REV1 mediated mutagenesis in base excision repair deficient mouse fibroblast.

abstract：:We have identified two fission yeast homologs of budding yeast Rad4 and human xeroderma pigmentosum complementation group C (XP-C) correcting protein, designated Rhp4A and Rhp4B. Here we show that the rhp4 genes encode NER factors that are required for UV-induced DNA damage repair in fission yeast. The rhp4A-deficient...

journal_title：DNA repair

authors： Fukumoto Y,Hiyama H,Yokoi M,Nakaseko Y,Yanagida M,Hanaoka F

更新日期：2002-10-01 00:00:00

abstract：:The mammalian CtIP protein and its orthologs in other eukaryotes promote the resection of DNA double-strand breaks and are essential for meiotic recombination. Here we review the current literature supporting the role of CtIP in DNA end processing and the importance of CtIP endonuclease activity in DNA repair. We also...

journal_title：DNA repair

authors： Makharashvili N,Paull TT

更新日期：2015-08-01 00:00:00

abstract：:Methylation of the O(6)-methylguanine-DNA-methyltransferase (MGMT) promoter is associated with G:C to A:T transitions in the p53 gene in various human cancers, including lung cancer. In tumors with p53 mutation, MGMT promoter methylation is more common in advanced tumors than in early tumors. However, in tumors with w...

journal_title：DNA repair

authors： Lai JC,Cheng YW,Goan YG,Chang JT,Wu TC,Chen CY,Lee H

更新日期：2008-08-02 00:00:00

abstract：:Oxidative DNA damage is repaired primarily by the base excision repair (BER) pathway in a process initiated by removal of base lesions or mismatched bases by DNA glycosylases. MutY homolog (MYH, MUTYH, or Myh1) is a DNA glycosylase which excises adenine paired with the oxidative lesion 8-oxo-7,8-dihydroguanine (8-oxoG...

journal_title：DNA repair

authors： Jin J,Hwang BJ,Chang PW,Toth EA,Lu AL

更新日期：2014-03-01 00:00:00

abstract：:The RAD6/RAD18 heterodimer promotes translesion synthesis via the monoubiquitination of the DNA sliding clamp, PCNA. In S. cerevisiae, a second complex, UBC13/MMS2/RAD5, can extend this single ubiquitin with a non-canonical lysine 63-linked chain. This polyubiquitination step is required for an error-free mode of bypa...

journal_title：DNA repair

authors： Simpson LJ,Sale JE

更新日期：2005-04-04 00:00:00

abstract：:Chemicals used industrially and commercially are required by law to be assessed for their genotoxic potential. However, all currently used assays have major limitations and despite intense effort, there is no universal agreement on which tests should be employed, or how to interpret results. We have developed a new as...

journal_title：DNA repair

authors： Evans TJ,Yamamoto KN,Hirota K,Takeda S

更新日期：2010-12-10 00:00:00

abstract：:DNA repair is often a complex, multi-component, multi-step process; this makes detailed kinetic analysis of the different steps of repair a challenging task using standard biochemical methods. At the same time, single-molecule methods are well-suited for extracting kinetic information despite time-averaging due to dif...

journal_title：DNA repair

authors： Howan K,Monnet J,Fan J,Strick TR

更新日期：2014-08-01 00:00:00

abstract：:Like many other cellular processes, regulation of the DNA damage response (DDR) is regulated at different levels, ranging from transcriptional control to an array of distinct post-translational modifications. Involvement of ubiquitylation and the ubiquitin proteasome system in adjusting DDR are such protein modificati...

journal_title：DNA repair

authors： Bergink S,Jaspers NG,Vermeulen W

更新日期：2007-09-01 00:00:00

abstract：:We have used the recently determined crystal structures of Escherichia coli (E. coli) MutS, MutL and MutH to guide construction of 47 amino-acid substitutions in these proteins and analyzed their behavior in mismatch repair and recombination in vitro and in vivo. We find that the active site of the MutH endonuclease i...

journal_title：DNA repair

authors： Junop MS,Yang W,Funchain P,Clendenin W,Miller JH

更新日期：2003-04-02 00:00:00

abstract：:Maintenance of DNA integrity is vital for all of the living organisms. Consequence of DNA damaging ranges from, introducing harmless synonymous mutations, to causing disease-associated mutations, genome instability, and cell death. A cell cycle protein cyclin D1 is an established cancer-driving protein. However, contr...

journal_title：DNA repair

authors： Jirawatnotai S,Sittithumcharee G

更新日期：2016-06-01 00:00:00

abstract：:Human APOBEC3G (A3G) and activation-induced deaminase (AID) belong to a family of DNA-cytosine deaminases. While A3G targets the last C in a run of C's, AID targets C in the consensus sequence WRC (W is A or T and R is a purine). Guided by the structures of the A3G carboxyl-terminal catalytic domain (A3G-CTD), we iden...

