SEM observation of collagen fibrils secreted from the body surface of osteoblasts on a CO3apatite-collagen sponge.

Abstract:

:The secretion of collagen by osteoblasts was observed by scanning electron microscopy (SEM). Osteoblast-like cells were cultured on a CO3apatite-collagen sponge reinforced with a porous HAp frame. After three days' incubation, a number of newly created matrix fibrils, forming a network structure, were observed at the cell surface. SEM also showed that osteoblasts secreted collagen fibrils from their membrane, and that the collagen fibrils were twisted together. When collagen in an aqueous sol solution was sprayed onto the extremely smooth surface of a mica plate to support the secretion of osteoblasts, a collagen network structure could be clearly observed with atomic force microscopy (AFM). With this in vitro phenomenon, we could confirm the formation of collagen network structure without biological function. Therefore, it was suggested that the CO3apatite-collagen sponge used in this study is a favorable scaffold biomaterial, on which osteoblasts could produce the unmistakable, characteristic extracellular matrix for mineralization. For therapeutic use of hard tissue biomaterials, collagen formation as an extracellular matrix (ECM) is very important because mineralization is subsequent to ECM.

journal_name

Dent Mater J

journal_title

Dental materials journal

authors

Hirata I,Nomura Y,Tabata H,Miake Y,Yanagisawa T,Okazaki M

doi

10.4012/dmj.24.460

keywords:

subject

Has Abstract

pub_date

2005-09-01 00:00:00

pages

460-4

issue

3

eissn

0287-4547

issn

1881-1361

journal_volume

24

pub_type

杂志文章
  • Effect of polishing on cyclic fatigue strength of CAD/CAM ceramics.

    abstract::The biaxial flexural strength and cyclic fatigue biaxial flexural strength of CAD/CAM ceramics polished with #220, 400, 600 and 1000 diamond pads were measured in an effort to determine the effect of surface roughness on fatigue behavior of dental ceramics. The surface roughness was improved after polishing with a sma...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.18.395

    authors: Nakazato T,Takahashi H,Yamamoto M,Nishimura F,Kurosaki N

    更新日期:1999-12-01 00:00:00

  • Possible segregation caused by centrifugal titanium casting.

    abstract::The possibility of the segregation under solidification process using a centrifugal casting machine was investigated using an electron probe microanalyzer with elemental distribution map, line analysis and quantitative analysis. When a very small quantity of platinum was added to local molten titanium during the casti...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.15.212

    authors: Watanabe K,Okawa S,Kanatani M,Nakano S,Miyakawa O,Kobayashi M

    更新日期:1996-12-01 00:00:00

  • Corrosion behavior of dental alloys coupled with titanium. Part 2. The area ratio dependence in five solutions.

    abstract::Sixteen alloys and five pure metals were used and the open circuit potentials (OCP) and the coupled potentials with titanium were measured in five kinds of solutions. All alloys and metals showed the maximum OCP in 1.3% lactic acid solution, and the minimum in 0.01% sodium sulfide solution. The coupled potential depen...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.20.75

    authors: Nakayama M,Ando N

    更新日期:2001-03-01 00:00:00

  • Characterization of 50wt%SiO2-20wt%P2O5-30wt%CaO glass-ceramic powders prepared by the sol-gel process.

    abstract::Glass-ceramic powders with the oxide batch formulation 50wt%SiO2, 20wt%P2O5 and 30wt%CaO were prepared by the sol-gel process using tetraethoxysilane, calcium nitrate and phosphoric acid as raw materials. The obtained gels and powders were characterized by TG-DTA thermal analysis, FTIR analyses and XRD measurements. T...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.16.117

    authors: Taira M,Okazaki M,Takahashi J

    更新日期:1997-12-01 00:00:00

  • Influence of the number of cycles on shear fatigue strength of resin composite bonded to enamel and dentin using dental adhesives in self-etching mode.

    abstract::The influence of the number of cycles on shear fatigue strength to enamel and dentin using dental adhesives in self-etch mode was investigated. A two-step self-etch adhesive and two universal adhesives were used to bond to enamel and dentin in self-etch mode. Initial shear bond strength and shear fatigue strength to e...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2017-012

    authors: Tsujimoto A,Barkmeier WW,Erickson RL,Takamizawa T,Latta MA,Miyazaki M

    更新日期:2018-01-30 00:00:00

  • Effects of CO2 laser irradiation combined with fluoride application on the demineralization, mechanical properties, structure, and composition of enamel.

