Synthesis of growth hormone by bacteria.


:A hybrid gene was constructed between the beta-lactamase gene of plasmid pBR322 and the cloned coding sequence for rat growth hormone. This gene is expressed in bacteria and growth hormone sequences are detectable by immunological methods.






Seeburg PH,Shine J,Martial JA,Ivarie RD,Morris JA,Ullrich A,Baxter JD,Goodman HM




Has Abstract


1978-12-21 00:00:00














  • Noise-resistant and synchronized oscillation of the segmentation clock.

    abstract::Periodic somite segmentation in vertebrate embryos is controlled by the 'segmentation clock', which consists of numerous cellular oscillators. Although the properties of a single oscillator, driven by a hairy negative-feedback loop, have been investigated, the system-level properties of the segmentation clock remain l...


    pub_type: 杂志文章


    authors: Horikawa K,Ishimatsu K,Yoshimoto E,Kondo S,Takeda H

    更新日期:2006-06-08 00:00:00

  • Substantial reservoirs of molecular hydrogen in the debris disks around young stars.

    abstract::Circumstellar accretion disks transfer matter from molecular clouds to young stars and to the sites of planet formation. The disks observed around pre-main-sequence stars have properties consistent with those expected for the pre-solar nebula from which our own Solar System formed 4.5 Gyr ago. But the 'debris' disks t...


    pub_type: 杂志文章


    authors: Thi WF,Blake GA,van Dishoeck EF,van Zadelhoff GJ,Horn JM,Becklin EE,Mannings V,Sargent AI,van Den Ancker ME,Natta A

    更新日期:2001-01-04 00:00:00

  • Quantum phase transition in a common metal.

    abstract::The classical theory of solids, based on the quantum mechanics of single electrons moving in periodic potentials, provides an excellent description of substances ranging from semiconducting silicon to superconducting aluminium. Over the last fifteen years, it has become increasingly clear that there are substances for...


    pub_type: 杂志文章


    authors: Yeh A,Soh YA,Brooke J,Aeppli G,Rosenbaum TF,Hayden SM

    更新日期:2002-10-03 00:00:00

  • Normalizing the environment recapitulates adult human immune traits in laboratory mice.

    abstract::Our current understanding of immunology was largely defined in laboratory mice, partly because they are inbred and genetically homogeneous, can be genetically manipulated, allow kinetic tissue analyses to be carried out from the onset of disease, and permit the use of tractable disease models. Comparably reductionist ...


    pub_type: 杂志文章


    authors: Beura LK,Hamilton SE,Bi K,Schenkel JM,Odumade OA,Casey KA,Thompson EA,Fraser KA,Rosato PC,Filali-Mouhim A,Sekaly RP,Jenkins MK,Vezys V,Haining WN,Jameson SC,Masopust D

    更新日期:2016-04-28 00:00:00

  • Topoisomerase II mediates meiotic crossover interference.

    abstract::Spatial patterning is a ubiquitous feature of biological systems. Meiotic crossovers provide an interesting example, defined by the classic phenomenon of crossover interference. Here we identify a molecular pathway for interference by analysing crossover patterns in budding yeast. Topoisomerase II plays a central role...


    pub_type: 杂志文章


    authors: Zhang L,Wang S,Yin S,Hong S,Kim KP,Kleckner N

    更新日期:2014-07-31 00:00:00

  • Conservation of hippocampal memory function in rats and humans.

    abstract::The hippocampus is critical to declarative memory in humans. This kind of memory involves associations among items or events that can be accessed flexibly to guide memory expression in various and even new situations. In animals, there has been controversy about whether the hippocampus is specialized for spatial memor...


    pub_type: 杂志文章


    authors: Bunsey M,Eichenbaum H

    更新日期:1996-01-18 00:00:00

  • The PIE-1 protein and germline specification in C. elegans embryos.

    abstract::Totipotent germline blastomeres in Caenorhabditis elegans contain, but do not respond to, factors that promote somatic differentiation in other embryonic cells. Mutations in the maternal gene pie-1 result in the germline blastomeres adopting somatic cell fates. Here we show that pie-1 encodes a nuclear protein, PIE-1,...


    pub_type: 信件


    authors: Mello CC,Schubert C,Draper B,Zhang W,Lobel R,Priess JR

    更新日期:1996-08-22 00:00:00

  • Macrocortin: a polypeptide causing the anti-phospholipase effect of glucocorticoids.

