Abstract：
:AFTER exposure to secretagogues the small intestine changes from a tissue that absorbs fluid and electrolyte from lumen to blood into a tissue that secretes electrolyte and fluid into the lumen(1-4). It has been shown that this secretion results from an increase in the passive Cl(-) permeability of the mucosal border, which permits Nad to leak passively from the lateral intercellular spaces, where it is present at hypertonic concentrations(5), into the mucosal bathing solution. Na(+) and water, electroosmotically coupled to Na(+) movement, leak through the tight junctions(1,2), and Cl(-) leaks through relatively anhydrous anion-selective channels, induced withira the mucosal border by secretagogues. The increased reflux of NaCl from the lateral intercellular space accounts for both the apparent decrease in electroneutral NaCl uptake across the mucosal border induced by secretagogues and the apparent increase in active CP secretion and short-circuit current(3,6,7). We have investigated the mechanism by which intestinal secretagogues increase passive Cl(-) permeability and thereby cause secretion. Cl(-) permeability is increased by several secretagogues, some of which, such as theophylline and choleragen, increase intracellular cyclic AMP concentration, and others, such as A23187, the Ca(2+) ionophore, or carbachol, do not(8). Thus there has been no known common mode of secretory induction. To investigate this problem we used two drugs that prevent intestinal secretion in vitro, RMI 12330A (Richardson Merrell), and the antipsychotic pheno-thiazine trifluoperazine (Stelazine, Smith, Kline and French). RMI 12330A prevents secretion by inhibiting choleragen-induced adenylyl cyclase activity(9). Stelazine inhibits phosphodiesterase in tissues(11,12) by preventing the activation of the enzyme by Ca(2+)-dependent regulator protein, CDR. We report here that it also inhibits Cl(-) secretion and binds to CDR.
journal_name
Naturejournal_title
Natureauthors
Ilundain A,Naftalin RJdoi
10.1038/279446a0keywords:
subject
Has Abstractpub_date
1979-05-31 00:00:00pages
446-8issue
5712eissn
0028-0836issn
1476-4687pii
279446a0journal_volume
279pub_type
杂志文章相关文献
NATURE文献大全abstract：:Transport vesicles derived from the Golgi apparatus are thought to mediate biosynthetic transport across the Golgi stack. These vesicles are surrounded by a protein coat whose principal constituents are coatomer (a complex of seven distinct subunits or COPs) and ADP-ribosylation factor (ARF, an N-myristylated small GT...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/364732a0
更新日期：1993-08-19 00:00:00
abstract：:Genetic testing will become the future standard of medical care. Life insurers will also need access to genetic information if the insurance industry is to survive intact and if cover is to remain affordable. ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/376013a0
更新日期：1995-07-06 00:00:00
abstract：:We have developed a method for calculating the stability in water of protein structures, starting from their atomic coordinates. The contribution of each protein atom to the solvation free energy is estimated as the product of the accessibility of the atom to solvent and its atomic solvation parameter. Applications of...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/319199a0
更新日期：1986-01-16 00:00:00
abstract：:The beta, gamma-crystallins form a class of homologous proteins in the eye lens. Each gamma-crystallin comprises four topologically equivalent, Greek key motifs; pairs of motifs are organized around a local dyad to give domains and two similar domains are in turn related by a further local dyad. Sequence comparisons a...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/347776a0
更新日期：1990-10-25 00:00:00
abstract：:Carbon exchange between the terrestrial biosphere and the atmosphere is one of the key processes that need to be assessed in the context of the Kyoto Protocol. Several studies suggest that the terrestrial biosphere is gaining carbon, but these estimates are obtained primarily by indirect methods, and the factors that ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/35009084
更新日期：2000-04-20 00:00:00
abstract：:Many-body systems in nature exhibit complexity and self-organization arising from seemingly simple laws. For example, the long-range Coulomb interaction between electrical charges has a simple form, yet is responsible for a plethora of bound states in matter, ranging from the hydrogen atom to complex biochemical struc...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature01676
更新日期：2003-06-12 00:00:00
abstract：:A hybrid gene was constructed between the beta-lactamase gene of plasmid pBR322 and the cloned coding sequence for rat growth hormone. This gene is expressed in bacteria and growth hormone sequences are detectable by immunological methods. ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/276795a0
