Abstract:
:This study aimed to investigate the in vitro biological response of human dental pulp cells to glass ionomer cement (GIC, Fuji IX GP(®)) containing 2.5% magnesium carbonate apatite (MgCO(3)Ap). MgCO(3)Ap was synthesized by wet method and characterized using FT-IR, XPS, and SEM. Fuji IX GP(®) served as a control. Test and control cements were prepared by encapsulated mixing the powder with Fuji IX-liquid (P/L=3.6:1). Eluates from cements extracted by 1 mL culture medium were collected at day 1, 7 and 14, and used for WST-1 proliferation assay. For ALPase activity, cells were maintained with cements in transwells, harvested and enzyme activity was measured at day 1, 4, 7, 14, and 21. We found a higher cell proliferation and increased ALPase activity by pulp cells in the test group compared to the control. This suggests the potential of GIC containing this novel biological apatite as a restorative material for pulp-dentin regeneration.
journal_name
Dent Mater Jjournal_title
Dental materials journalauthors
Laiteerapong A,Lochaiwatana Y,Hirata I,Okazaki M,Mori K,Murakami S,Poolthong Sdoi
10.4012/dmj.2012-096subject
Has Abstractpub_date
2012-01-01 00:00:00pages
772-8issue
5eissn
0287-4547issn
1881-1361pii
DN/JST.JSTAGE/dmj/2012-096journal_volume
31pub_type
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