Abstract：
:The t(8;21)(q22;q22) rearrangement is observed in about 15% of acute myelocitic leukemia (AML) cases, while variant t(8;21) translocations are detected in 6-10% of AML patients positive for the 5'RUNX1/3'CBFA2T1 fusion gene. We report a detailed molecular cytogenetic analysis of a four-way variant t(8;11;16;21)(q22;q14;q12;q22) performed by fluorescence in situ hybridization with specific BAC and PAC clones. The study demonstrated the loss of several megabases belonging to chromosomes 11 and 16 whereas no deletion was detected on der(21). These findings suggest that a precise breakpoint characterization could identify submicroscopic genomic deletions whose meaning remains to be defined.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Albano F,Specchia G,Anelli L,Liso A,Zagaria A,Santoro A,Mirto S,Liso V,Rocchi Mdoi
10.1016/j.leukres.2004.12.018keywords:
subject
Has Abstractpub_date
2005-07-01 00:00:00pages
855-8issue
7eissn
0145-2126issn
1873-5835pii
S0145-2126(05)00044-5journal_volume
29pub_type
杂志文章abstract：:Previous studies have shown that thymosin (fraction 5) is able to induce surface differentiation markers on normal murine bone marrow T-cell precursors. Phorbol ester (TPA) promotes differentiation of human leukaemic lymphoblasts as assessed by changes in phenotypic surface markers and terminal deoxynucleotidyl transf...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(83)90072-3
更新日期：1983-01-01 00:00:00
abstract：:Using the human erythroleukaemic cell line K562 cl.6 and its daunorubicin-resistant subline K/DAU600, and the human T-lymphoblastic leukaemic cell line CCRF-CEM and its vinblastine-resistant subline CEM/VLB100, we have shown that the drug-resistant cell lines were more sensitive to cytotoxicity induced by tumour necro...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(94)00149-5
更新日期：1995-03-01 00:00:00
abstract：:Increased DNA damage and alteration of the DNA damage response (DDR) are critical features of genetic instability presumably implicated in pathogenesis of myelodysplastic syndromes (MDS) and acute myeloid leukemias (AML). We used immunofluorescence staining of γH2AX and 53BP1 for analyzing DNA double-strand breaks (DS...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2017.03.011
更新日期：2017-06-01 00:00:00
abstract：:Acute promyelocytic leukemia (APL) is characterized by a reciprocal translocation, t(15;17) (q22;q12), resulting in fusion of the genes promyelocytic leukemia (PML) and retinoic acid receptor alpha (RARA). With conventional cytogenetic methods, these translocations are detected in about 70-90% of patients, with most o...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2006.05.011
更新日期：2007-02-01 00:00:00
abstract：:In biology, the term "clone" is most widely used to designate genetically identical cells or organisms that are asexually descended from a common progenitor. The concept of clonality in hematology-oncology has received much attention in recent years, as the advent of next-generation sequencing platforms has provided n...
journal_title：Leukemia research
pub_type： 历史文章,杂志文章
doi：10.1016/j.leukres.2017.03.004
更新日期：2017-06-01 00:00:00
abstract：:KN-62, an inhibitor of the calmodulin-dependent protein kinases (CaMKs), enhances the terminal differentiation of retinoic acid sensitive human myeloid leukemia cell lines. In an effort to identify additional CaMK inhibitors that exhibit more potent activity in triggering leukemia cell differentiation, we synthesized ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2006.07.022
更新日期：2007-05-01 00:00:00
abstract：:Increasing evidence suggest that epigenetic mechanisms (e.g. histone modification by histone deacetylases) play a major role in the pathogenesis of Hodgkin's lymphoma (HL). We treated HL cell lines with the histone deacetylase inhibitor vorinostat and investigated the gene expression profile of these cells by using DN...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2012.02.021
更新日期：2012-06-01 00:00:00
abstract：:The CBFA2 gene on chromosome band 21q22 is one of the most commonly translocated genes in leukemia. As with other translocations, those involving CBFA2 are associated with specific disease phenotypes. Only one of the different translocations involving CBFA2, the t(12;21), has been associated with a non-myeloid lineage...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(99)00150-2
