Abstract：
:We determined the position of the breakpoint within the bcr gene in 22 patients with Philadelphia-positive chronic myelocytic leukemia using conventional Southern-blots and analyzed its relationship to thrombopoiesis. After DNA digestion with restriction endonucleases (Hind III, Bam HI and Bgl II), we localized the breakpoint in bcr using two genomic probes. The location of the breakpoint within the bcr was assigned to one of five zones. Breakpoints in zones 1 and 2 were grouped as "5"', and those in zones 3, 4 and 5 as "3'". Thus we subdivided patients with bcr rearrangements into those with genomic breaks at either 5' or 3' of the Bam HI site, just upstream of exon 3. Nine patients had 5' breakpoints and 13 patients had 3' breakpoints. The platelet counts of 3' patients were significantly higher than those of 5' patients (1395 vs 274 x 10(9)/l; p less than 0.03). The megakaryocyte counts from bone marrow histological sections in 3' patients (n = 12) and 5' patients (n = 7) were 63.4/mm2 and 19.5/mm2, respectively, with a significant difference of p less than 0.006. The mean number of megakaryocyte progenitor cells assayed by in vitro cloning was 128.3/2 x 10(5) bone marrow cells for 3' patients (n = 7) compared with 46.3 for 5' patients (n = 4). These results suggest that Philadelphia-positive CML patients with 3' breakpoints have higher thrombopoietic activity than patients with 5' breakpoints.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Inokuchi K,Futaki M,Yamada T,Tanabe Y,Dan K,Shinohara T,Kuriya S,Nomura Tdoi
10.1016/0145-2126(91)90113-8subject
Has Abstractpub_date
1991-01-01 00:00:00pages
1067-73issue
11eissn
0145-2126issn
1873-5835journal_volume
15pub_type
杂志文章abstract：:Mantle cell lymphoma (MCL) an incurable B-cell, non-Hodgkin lymphoma (NHL) urgently requires new treatments. We assessed reovirus mediated oncolysis in a panel of human MCL cell lines. In vitro, we found the cytopathic effect of reovirus infection ranged from high to very limited and correlated with levels of Ras acti...
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更新日期：1985-01-01 00:00:00
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更新日期：1998-09-01 00:00:00
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更新日期：2005-09-01 00:00:00
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doi：10.1016/0145-2126(93)90159-i
更新日期：1993-12-01 00:00:00
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doi：10.1016/0145-2126(85)90037-2
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更新日期：1995-12-01 00:00:00
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pub_type： 临床试验,杂志文章,多中心研究
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pub_type： 杂志文章
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更新日期：2011-07-01 00:00:00
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更新日期：2012-09-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2011.07.030
更新日期：2012-01-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/j.leukres.2008.03.036
更新日期：2008-12-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章,多中心研究
doi：10.1016/j.leukres.2011.03.006
更新日期：2011-08-01 00:00:00
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journal_title：Leukemia research
pub_type： 杂志文章
doi：10.1016/0145-2126(91)90145-j
更新日期：1991-01-01 00:00:00
