Abstract:
:Chronic myeloid leukemia (CML) is a clonal myeloproliferative neoplasm of the hematopoietic stem cell characterized by presence of the oncoprotein BCR-ABL1, which have constitutive tyrosine kinase activity. BCR-ABL1 activation induces aurora kinase A (AURKA) and aurora kinase B (AURKB) expression, which are serine-threonine kinases that play an important function in chromosome alignment, segregation and cytokinesis during mitosis. Acquisition of resistance to tyrosine kinase inhibitors has emerged as a problem for CML patients and the identification of novel targets with an important contribution for CML phenotype is of interest. In the present study, we explored the cellular effects of reversine, an AURKA and AURKB inhibitor, in the BCR-ABL1+ K562 cells. Our results indicate that reversine reduces AURKA and AURKB expression, leads to reduction of cell viability and increased apoptosis in a dose- and time-dependent manner, as well as, induces mitotic catastrophe in K562 cells. Our preclinical study establishes that reversine presents an effective antileukemia activity against K562 cells and provide new insights on anticancer opportunities for CML.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Rodrigues Alves AP,Machado-Neto JA,Scheucher PS,Paiva HH,Simões BP,Rego EM,Traina Fdoi
10.1016/j.leukres.2016.06.011subject
Has Abstractpub_date
2016-09-01 00:00:00pages
26-31eissn
0145-2126issn
1873-5835pii
S0145-2126(16)30143-6journal_volume
48pub_type
杂志文章abstract::During monocyte-macrophage differentiation of HL-60 cells by 12-O-tetradecanoyl phorbol 13-acetate, the intracellular globular(G)-actin and polymerized(F)-actin increased 3-fold and 1.7-fold, respectively. Time course studies showed that these changes of actin levels were nearly coincident with the development of macr...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(89)90121-5
更新日期:1989-01-01 00:00:00
abstract::K-562 is described as a pluripotent leukemic cell line which expresses a diversity of hematopoietic cell differentiation antigens. With the use of monoclonal antibodies (MoAb), we show that the expression of these antigens is either "modulated", i.e. they are not expressed in early logarithmic growth but are expressed...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(87)90181-0
更新日期:1987-01-01 00:00:00
abstract::Anti-MY9 is an IgG2b murine monoclonal antibody selected for reactivity with immature normal human myeloid cells. The MY9 antigen is expressed by blasts, promyelocytes and myelocytes in the bone marrow, and by monocytes in the peripheral blood. Erythrocytes, lymphocytes and platelets are MY9 negative. All myeloid colo...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(84)90001-8
更新日期:1984-01-01 00:00:00
abstract::A combination of monosomy 7 and translocation t(9;22) (q34;q11), rarely observed in acute lymphoblastic leukaemia (ALL), is here reported: a peculiarity of this case was that the "breakpoint cluster region" on chromosome 22 was not rearranged, as demonstrated by molecular analysis, and a new c-abl protein (p190) was f...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(87)90114-7
更新日期:1987-01-01 00:00:00
abstract::To determine the incidence of haematological malignancies in French Polynesia from 1990 to 1995, we collected cases from the local cancer registry, sanitary evacuation files and all the histopathology and clinical biology laboratories. All leukaemias, non Hodgkin's lymphomas, and multiple myelomas incidence was slight...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(98)00186-6
更新日期:1999-04-01 00:00:00
abstract::We examined polymorphisms of glutathione S-transferase (GST) genes in 159 Japanese patients with myelodysplasia and compared the incidence with that in 43 normal individuals to clarify their pathogenetic significance in myelodysplasia. In individuals with the GSTT1 null genotype, the odds ratios for disease risk were ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(99)00119-8
更新日期:1999-11-01 00:00:00
abstract::The concurrent development of chronic myeloid (CML) or myelomonocytic (CMML) leukemia in patients with systemic mastocytosis (SM) is a well recognized phenomenon. Although the leukemia often resembles CML in morphological and in clinical terms, a Ph-Chromosome-positive variant has not been reported in SM so far. We he...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2005.04.004
更新日期:2005-10-01 00:00:00
abstract::In order to establish diagnostic criteria for hypocellular acute leukemia (HL), we have reviewed 32 cases selected on the basis of hypothetical 40% or less cellularity, by focusing on morphology, immunophenotype, karyotype and response to low dose Ara-C (LDAC) regimen and compared them with 40 cases of myelodysplastic...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(95)00136-0