journal_title：DNA repair

authors： Carpenter MA,Rajagurubandara E,Wijesinghe P,Bhagwat AS

更新日期：2010-05-04 00:00:00

abstract：:Eukaryotic genomes are duplicated by a complex machinery, utilizing high fidelity replicative B-family DNA polymerases (pols) α, δ and ε. Specialized error-prone pol ζ, the fourth B-family member, is recruited when DNA synthesis by the accurate trio is impeded by replication stress or DNA damage. The damage tolerance ...

journal_title：DNA repair

authors： Stepchenkova EI,Tarakhovskaya ER,Siebler HM,Pavlov YI

更新日期：2017-01-01 00:00:00

abstract：:Quantitating relative (32)P-band intensity in gels is desired, e.g., to study primer-extension kinetics of DNA polymerases (DNAPs). Following imaging, multiple (32)P-bands are often present in lanes. Though individual bands appear by eye to be simple and well-resolved, scanning reveals they are actually skewed-Gaussia...

journal_title：DNA repair

authors： Sholder G,Loechler EL

更新日期：2015-01-01 00:00:00

abstract：:DNA interstrand crosslinks (ICLs) are highly toxic lesions that covalently link both strands of DNA and distort the DNA helix. Crosslinking agents have been shown to stall DNA replication and failure to repair ICL lesions before encountered by replication forks may induce severe DNA damage. Most knowledge of the ICL r...

journal_title：DNA repair

authors： Vare D,Groth P,Carlsson R,Johansson F,Erixon K,Jenssen D

更新日期：2012-12-01 00:00:00

abstract：:Reactive oxygen species drive the oxidation of guanine to 8-oxoguanine (8oxoG), which threatens genome integrity. The repair of 8oxoG is carried out by base excision repair enzymes in Bacteria and Eukarya, however, little is known about archaeal 8oxoG repair. This study identifies a member of the Ogg-subfamily archaea...

journal_title：DNA repair

authors： Gehring AM,Zatopek KM,Burkhart BW,Potapov V,Santangelo TJ,Gardner AF

更新日期：2020-02-01 00:00:00

abstract：:The DNA damage response is a key factor in the maintenance of genome stability. As such, it is a central axis in sustaining cellular homeostasis in a variety of contexts: development, growth, differentiation, and maintenance of the normal life cycle of the cell. It is now clear that diverse mechanisms encompassing cel...

journal_title：DNA repair

authors： Barzilai A,Biton S,Shiloh Y

更新日期：2008-07-01 00:00:00

abstract：:In both Schizosaccharomyces pombe and Saccharomyces cerevisiae, Mms22 and Mms1 form a complex with important functions in the response to DNA damage, loss of which leads to perturbations during replication. Furthermore, in S. cerevisiae, Mms1 has been suggested to function in concert with a Cullin-like protein, Rtt101...

journal_title：DNA repair

authors： Vejrup-Hansen R,Mizuno K,Miyabe I,Fleck O,Holmberg C,Murray JM,Carr AM,Nielsen O

更新日期：2011-03-07 00:00:00

abstract：:Mitochondria are membrane-bound organelles found in eukaryotic cells where they generate energy through the respiratory chain. They contain their own genome that encodes genes critical to the mitochondrial function, but most of their protein content is synthetized from nuclear encoded genes. Damages to the mtDNA can c...

journal_title：DNA repair

authors： Bernal M,Yang X,Lisby M,Mazón G

更新日期：2019-10-01 00:00:00

abstract：:Non-B DNA conformations adopted by certain types of DNA sequences promote genetic instabilities, especially gross rearrangements including translocations. We conclude the following: (a) slipped (hairpin) structures, cruciforms, triplexes, tetraplexes and i-motifs, and left-handed Z-DNA are formed in chromosomes and el...

journal_title：DNA repair

authors： Bacolla A,Wojciechowska M,Kosmider B,Larson JE,Wells RD

更新日期：2006-09-08 00:00:00

abstract：:RECQ proteins are conserved DNA helicases in both prokaryotes and eukaryotes. The importance of the RECQ family helicases in human health is demonstrated by their roles as cancer suppressors that are vital for preserving genome integrity. Mutations in one of the RECQ family proteins, RECQ4, not only result in developm...

journal_title：DNA repair

authors： Liu Y

更新日期：2010-03-02 00:00:00

abstract：:The continuity of duplex DNA is generally considered a prerequisite for chromosome continuity. However, as previously shown in yeast as well as human cells, the introduction of a double-strand break (DSB) does not generate a chromosome break (CRB) in yeast or human cells. The transition from DSB to CRB was found to be...