更新日期：2000-01-01 00:00:00
abstract：:A bioassay was developed to assess P-glycoprotein (P-gp) function of peripheral blood natural killer (NK) cells and AML blasts during zosuquidar infusion. Cells were incubated with the fluorescent dye DiOC(2)(3) in the presence and absence of zosuquidar, and dye accumulation measured by flow cytometry. The assay perfo...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2008.09.020
更新日期：2009-06-01 00:00:00
abstract：:The studies aimed at identifying a prognostic significance of angiogenesis-related factors: CD105 and placental growth factor (PlGF) in a course of acute lymphoblastic leukaemia (ALL). Research protocol was based on detection of RNA and protein expressions in bone marrow blasts using quantitative PCR and immunocytoche...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2012.03.017
更新日期：2012-07-01 00:00:00
abstract：:Normal human monocytes express Fas and are susceptible to Fas ligand (FasL)-induced apoptosis. Because the myeloid leukemia cell lines HL-60 and THP-1 can be differentiated into functional monocytes and macrophages, we studied their expression of Fas and Fas ligand (FasL) to determine whether there were differentiatio...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(99)00101-0
更新日期：1999-10-01 00:00:00
abstract：:Our aim was to examine in 17 patients with MDS the effects of PMA activated and non-activated autologous lymphocytes on selected bone marrow CD34+ progenitors, in dose response studies. We used a double layer culture technique. Compared with controls, there was no difference in the colony growth promoting capacity of ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2011.03.017
更新日期：2011-09-01 00:00:00
abstract：:Model cell lines are essential tools for investigating the biology and therapeutics of cancer. Approximately 1500 human hematopoietic neoplastic cell lines have been described, covering most major disease entities. Waldenström's macroglobulinemia (WM) is a rare incurable hematological neoplasm from which only three ce...
journal_title：Leukemia research
pub_type： 杂志文章,评审
doi：10.1016/j.leukres.2008.04.016
更新日期：2008-11-01 00:00:00
abstract：BACKGROUND:T- cell prolymphocytic leukemia (T- PLL) is a rare aggressive hematological malignancy. Alemtuzumab, an anti-CD52 humanized monoclonal antibody, is the treatment of choice for remission induction. Allogeneic hematopoietic cell transplantation (allo-HCT) has been described to induce durable remissions and imp...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2018.01.009
更新日期：2018-04-01 00:00:00
abstract：:Proliferative responses of mononuclear cells in liquid cultures to phytohemagglutinin, a T-cell mitogen, are depressed for a long time after allogeneic marrow transplantation. We examined the role of interleukin-2, a lymphokine important in T-cell mitogenesis, in the impaired phytohemagglutinin responses early after m...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(84)90081-x
更新日期：1984-01-01 00:00:00
abstract：:Osteogenic growth peptide (OGP) is a peptide exerting regulatory effects on the bone and on bone marrow. The carboxy-terminal pentapeptide (OGP10-14) is the biologically active portion of OGP. We evaluated OGP10-14 hematopoietic activity performing colony-forming tests on human stem cells derived by bone marrow, perip...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(02)00008-5
更新日期：2002-09-01 00:00:00
abstract：:We wish to demonstrate new evidence for the in vivo production of interleukin-3 (IL-3). Syngeneic murine splenocytes were transplanted into lethally irradiated mice. The spleen cells in these transplant mice spontaneously produced IL-3 in cultures, and IL-3-like activity was detected in the serum. Chronological change...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(89)90138-0
更新日期：1989-01-01 00:00:00
abstract：:Aplastic anemia, myelodysplastic syndromes (MDS) and chronic myeloproliferative diseases (MPD) are stem cell disorders. There is no clear-cut demarcation of them. Hypoplastic MDS displays features of aplastic anemia and MDS, on the other side mixed myelodysplastic and myeloproliferative syndromes (MDS-MPS) develop. In...
