Identification of kinesin in sea urchin eggs, and evidence for its localization in the mitotic spindle.

Abstract:

:To understand the molecular basis of microtubule-associated motility during mitosis, the mechanochemical factors that generate the relevant motile force must be identified. Myosin, the ATPase that interacts with actin to produce the force for muscle contraction and other forms of cell motility, is believed to be involved in cytokinesis but not in mitosis. Dynein, the mechanochemical enzyme that drives microtubule sliding in eukaryotic cilia and flagella, has been identified in the cytoplasm of sea urchin eggs, but the evidence that it is involved in cytoplasmic microtubule-based motility (rather than serving as a precursor for embryonic cilia) is equivocal. Microtubule-associated ATPases have been prepared from other tissues, but their role in cytoplasmic motility is also unknown. Recent work on axoplasmic transport, however, has led to the identification of a novel mechanochemical protein called kinesin, which is thought to generate the force for moving vesicles along axonal microtubules. These results suggest that kinesin may also be a mechanochemical factor for non-axoplasmic forms of microtubule-based motility, such as mitosis. We describe here the identification and isolation of a kinesin-like protein from the cytoplasm of sea urchin eggs. We present evidence that this protein is localized in the mitotic spindle, and propose that it may be a mechanochemical factor for some form of motility associated with the mitotic spindle.

journal_name

Nature

journal_title

Nature

authors

Scholey JM,Porter ME,Grissom PM,McIntosh JR

doi

10.1038/318483a0

subject

Has Abstract

pub_date

1985-12-05 00:00:00

pages

483-6

issue

6045

eissn

0028-0836

issn

1476-4687

journal_volume

318

pub_type

杂志文章

相关文献

NATURE文献大全
  • Mechanism of inhibition of the human matrix metalloproteinase stromelysin-1 by TIMP-1.

    abstract::Matrix metalloproteinases (MMPs) are zinc endopeptidases that are required for the degradation of extracellular matrix components during normal embryo development, morphogenesis and tissue remodelling. Their proteolytic activities are precisely regulated by endogenous tissue inhibitors of metalloproteinases (TIMPs). D...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/37995

    authors: Gomis-Rüth FX,Maskos K,Betz M,Bergner A,Huber R,Suzuki K,Yoshida N,Nagase H,Brew K,Bourenkov GP,Bartunik H,Bode W

    更新日期:1997-09-04 00:00:00

  • Sharply increased mass loss from glaciers and ice caps in the Canadian Arctic Archipelago.

    abstract::Mountain glaciers and ice caps are contributing significantly to present rates of sea level rise and will continue to do so over the next century and beyond. The Canadian Arctic Archipelago, located off the northwestern shore of Greenland, contains one-third of the global volume of land ice outside the ice sheets, but...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature10089

    authors: Gardner AS,Moholdt G,Wouters B,Wolken GJ,Burgess DO,Sharp MJ,Cogley JG,Braun C,Labine C

    更新日期:2011-05-19 00:00:00

  • Primary structure of the precursor to the three major surface antigens of Plasmodium falciparum merozoites.

    abstract::Recently, a class of protein antigens of high relative molecular mass (Mt) which can induce protective immunity against blood-stage malaria has been identified. In Plasmodium falciparum the protein has a Mr of approximately 195,000 (P195). It is the precursor of three proteins of Mr 83,000 (83K), 42K and 19K which are...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/317270a0

    authors: Holder AA,Lockyer MJ,Odink KG,Sandhu JS,Riveros-Moreno V,Nicholls SC,Hillman Y,Davey LS,Tizard ML,Schwarz RT

    更新日期:1985-09-19 00:00:00

  • Potassium channel receptor site for the inactivation gate and quaternary amine inhibitors.

    abstract::Many voltage-dependent K+ channels open when the membrane is depolarized and then rapidly close by a process called inactivation. Neurons use inactivating K+ channels to modulate their firing frequency. In Shaker-type K+ channels, the inactivation gate, which is responsible for the closing of the channel, is formed by...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/35079500

    authors: Zhou M,Morais-Cabral JH,Mann S,MacKinnon R

    更新日期:2001-06-07 00:00:00

  • Collagen-mineral axial relationship in calcified turkey leg tendon by X-ray and neutron diffraction.

    abstract::The axial relationship between the collagen and mineral componetns in calcified turkey leg tendon was investigated using low-angle X-ray and neutron diffraction. The results indicate that the mineral is arranged with the same axial periodicity as the collagen and occupies the gap region of the collagen structure. ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/266421a0

    authors: White SW,Hulmes DJ,Miller A,Timmins PA

    更新日期:1977-03-31 00:00:00

  • Accessory cell-dependent selection of specific T-cell functions.