更新日期:1996-07-01 00:00:00
abstract:OBJECTIVE:Bortezomib-dexamethasone-thalidomide has been reported to be effective in newly-diagnosed multiple myeloma (MM) with an overall response rate of 92% and a CR rate of 18% (Alexanian et al, Hematology 2007;12(3):235-9), but this regimen has not been tested in the Chinese patients. We report here our results tes...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2009.04.006
更新日期:2009-12-01 00:00:00
abstract::With Percoll density gradients, blasts from peripheral blood and bone marrow could be separated with a significant enrichment, and very often with a high degree of purity. This allowed a study of selected cases, where the separated sample exhibited chromosome abnormalities and/or an abnormal DNA content distribution (...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(85)90037-2
更新日期:1985-01-01 00:00:00
abstract::It has been suggested that acidic isoferritins play a major role in the regulation of human granulocyte-macrophage (CFU-GM) proliferation. Such regulatory isoferritins are said to occur in some human tissues including both leukaemic and normal leucocytes, and certain established cell lines. They are glycosylated and b...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(84)90065-1
更新日期:1984-01-01 00:00:00
abstract::Chronic Lymphocytic Leukemia B cells (CLL) are malignant cells which retain at least some functions of normal B cells. Paramount amongst the latter is that when such cells are appropriately stimulated, they are able to present antigens, including any potential tumor antigens, making them excellent choices as a candida...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2018.03.002
更新日期:2018-05-01 00:00:00
abstract::Changes of phosphoprotein patterns in HL-60 cells were studied during short exposures to 1 alpha, 25-dihydroxyvitamin D3 [1,25(OH)2D3]. One hundred nanometers 1,25(OH)2D3 dephosphorylated at least three proteins in 6 h: phosphoproteins with molecular weights of 82 kD (pp82), 33 kD (pp33), and 31 kD (pp31). Phosphoryla...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(91)90169-t
更新日期:1991-01-01 00:00:00
abstract::We evaluated the cytotoxicity of dimethylsphingosine (DMS) against four human leukemia cell lines: two acute (HL60 and a multi-drug resistance MDR-positive derivative HL60-dox) and two blast crisis chronic myelogenous leukemias (JFP1, from a treatment refractory patient and K562), and against blasts isolated from 11 l...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(01)00129-1
更新日期:2002-03-01 00:00:00
abstract::Model cell lines are essential tools for investigating the biology and therapeutics of cancer. Approximately 1500 human hematopoietic neoplastic cell lines have been described, covering most major disease entities. Waldenström's macroglobulinemia (WM) is a rare incurable hematological neoplasm from which only three ce...
journal_title:Leukemia research
pub_type: 杂志文章,评审
doi:10.1016/j.leukres.2008.04.016
更新日期:2008-11-01 00:00:00
abstract::We investigated Syk as a potential marker of CML progression. We observed a significant over-expression of Syk mRNA and constitutive phosphorylation of Syk Y348 in blast cells from six AP or BP-CML, but not in 15 CML in chronic phase. We could follow in vivo the recurrence of pSyk(348) throughout blast cell escape, de...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2014.11.014
更新日期:2015-03-01 00:00:00
abstract::microRNA profiling of acute myeloid leukemia patient samples identified miR-125a as being decreased. Current literature has investigated miR-125a's role in normal hematopoiesis but not within acute myeloid leukemia. Analysis of the upstream region of miR-125a identified several CpG islands. Both precursor and mature m...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2013.12.021
更新日期:2014-03-01 00:00:00
abstract::Hematopoiesis is regulated by several cytokines with pleiotropic activity. Several evidences have clearly demonstrated that these molecules, formerly regarded as specific for the hematopoietic system, also affect certain endothelial cell functions and that hematopoietic factors clearly influence angiogenesis. This rev...
journal_title:Leukemia research
pub_type: 杂志文章,评审
doi:10.1016/j.leukres.2012.02.003
更新日期:2012-05-01 00:00:00
abstract::Cases of leukemia associated with Turner syndrome (TS) are rare. Here we report three TS patients with leukemia including one case of T-large granular lymphocyte leukemia (T-LGL), one rare case of coexistence of chronic lymphocytic leukemia (CLL) and idiopathic myelofibrosis (IMF) and one case of a patient with AML-M2...
