Abstract:
:We have utilized the MTT assay to measure the metabolic activity of cells from the bone marrow of 55 patients with acute myeloid leukaemia (AML), myelodysplastic syndrome (MDS) and non-clonal disease. Doubling dilutions of cells were exposed to MTT for 3-4 h. The mean optical density of the formazan produced by each cell dilution was plotted and the gradient of the line produced was calculated, higher gradients indicating more metabolically active cells. Results showed that the median activity of mononuclear cells from seven patients with non-clonal disease was 0.202 (range 0.175-0.253); blast cells from 27 patients with de novo AML had a median activity of 0.187 (range 0.079-0.345) and 13 patients with MDS a median of 0.155 (range 0.062-0.311). Seven assays on mononuclear cells from five patients in remission had a median activity of 0.203 (range 0.190-0.248), indicating no significant difference between these and normal patients. There was no correlation between the metabolic activity of cells when compared with their proliferative capacity, cell size and expression of P-glycoprotein. Following exposure of the AML patients' blast cells to the anthracyclines, cytosine arabinoside, 6-thioguanine and etoposide, cell survival was measured using the MTT assay. While there was no correlation between the in vitro sensitivity of these cells to the anthracyclines or etoposide, less metabolically active cells showed significantly greater sensitivity to 6-thioguanine. Conversely, the more active cells appeared to be more sensitive to cytosine arabinoside. Patients whose blasts cells showed higher metabolic activity appeared to achieve remission and had a longer mean survival time. Therefore, by using a simple technique we were able to establish that some patients were more likely to respond to certain cytotoxic regimes. Our preliminary study reflected the multifactorial nature of clinical response in AML and MDS, so providing further information on the relationship between cellular metabolic activity and treatment failure.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Elgie AW,Sargent JM,Taylor CG,Williamson Cdoi
10.1016/0145-2126(95)00163-8subject
Has Abstractpub_date
1996-05-01 00:00:00pages
407-13issue
5eissn
0145-2126issn
1873-5835pii
0145212695001638journal_volume
20pub_type
杂志文章abstract::Imatinib is a highly effective drug for the treatment of chronic myeloid leukemia (CML) that targets the BCR-ABL kinase. However, a number of patients have CML that is resistant to Imatinib treatment. In this report, we developed AGM130 as a potential therapeutic drug for Imatinib-resistant CML treatment. The AGM130 c...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2012.12.017
更新日期:2013-04-01 00:00:00
abstract::Aggressive chemotherapy of myelodysplastic syndromes is rarely feasible because these disorders predominantly occur in elderly patients who often have concurrent illnesses. Alternative treatment modalities must therefore be evaluated. This review summarizes the results that have been obtained with low-dose chemotherap...
journal_title:Leukemia research
pub_type: 杂志文章,评审
doi:10.1016/0145-2126(92)90058-f
更新日期:1992-01-01 00:00:00
abstract::The colony-forming capacity of the peripheral blood stem/progenitor cells (PBSC) in different forms of myelodysplastic syndrome (MDS) was investigated. In most cases of refractory anemia (RA) the colony growth of PBSC was definitely reduced as compared to the controls. However, in RA with unfavorable chromosomal aberr...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(01)00064-9
更新日期:2001-11-01 00:00:00
abstract::Despite advances in understanding the complexities of acute myeloid leukaemia (AML), the treatment of refractory or relapsed AML (rrAML) remains a daunting clinical challenge. Numerous clinical trials have failed to identify new treatments or combinations of existing therapies that substantially improve outcomes and s...
journal_title:Leukemia research
pub_type: 杂志文章,评审
doi:10.1016/j.leukres.2016.05.025
更新日期:2016-08-01 00:00:00
abstract::A 53-year-old male patient was admitted to our hospital with painful splenomegaly. He was diagnosed as having chronic myelomonocytic leukemia (CMML) with leukocytosis, monocytosis, increased lysozyme concentrations in serum und urine, and lack of the Philadelphia chromosome. The clinical course of the disease was char...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(96)00117-8
更新日期:1997-03-01 00:00:00
abstract::In a recent phase I study, a combination of gemcitabine at 10 mg/(m(2)min) for 12 h and mitoxantrone 12 mg/m(2) daily for 3 days, achieved a complete remission (CR) in 3 of 12 (25%) patients with refractory leukemia. A pilot assessment of this regimen was conducted to determine its tolerability in patients with refrac...