    abstract::Activation of many T-cell functions depends on their interaction with antigen-presenting accessory cells which express I region associated (Ia) products. Cells expressing accessory cell function include those of the monocyte/macrophage lineage and dendritic cells. More recently, B cells and a number of tumour cell lin...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/304442a0

    authors: Ramila G,Erb P

    更新日期:1983-08-04 00:00:00

  • A glial progenitor cell that develops in vitro into an astrocyte or an oligodendrocyte depending on culture medium.

    abstract::We have identified a cell type in 7-day-old rat optic nerve that differentiates into a fibrous astrocyte if cultured in the presence of fetal calf serum and into an oligodendrocyte if cultured in the absence of serum. In certain culture conditions some of these cells acquire a mixed phenotype, displaying properties of...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/303390a0

    authors: Raff MC,Miller RH,Noble M

    更新日期:1983-06-02 00:00:00

  • Lower Palaeolithic hunting spears from Germany.

    abstract::Little is known about the organic component of Lower and Middle Palaeolithic technologies, particular with respect to wooden tools. Here I describe some wooden throwing spears about 400,000 years old that were discovered in 1995 at the Pleistocene site at Schöningen, Germany. They are thought to be the oldest complete...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/385807a0

    authors: Thieme H

    更新日期:1997-02-27 00:00:00

  • Binding of the Drosophila sex-lethal gene product to the alternative splice site of transformer primary transcript.

    abstract::Somatic sexual differentiation in Drosophila melanogaster is accomplished by a hierarchy of genes of which one, Sex-lethal (Sxl), is required for the functional female-specific splicing of the transcripts of the immediately downstream regulatory gene, transformer (tra). The first exon of the tra primary transcript is ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/344461a0

    authors: Inoue K,Hoshijima K,Sakamoto H,Shimura Y

    更新日期:1990-03-29 00:00:00

  • S-phase feedback control in budding yeast independent of tyrosine phosphorylation of p34cdc28.

    abstract::In somatic cells, entry into mitosis depends on the completion of DNA synthesis. This dependency is established by S-phase feedback controls that arrest cell division when damaged or unreplicated DNA is present. In the fission yeast Schizosaccharomyces pombe, mutations that interfere with the phosphorylation of tyrosi...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/355365a0

    authors: Sorger PK,Murray AW

    更新日期:1992-01-23 00:00:00

  • The adult Drosophila posterior midgut is maintained by pluripotent stem cells.

    abstract::Vertebrate and invertebrate digestive systems show extensive similarities in their development, cellular makeup and genetic control. The Drosophila midgut is typical: enterocytes make up the majority of the intestinal epithelial monolayer, but are interspersed with hormone-producing enteroendocrine cells. Human (and m...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature04333

    authors: Ohlstein B,Spradling A

    更新日期:2006-01-26 00:00:00

  • Controlled vesicle deformation and lysis by single oscillating bubbles.

    abstract::The ability of collapsing (cavitating) bubbles to focus and concentrate energy, forces and stresses is at the root of phenomena such as cavitation damage, sonochemistry or sonoluminescence. In a biomedical context, ultrasound-driven microbubbles have been used to enhance contrast in ultrasonic images. The observation ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature01613

    authors: Marmottant P,Hilgenfeldt S

    更新日期:2003-05-08 00:00:00

  • Changes of mind in decision-making.

    abstract::A decision is a commitment to a proposition or plan of action based on evidence and the expected costs and benefits associated with the outcome. Progress in a variety of fields has led to a quantitative understanding of the mechanisms that evaluate evidence and reach a decision. Several formalisms propose that a repre...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature08275

    authors: Resulaj A,Kiani R,Wolpert DM,Shadlen MN

    更新日期:2009-09-10 00:00:00

  • Bidirectional perisomatic inhibitory plasticity of a Fos neuronal network.

    abstract::Behavioural experiences activate the FOS transcription factor in sparse populations of neurons that are critical for encoding and recalling specific events1-3. However, there is limited understanding of the mechanisms by which experience drives circuit reorganization to establish a network of Fos-activated cells. It i...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/s41586-020-3031-0

    authors: Yap EL,Pettit NL,Davis CP,Nagy MA,Harmin DA,Golden E,Dagliyan O,Lin C,Rudolph S,Sharma N,Griffith EC,Harvey CD,Greenberg ME

    更新日期:2020-12-09 00:00:00

  • Global environmental consequences of twenty-first-century ice-sheet melt.

    abstract::Government policies currently commit us to surface warming of three to four degrees Celsius above pre-industrial levels by 2100, which will lead to enhanced ice-sheet melt. Ice-sheet discharge was not explicitly included in Coupled Model Intercomparison Project phase 5, so effects on climate from this melt are not cur...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/s41586-019-0889-9

    authors: Golledge NR,Keller ED,Gomez N,Naughten KA,Bernales J,Trusel LD,Edwards TL

