Abstract:
:An antigen-specific T-T hybridoma was mutagenized with ethylmethane sulfonate and negatively selected by anti-Ly-6 antibody-induced growth inhibition. One of the mutants generated, M4/8, had lost surface expression of a T cell receptor (TcR) V beta 8 epitope detected on the surface of the parental cell line. However, the mutant cell line did express high levels of TcR heterodimer as detected with a pan-specific anti-TcR antibody. CD3 epsilon, Ly-6 and Thy-1 were expressed at levels similar to the wild-type parental cell line. Analysis of the surface TcR/CD3 complexes by immunoprecipitation and two-dimension gel electrophoresis confirmed that the major discernable difference between the wild-type and mutant TcR/CD3 complexes resided in the TcR beta chain. The parental cell line had the potential to express two TcR heterodimers, V alpha V beta 1 and V alpha V beta 8, as determined by Northern blot analysis. Co-modulation experiments suggested that both types of receptors were expressed. However, the V alpha V beta 8 receptor was the predominant form. In contrast, the mutant M4/8 cell line did not synthesize V beta 8 mRNA and, thus, only the V alpha V beta 1 TcR was synthesized. Despite the normal surface expression of TcR/CD3 complex, the M4/8 mutant cell line did not produce interleukin 2 (IL 2) in response to antigen or soluble anti-CD3 epsilon monoclonal antibody (mAb). Furthermore, it responded poorly to concanavalin A, phytohemagglutinin and anti-Ly-6 mAb. Cross-linking of the stimulatory antibodies partially restored the IL 2 response to anti-CD3 epsilon or anti-Ly-6 to wild-type levels. Phorbol ester and ionomycin stimulated a full IL 2 response in the M4/8 cell line, demonstrating that the defect in the decreased signaling in the mutant did not result from a defect in the IL 2 gene program. In conclusion, these data suggested that the pairing of alpha/beta heterodimer not only determined antigen/MHC specificity but also the signaling efficiency of the TcR/CD3 complex.
journal_name
Eur J Immunoljournal_title
European journal of immunologyauthors
Reno TA,Ley S,Sugiyama E,Cantagrel A,Blumberg R,Bonventre J,Terhorst C,Yeh ETdoi
10.1002/eji.1830200702subject
Has Abstractpub_date
1990-07-01 00:00:00pages
1417-22issue
7eissn
0014-2980issn
1521-4141journal_volume
20pub_type
杂志文章abstract::MS and EAE are T cell-driven autoimmune diseases of the CNS where IL-17-producing Th17 cells promote damage and are pathogenic. Conversely, tolerogenic DCs induce Treg cells and suppress Th17 cells. Chloroquine (CQ) suppresses EAE through the modulation of DCs by unknown mechanisms. Here, we show that STAT 1 is necess...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201747362
更新日期:2018-07-01 00:00:00
abstract::The specificity and efficiency of endogenous peptides in the HLA class I binding have been investigated by the use of a simple procedure that is based on the serological detection of the folding of HLA class I alpha chains that is induced by the binding to specific peptides in the presence of beta 2 microglobulin. HLA...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830220834
更新日期:1992-08-01 00:00:00
abstract::The autosomal-dominant hyper-IgE syndrome (HIES), caused by mutations in STAT3, is a rare primary immunodeficiency that predisposes to mucocutaneous candidiasis and staphylococcal skin and lung infections. This infection phenotype is suggestive of defects in neutrophils, but data on neutrophil functions in HIES are in...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201847650
更新日期:2018-12-01 00:00:00
abstract::We identified the presence in the Aleutian skate, Bathyraja aleutica, of two classes of immunoglobulins (Ig), a high molecular weight Ig analogous to mammalian IgM and a low molecular weight Ig, similarly to the spiny rasp skate, Raja kenojei, (Kobayashi, K. et al., Mol. Immunol. 1984. 21: 397), using an immunological...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830150916
更新日期:1985-09-01 00:00:00
abstract::The delivery of T-cell help to B cells is antigen-specific, MHC-restricted, and CD40L (CD154) dependent. It has been thought that when a T cell recognizes an antigen-presenting B cell, CD40L expressed on the T-cell surface engages with CD40 on the surface of B cells as long as the cells remain conjugated. By adding fl...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201646504
更新日期:2017-01-01 00:00:00
abstract::The proteins p40 (Mr = 40 000), p32 (Mr = 32 000), p28 (Mr = 28 000), p20 (Mr = 20 000) and p10 (Mr = 10 000) are described which occur in noncovalent association with the polymorphic alpha, beta heterodimer of class II antigens. They were investigated with respect to their molecular characteristics and their mutual s...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140606
更新日期:1984-06-01 00:00:00