    abstract::We investigated the effects of CO2 laser irradiation combined with acidulated phosphate fluoride (APF) application on the demineralization of enamel. APF gel was applied to the buccal enamel of human premolars and CO2 laser was applied. After the specimens were immersed in demineralization solution for 72 h, they were...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2014-225

    authors: Nakagaki S,Iijima M,Endo K,Saito T,Mizoguchi I

    更新日期:2015-01-01 00:00:00

  • Effect of adhesive primer developed exclusively for heat-curing resin on adhesive strength between plastic artificial tooth and acrylic denture base resin.

    abstract::Despite progress in the development of denture base resin and artificial tooth materials, dental clinics are still plagued with artificial teeth falling off the denture base--due to poor bond strength--after denture delivery. Against this background, this study sought to examine the effect and durability of an adhesiv...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.25.75

    authors: Nishigawa G,Maruo Y,Okamoto M,Oki K,Kinuta Y,Minagi S,Irie M,Suzuki K

    更新日期:2006-03-01 00:00:00

  • High temperature characteristics and solidification microstructures of dental metallic materials part I: silver-palladium-copper-gold alloy.

    abstract::Ag-Pd-Cu-Au alloy was subjected to a Thermo-Mechanical Analyzer to investigate high temperature properties up to its liquidus temperature. Microstructural examination and elemental analysis with EPMA were also conducted in the solid/liquid mixture region. The following conclusions were obtained. (1) The solidus temper...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.22.227

    authors: Nagasawa S,Yoshida T,Mizoguchi T,Terashima N,Ito M,Platt JA,Oshida Y

    更新日期:2003-09-01 00:00:00

  • Improving biocompatibility of zirconia surface by incorporating Ca ions.

    abstract::Though zirconia has been used in dental implant fixtures, the biocompatibility of the zirconia surface is not optimal for the surrounding tissue, and many surface modifications have been attempted. We have developed a novel method for improving the biocompatibility of zirconia by incorporating Ca ions. Elemental analy...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2014-199

    authors: Sasaki K,Hayashi T,Asakura M,Ando M,Kawai T,Ban S

    更新日期:2015-01-01 00:00:00

  • Determination of molecular species of calcium salts of MDP produced through decalcification of enamel and dentin by MDP-based one-step adhesive.

    abstract::Enamel and dentin particles were added to an experimental 10-methacryloyloxydecyl dihydrogen phosphate (MDP)-based one-step adhesive to react for 30 s. After enamel and dentin reactants were analyzed using X-ray diffraction (XRD) and phosphorus-31 nuclear magnetic resonance ((31)P NMR) techniques, curve-fitting analys...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2014-260

    authors: Yokota Y,Nishiyama N

    更新日期:2015-01-01 00:00:00

  • Type I collagen grafting on titanium surfaces using low-temperature glow discharge.

    abstract::To improve the bioactivity of titanium surfaces, glow discharge was used to facilitate collagen grafting on titanium disks. Titanium test specimens were pre-treated by glow discharge fed with a mixture of argon and allylamine (AA) gases. Treated titanium disks were then grafted with type I collagen using glutaraldehyd...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.27.340

    authors: Chang WJ,Ou KL,Lee SY,Chen JY,Abiko Y,Lin CT,Huang HM

    更新日期:2008-05-01 00:00:00

  • A biocompatible model for evaluation of the responses of rat periapical tissue to a new zinc oxide-eugenol sealer.

    abstract::We aimed to establish an experimental animal model to evaluate materials for endodontic therapy. We focused on the biocompatibility of new paste-type zinc oxide-eugenol (ZOE) sealer. The results of this sealer were compared with those of conventional powder/liquid ZOE and eugenol-free sealers. The molars of Wistar rat...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2010-095

    authors: Mutoh N,Tani-Ishii N

    更新日期:2011-01-01 00:00:00

  • Long-term clinical and radiographic evaluation of the effectiveness of direct pulp capping materials: A meta-analysis.

    abstract::The aim of this review is to evaluate the long-term effectiveness of calcium silicate-based cement (CS) and calcium hydroxide (CH) for direct pulp capping (DPC) to human pulp-exposed permanent teeth. An electronic search and manual search were performed on 21 June 2019. Long-term clinical and radiographic evaluations ...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2020-043

    authors: Matsuura T,Ziauddin SM,Kawata-Matsuura VKS,Sugimoto K,Yamada S,Yoshimura A

    更新日期:2020-10-01 00:00:00

  • Influence of three specimen fixation modes on the micro-tensile bond strength of adhesives to dentin.

    abstract::The aim of this study was to investigate in how far the way the specimen is fixed to the testing device influences the micro-tensile bond strength of adhesives to dentin. Compared to a flat jig, a notched jig enables the specimen to be aligned easier and more accurately perpendicular to the interface, thereby concentr...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.26.694

    authors: Poitevin A,De Munck J,Van Landuyt K,Coutinho E,Peumans M,Lambrechts P,Van Meerbeek B