    abstract::Anti-inflammatory glucocorticoids inhibit prostaglandin (PG) biosynthesis by preventing arachidonic acid release from phospholipids rather than inhibiting the cyclooxygenase. As in other cells, this steroid action depends on receptor occupation and de novo protein/RNA biosynthesis. We have previously shown in guinea p...


    pub_type: 杂志文章


    authors: Blackwell GJ,Carnuccio R,Di Rosa M,Flower RJ,Parente L,Persico P

    更新日期:1980-09-11 00:00:00

  • A seismic reflection image for the base of a tectonic plate.

    abstract::Plate tectonics successfully describes the surface of Earth as a mosaic of moving lithospheric plates. But it is not clear what happens at the base of the plates, the lithosphere-asthenosphere boundary (LAB). The LAB has been well imaged with converted teleseismic waves, whose 10-40-kilometre wavelength controls the s...


    pub_type: 杂志文章


    authors: Stern TA,Henrys SA,Okaya D,Louie JN,Savage MK,Lamb S,Sato H,Sutherland R,Iwasaki T

    更新日期:2015-02-05 00:00:00

  • Blazar spectral variability as explained by a twisted inhomogeneous jet.

    abstract::Blazars are active galactic nuclei, which are powerful sources of radiation whose central engine is located in the core of the host galaxy. Blazar emission is dominated by non-thermal radiation from a jet that moves relativistically towards us, and therefore undergoes Doppler beaming. This beaming causes flux enhancem...


    pub_type: 杂志文章


    authors: Raiteri CM,Villata M,Acosta-Pulido JA,Agudo I,Arkharov AA,Bachev R,Baida GV,Benítez E,Borman GA,Boschin W,Bozhilov V,Butuzova MS,Calcidese P,Carnerero MI,Carosati D,Casadio C,Castro-Segura N,Chen WP,Damljanovic G,D'

    更新日期:2017-12-21 00:00:00

  • Curare can open and block ionic channels associated with cholinergic receptors.

    abstract::Curare has long been regarded as a typical competitive antagonist of acetylcholine (ACh) at the vertebrate neuromuscular junction. Recently, however, it has been shown that curare can also block the channels opened by ACh at the frog neuromuscular junction as well as on rat and Aplysia neurones; moreover, curare is ab...


    pub_type: 杂志文章


    authors: Trautmann A

    更新日期:1982-07-15 00:00:00

  • Origin of a 'Southern Hemisphere' geochemical signature in the Arctic upper mantle.

    abstract::The Gakkel ridge, which extends under the Arctic ice cap for approximately 1,800 km, is the slowest spreading ocean ridge on Earth. Its spreading created the Eurasian basin, which is isolated from the rest of the oceanic mantle by North America, Eurasia and the Lomonosov ridge. The Gakkel ridge thus provides unique op...


    pub_type: 杂志文章


    authors: Goldstein SL,Soffer G,Langmuir CH,Lehnert KA,Graham DW,Michael PJ

    更新日期:2008-05-01 00:00:00

  • Stage-specific control of neuronal migration by somatostatin.

    abstract::Developing neurons transiently express somatostatin and its receptors, but little is known about their function at these early stages. As we thought that endogenous somatostatin might control the migratory behaviour of immature neurons, we have examined the effects of somatostatin in cerebellar granule cells of early ...


    pub_type: 杂志文章


    authors: Yacubova E,Komuro H

    更新日期:2002-01-03 00:00:00

  • Retrograde axonal transport of ciliary neurotrophic factor is increased by peripheral nerve injury.

    abstract::Ciliary neurotrophic factor (CNTF) promotes the survival of several populations of neurons, including sensory and motor neurons. Although CNTF is abundant in adult sciatic nerve, the mature protein lacks a signal sequence and is not secreted; therefore, it has been proposed to act as a lesion factor. The identificatio...


    pub_type: 杂志文章


    authors: Curtis R,Adryan KM,Zhu Y,Harkness PJ,Lindsay RM,DiStefano PS

    更新日期:1993-09-16 00:00:00

  • Monoclonal antibody production by receptor-mediated electrically induced cell fusion.