    更新日期:2019-02-01 00:00:00

  • RIM1alpha is required for presynaptic long-term potentiation.

    abstract::Two main forms of long-term potentiation (LTP)-a prominent model for the cellular mechanism of learning and memory-have been distinguished in the mammalian brain. One requires activation of postsynaptic NMDA (N-methyl d-aspartate) receptors, whereas the other, called mossy fibre LTP, has a principal presynaptic compon...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/415327a

    authors: Castillo PE,Schoch S,Schmitz F,Südhof TC,Malenka RC

    更新日期:2002-01-17 00:00:00

  • Ban on triazine herbicides likely to reduce but not negate relative benefits of GMHT maize cropping.

    abstract::The UK Farm-Scale Evaluations (FSE) compared the effects on biodiversity of management of genetically modified herbicide-tolerant (GMHT) spring-sown crops with conventional crop management. The FSE reported larger weed abundance under GMHT management for fodder maize, one of three crops studied. Increased seed product...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature02374

    authors: Perry JN,Firbank LG,Champion GT,Clark SJ,Heard MS,May MJ,Hawes C,Squire GR,Rothery P,Woiwod IP,Pidgeon JD

    更新日期:2004-03-18 00:00:00

  • Integrin-YAP/TAZ-JNK cascade mediates atheroprotective effect of unidirectional shear flow.

    abstract::The Yorkie homologues YAP (Yes-associated protein) and TAZ (transcriptional coactivator with PDZ-binding motif, also known as WWTR1), effectors of the Hippo pathway, have been identified as mediators for mechanical stimuli. However, the role of YAP/TAZ in haemodynamics-induced mechanotransduction and pathogenesis of a...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature20602

    authors: Wang L,Luo JY,Li B,Tian XY,Chen LJ,Huang Y,Liu J,Deng D,Lau CW,Wan S,Ai D,Mak KK,Tong KK,Kwan KM,Wang N,Chiu JJ,Zhu Y,Huang Y

    更新日期:2016-12-22 00:00:00

  • Deep-sea ecology. Developmental arrest in vent worm embryos.

    abstract::Temperature is a key factor in controlling the distribution of marine organisms and is particularly important at hydrothermal vents, where steep thermal gradients are present over a scale of centimetres. The thermophilic worm Alvinella pompejana, which is found at the vents of the East Pacific Rise (2,500-m depth), ha...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/35099674

    authors: Pradillon F,Shillito B,Young CM,Gaill F

    更新日期:2001-10-18 00:00:00

  • Expression of the c-fms proto-oncogene during human monocytic differentiation.

    abstract::The McDonough strain of the feline sarcoma virus contains a transforming gene (v-fms) which contains partial nucleotide homology with proto-oncogenes encoding tyrosine kinases. One of the v-fms-encoded products, gp140fms, is a cell surface transmembrane glycoprotein that may function as a growth factor receptor. Altho...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/316064a0

    authors: Sariban E,Mitchell T,Kufe D

    更新日期:1985-07-04 00:00:00

  • Computational design of ligand-binding proteins with high affinity and selectivity.

    abstract::The ability to design proteins with high affinity and selectivity for any given small molecule is a rigorous test of our understanding of the physiochemical principles that govern molecular recognition. Attempts to rationally design ligand-binding proteins have met with little success, however, and the computational d...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature12443

    authors: Tinberg CE,Khare SD,Dou J,Doyle L,Nelson JW,Schena A,Jankowski W,Kalodimos CG,Johnsson K,Stoddard BL,Baker D

    更新日期:2013-09-12 00:00:00

  • Novel neurotrophic factor CDNF protects and rescues midbrain dopamine neurons in vivo.

    abstract::In Parkinson's disease, brain dopamine neurons degenerate most prominently in the substantia nigra. Neurotrophic factors promote survival, differentiation and maintenance of neurons in developing and adult vertebrate nervous system. The most potent neurotrophic factor for dopamine neurons described so far is the glial...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature05957

    authors: Lindholm P,Voutilainen MH,Laurén J,Peränen J,Leppänen VM,Andressoo JO,Lindahl M,Janhunen S,Kalkkinen N,Timmusk T,Tuominen RK,Saarma M

    更新日期:2007-07-05 00:00:00

  • Immune clearance of highly pathogenic SIV infection.