journal_title：Leukemia research
pub_type： 杂志文章,多中心研究
doi：10.1016/0145-2126(96)00028-8
更新日期：1996-09-01 00:00:00
abstract：:The human cell line U-937 has many features suggestive of the monocyte. It may be stimulated in vitro to develop attributes of an activated macrophage with increased phagocytosis and enhanced Fc receptor expression. We describe the establishment of a subclone designated U-937 AG through the use of a mutagenic agent. T...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(84)90084-5
更新日期：1984-01-01 00:00:00
abstract：:We have utilized the MTT assay to measure the metabolic activity of cells from the bone marrow of 55 patients with acute myeloid leukaemia (AML), myelodysplastic syndrome (MDS) and non-clonal disease. Doubling dilutions of cells were exposed to MTT for 3-4 h. The mean optical density of the formazan produced by each c...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(95)00163-8
更新日期：1996-05-01 00:00:00
abstract：:The tumor microenvironment (TME) is critical to the longevity of tumor B cells in chronic lymphocytic leukemia (CLL). Bone marrow mesenchymal stem cells (BMMSCs) and the cytokines they produce including IL-6 are important components of the TME in CLL. We found BMMSCs supported the survival of CLL cells in vitro throug...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2018.01.006
更新日期：2018-03-01 00:00:00
abstract：:A synthetic pentadecapeptide preparation, env 406-420, with an amino acid sequence deduced from the envelope glycoprotein gene of human T cell leukemia virus type I (HTLV I), was used as the antigen in an enzyme immunoassay for immunoglobulin G antibodies, exploring its usefulness for seroepidemiological purposes. The...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(85)90100-6
更新日期：1985-01-01 00:00:00
abstract：:1,25-Dihydroxyvitamin D3 (1,25D) induces differentiation of myeloid leukemia cells, but resistant cells are also encountered. We studied the mechanistic basis for the resistance in a model system using enhancers of 1,25D, the antioxidant carnosic acid and a kinase inhibitor SB202190. Knock-down (KD) of JNK2p54 unexpec...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2009.03.003
更新日期：2009-10-01 00:00:00
abstract：:DNA aneuploidy has been used as a genetic marker of malignancy in multiple myeloma (MM). CD38 and CD138 expression and absence of CD22 and CD19 may define plasmacells (PC). Several authors support evidences of circulating plasmacells, and their role in relapse after autologous stem cell transplantation has been hypoth...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2003.09.015
更新日期：2004-05-01 00:00:00
abstract：:The purpose of this study was to determine the sensitivity to merocyanine 540 (MC 540)-mediated photolysis of normal human hematopoietic progenitor cells and four leukemia cell lines (Daudi, Raji, K562 and HL-60). Late erythroid progenitors were the most sensitive normal cells. Early erythroid progenitors were of inte...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(87)90104-4
更新日期：1987-01-01 00:00:00
abstract：:We determined the position of the breakpoint within the bcr gene in 22 patients with Philadelphia-positive chronic myelocytic leukemia using conventional Southern-blots and analyzed its relationship to thrombopoiesis. After DNA digestion with restriction endonucleases (Hind III, Bam HI and Bgl II), we localized the br...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(91)90113-8
更新日期：1991-01-01 00:00:00
abstract：:We evaluated outcomes in two consecutive groups of AML patients age>60 years in CR after 7+3 induction therapy. Group 1 received consolidation with cytarabine 1.5g/m(2) q12h×6+daunorubicin for two cycles, while group 2 received consolidation with 7+3 followed by mitoxantrone+etoposide. For patients with intermediate-r...
journal_title：Leukemia research
pub_type： 临床试验,杂志文章
doi：10.1016/j.leukres.2013.01.001
更新日期：2013-05-01 00:00:00
abstract：:Heterogeneity for 5'nucleotidase has been demonstrated in chronic lymphocytic leukemia [12]. The enzyme is not detectable in the lymphocytes from the majority of patients with this disorder, but normal and even supranormal levels are found in some cases [17]. In the present studies, the properties of this ecto-nucleot...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(82)90026-1
更新日期：1982-01-01 00:00:00
abstract：:In this report we evaluated the exact expression pattern of c-Kit on mobilized peripheral blood (PB) CD34+ cells. Using a monoclonal antibody against CD117 antigen (95C3), flow cytometric analysis revealed that approximately 25% of the mobilized PB CD34+ cells coexpress c-Kit. This cell fraction showed a considerable ...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/s0145-2126(98)00002-2
更新日期：1998-04-01 00:00:00
abstract：BACKGROUND:Myelodysplastic syndromes (MDS) are a heterogeneous group of clonal disorders, with very different prognosis in given individuals; age and comorbidities are emerging as relevant patient-related factors influencing clinical outcome in MDS. Our aim was to evaluate the impact of age, comorbidities and disease s...
journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2015.05.007
更新日期：2015-08-01 00:00:00
