Abstract:
:Cotinine biomarker measurements involving both smokers and nonsmokers must accommodate a broad range of concentrations. Thus, we have routinely preclassified unknown samples as being either "high" or "low" by using an enzyme-linked immunoassay for cotinine prior to analysis by tandem mass spectrometry (MS). Although this method is effective, it is also time-consuming and complex; a simpler and faster approach would be useful. Consequently, a screening assay for urine cotinine using an immunochromatographic test strip (NicAlert) followed by a computerized analysis of the data was examined as a possible alternative. The results indicate that this approach can provide useful classification efficiency when using our target cutoff value of approximately 20 ng/mL. In the analysis of 50 urine samples from nonsmokers with varying degrees of exposure to environmental tobacco smoke, the classification sensitivity and specificity were 88% and 92%, respectively, for cotinine measured by the test strips relative to total cotinine concentrations measured by atmospheric-pressure ionization tandem MS. However, the relatively high cost of the strips may be a limiting factor.
journal_name
J Anal Toxicoljournal_title
Journal of analytical toxicologyauthors
Bernert JT,Harmon TL,Sosnoff CS,McGuffey JEdoi
10.1093/jat/29.8.814keywords:
subject
Has Abstractpub_date
2005-11-01 00:00:00pages
814-8issue
8eissn
0146-4760issn
1945-2403journal_volume
29pub_type
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