    abstract::Established infections with the human and simian immunodeficiency viruses (HIV and SIV, respectively) are thought to be permanent with even the most effective immune responses and antiretroviral therapies only able to control, but not clear, these infections. Whether the residual virus that maintains these infections ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature12519

    authors: Hansen SG,Piatak M Jr,Ventura AB,Hughes CM,Gilbride RM,Ford JC,Oswald K,Shoemaker R,Li Y,Lewis MS,Gilliam AN,Xu G,Whizin N,Burwitz BJ,Planer SL,Turner JM,Legasse AW,Axthelm MK,Nelson JA,Früh K,Sacha JB,Estes JD

    更新日期:2013-10-03 00:00:00

  • Gasdermin E suppresses tumour growth by activating anti-tumour immunity.

    abstract::Cleavage of the gasdermin proteins to produce pore-forming amino-terminal fragments causes inflammatory cell death (pyroptosis)1. Gasdermin E (GSDME, also known as DFNA5)-mutated in familial ageing-related hearing loss2-can be cleaved by caspase 3, thereby converting noninflammatory apoptosis to pyroptosis in GSDME-ex...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/s41586-020-2071-9

    authors: Zhang Z,Zhang Y,Xia S,Kong Q,Li S,Liu X,Junqueira C,Meza-Sosa KF,Mok TMY,Ansara J,Sengupta S,Yao Y,Wu H,Lieberman J

    更新日期:2020-03-01 00:00:00

  • Synergism between immunoglobulin enhancers and promoters.

    abstract::Enhancers are DNA sequences that stimulate transcription from eukaryotic promoters. This stimulatory effect can be exerted over large distances and from a position either 5' or 3' of a promoter. Enhancers have been found in the genomes of many viruses, and in some cellular genes such as those encoding immunoglobulin h...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/322383a0

    authors: Garcia JV,Bich-Thuy LT,Stafford J,Queen C

    更新日期:1986-07-24 00:00:00

  • Building a better lymphoma vaccine.

    abstract:: ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/d41586-018-07366-1

    authors: Ainsworth C

    更新日期:2018-11-01 00:00:00

  • Clonal selection drives genetic divergence of metastatic medulloblastoma.

    abstract::Medulloblastoma, the most common malignant paediatric brain tumour, arises in the cerebellum and disseminates through the cerebrospinal fluid in the leptomeningeal space to coat the brain and spinal cord. Dissemination, a marker of poor prognosis, is found in up to 40% of children at diagnosis and in most children at ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature10825

    authors: Wu X,Northcott PA,Dubuc A,Dupuy AJ,Shih DJ,Witt H,Croul S,Bouffet E,Fults DW,Eberhart CG,Garzia L,Van Meter T,Zagzag D,Jabado N,Schwartzentruber J,Majewski J,Scheetz TE,Pfister SM,Korshunov A,Li XN,Scherer SW,Ch

    更新日期:2012-02-15 00:00:00

  • Bell violation using entangled photons without the fair-sampling assumption.

    abstract::The violation of a Bell inequality is an experimental observation that forces the abandonment of a local realistic viewpoint--namely, one in which physical properties are (probabilistically) defined before and independently of measurement, and in which no physical influence can propagate faster than the speed of light...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature12012

    authors: Giustina M,Mech A,Ramelow S,Wittmann B,Kofler J,Beyer J,Lita A,Calkins B,Gerrits T,Nam SW,Ursin R,Zeilinger A

    更新日期:2013-05-09 00:00:00

  • Spectroscopy of short-lived radioactive molecules.

    abstract::Molecular spectroscopy offers opportunities for the exploration of the fundamental laws of nature and the search for new particle physics beyond the standard model1-4. Radioactive molecules-in which one or more of the atoms possesses a radioactive nucleus-can contain heavy and deformed nuclei, offering high sensitivit...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/s41586-020-2299-4

    authors: Garcia Ruiz RF,Berger R,Billowes J,Binnersley CL,Bissell ML,Breier AA,Brinson AJ,Chrysalidis K,Cocolios TE,Cooper BS,Flanagan KT,Giesen TF,de Groote RP,Franchoo S,Gustafsson FP,Isaev TA,Koszorús Á,Neyens G,Perrett HA

    更新日期:2020-05-01 00:00:00

  • Pressure sensitivity of olivine slip systems and seismic anisotropy of Earth's upper mantle.

    abstract::The mineral olivine dominates the composition of the Earth's upper mantle and hence controls its mechanical behaviour and seismic anisotropy. Experiments at high temperature and moderate pressure, and extensive data on naturally deformed mantle rocks, have led to the conclusion that olivine at upper-mantle conditions ...

    journal_title:Nature

    pub_type: 杂志文章

    doi:10.1038/nature03266

    authors: Mainprice D,Tommasi A,Couvy H,Cordier P,Frost DJ

    更新日期:2005-02-17 00:00:00