    更新日期:2007-09-01 00:00:00

  • Microtensile bond strength of a newly developed resin cement to dentin.

    abstract::The purpose of this study was to evaluate the microtensile bond strength (µTBS) of a newly developed resin cement, ECD-89 (ECD, Tokuyama Dental, Tokyo, Japan) to dentin and to observe the interfacial micromorphology by comparing with two commercial resin cements, Multilink Automix (MA, Ivoclar Vivadent AG, Schaan, Lie...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2014-122

    authors: Kawano S,Fu J,Saikaew P,Chowdhury AA,Fukuzawa N,Kadowaki Y,Kakuda S,Hoshika S,Nakaoki Y,Ikeda T,Tanaka T,Sano H

    更新日期:2015-01-01 00:00:00

  • Chemical mechanical polishing of titanium with colloidal silica containing hydrogen peroxide--mirror polishing and surface properties.

    abstract::Chemical mechanical polishing (CMP) of cpTi (Ti) was carried out using two types of slurries, acidic and basic colloidal silica containing H2O2 up to 3 wt%, to obtain flat and mirror-polished surfaces without any contaminated and reacted layers. Polishing behavior and surface properties were investigated using AFM, EP...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.28.68

    authors: Okawa S,Watanabe K

    更新日期:2009-01-01 00:00:00

  • Effect of Cr and Cu addition on corrosion behavior of Ni-Ti alloys.

    abstract::The corrosion behavior of three Ni-Ti alloys with compositions as commercial super-elastic orthodontic wires was investigated using polished plate specimens. Corrosion resistance was estimated by potentiodynamic polarization measurement in 0.9% NaCl and 1% lactic acid solutions and analysis of released metals by atomi...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.17.31

    authors: Iijima M,Endo K,Ohno H,Mizoguchi I

    更新日期:1998-03-01 00:00:00

  • Experimental studies on the application of a porcelain-light-cured resin matrix system to the porcelain margin of metal-ceramic crowns.

    abstract::Application of a light-cured porcelain margin is a technique which has been developed recently. In this study, we compared the porcelain margin properties of the porcelain-light-cured resin matrix system and the traditional porcelain-water system, in terms of volumetric shrinkage, density, compressive strength, surfac...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.14.1

    authors: Lu YS,Yang HW

    更新日期:1995-06-01 00:00:00

  • A novel glass ionomer cement containing MgCO(3 )apatite induced the increased proliferation and differentiation of human pulp cells in vitro.

    abstract::This study aimed to investigate the in vitro biological response of human dental pulp cells to glass ionomer cement (GIC, Fuji IX GP(®)) containing 2.5% magnesium carbonate apatite (MgCO(3)Ap). MgCO(3)Ap was synthesized by wet method and characterized using FT-IR, XPS, and SEM. Fuji IX GP(®) served as a control. Test ...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2012-096

    authors: Laiteerapong A,Lochaiwatana Y,Hirata I,Okazaki M,Mori K,Murakami S,Poolthong S

    更新日期:2012-01-01 00:00:00

  • Predicting in vivo failure of rotary nickel-titanium endodontic instruments under cyclic fatigue.

    abstract::The aim of this study was to examine the lifespan or number of cycles to failure of tapered rotary nickel-titanium (Ni-Ti) endodontic instruments. Simulated root canals with different curvatures were used to determine a relation between canal curvature and instrument lifespan. Using a novel mathematical model for the ...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2011-254

    authors: Stojanac I,Drobac M,Petrovic L,Atanackovic T

    更新日期:2012-01-01 00:00:00

  • Effect of incremental filling technique on adhesion of light-cured resin composite to cavity floor.

    abstract::The purpose of this study was to evaluate the effect of various incremental filling techniques on adhesion between composite and cavity floor using light-cured resin composite. Black ABS resin and hybrid resin composite were used as mold materials--instead of dentin--for the preparation of cavities, and standardized t...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.25.503

    authors: Chikawa H,Inai N,Cho E,Kishikawa R,Otsuki M,Foxton RM,Tagami J

    更新日期:2006-09-01 00:00:00

  • Bactericidal efficacy of glycine-type amphoteric surfactant as a denture cleaner and its influence on properties of denture base resins.