    abstract::Fusion of myeloma cells and B lymphocytes to form hybridomas which produce monoclonal antibodies has been a major advance, but the poor efficiency and randomness of viral or polyethylene glycol fusion techniques generally gives poor yields of specific, high affinity antibodies. High voltage electrical fields with diel...


    pub_type: 杂志文章


    authors: Lo MM,Tsong TY,Conrad MK,Strittmatter SM,Hester LD,Snyder SH

    更新日期:1984-08-30 00:00:00

  • Single-species models for many-species food webs.

    abstract::Most species live in species-rich food webs; yet, for a century, most mathematical models for population dynamics have included only one or two species. We ask whether such models are relevant to the real world. Two-species population models of an interacting consumer and resource collapse to one-species dynamics when...


    pub_type: 杂志文章


    authors: Murdoch WW,Kendall BE,Nisbet RM,Briggs CJ,McCauley E,Bolser R

    更新日期:2002-05-30 00:00:00

  • Non-equivalent contributions of maternal and paternal genomes to early plant embryogenesis.

    abstract::Zygotic genome activation in metazoans typically occurs several hours to a day after fertilization, and thus maternal RNAs and proteins drive early animal embryo development. In plants, despite several molecular studies of post-fertilization transcriptional activation, the timing of zygotic genome activation remains a...


    pub_type: 杂志文章


    authors: Del Toro-De León G,García-Aguilar M,Gillmor CS

    更新日期:2014-10-30 00:00:00

  • Insulin action impaired by deficiency of the G-protein subunit G ialpha2.

    abstract::Integration of information between tyrosine kinase and G-protein-mediated pathways is necessary, but remains poorly understood. Here we use cells from transgenic mice harbouring inducible expression of RNA antisense to the gene encoding G ialpha2 to show that G ialpha2 is critical for insulin action. G ialpha2 deficie...


    pub_type: 杂志文章


    authors: Moxham CM,Malbon CC

    更新日期:1996-02-29 00:00:00

  • Australian tropical cyclone activity lower than at any time over the past 550-1,500 years.

    abstract::The assessment of changes in tropical cyclone activity within the context of anthropogenically influenced climate change has been limited by the short temporal resolution of the instrumental tropical cyclone record (less than 50 years). Furthermore, controversy exists regarding the robustness of the observational reco...


    pub_type: 历史文章,杂志文章


    authors: Haig J,Nott J,Reichart GJ

    更新日期:2014-01-30 00:00:00

  • Modelled atmospheric temperatures and global sea levels over the past million years.

    abstract::Marine records of sediment oxygen isotope compositions show that the Earth's climate has gone through a succession of glacial and interglacial periods during the past million years. But the interpretation of the oxygen isotope records is complicated because both isotope storage in ice sheets and deep-water temperature...


    pub_type: 历史文章,杂志文章


    authors: Bintanja R,van de Wal RS,Oerlemans J

    更新日期:2005-09-01 00:00:00

  • Clinical efficacy of a RAF inhibitor needs broad target blockade in BRAF-mutant melanoma.

    abstract::B-RAF is the most frequently mutated protein kinase in human cancers. The finding that oncogenic mutations in BRAF are common in melanoma, followed by the demonstration that these tumours are dependent on the RAF/MEK/ERK pathway, offered hope that inhibition of B-RAF kinase activity could benefit melanoma patients. He...


    pub_type: 杂志文章


    authors: Bollag G,Hirth P,Tsai J,Zhang J,Ibrahim PN,Cho H,Spevak W,Zhang C,Zhang Y,Habets G,Burton EA,Wong B,Tsang G,West BL,Powell B,Shellooe R,Marimuthu A,Nguyen H,Zhang KY,Artis DR,Schlessinger J,Su F,Higgins B,Iy

    更新日期:2010-09-30 00:00:00

  • Light-inducible and chloroplast-associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector.

    abstract::A chimaeric gene, consisting of the 5'-flanking region of a member of the Pisum sativum gene family encoding ribulose 1,5-bisphosphate carboxylase linked to the coding region of a bacterial chloramphenicol acetyltransferase gene, has been introduced into the genome of the plant Nicotiana tabacum using a Ti plasmid of ...


    pub_type: 杂志文章


    authors: Herrera-Estrella L,Van den Broeck G,Maenhaut R,Van Montagu M,Schell J,Timko M,Cashmore A