abstract::Cyclosporin A (CsA), an immunosuppressive drug which completely suppresses the humoral response to thymus-dependent antigens, does not affect the generation of T helper (Th) cells during an in vitro stimulation of murine spleen cells with sheep red blood cells. The appearance of Th cells depends on time and their deve...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830210304
更新日期:1991-03-01 00:00:00
abstract::Chemokine receptor CXCR6 mediates the chemotaxis and adhesion of leukocytes to soluble and membrane-anchored forms of CXCL16, and is an HIV-1 co-receptor. Here, we describe the effects of mutation of acidic extracellular CXCR6 residues on receptor function. Although most CXCR6 mutants examined were expressed at levels...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200838269
更新日期:2008-08-01 00:00:00
abstract::The surface expression of given HLA class I alleles protects target cells from lysis mediated by natural killer (NK) clones specific for these (or related) alleles. We could define two groups of NK clones specifically recognizing either Cw4 and related C alleles ("group 1") or Cw3 and related C alleles ("group 2"), re...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240434
更新日期:1994-04-01 00:00:00
abstract::Heat-stable antigen (HSA)/mouse CD24 (formerly termed Nectadrin) is a membrane glycoprotein with an unusual structure consisting of a small protein core and extensive glycosylation. It is expressed by hematopoietic cells but not by mature T lymphocytes. HSA on accessory cells is an important costimulatory molecule req...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240336
更新日期:1994-03-01 00:00:00
abstract::Major histocompatibility complex (MHC) class II-associated antigen presentation is mainly linked to processing of exogenous antigens upon cellular uptake by endocytosis, but has also been observed for endogenously synthesized antigens. We have studied the MHC class II-associated presentation of the endogenously synthe...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830251230
更新日期:1995-12-01 00:00:00
abstract::Vaccination of mice with irradiated Schistosoma mansoni larvae confers high levels of immunity which is mediated by Th1-type lymphocytes. To investigate a possible role for IL-12 in the induction of protection, we have compared the immune response of IL-12 p40-deficient (KO) mice and their C57BL/6 (WT) counterparts fo...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199809)28:09<2827::AID-IMM
更新日期:1998-09-01 00:00:00
abstract::Toll-like receptors (TLRs) are playing important roles in stimulating the innate immune response and intensifying adaptive immune response against invading pathogens. Appropriate regulation of TLR activation is important to maintain a balance between preventing tumor activation and inhibiting autoimmunity. Toll-like r...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201545567
更新日期:2016-03-01 00:00:00
abstract::CD4+ alpha beta T cell populations that develop in mice deficient in MHC class II (through 'knockout' of either the Aalpha, or the Abeta chain of the I-A(b) molecule) comprise a major 'single-positive' (SP) CD4+ CD8- subset (60-90%) and a minor 'double-positive' (DP) CD4+ CD8alpha beta+ subset (10-40%). Many DP T cell...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200324463
更新日期:2004-03-01 00:00:00
abstract::Specific monoclonal antibodies (mAb) directed against the CD23 antigen were used to study human interleukin 4 (hIL4)-induced IgE production by blood and tonsillar mononuclear cells. Both peripheral blood and tonsillar mononuclear cells stimulated by hIL4 expressed membrane CD23 as detected by the binding of all anti-C...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830200120
更新日期:1990-01-01 00:00:00
abstract::A T3 complex-bearing subpopulation was characterized within an in vivo cycling T4-8- early thymocyte compartment which contains cells constitutively expressing interleukin 2 and transferrin receptors. We show differentiation in vitro of both mature subsets of thymocytes (T3+4+8- and T3+4-8+) from the above T4-8- compa...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830160611
更新日期:1986-06-01 00:00:00
abstract::The initiation of CD8(+) T cell (CTL) immune responses can occur via cross-priming. Recent data suggested a relationship between cross-presentation and immunodominance of epitope-specific T cells. To test this association, we evaluated the efficacy of cross-presentation for several virus epitopes in vitro and examined...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200939973
更新日期:2010-08-01 00:00:00
abstract::The analysis of an Ia specificity was performed in several H-2 haplotypes by evaluating the affinity of the binding to mouse spleen cells of a monoclonal anti-Ia alloantibody. We used the monoclonal antibody 10-2.16 (Oi et al., Curr. Top. Microbiol. Immunol. 1978. 81: 115.) which recognizes on I-A gene products a publ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830110912
更新日期:1981-09-01 00:00:00