    abstract::The bactericidal efficacy of 1.00-4.50% glycine-type amphoteric surfactant (Gly) was evaluated by measuring its microorganism removal rate in denture plaque. Physical and mechanical properties such as surface roughness, color difference, and bending strength of two different denture base resins were determined before ...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.28.307

    authors: Hashiguchi M,Nishi Y,Kanie T,Ban S,Nagaoka E

    更新日期:2009-05-01 00:00:00

  • Surface damages of zirconia by Nd:YAG dental laser irradiation.

    abstract::The purpose of this study was to characterize the surface damages of zirconia by Nd:YAG dental laser irradiation through a systematic evaluation of the associated microstructural changes. Disk specimens of yttria-stabilized tetragonal zirconia polycrystals (Y-TZP) and ceria-stabilized zirconia/alumina nanocomposite (C...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2009-127

    authors: Noda M,Okuda Y,Tsuruki J,Minesaki Y,Takenouchi Y,Ban S

    更新日期:2010-10-01 00:00:00

  • Wear resistance of hybrid composite resin for crown material by the two-body sliding test.

    abstract::Comparative studies on two-body sliding wear resistance were performed between a hybrid composite resin and conventional light-curing composite veneering materials. This study investigated the wear resistance of hybrid composite resins compared with three composite resin veneering materials for 12%Au-Ag-Pd alloy, the ...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.21.225

    authors: Suese K,Kawazoe T

    更新日期:2002-09-01 00:00:00

  • Effect of monomer structure on the mechanical properties of light-cured composite resins.

    abstract::The effects of monomer structure on the mechanical properties of visible light-cured composite resins based on the seven types of aromatic dimethacrylates were investigated. The results of this study suggested that the mechanical properties of the composite resins were dependent upon the chemical structure of the dime...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.8.40

    authors: Kawaguchi M,Fukushima T,Horibe T

    更新日期:1989-06-01 00:00:00

  • The hydration chemistry of ProRoot MTA.

    abstract::'Tooth-coloured' ProRoot MTA is an endodontic cement comprising an 80:20 wt% mixture of white Portland cement (WPC) and bismuth oxide. The setting reactions within this cement system are not currently well understood. Accordingly, this research monitors the early hydration chemistry of ProRoot MTA by X-ray diffraction...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2014-309

    authors: Li Q,Coleman NJ

    更新日期:2015-01-01 00:00:00

  • Effects of fluoride and dissolved oxygen concentrations on the corrosion behavior of pure titanium and titanium alloys.

    abstract::The effects of dissolved-oxygen concentration and fluoride concentration on the corrosion behaviors of commercial pure titanium, Ti-6Al-4V and Ti-6Al-7Nb alloys and experimentally produced Ti-0.2Pd and Ti-0.5Pt alloys were examined using the corrosion potential measurements. The amount of dissolved Ti was analyzed by ...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.21.83

    authors: Nakagawa M,Matsuya S,Udoh K

    更新日期:2002-06-01 00:00:00

  • In vivo analyses of the effects of polyamidoamine dendrimer on dentin biomineralization and dentinal tubules occlusion.

    abstract::This study evaluated the biomineralization and dentinal tubules occlusion abilities of the carboxyl-terminated polyamidoamine dendrimer (PAMAM-COOH) on human demineralized dentin in vivo at different time points. Demineralization dentin model with and without treated with PAMAM-COOH were sutured to the interior side o...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2015-209

    authors: Xie F,Wei X,Li Q,Zhou T

    更新日期:2016-01-01 00:00:00

  • Response of osteoblast-like cells to zirconia with different surface topography.

    abstract::The objective of this study was to clarify the effect of surface topography on osteoblast-like cell behavior on yttria stabilized tetragonal zirconia polycrystals (TZP). Mirror-polished; blasted with 50- or 150-µm alumina (SB50 and SB150); and SB150 acid-etched with hydrofluoric acid (SB150E) were prepared on TZP. Ini...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.2012-208

    authors: Ito H,Sasaki H,Saito K,Honma S,Yajima Y,Yoshinari M

    更新日期:2013-01-01 00:00:00

  • Effect of commercially available bonding agents impregnated with fibers on bending strength of hybrid resin.

    abstract::To clarify the mechanical properties of fiber-reinforced hybrid resin bridges, this study evaluated the influence of various bonding agents ((Modeling Liquid (ML), DE Resin (DE), Bell Bond (BE), Mega Bond (MG), Durafil Bond (DU), Fluoro Bond (FB), Mac-Bond (MC), EG Bond (EG), Unifill Bond (UN), Single Bond (SN)) impre...

    journal_title:Dental materials journal

    pub_type: 杂志文章

    doi:10.4012/dmj.27.723

    authors: Tsushima S,Gomi H,Shinya A,Yokoyama D,Vallittu PK,Shinya A

    更新日期:2008-09-01 00:00:00