    更新日期:1984-07-12 00:00:00

  • The contribution of China's emissions to global climate forcing.

    abstract::Knowledge of the contribution that individual countries have made to global radiative forcing is important to the implementation of the agreement on "common but differentiated responsibilities" reached by the United Nations Framework Convention on Climate Change. Over the past three decades, China has experienced rapi...


    pub_type: 杂志文章


    authors: Li B,Gasser T,Ciais P,Piao S,Tao S,Balkanski Y,Hauglustaine D,Boisier JP,Chen Z,Huang M,Li LZ,Li Y,Liu H,Liu J,Peng S,Shen Z,Sun Z,Wang R,Wang T,Yin G,Yin Y,Zeng H,Zeng Z,Zhou F

    更新日期:2016-03-17 00:00:00

  • Colombia creates its first science ministry.

    abstract:: ...


    pub_type: 新闻


    authors: Wight AJ

    更新日期:2019-01-01 00:00:00

  • Increased cell-to-cell variation in gene expression in ageing mouse heart.

    abstract::The accumulation of somatic DNA damage has been implicated as a cause of ageing in metazoa. One possible mechanism by which increased DNA damage could lead to cellular degeneration and death is by stochastic deregulation of gene expression. Here we directly test for increased transcriptional noise in aged tissue by di...


    pub_type: 杂志文章


    authors: Bahar R,Hartmann CH,Rodriguez KA,Denny AD,Busuttil RA,Dollé ME,Calder RB,Chisholm GB,Pollock BH,Klein CA,Vijg J

    更新日期:2006-06-22 00:00:00

  • Guanine nucleotide-binding and autophosphorylating activities associated with the p21src protein of Harvey murine sarcoma virus.

    abstract::The purified p21src protein of Harvey sarcoma virus shows a guanine nucleotide-binding activity and, in addition, at elevated temperature an autophosphorylating activity at a threonine residue using as phosphoryl donor GTP or dGTP but not ATP or dATP. These biochemical activities are unique among those associated with...


    pub_type: 杂志文章


    authors: Shih TY,Papageorge AG,Stokes PE,Weeks MO,Scolnick EM

    更新日期:1980-10-23 00:00:00

  • IL-21 and TGF-beta are required for differentiation of human T(H)17 cells.

    abstract::The recent discovery of CD4(+) T cells characterized by secretion of interleukin (IL)-17 (T(H)17 cells) and the naturally occurring regulatory FOXP3(+) CD4 T cell (nT(reg)) has had a major impact on our understanding of immune processes not readily explained by the T(H)1/T(H)2 paradigm. T(H)17 and nT(reg) cells have b...


    pub_type: 杂志文章


    authors: Yang L,Anderson DE,Baecher-Allan C,Hastings WD,Bettelli E,Oukka M,Kuchroo VK,Hafler DA

    更新日期:2008-07-17 00:00:00

  • Molecular dissection of the secretory pathway.

    abstract::A combination of biochemistry in animal cell-free systems and genetics in yeast is revealing the molecular machinery of the secretory pathway of eukaryotes. Transporting vesicles have a simple coat structure and employ a general mechanism for fusion that is conserved in evolution. ...


    pub_type: 杂志文章,评审


    authors: Rothman JE,Orci L

    更新日期:1992-01-30 00:00:00

  • A point mutation in the A gamma-globin gene promoter in Greek hereditary persistence of fetal haemoglobin.

    abstract::Hereditary persistance of fetal haemoglobin (HPFH) is a benign condition characterized by the production in adulthood of more than 1% fetal haemoglobin (HbF, alpha 2 gamma 2) in the absence of erythropoietic stress. Several genetic types have been discerned based on the level of HbF produced, the relative contribution...


    pub_type: 杂志文章


    authors: Collins FS,Metherall JE,Yamakawa M,Pan J,Weissman SM,Forget BG

    更新日期:1985-01-24 00:00:00

  • Publisher Correction: Magnetoelectric inversion of domain patterns.

    abstract::Four incorrect figure citations in this Letter have been corrected online. ...


    pub_type: 杂志文章,已发布勘误


    authors: Leo N,Carolus V,White JS,Kenzelmann M,Hudl M,Tolédano P,Honda T,Kimura T,Ivanov SA,Weil M,Lottermoser T,Meier D,Fiebig M

    更新日期:2018-11-01 00:00:00