abstract::Two major histocompatibility complex (MHC) class I-reactive T cell clones derived from H-2b mice, generated against the allogeneic Ld molecule, were found to recognize the H-2b class II mutant Abm12 molecule as well. In addition, these clones also recognize the class II A(s) molecule, and display a class II-dependent ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830230823
更新日期:1993-08-01 00:00:00
abstract::Interleukin-31 (IL-31) is a Th2 cell-derived cytokine that has been closely linked to pruritic skin inflammation. More recently, enhanced IL-31 serum levels have also been observed in patients with allergic rhinitis and allergic asthma. Therefore, the main aim of this study was to unravel the contribution of IL-31 to ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.202048547
更新日期:2020-07-10 00:00:00
abstract::Human invariant natural killer T (NKT) cell TCRs bind to CD1d via an "invariant" Vα24-Jα18 chain (iNKTα) paired to semi-invariant Vβ11 chains (iNKTβ). Single-amino acid variations at position 93 (p93) of iNKTα, immediately upstream of the "invariant" CDR3α region, have been reported in a substantial proportion of huma...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.201141956
更新日期:2012-01-01 00:00:00
abstract::A T cell activation antigen, Ly6C, is considered to be involved in the autoimmunity of some autoimmune-prone mice; however, the function of Ly6C remains largely unknown. We prepared a rat anti-mouse Ly6C monoclonal antibody (mAb) (S14) that inhibits the proliferation of peripheral T cells stimulated with anti-CD3 mAb ...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(SICI)1521-4141(199802)28:02<696::AID-IMMU
更新日期:1998-02-01 00:00:00
abstract::Adenosine triphosphate has previously been shown to induce semi-mature human monocyte-derived dendritic cells (DC). These are characterized by the up-regulation of co-stimulatory molecules, the inhibition of IL-12 and the up-regulation of some genes involved in immune tolerance, such as thrombospondin-1 and indoleamin...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200737781
更新日期:2008-06-01 00:00:00
abstract::Durch Pflanzenkrankheiten entstehen weltweit hohe Ernteverluste. Eine der häufigsten Infektionen in der Familie der Kreuzb&luml;tengewächse wird durch den Pilz Plasmodiophora brassicae verursacht. Die Krankheit äußert sich in Form von Wurzeltumoren, den "Kohlherniegallen". Da es auf verseuchten Böden zu hohen Ernteaus...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/(sici)1521-415x(200001)30:1<14::aid-biuz14
更新日期:2000-01-01 00:00:00
abstract::In the past 15 years, it became clear that the symbiotic microbiota has an important impact on the development and regulation of the immune system. Consequently, it is incorrect to interpret a phenotype solely as a direct result of the genotype, without considering the impact of the microbiota. In fact, ignorance of t...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.202048615
更新日期:2020-06-01 00:00:00
abstract::Recent studies have suggested that T cell memory for recall antigens resides in clones of primed T cells with a short inter-mitotic half-life. In humans such cells express an isoform of the leukocyte common antigen termed CD45RO. Nevertheless, little is known of the fate of these primed T cells after initial activatio...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830240417
更新日期:1994-04-01 00:00:00
abstract::Accumulating evidence suggest that infection with Chlamydia pneumoniae is associated with atherosclerosis, but the mechanisms involved remain unclear. Inflammation is important in the initial phase of atherogenesis, and cytokines are important in the initiation and progression of inflammation. The aim of this study wa...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/1521-4141(200002)30:2<541::AID-IMMU541>3.0
更新日期:2000-02-01 00:00:00
abstract::Atopic dermatitis (AD) or eczema is the most common chronic inflammatory skin disease. It is a multifactorial disease with local and systemic immune changes. Current therapies focus on restoring the local skin barrier or inhibiting immune responses. In this issue of the European Journal of Immunology, Sehra et al. [Eu...
journal_title:European journal of immunology
pub_type: 评论,杂志文章
doi:10.1002/eji.201646708
更新日期:2016-11-01 00:00:00
abstract::We have proposed earlier that the poor capacity of the lipocalin allergen Bos d 2 to stimulate highly allergic subjects' peripheral blood mononuclear cells could be ascribed to endogenous lipocalins and could be related to the allergenic potential of the molecule. Here, we have characterized the proliferative and cyto...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.200322952
更新日期:2003-06-01 00:00:00
abstract::In the present study, interleukin 1 (IL 1)-containing media from different sources, namely a murine macrophage cell line (P388D1), rabbit peritoneal macrophages, and human peripheral blood mononuclear cells, were compared for their effect on thymocyte proliferation and on collagenase and PGE2 secretion by chondrocytes...
journal_title:European journal of immunology
pub_type: 杂志文章
doi:10.1002/eji.1830140603
更新日期:1984-06-01 00:00:00