A novel glass ionomer cement containing MgCO(3 )apatite induced the increased proliferation and differentiation of human pulp cells in vitro.

Abstract:

:This study aimed to investigate the in vitro biological response of human dental pulp cells to glass ionomer cement (GIC, Fuji IX GP(®)) containing 2.5% magnesium carbonate apatite (MgCO(3)Ap). MgCO(3)Ap was synthesized by wet method and characterized using FT-IR, XPS, and SEM. Fuji IX GP(®) served as a control. Test and control cements were prepared by encapsulated mixing the powder with Fuji IX-liquid (P/L=3.6:1). Eluates from cements extracted by 1 mL culture medium were collected at day 1, 7 and 14, and used for WST-1 proliferation assay. For ALPase activity, cells were maintained with cements in transwells, harvested and enzyme activity was measured at day 1, 4, 7, 14, and 21. We found a higher cell proliferation and increased ALPase activity by pulp cells in the test group compared to the control. This suggests the potential of GIC containing this novel biological apatite as a restorative material for pulp-dentin regeneration.

journal_name

Dent Mater J

journal_title

Dental materials journal

authors

Laiteerapong A,Lochaiwatana Y,Hirata I,Okazaki M,Mori K,Murakami S,Poolthong S

doi

10.4012/dmj.2012-096

subject

Has Abstract

pub_date

2012-01-01 00:00:00

pages

772-8

issue

5

eissn

0287-4547

issn

1881-1361

pii

DN/JST.JSTAGE/dmj/2012-096

journal_volume

31

pub_type

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