更新日期：1978-12-21 00:00:00
abstract：:A putative growth hormone receptor from rabbit liver and the growth hormone binding protein from rabbit serum have the same amino-terminal amino-acid sequence, indicating that the binding protein corresponds to the extracellular hormone-binding domain of the liver receptor. The complete amino-acid sequences derived fr...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/330537a0
更新日期：1987-12-10 00:00:00
abstract：:Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), a much milder form of the disease where the age of onset can sometimes be as late as the third or fourth decade of life, are caused by mutations in the same X-linked gene, a 14 kilobase (kb) transcript which is spread over more than 2 megabases of ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/343180a0
更新日期：1990-01-11 00:00:00
abstract：:Understanding the determinants of healthy mental ageing is a priority for society today. So far, we know that intelligence differences show high stability from childhood to old age and there are estimates of the genetic contribution to intelligence at different ages. However, attempts to discover whether genetic cause...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature10781
更新日期：2012-01-18 00:00:00
abstract：:Hypermethylation is associated with the silencing of tumour susceptibility genes in several forms of cancer; however, the mechanisms responsible for this aberrant methylation are poorly understood. The prototypic DNA methyltransferase, DNMT1, has been widely assumed to be responsible for most of the methylation of the...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/35010000
更新日期：2000-04-27 00:00:00
abstract：:The cosmic radio-frequency spectrum is expected to show a strong absorption signal corresponding to the 21-centimetre-wavelength transition of atomic hydrogen around redshift 20, which arises from Lyman-α radiation from some of the earliest stars. By observing this 21-centimetre signal-either its sky-averaged spectrum...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature25791
更新日期：2018-02-28 00:00:00
abstract：:Endogenous retroviruses (ERVs) are abundant and heterogenous groups of integrated retroviral sequences that affect genome regulation and cell physiology throughout their RNA-centred life cycle1. Failure to repress ERVs is associated with cancer, infertility, senescence and neurodegenerative diseases2,3. Here, using an...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-020-03135-1
更新日期：2021-01-13 00:00:00
abstract：:One approach to the controlled release of drugs involves incorporation of the drug molecules into the matrix of microscopic polymer spheres or capsules. Existing methods for preparing such microparticles do not, however, always guarantee a constant release rate, for example because drug molecules may be trapped prefer...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/367258a0
更新日期：1994-01-20 00:00:00
abstract：:Endocytosis has long been thought of as simply a way for cells to internalize nutrients and membrane-associated molecules. But an explosive growth in knowledge has given a new dimension to our understanding of this process. It now seems that endocytosis is a master organizer of signalling circuits, with one of its mai...
journal_title：Nature
pub_type： 杂志文章,评审
doi：10.1038/nature08910
更新日期：2010-01-28 00:00:00
abstract：:It is thought that neutron stars in low-mass binary systems can accrete matter and angular momentum from the companion star and be spun-up to millisecond rotational periods. During the accretion stage, the system is called a low-mass X-ray binary, and bright X-ray emission is observed. When the rate of mass transfer d...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature12470
更新日期：2013-09-26 00:00:00
abstract：:The masses of the supermassive black holes found in galaxy bulges are correlated with a multitude of galaxy properties, leading to suggestions that galaxies and black holes may evolve together. The number of reliably measured black-hole masses is small, and the number of methods for measuring them is limited, holding ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature11819
更新日期：2013-02-21 00:00:00
abstract：:One reason for the failure of chemotherapy in the treatment of advanced cancers may be the outgrowth of multidrug-resistant tumour cells. Multidrug resistance has been modelled in numerous mammalian cell lines in which the phenotype is characterized by a pleiotropic cross-resistance to unrelated drugs. In the study re...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/316820a0
更新日期：1985-08-29 00:00:00