journal_title:Leukemia research
pub_type: 杂志文章,评审
doi:10.1016/j.leukres.2007.06.004
更新日期:2008-03-01 00:00:00
abstract::Replication of the Friend spleen focus forming virus (SFFV) in C3H/HeJ or C57BL/6J mouse continuous bone marrow cultures is associated with an increased cumulative production of pluripotential/hematopoietic stem cells (CFUs), granulocyte-macrophage progenitor cells (GM-CFUc), and total granulocytes, compared to uninfe...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(83)90133-9
更新日期:1983-01-01 00:00:00
abstract::The ability of pH-sensitive liposomes and immunoliposomes to deliver synthetic antisense oligonucleotides (oligos) into human myeloid and lymphoid leukaemia cells was examined. The cellular uptake of an 18mer anti-myb oligonucleotide encapsulated in liposomes was from three- to five-fold higher than that of 32P-oligos...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(96)00062-8
更新日期:1996-11-01 00:00:00
abstract::We compared 2-[fluorine-18] fluoro-2-deoxy-d-glucose PET-CT and contrast-enhanced computed tomography (CECT) in 62 consecutive patients with newly diagnosed Hodgkin Lymphoma (HL), aiming to provide evidences that may spare CECT from the staging procedures of HL patients. Among a total of 1448 nodal sites examined, dis...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2018.11.018
更新日期:2019-01-01 00:00:00
abstract::Defective apoptosis is a hallmark of the progression of B chronic lymphocytic leukaemia (B-CLL). Smac-mimetics have been shown to induce apoptosis in several tumours. We describe the in vitro pro-apoptotic activity and regulation of the molecular pathway induced by new Smac-mimetics in B-CLL. The cytotoxic effect was ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2013.03.016
更新日期:2013-07-01 00:00:00
abstract::FcRIII (CD16) expression on neutrophils from 17 patients with chronic myeloid leukemia (CML) was studied by flow cytometry using monoclonal antibodies. A variable proportion of CD16-negative neutrophils were found both in CML patients in chronic phase (3 out of 8 patients) and in CML patients in hematological remissio...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(92)90120-v
更新日期:1992-12-01 00:00:00
abstract::Aneuploidy is a very common prognostic factor in multiple myeloma (MM). Nonhyperdiploidy including near-tetraploidy (NT) is a poor prognostic indicator, compared to hyperdiploidy in multiple myeloma (MM). NT results from endoduplication of hypodiploidy. We report of a 55-year-old female patient diagnosed with advanced...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2010.01.013
更新日期:2010-07-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF) is pathognomonically elevated in patients with POEMS (polyneuropathy, organomegaly, endocrinopathy, monoclonal gammopathy and skin changes) syndrome. However, its source of overproduction is unclear. As clinical improvement is almost always associated with VEGF reduction after...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2016.09.017
更新日期:2016-11-01 00:00:00
abstract::We report on four cases of trisomy 14 as the sole anomaly. Three cases were myelodysplastic syndromes and one was a non-Hodgkin's lymphoma. This anomaly is mainly in myeloid disorders and still remains to be well documented. On the other hand, we show this anomaly to be also a non-random anomaly in lymphoproliferative...
journal_title:Leukemia research
pub_type: 杂志文章,评审
doi:10.1016/0145-2126(92)90181-6
更新日期:1992-01-01 00:00:00
abstract::Mutation of the tumor suppressor PTEN results in loss of its PI3-kinase counteracting function. PI3-kinase stimulates tumor formation by PKB/Akt-mediated cell proliferation and prevention of apoptosis. PI3-kinase may also activate Rho-GTPases and their regulatory GEFs to promote invasion. Here we have analyzed the fun...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2007.03.034
更新日期:2008-01-01 00:00:00
abstract::Over-expression of the Flt3 is prevalent in acute myeloblastic leukemia (AML), playing a role in leukemogenesis while decreased expression of PU.1 induces AML in mice model. Therefore, we speculated that there is an inverse relationship between these two factors. To clarify this, we measured the expression level of Fl...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2005.07.015
更新日期:2006-06-01 00:00:00
abstract:BACKGROUND:The skin is the common extranasal sites in extranodal natural killer (NK) / T - cell lymphoma, (ENKTL). However, data regarding cutaneous ENKTL are relatively scarce. Thus, we performed a large retrospective study to analyze clinical features and treatment outcomes of cutaneous ENKTL. PATIENTS AND METHODS:S...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2019.106284
更新日期:2020-01-01 00:00:00