journal_title:Leukemia research
pub_type: 临床试验,杂志文章
doi:10.1016/s0145-2126(02)00171-6
更新日期:2003-04-01 00:00:00
abstract::We describe changes in antigen expression on HL60 cells with differentiation into granulocytes induced by all-trans retinoic acid (ATRA) or dimethylsulphoxide (DMSO), into monocytes by alpha1,25-dihydroxyvitamin D3 (D3), or into macrophages by 12-O-tetradecanoyl phorbol-13-acetate (TPA). Undifferentiated cells express...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(98)00041-1
更新日期:1998-06-01 00:00:00
abstract::Thermal stress induces expression of a family of heat shock proteins which may regulate the synthesis of various cellular genes. We investigated the effect of heat shock on polyadenylation in Epstein-Barr Virus (EBV) negative and EBV transformed human Burkitt's lymphoma (BL) B-cell lines. Incubation of the BL B-cell l...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(95)00063-1
更新日期:1995-11-01 00:00:00
abstract::The incidence of B cell chronic lymphocytic leukemia (B-CLL) is lower in Asian countries, including India, when compared with Western countries. The bcl-2 gene rearrangement was restricted to the 5' region and seen in only one of the 20 Indian patients analyzed. The incidence remains similar to that reported from West...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(96)00036-7
更新日期:1996-09-01 00:00:00
abstract::Early T-cell precursor-ALL (ETP-ALL) is a subtype of T-ALL with a poor prognosis in children. We analyzed ETP-ALL compared to conventional T-ALL/LBL in both adults and children to determine any differences in clinical outcomes, based on the following parameters: induction failure, relapse, and survival. Patients with ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2013.06.010
更新日期:2013-09-01 00:00:00
abstract::Outcome of adult acute lymphoblastic leukemia (ALL) with central nervous system (CNS) involvement is not clearly defined. We studied 104 patients presenting with CNS involvement at diagnosis among 1493 patients (7%) included into the LALA trials, and 109 patients presenting CNS disease at the time of first relapse amo...
journal_title:Leukemia research
pub_type: 杂志文章,多中心研究
doi:10.1016/j.leukres.2008.04.011
更新日期:2008-11-01 00:00:00
abstract::Determination of T-cell clonality has an important additional value for diagnosis of T-cell lymphomas. Various molecular methods have been developed, including polymerase chain reaction (PCR) of T-cell receptor γ (TCRγ). The detection of PCR products usually relies commonly on either GeneScan (GS) analysis or heterodu...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2012.06.003
更新日期:2012-09-01 00:00:00
abstract::This study examined the immunoregulatory role of recombinant interleukin 4 (IL-4), also known as B-cell stimulating factor 1, on the generation of cytotoxic effector cells from normal and leukaemic human blood mononuclear cells. When tested on cells from normal individuals, the addition of IL-4 to mixed lymphocyte cul...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(89)90066-0
更新日期:1989-01-01 00:00:00
abstract::A diagnosis of myelodysplastic syndrome was made in an 18-year-old patient with Warkany syndrome due to constitutional trisomy 8 mosaicism. The possible causal role of this particular chromosome constitution with respect to myelodysplasia and embryonal childhood tumors is discussed. ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(95)00050-x
更新日期:1995-10-01 00:00:00
abstract::To characterize the molecular mechanisms involved in the transition from the chronic phase to blast crisis in chronic myelogenous leukemia (CML), gene expression profiles of leukemic cells from patients in the chronic and blast crisis phases were analyzed using an 8.7K cDNA chip and real-time PCR. A transient transfec...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2009.11.008
更新日期:2010-08-01 00:00:00
abstract::We evaluated the effect of SCF on myeloid differentiation by correlating clonogenic potential (as CFU-GM), bone marrow (BM) plasma SCF levels and CD34/c-kit expression in 57 MDS samples. There was a significant correlation between low SCF levels and 'leukemic' in vitro growth, the number of clusters and the colony/clu...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(98)00175-1
更新日期:1999-03-01 00:00:00
abstract::When exposed to the anthracycline doxorubicin, K562 cells undergo differentiation which is characterized by arrested cell division, an increased mean cell diameter, and the production of hemoglobin. The influence of expression of p28, a low-molecular weight stress protein, on the differentiation of K562 cells was exam...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(00)00095-3
更新日期:2001-02-01 00:00:00
abstract::miRNAs have been reported to be involved in the pathogenesis of many cancers. In this article, we investigated the role and the mechanisms of miR-15a/16 in the pathogenesis of multiple myeloma (MM). We found that miR-15a/16 was down-regulated in bone marrow-derived mononuclear cells (BM-MNCs) of newly diagnosed patien...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2016.08.013