abstract：:The yeast [PSI+] element represents a new type of genetic inheritance, in which changes in phenotype are transmitted by a 'protein only' mechanism reminiscent of the 'protein-only' transmission of mammalian prion diseases. The underlying molecular mechanisms for both are poorly understood and it is not clear how simil...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/23048
更新日期：1999-08-05 00:00:00
abstract：:The gut of healthy human neonates is usually devoid of viruses at birth, but quickly becomes colonized, which-in some cases-leads to gastrointestinal disorders1-4. Here we show that the assembly of the viral community in neonates takes place in distinct steps. Fluorescent staining of virus-like particles purified from...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-020-2192-1
更新日期：2020-05-01 00:00:00
abstract：:The ability to process microbial antigens and present them at the surface of cells is an important aspect of our innate ability to clear infections. It is generally accepted that antigens in the cytoplasm are loaded in the endoplasmic reticulum and presented at the cell surface on major histocompatibility complex (MHC...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature01912
更新日期：2003-09-25 00:00:00
abstract：:'Identifier' or ID sequences are present in 62% of the RNA polymerase II and III transcripts made in vitro from brain nuclei but in fewer than 4% of the transcripts made from the nuclei of other tissues. An homologous 160-nucleotide cytoplasmic poly(A)+ RNA species, BC1, and a smaller species, BC2, are located in vivo...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/308237a0
更新日期：1984-03-15 00:00:00
abstract：:Current ideas about the dependence of neurons on target-derived growth factors were formulated on the basis of experiments involving neurons with projections to the periphery. Nerve growth factor (NGF) and recently identified members of the NGF family of neuronal growth factors, known as neurotrophins, are thought to ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/360753a0
更新日期：1992-12-24 00:00:00
abstract：:The epiblast (EPI) is the origin of all somatic and germ cells in mammals, and of pluripotent stem cells in vitro. To explore the ontogeny of human and primate pluripotency, here we perform comprehensive single-cell RNA sequencing for pre- and post-implantation EPI development in cynomolgus monkeys (Macaca fasciculari...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature19096
更新日期：2016-09-01 00:00:00
abstract：:Shiga toxin and some other protein toxins that act on targets in the cytosol have previously been shown to enter the trans-Golgi network. Transport by this route may be necessary for translocation of the toxin to the cytosol and for intoxication, but it is not known whether the enzymatically active part of the toxins ...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/358510a0
更新日期：1992-08-06 00:00:00
abstract：:Silicon dominates contemporary solar cell technologies1. But when absorbing photons, silicon (like other semiconductors) wastes energy in excess of its bandgap2. Reducing these thermalization losses and enabling better sensitivity to light is possible by sensitizing the silicon solar cell using singlet exciton fission...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-019-1339-4
更新日期：2019-07-01 00:00:00
abstract：:In eukaryotes, accurate chromosome segregation in mitosis and meiosis maintains genome stability and prevents aneuploidy. Kinetochores are large protein complexes that, by assembling onto specialized Cenp-A nucleosomes1,2, function to connect centromeric chromatin to microtubules of the mitotic spindle3,4. Whereas the...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/s41586-019-1609-1
更新日期：2019-10-01 00:00:00
abstract：:RNA ligases have essential roles in many cellular processes in eukaryotes, archaea and bacteria, including in RNA repair and stress-induced splicing of messenger RNA. In archaea and eukaryotes, RNA ligases also have a role in transfer RNA splicing to generate functional tRNAs required for protein synthesis. We recentl...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/nature13284
更新日期：2014-07-03 00:00:00
abstract：:The AP-1 family of transcription factors, which includes the proto-oncogene products c-Jun and c-Fos, controls the stimulation of cellular genes by growth factors and the expression of oncogenes, including src and ras. Transcriptional activation by c-Jun is regulated by a cell-type-specific inhibitor that represses th...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/352165a0
更新日期：1991-07-11 00:00:00
abstract：:The shape of the human erythrocyte depends on intracellular ATP content and echinocytic erythrocyte ghosts obtained in the presence of Mg-ATP acquire a diskocytic shape after incubation at 37 degrees C; however, agreement is lacking about the molecular basis of the shape changes. The suggestion that phosphorylation of...
journal_title：Nature
pub_type： 杂志文章
doi：10.1038/307376a0
更新日期：1984-01-26 00:00:00