journal_title：DNA repair

authors： Nakai W,Westmoreland J,Yeh E,Bloom K,Resnick MA

更新日期：2011-01-02 00:00:00

abstract：:Single-strand-dependent DNA exonucleases play important roles in DNA repair and recombination in all organisms. In Escherichia coli the redundant functions provided by the RecJ, ExoI, ExoVII and ExoX exonucleases are required for mismatch repair, UV resistance and homologous recombination. We have examined whether the...

journal_title：DNA repair

authors： Lombardo MJ,Aponyi I,Ray MP,Sandigursky M,Franklin WA,Rosenberg SM

更新日期：2003-11-21 00:00:00

abstract：:Gene amplification, a key mechanism for oncogene activation and drug resistance in tumour cells, involves the generation and joining of DNA double-strand breaks. Amplified DNA can be carried either on intra-chromosomal arrays or on extra-chromosomal elements (double minutes). We previously showed that, in rodent cells...

journal_title：DNA repair

authors： Salzano A,Kochiashvili N,Nergadze SG,Khoriauli L,Smirnova A,Ruiz-Herrera A,Mondello C,Giulotto E

更新日期：2009-01-01 00:00:00

abstract：:During DNA synthesis in vitro using dNTP and rNTP concentrations present in vivo, yeast replicative DNA polymerases α, δ and ɛ (Pols α, δ and ɛ) stably incorporate rNTPs into DNA. rNTPs are also incorporated during replication in vivo, and they are repaired in an RNase H2-dependent manner. In strains encoding a mutato...

journal_title：DNA repair

authors： Clark AB,Lujan SA,Kissling GE,Kunkel TA

更新日期：2011-05-05 00:00:00

abstract：:Budding yeast SGS1 and the human Bloom's syndrome (BS) gene, BLM, are homologues of the Escherichia coli recQ. Cells derived from BS patients are characterized by a dramatic increase in sister chromatid exchange (SCE). We previously reported that budding yeast cells deficient in SGS1 showed an increase in the frequenc...

journal_title：DNA repair

authors： Onoda F,Seki M,Wang W,Enomoto T

更新日期：2004-10-05 00:00:00

abstract：:Thymidylate deprivation brings about "thymineless death" in prokaryotes and eukaryotes. Although the precise mechanism for thymineless death has remained elusive, inhibition of the enzyme thymidylate synthase (TS), which catalyzes the de novo synthesis of TMP, has served for many years as a basis for chemotherapeutic ...

journal_title：DNA repair

authors： Waldman BC,Wang Y,Kilaru K,Yang Z,Bhasin A,Wyatt MD,Waldman AS

更新日期：2008-10-01 00:00:00

abstract：:Oxidative stress via redox reactions can regulate DNA repair pathways. The base excision repair (BER) enzyme apurinic/apyrimidinic endonuclease 1 (APE1) is a key player in the redox regulation of DNA repair. Environmental factors can alter the methylation of DNA repair genes, change their expression and thus modulate ...

journal_title：DNA repair

authors： Langie SA,Kowalczyk P,Tomaszewski B,Vasilaki A,Maas LM,Moonen EJ,Palagani A,Godschalk RW,Tudek B,van Schooten FJ,Berghe WV,Zabielski R,Mathers JC

更新日期：2014-06-01 00:00:00

abstract：:The RecQ family of DNA helicases performs essential functions in the maintenance of genomic stability in all organisms. In Deinococcus radiodurans, DR1289 is a special member of RecQ family with unique arrangement of three tandem HRDC domains in the C-terminus. A dr1289 mutant is hypersensitive to gamma-irradiation, U...

journal_title：DNA repair

authors： Huang L,Hua X,Lu H,Gao G,Tian B,Shen B,Hua Y

更新日期：2007-02-04 00:00:00

abstract：:An important feature of poly(ADP-ribose) polymerases (PARPs) is their ability to readily undergo automodification upon activation. Although a growing number of substrates were found to be poly(ADP-ribosyl)ated, including histones and several DNA damage response factors, PARPs themselves are still considered as the mai...

journal_title：DNA repair

authors： Gagné JP,Ethier C,Defoy D,Bourassa S,Langelier MF,Riccio AA,Pascal JM,Moon KM,Foster LJ,Ning Z,Figeys D,Droit A,Poirier GG

更新日期：2015-06-01 00:00:00

abstract：:The MutS2 homologues have been found widespread in most prokaryotes, which are involved in DNA repair and reactive oxygen species detoxification. The C-terminal small mutS-related (Smr) domain is critical for its endonucleolytic activity. However, the detailed catalytic mechanism is still unclear. In this study, we fi...

journal_title：DNA repair

authors： Zhang H,Xu Q,Lu M,Xu X,Wang Y,Wang L,Zhao Y,Hua Y

更新日期：2014-09-01 00:00:00