripotency.

    abstract::The TGFβ pathway has essential roles in embryonic development, organ homeostasis, tissue repair and disease. These diverse effects are mediated through the intracellular effectors SMAD2 and SMAD3 (hereafter SMAD2/3), whose canonical function is to control the activity of target genes by interacting with transcriptiona...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature25784

    authors: Bertero A,Brown S,Madrigal P,Osnato A,Ortmann D,Yiangou L,Kadiwala J,Hubner NC,de Los Mozos IR,Sadée C,Lenaerts AS,Nakanoh S,Grandy R,Farnell E,Ule J,Stunnenberg HG,Mendjan S,Vallier L

    更新日期:2018-03-08 00:00:00

  • Crystal structure of nucleotide-free dynamin.

    abstract::Dynamin is a mechanochemical GTPase that oligomerizes around the neck of clathrin-coated pits and catalyses vesicle scission in a GTP-hydrolysis-dependent manner. The molecular details of oligomerization and the mechanism of the mechanochemical coupling are currently unknown. Here we present the crystal structure of h...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature10369

    authors: Faelber K,Posor Y,Gao S,Held M,Roske Y,Schulze D,Haucke V,Noé F,Daumke O

    更新日期:2011-09-18 00:00:00