更新日期:2016-10-01 00:00:00
abstract::We wish to demonstrate new evidence for the in vivo production of interleukin-3 (IL-3). Syngeneic murine splenocytes were transplanted into lethally irradiated mice. The spleen cells in these transplant mice spontaneously produced IL-3 in cultures, and IL-3-like activity was detected in the serum. Chronological change...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(89)90138-0
更新日期:1989-01-01 00:00:00
abstract::Chlorambucil-induced apoptosis was assessed by three different flow cytometric methods in B-cell chronic lymphocytic leukaemia (B-CLL) cells cultured in vitro and the results were compared with those derived from the morphological assessment of the same samples. Spontaneous apoptosis was consistently observed in the c...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(98)00013-7
更新日期:1998-05-01 00:00:00
abstract::With the application of adequately sensitive tests, it is now becoming evident that more than 90% of patients with conventionally-defined polycythemia vera (PV) carry the somatic JAK2V617F mutation in their granulocytes. However, the specific mutation is also found in other classic and atypical myeloproliferative diso...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2006.01.004
更新日期:2006-06-01 00:00:00
abstract::The functional behavior of hematopoietic stem cell (HSC) and progenitors in chronic myelomonocytic leukemia (CMML) and on disease progression is little known. We performed cell proliferation, apoptosis, hematopoietic colony forming/replating and differentiation potential studies in the purified subpopulations of Lin(-...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2008.02.011
更新日期:2008-09-01 00:00:00
abstract::Experimental data are in keeping with the finding that megakaryocytes isolated from normal human bone marrow may promote fibroblast growth. This effect can be significantly enhanced by interleukin (IL)-3. In this context it has been demonstrated that IL-3 induces the release of platelet-derived growth factor (PDGF) an...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(99)00091-0
更新日期:1999-08-01 00:00:00
abstract::Mantle cell lymphoma (MCL) is a distinct type of B cell malignancy and accounts for approximately 5-10% of non-Hodgkin's lymphomas (NHL). The characteristic cytogenetic aberration in MCL is the translocation (11;14)(q13;q32) present in virtually all cases. This rearrangement at the BCL1 locus at 11q13 dysregulates the...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/s0145-2126(02)00026-7
更新日期:2002-09-01 00:00:00
abstract::Infant leukemia cells with 46XY,t(11; 17)(q23; p13) karyotype and a hybrid pre B myeloid phenotype (HLA-DR, (Ia), B4 and My7-positive and CALLA and T11-negative) and immunoglobulin heavy chain gene rearrangement were maintained in long-term culture for over 10 months. The in-vitro survival and growth of the leukemia c...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(86)90253-5
更新日期:1986-01-01 00:00:00
abstract::Myelodysplastic syndrome (MDS) are a heterogeneous group of clonal disease characterized by insufficiency of bone marrow, increase of apoptosis and increased risk of acute leukemia progression. Proteins related to the mitotic spindle (AURKA, AURKB, TPX2), to the mitotic checkpoint (MAD2, CDC20) and the regulation of t...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2017.11.013
更新日期:2018-01-01 00:00:00
abstract::The effect of VM-26, a topoisomerase II targeting drug, on IW32 murine erythroleukemia cells was investigated. The VM-26 induced IW32 cells to differentiate at a non-toxic but cytostatic concentration (0.01 microgram/ml). More than 40% of the cells were induced to synthesize hemoglobin, and cells were arrested in G2/M...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(95)00144-1
更新日期:1996-03-01 00:00:00
abstract::Epstein-Barr virus (EBV) can induce a broad spectrum of hematological diseases, especially in immune deficient patients. We assayed for receptor for EBV (EBVR) using fluoresceinated viral particles on 44 human hematopoietic cell lines derived from patients with T, B, and non-T, non-B acute lymphocytic leukemia (ALL), ...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/0145-2126(85)90085-2
更新日期:1985-01-01 00:00:00
abstract::The peripheral blood lymphocyte to monocyte ratio (LMR) at diagnosis has been used to predict survival in diffuse large B-cell lymphoma (DLBCL) patients, but its prognostic significance with respect to different cell-of-origin (COO) subtypes remains unknown. We retrospectively analyzed 168 de novo DLBCL patients in th...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2014.03.013
更新日期:2014-06-01 00:00:00
abstract::We investigated protracted low-dose oral Clofarabine for the treatment of myelodysplastic syndromes (MDS). Adults with an International Prognostic Scoring System (IPSS) score of INT-1 or higher who had failed first line therapy were eligible. INT-1 patients had to be transfusion-dependent. We started with oral Clofara...
journal_title:Leukemia research
pub_type: 杂志文章
doi:10.1016/j.leukres.2015.05.004
更新日期:2015-08-01 00:00:00